##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920805_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3535066
Sequences flagged as poor quality	0
Sequence length	101
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.801467921673883	33.0	31.0	34.0	30.0	34.0
2	31.854874845335278	33.0	31.0	34.0	30.0	34.0
3	31.965177453546836	34.0	31.0	34.0	30.0	34.0
4	35.52638253430063	37.0	35.0	37.0	33.0	37.0
5	35.35041382537129	37.0	35.0	37.0	33.0	37.0
6	35.3505363124762	37.0	35.0	37.0	33.0	37.0
7	35.28753748869187	37.0	35.0	37.0	33.0	37.0
8	35.256880918206335	37.0	35.0	37.0	33.0	37.0
9	36.831657457031916	39.0	37.0	39.0	33.0	39.0
10-11	36.66840972700368	39.0	37.0	39.0	32.5	39.0
12-13	36.51600931920366	39.0	37.0	39.0	32.0	39.0
14-15	37.72824552639187	40.0	38.0	41.0	33.0	41.0
16-17	37.625827778038655	40.0	38.0	41.0	32.0	41.0
18-19	37.57179936668792	40.0	37.5	41.0	32.0	41.0
20-21	37.50571036014603	40.0	37.5	41.0	32.0	41.0
22-23	37.348000009052164	40.0	37.0	41.0	32.0	41.0
24-25	37.213125865259656	40.0	37.0	41.0	32.0	41.0
26-27	37.17262124667545	40.0	37.0	41.0	31.5	41.0
28-29	37.16132640805009	40.0	37.0	41.0	31.5	41.0
30-31	36.95310497738939	40.0	36.0	41.0	31.0	41.0
32-33	36.71225445295788	40.0	36.0	41.0	30.5	41.0
34-35	36.480697107211014	40.0	35.5	41.0	30.0	41.0
36-37	36.33658706796422	39.0	35.0	41.0	30.0	41.0
38-39	36.38626930869184	39.5	35.0	41.0	30.0	41.0
40-41	36.253659620499306	39.0	35.0	41.0	30.0	41.0
42-43	36.08468851783814	39.0	35.0	41.0	29.5	41.0
44-45	35.886481751684414	39.0	35.0	41.0	28.5	41.0
46-47	35.60766828681558	39.0	35.0	41.0	27.5	41.0
48-49	35.30863737763312	39.0	35.0	40.5	26.5	41.0
50-51	35.037976518684516	38.5	35.0	40.0	26.0	41.0
52-53	34.77493277353238	38.0	34.0	40.0	25.5	41.0
54-55	34.49225106405368	38.0	34.0	40.0	24.5	41.0
56-57	34.21712494193885	38.0	34.0	40.0	24.0	41.0
58-59	33.8429730873483	37.0	33.5	40.0	23.0	41.0
60-61	33.37193421565538	36.5	33.0	39.5	22.0	41.0
62-63	32.92874022719802	36.0	32.5	39.0	20.5	40.5
64-65	32.49860822966247	35.5	32.0	39.0	19.5	40.0
66-67	32.0597126050829	35.0	32.0	38.0	18.0	40.0
68-69	31.60477923184461	35.0	31.0	37.0	16.5	39.5
70-71	31.137524589357028	35.0	31.0	37.0	12.5	39.0
72-73	30.641618996646738	34.5	30.5	36.0	8.0	39.0
74-75	30.234216136275812	34.0	30.0	35.5	7.0	37.5
76-77	28.898692131914935	32.5	28.5	34.5	4.5	36.0
78-79	29.7366855385444	34.0	30.0	35.0	4.0	36.0
80-81	29.85758978757398	34.0	30.0	35.0	2.0	36.0
82-83	29.525892161560776	34.0	30.0	35.0	2.0	36.0
84-85	29.204816119416158	34.0	30.0	35.0	2.0	35.5
86-87	28.821679849824584	34.0	29.0	35.0	2.0	35.0
88-89	28.39353070069979	34.0	29.0	35.0	2.0	35.0
90-91	28.089279521231006	34.0	29.0	35.0	2.0	35.0
92-93	27.74066806673482	34.0	27.5	35.0	2.0	35.0
94-95	27.51379776219171	33.5	27.0	35.0	2.0	35.0
96-97	27.09507813998381	33.0	27.0	35.0	2.0	35.0
98-99	26.515162093154697	33.0	25.5	35.0	2.0	35.0
100-101	24.079439110896374	30.0	13.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	26941.0
3	14739.0
4	9172.0
5	6312.0
6	5357.0
7	5791.0
8	6442.0
9	7684.0
10	9107.0
11	11945.0
12	16584.0
13	21051.0
14	19097.0
15	19271.0
16	22614.0
17	26945.0
18	25085.0
19	20893.0
20	19848.0
21	20351.0
22	21493.0
23	23740.0
24	27494.0
25	31880.0
26	37730.0
27	45423.0
28	54607.0
29	65940.0
30	79848.0
31	99605.0
32	126045.0
33	168905.0
34	261438.0
35	338031.0
36	541175.0
37	843235.0
38	434052.0
39	19195.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.33434629412409	14.693890058853873	11.774491661091126	25.197271985930914
2	19.02100271960976	17.761931460402717	37.57893063382692	25.6381351861606
3	20.23935881303919	27.40080182316891	28.883258122335175	23.476581241456724
4	12.420477691307712	15.24140089430586	35.8734536267719	36.46466778761452
5	12.285322132781754	34.0570637476436	28.781147950928187	24.876466168646452
6	28.15877422654313	31.678812149674034	23.740978672769707	16.421434951013126
7	28.640579691264445	32.2167226520207	21.000440953694767	18.14225670302009
8	32.90376595583179	28.76408650561761	19.86153391240904	18.470613626141564
9	32.62068856143404	18.075473963714135	19.681785021659145	29.622052453192683
10-11	29.250203564322785	22.434077021659242	26.824849716426048	21.490869697591922
12-13	27.08613542303022	21.703418471545213	27.481805357184836	23.72864074823973
14-15	25.588089227418955	22.239009318089096	25.863336864618297	26.30956458987366
16-17	25.823041409176923	22.624618621286675	26.38845627010734	25.163883699429068
18-19	24.80251324903949	23.736717351465707	27.104343703787563	24.35642569570724
20-21	25.55679991713061	23.712743800291573	26.24125067112033	24.489205611457486
22-23	25.08346566630068	22.637465514281132	27.93456818310174	24.34450063631645
24-25	22.947561818372492	24.283470713784414	27.21294849729709	25.556018970546006
26-27	23.487446080538785	23.4890310105712	26.36938662358676	26.654136285303252
28-29	24.63431484641047	24.99181864138654	25.53817634863666	24.835690163566333
30-31	26.371592751764368	24.46614814330666	26.91930179581792	22.242957309111056
32-33	26.005939703744822	24.27696867989009	25.996121310073644	23.720970306291438
34-35	26.018220588455804	23.56491151830787	26.205513367526994	24.21135452570933
36-37	25.09099336200488	24.35271836921333	27.29867356111927	23.257614707662512
38-39	26.705013766871172	25.037936352943476	25.167555172883098	23.089494707302254
40-41	26.229222253297213	23.508326093897498	26.403251641940827	23.85920001086446
42-43	23.705094380645097	24.828614548627804	27.229672984549104	24.236618086177995
44-45	23.942379467223994	24.777080931676068	26.190896594188256	25.08964300691168
46-47	24.21027210404102	22.987246276998576	26.247992972556727	26.55448864640368
48-49	23.8234235793926	23.4463516569439	26.926448697907386	25.803776065756118
50-51	23.76062645517015	25.17273912883578	26.412409719334278	24.654224696659792
52-53	25.31131800186736	23.348054509133913	25.116490974857918	26.2241365141408
54-55	23.46174747218434	23.154545343962	28.077459802334896	25.306247381518766
56-57	24.185559955774334	23.450747464358017	26.29333688632259	26.07035569354506
58-59	25.4432227251389	24.784886078552596	25.92772851155664	23.844162684751865
60-61	26.62570442169875	25.053647658868506	25.518532737590878	22.80211518184187
62-63	28.000044695563105	24.64139943505374	24.939332137962754	22.419223731420402
64-65	26.831784922413494	23.318468739374183	25.248909342006925	24.6008369962054
66-67	24.93253879258199	24.844104510926588	26.255883532758535	23.96747316373289
68-69	24.12129201029684	23.355185365342095	27.01476975828976	25.508752866071305
70-71	23.77027739537708	23.236073324923424	27.051471094821967	25.94217818487753
72-73	22.6015505319386	25.830676977134797	25.318723834083773	26.249048656842827
74-75	23.751111634584866	24.05175198706894	25.296740252794407	26.900396125551783
76-77	23.173497167389133	22.952147584543596	27.059780840368177	26.814574407699098
78-79	22.665808660221803	24.91864179429418	26.9367782688446	25.478771276639417
80-81	25.062525675135834	23.6201064464254	25.967082636874643	25.350285241564123
82-83	25.20056528344806	23.912126433367	26.465224316807706	24.422083966377233
84-85	23.96017722273161	25.276319954871063	25.44908976872835	25.31441305366898
86-87	23.91875701002784	25.361283461258594	24.909481631690035	25.81047789702353
88-89	22.824212929065485	24.48648349063425	26.30061950103958	26.38868407926068
90-91	24.837156066448127	24.869175124100448	26.290710628724064	24.002958180727365
92-93	25.095227946227954	25.51512367162683	25.137556201493265	24.25209218065195
94-95	24.16372404594378	23.79049624855639	25.347849240694238	26.697930464805598
96-97	22.92565671813215	25.247063163415582	25.494013170289847	26.33326694816242
98-99	23.026172106623456	25.175269998687348	25.108364414489852	26.69019348019934
100-101	23.108343180518784	24.332539051858195	25.22587232106813	27.333245446554887
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1096.0
1	916.0
2	3737.5
3	6154.0
4	3730.0
5	1873.5
6	2137.5
7	3607.0
8	5501.5
9	5596.0
10	4289.5
11	3276.5
12	3052.5
13	3263.5
14	3658.0
15	4009.5
16	4138.0
17	4355.0
18	4697.0
19	4835.5
20	4972.5
21	5157.0
22	5506.5
23	6218.0
24	6648.5
25	7295.0
26	8287.0
27	9396.0
28	10585.5
29	12234.5
30	14643.0
31	17982.0
32	21864.5
33	25130.0
34	28625.5
35	32911.0
36	37469.0
37	40638.0
38	41716.0
39	42477.5
40	43615.0
41	45908.5
42	48924.0
43	54240.0
44	63647.5
45	76535.5
46	90610.5
47	104388.5
48	122906.5
49	144890.5
50	166767.5
51	182920.0
52	190268.0
53	191122.0
54	195500.5
55	192719.5
56	189789.0
57	199431.0
58	196832.0
59	179448.5
60	134290.5
61	82553.5
62	57375.5
63	41224.0
64	27241.5
65	18584.5
66	13147.5
67	8933.5
68	5861.5
69	4292.5
70	3009.5
71	1717.0
72	1076.0
73	750.5
74	527.0
75	332.5
76	195.0
77	123.0
78	79.5
79	50.5
80	29.5
81	21.0
82	16.5
83	12.0
84	7.5
85	3.5
86	7.0
87	5.0
88	0.5
89	0.0
90	0.5
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.30392643305669537
2	0.0
3	0.003451137828826958
4	0.03258779326892341
5	5.65760299807698E-5
6	0.10500511164430876
7	0.11549996520574156
8	0.12395808168786664
9	0.1129823318715973
10-11	0.11996947157422239
12-13	0.11934713524443391
14-15	0.1200967676416791
16-17	0.05647702192830346
18-19	0.05066383484777936
20-21	0.050776986907740906
22-23	0.07427018335725556
24-25	0.05984329571215927
26-27	0.05055068278781782
28-29	0.03161185675175513
30-31	0.022602123977317538
32-33	0.02497831723650987
34-35	0.013945991390259756
36-37	0.02876891124522145
38-39	0.02424282884675986
40-41	0.01712839307667806
42-43	0.011781958243495313
44-45	0.02964583970992338
46-47	0.026067405813639685
48-49	0.0018104329593846336
50-51	0.007241731837538535
52-53	0.0038330260311971545
54-55	0.002743937454067336
56-57	4.101762173605811E-4
58-59	5.799043073028906E-4
60-61	0.012135558430875123
62-63	0.0011173765921202038
64-65	0.0012446726595769359
66-67	0.03871214851434174
68-69	0.06046563204194773
70-71	0.021003851130360792
72-73	0.035261010685514786
74-75	0.08783428654514512
76-77	0.27928757200007015
78-79	0.08053597867762582
80-81	0.01465319176501938
82-83	0.015728136334654006
84-85	0.008627844572067396
86-87	0.18778998751367018
88-89	0.7272707213953007
90-91	0.29992367893555594
92-93	0.18924682028567502
94-95	0.0026449294016009884
96-97	0.003960322098653886
98-99	0.006619395507750067
100-101	0.006081923222932754
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
101	3535066.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.35091890679643
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.81382449543102	27.157752407043894
2	15.943841538647458	12.22921947822962
3	5.344400446603526	6.1488800439941524
4	2.2413766900090266	3.4383542271247993
5	1.1686577984915074	2.2409550229871518
6	0.7816395510348819	1.7985957021650116
7	0.5337351729300808	1.4328464034322548
8	0.3912887769289673	1.2005027322513904
9	0.3270400746417157	1.1288058643671381
>10	2.1130184558243306	15.345308113791106
>50	0.19264718702539718	5.076710057501582
>100	0.12570684343512528	9.317557595787735
>500	0.011485585047537439	3.011509452907013
>1k	0.01029997626794212	7.744681220551711
>5k	0.0010374076816632351	2.728321677865471
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9664	0.2733753768670797	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	9513	0.2691038866035316	No Hit
TCTCTATGGTGTCACTGGGCTCGACCTCCAGGGTGATGGTCTTGCCTGTC	8790	0.2486516517654833	No Hit
CCCTCTCTGATTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTT	8138	0.23020786599175233	No Hit
GGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCAGGCA	8080	0.22856716112231004	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6378	0.1804209596086749	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	6020	0.17029385024211713	No Hit
CTCTCAGGCGAAGGACCAGGTGCAGGGTTGACTCCTTCTGGATGTTGTAA	5979	0.16913404162751136	No Hit
GGATAAAGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCAGGCA	5928	0.1676913528630017	No Hit
CCATCGAGAACGTGAAGGCCAAGATCCAGGATAAAGAGGGCATCCCCCCT	5767	0.16313698244954974	No Hit
CTTTATCCTGGATCTTGGCCTTCACGTTCTCGATGGTGTCACTGGGCTCC	5634	0.15937467645582853	No Hit
GGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGGCA	5505	0.1557255225220689	No Hit
CTGACAGGGTGCGGCCATCTTCCAGCTGCTTGCCTGCAAAGATCAGCCTC	5408	0.15298158506800155	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5202	0.14715425397998227	No Hit
CTTTATCCTGGATCTTTGCCTTGACATTCTCTATGGTGTCACTGGGCTCG	4993	0.1412420588469918	No Hit
CCATCGAGAACGTGAAGGCAAAGATCCAGGACAAGGAGGGCATCCCCCCT	4879	0.13801722513808795	No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	4831	0.13665940041854946	No Hit
GTCTGACAGGGTGCGGCCATCTTCCAGCTGCTTGCCTGCAAAGATCAGCC	4713	0.13332141464968406	No Hit
CTGACAGGGTGCGGCCATCTTCCAGCTGCTTGCCGGCAAAGATCAGCCTC	4647	0.13145440566031866	No Hit
GTCTGACAGGGTGCGGCCATCTTCCAGCTGCTTGCCGGCAAAGATCAGCC	4570	0.12927622850605902	No Hit
AGGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCAGGC	4065	0.11499078093591462	No Hit
TCTCATTACAGATGGTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACT	3669	0.10378872699972222	No Hit
TCACAAAGATCTGCATGCCACCTCTGAGGCGAAGGACCAGGTGCAGGGTG	3652	0.10330783074488567	No Hit
GGATAAAGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGGCA	3577	0.1011862296206068	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	2.8288014990384904E-5	0.0
8	0.0	0.0	0.0	2.8288014990384904E-5	0.0
9	0.0	0.0	0.0	2.8288014990384904E-5	0.0
10-11	0.0	0.0	0.0	9.900805246634717E-5	0.0
12-13	0.0	0.0	0.0	1.4144007495192452E-4	0.0
14-15	0.0	0.0	0.0	1.8387209743750188E-4	8.486404497115471E-5
16-17	0.0	0.0	0.0	2.121601124278868E-4	8.486404497115471E-5
18-19	0.0	0.0	0.0	2.545921349134641E-4	1.2729606745673206E-4
20-21	0.0	0.0	0.0	4.243202248557736E-4	1.4144007495192452E-4
22-23	0.0	0.0	0.0	8.486404497115472E-4	1.9801610493269434E-4
24-25	0.0	0.0	0.0	0.0012163846445865508	1.9801610493269434E-4
26-27	0.0	0.0	0.0	0.0014568327720048224	1.9801610493269434E-4
28-29	0.0	0.0	0.0	0.0019943050568221356	1.9801610493269434E-4
30-31	0.0	0.0	0.0	0.0036774419487500378	2.2630411992307923E-4
32-33	0.0	0.0	0.0	0.009264324909351055	2.2630411992307923E-4
34-35	0.0	0.0	0.0	0.017269833151629985	2.2630411992307923E-4
36-37	0.0	0.0	0.0	0.029023503380134912	2.2630411992307923E-4
38-39	0.0	0.0	0.0	0.05503433316379383	2.2630411992307923E-4
40-41	0.0	0.0	0.0	0.0972400515294481	2.545921349134641E-4
42-43	0.0	0.0	0.0	0.13555616783392443	2.545921349134641E-4
44-45	0.0	0.0	0.0	0.17291049162872774	2.545921349134641E-4
46-47	0.0	0.0	0.0	0.21463531373954547	2.545921349134641E-4
48-49	0.0	0.0	0.0	0.2696979349183297	2.8288014990384905E-4
50-51	0.0	0.0	0.0	0.3375891708952534	2.8288014990384905E-4
52-53	0.0	0.0	0.0	0.4235281604360428	2.8288014990384905E-4
54-55	0.0	2.8288014990384904E-5	0.0	0.5268218471734333	2.8288014990384905E-4
56-57	0.0	2.8288014990384904E-5	0.0	0.6247549550701458	2.970241573990415E-4
58-59	0.0	7.072003747596226E-5	0.0	0.7502123015525028	3.111681648942339E-4
60-61	0.0	1.1315205996153962E-4	0.0	0.8887811429829033	3.111681648942339E-4
62-63	0.0	1.1315205996153962E-4	0.0	1.09064441795429	3.111681648942339E-4
64-65	0.0	1.1315205996153962E-4	0.0	1.265040030370013	3.111681648942339E-4
66-67	0.0	1.1315205996153962E-4	0.0	1.4665214171390293	3.111681648942339E-4
68-69	0.0	1.1315205996153962E-4	0.0	1.6883843187086183	3.3945617988461884E-4
70-71	0.0	1.1315205996153962E-4	0.0	1.925692476462957	3.6774419487500376E-4
72-73	0.0	1.1315205996153962E-4	0.0	2.120823203866632	3.6774419487500376E-4
74-75	0.0	1.1315205996153962E-4	0.0	2.356292640646596	3.6774419487500376E-4
76-77	0.0	1.1315205996153962E-4	0.0	2.655834431379782	3.6774419487500376E-4
78-79	0.0	1.2729606745673206E-4	0.0	2.9920940655704875	3.6774419487500376E-4
80-81	0.0	1.4144007495192452E-4	0.0	3.2989483081786872	3.6774419487500376E-4
82-83	0.0	1.4144007495192452E-4	0.0	3.630611139933455	3.6774419487500376E-4
84-85	0.0	1.4144007495192452E-4	0.0	4.030914840062392	3.6774419487500376E-4
86-87	0.0	1.4144007495192452E-4	0.0	4.4853618008829255	3.818882023701962E-4
88-89	0.0	1.4144007495192452E-4	0.0	4.982028624076609	3.960322098653887E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	6730	0.0	73.358765	2
TATAACG	760	0.0	72.45663	2
ATAACGC	825	0.0	66.75076	3
TAACGCA	855	0.0	66.07531	4
ACCTGGG	7750	0.0	62.11385	3
GTACCTG	8095	0.0	62.046	1
CCTGGGG	6470	0.0	58.407295	4
GTATAAC	1115	0.0	53.371983	1
TATCTAA	1060	0.0	53.29357	2
CTGGGGG	4370	0.0	52.238754	5
TATCACG	395	0.0	51.677925	2
ATCGAGA	3050	0.0	48.251858	3
TACCCGG	995	0.0	47.710136	2
TCGAGAA	3135	0.0	46.94426	4
CCATCGA	3140	0.0	46.698105	1
CGAGAAC	2455	0.0	46.396893	5
CATCGAG	3205	0.0	45.916355	2
AGAACGT	2550	0.0	44.8545	7
CCTAGAG	1600	0.0	44.801308	2
CCCTAGA	1625	0.0	44.53161	1
>>END_MODULE