FastQCFastQC Report
Mon 15 Aug 2016
SRR2920804_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920804_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3080359
Sequences flagged as poor quality0
Sequence length98
%GC50

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CCCTAGAGGTGGAGCCCAGTGACACCATCGAGAACGTGAAGGCCAAGATC106320.3451545745155029No Hit
GGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGGCA95470.30993140734570224No Hit
GGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCAGGCA87300.28340852478558504No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT82260.26704679551961313No Hit
CCATCGAGAACGTGAAGGCCAAGATCCAGGATAAAGAGGGCATCCCCCCT80730.2620798419924431No Hit
CCCTCTCTGATTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTT76730.24909434257500504No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT75960.24659463393714826No Hit
TCTCTATGGTGTCACTGGGCTCGACCTCCAGGGTGATGGTCTTGCCTGTC75210.2441598527963786No Hit
CCCTCTCTGATTACAACATCCAGAAGGAGTCAACCCTGCACCTGGTCCTT61090.19832103985282235No Hit
GGATAAAGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCAGGCA60360.19595118620913993No Hit
GGATAAAGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGGCA54720.17764163203055228No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT52010.168843956175238No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC51040.16569497256650928No Hit
AGGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGGC50610.1642990313791347No Hit
CTGACAGGGTGCGGCCATCTTCCAGCTGCTTGCCTGCAAAGATCAGCCTC45280.14699585340539853No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT44440.14426889852773656No Hit
GTCTGACAGGGTGCGGCCATCTTCCAGCTGCTTGCCTGCAAAGATCAGCC43470.14111991491900783No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT41300.1340752814850477No Hit
AGGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCAGGC40970.13300397778310905No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG37670.12229094076372266No Hit
CTTTATCCTGGATCTTTGCCTTGACATTCTCTATGGTGTCACTGGGCTCG35720.11596050979772164No Hit
CCATCGAGAACGTGAAGGCAAAGATCCAGGACAAGGAGGGCATCCCCCCT35280.11453210486180344No Hit
CCTTGTCCTGGATCTTTGCCTTGACATTCTCTATGGTGTCACTGGGCTCG34080.11063645503657203No Hit
CCCTCTCTGATTACAACATCCAGAAAGAGTCAACCCTGCACCTGGTCCTC33360.10829906514143318No Hit
CCCTAGAGGTGGAGCCCAGTGACACCATAGAGAATGTCAAGGCAAAGATC32480.10544225526959682No Hit
CTCTCAGGCGAAGGACCAGGTGCAGGGTTGACTCCTTCTGGATGTTGTAA31680.10284515538610921No Hit
TCTCATTACAGATGGTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACT31050.10079993922786272No Hit
ATGTCAAGGCAAAGATCCAGGACAAGGAGGGCATCCCCCCTGACCAGCAG31030.10073501173077552No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG51250.070.6948242
TATAACG5450.061.4300352
ATAACGC5800.059.3045043
GTACCTG62650.059.1712571
CCCTAGA30450.058.9811551
ACCTGGG60450.058.9493643
CCTAGAG30550.058.847672
CTAGAGG30900.057.142163
TAACGCA6450.056.1649484
TATCTAA8450.051.5609972
CCTGGGG58400.051.274134
TAGAGGT35250.049.4336244
GTATAAC8600.044.4442561
CTGGGGG44050.043.7221345
ATAAGTA11100.043.3774765
ATATCTA10400.042.9511071
CTATAGC8750.042.455253
TATAAGT6400.042.2736784
TACCCGG9450.041.7369842
GGTCGAC7400.040.284322
Produced by FastQC (version 0.11.5)