##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920804_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	3080359
Sequences flagged as poor quality	0
Sequence length	98
%GC	50
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	29.730606075460685	31.0	30.0	34.0	26.0	34.0
2	29.764195666803772	31.0	30.0	34.0	25.0	34.0
3	29.886003871626652	31.0	30.0	34.0	25.0	34.0
4	33.35167621696043	37.0	35.0	37.0	28.0	37.0
5	33.35340036664557	37.0	35.0	37.0	28.0	37.0
6	33.44448715230919	37.0	35.0	37.0	28.0	37.0
7	33.37325032569256	37.0	35.0	37.0	28.0	37.0
8	33.37503550722497	37.0	35.0	37.0	28.0	37.0
9	34.72847872601862	39.0	35.0	39.0	27.0	39.0
10-11	34.572502425853614	39.0	35.0	39.0	27.0	39.0
12-13	34.39100361353985	38.0	35.0	39.0	26.5	39.0
14-15	35.3287456104954	39.0	35.0	41.0	26.0	41.0
16-17	35.21029334567821	39.0	35.0	41.0	25.0	41.0
18-19	35.0968749097102	39.0	35.0	41.0	25.0	41.0
20-21	35.003855394777034	39.0	35.0	41.0	24.5	41.0
22-23	34.754150246773186	39.0	34.0	40.0	23.5	41.0
24-25	34.55215739464134	39.0	34.0	40.0	23.0	41.0
26-27	34.59998444986445	39.0	34.5	40.0	22.5	41.0
28-29	34.397195261980826	39.0	34.0	40.0	20.0	41.0
30-31	34.13562769794041	38.0	34.0	40.0	18.0	41.0
32-33	33.830802351284376	38.0	33.5	40.0	16.5	41.0
34-35	33.38103919056188	38.0	33.0	40.0	12.0	41.0
36-37	33.05522408264751	38.0	33.0	40.0	8.5	41.0
38-39	32.87425507870998	38.0	33.0	40.0	8.0	41.0
40-41	32.659477190807955	38.0	32.0	40.0	4.5	41.0
42-43	32.44585549931031	38.0	32.0	40.0	2.0	41.0
44-45	32.19134279478463	38.0	31.5	40.0	2.0	41.0
46-47	31.85989295403555	37.0	31.0	40.0	2.0	41.0
48-49	31.66322837695217	37.0	31.0	40.0	2.0	41.0
50-51	30.909299370625305	36.0	30.0	39.0	2.0	40.0
52-53	31.221193536207956	36.5	30.5	39.0	2.0	40.0
54-55	31.596994376304842	37.0	31.0	40.0	2.0	41.0
56-57	31.460965264113696	37.0	31.0	40.0	2.0	41.0
58-59	31.18780570706207	36.0	31.0	40.0	2.0	41.0
60-61	30.721586347565335	36.0	30.0	39.0	2.0	41.0
62-63	30.2109289858747	35.0	29.0	39.0	2.0	40.0
64-65	29.735709863687966	35.0	29.0	38.5	2.0	40.0
66-67	29.329549250590595	35.0	28.0	37.5	2.0	40.0
68-69	28.900060187789798	34.5	28.0	37.0	2.0	39.0
70-71	28.457203851888693	34.0	27.5	36.5	2.0	39.0
72-73	27.992348781424504	34.0	26.0	36.0	2.0	38.5
74-75	27.575324337195763	34.0	26.0	35.0	2.0	37.0
76-77	27.087488179137562	34.0	25.5	35.0	2.0	37.0
78-79	26.691188429660308	33.5	25.0	35.0	2.0	36.0
80-81	26.31302309243825	33.0	23.5	35.0	2.0	36.0
82-83	25.781685024375406	33.0	21.0	35.0	2.0	35.5
84-85	25.404082283915606	33.0	19.5	35.0	2.0	35.0
86-87	25.022217864865752	32.0	17.5	35.0	2.0	35.0
88-89	24.601179927404566	32.0	10.0	35.0	2.0	35.0
90-91	24.09146742311529	32.0	3.5	34.0	2.0	35.0
92-93	23.528607055216618	31.0	2.0	34.0	2.0	35.0
94-95	22.873369305330968	31.0	2.0	34.0	2.0	35.0
96-97	22.299065466070676	31.0	2.0	34.0	2.0	35.0
98	21.912952353930176	30.0	2.0	34.0	2.0	35.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
2	135035.0
3	20775.0
4	16215.0
5	13282.0
6	12760.0
7	12605.0
8	13885.0
9	15611.0
10	17092.0
11	18986.0
12	22476.0
13	26966.0
14	22731.0
15	21200.0
16	23195.0
17	26293.0
18	27930.0
19	25018.0
20	24559.0
21	26313.0
22	28981.0
23	30730.0
24	34418.0
25	38024.0
26	43151.0
27	49454.0
28	57250.0
29	66182.0
30	78850.0
31	95510.0
32	117864.0
33	155133.0
34	225200.0
35	277829.0
36	406886.0
37	547358.0
38	286223.0
39	18384.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	49.24290695280611	14.251019304885778	11.461564285944995	25.044509456363116
2	19.429430024286827	17.332624981043672	36.977613188341934	26.260331806327564
3	20.41149127832579	27.907051519391384	27.8559634374322	23.82549376485062
4	12.37216446470062	14.835140747764614	36.01139166310813	36.78130312442664
5	12.486494508659915	34.18260560912648	27.854225452967768	25.47667442924584
6	28.3367372450719	30.999321382302043	24.110669883336204	16.553271489289852
7	28.542801047758086	32.616455907929804	20.936421610578915	17.904321433733198
8	34.05318850517392	27.860070440094752	20.02792799110668	18.05881306362465
9	33.53644818672109	18.499012615088045	19.121245283423132	28.843293914767727
10-11	29.475963678259582	22.377326798597178	26.824941508441064	21.321768014702183
12-13	27.556357555599526	21.736507924656074	26.89646597493892	23.810668544805477
14-15	25.65446759882998	22.520850309548777	25.400059734635377	26.424622356985868
16-17	25.519202670405466	22.357568175254855	26.14342926592582	25.979799888413858
18-19	24.753607775931357	23.845784550780415	26.60597512918092	24.79463254410731
20-21	25.88958644021314	23.642966078274604	25.388954679779424	25.07849280173283
22-23	25.67501496115462	22.84715768935102	27.3496081014307	24.12821924806366
24-25	23.157197894270002	24.652587853144745	26.66311975222501	25.527094500360242
26-27	23.57327472553181	23.557808296536933	25.57928031391652	27.289636664014736
28-29	24.83978185577232	25.00645680664374	25.18825449206942	24.965506845514525
30-31	27.105370510392063	24.92578489376684	26.054569052203263	21.91427554363783
32-33	26.49823358966148	24.739471525361417	25.274866959000704	23.487427925976398
34-35	25.998270622484647	23.510180056033654	25.693087313682877	24.798462007798822
36-37	25.523648149457344	24.080165609457072	27.118548595531163	23.27763764555442
38-39	27.14801689129866	25.215384294016697	24.412270801279185	23.22432801340546
40-41	26.314643749526766	23.21437429994021	26.192435176747964	24.278546773785063
42-43	24.047043755514316	24.15820682790831	27.242537597667322	24.552211818910052
44-45	24.140675679245597	24.57183401121729	25.595166460708658	25.69232384882845
46-47	24.207422075189303	22.856683505446963	26.004379354712892	26.931515064650842
48-49	23.833216179489252	23.142323662816022	26.72809527411391	26.296364883580814
50-51	23.725651535459086	25.122393438352002	26.16487951894639	24.987075507242526
52-53	25.905431840663574	23.259655764618064	24.992740885161783	25.842171509556582
54-55	23.66387354755386	23.610375791147632	27.61332624380652	25.112424417491987
56-57	24.47000785777589	23.414329203950874	25.866565245145317	26.249097693127922
58-59	25.764298801759487	25.034514616147085	25.383924659570194	23.81726192252323
60-61	26.883923837957447	25.272636871251475	25.28354642291456	22.559892867876524
62-63	28.212770689907646	24.2475114906335	24.80960917985267	22.730108639606183
64-65	26.827060791739527	22.95030762613683	24.97884106964285	25.24379051248079
66-67	25.114979970178382	24.863573200093782	25.859851453171594	24.161595376556242
68-69	24.32159915839261	22.729856354758645	26.45899430395371	26.489550182895034
70-71	23.7310982774659	23.16603720011234	26.681014733445597	26.421849788976164
72-73	22.47691732804373	25.990085577446916	24.959336331116095	26.573660763393264
74-75	24.052067870405523	23.5257186340535	25.502371683838494	26.919841811702476
76-77	23.260034310181048	22.657267626093663	26.815784117355356	27.266913946369932
78-79	22.657886216432317	25.01440858326271	26.532536348981505	25.795168851323464
80-81	25.54906007876051	23.541426508389527	25.41722764706975	25.49228576578021
82-83	25.46293119815905	23.826625195263006	26.480963436009553	24.229480170568397
84-85	24.27001097179041	25.476011794919085	24.75468256267395	25.499294670616557
86-87	24.548991005321785	25.177424056735287	24.44801087723981	25.825574060703122
88-89	23.139586774407956	24.379922020237185	26.42712712605033	26.053364079304526
90-91	24.954269691246882	24.67332874767315	26.43111626278147	23.9412852982985
92-93	25.529007624880023	24.95032176374442	25.117860535908964	24.402810075466594
94-95	25.07091120800431	23.795365042994884	25.08981543122407	26.043908317776733
96-97	23.784185797250124	25.503880555464555	24.729448263036417	25.982485384248903
98	25.76494954218886	23.523491846741223	23.33619584116178	27.375362769908147
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	692.0
1	679.0
2	3020.0
3	4682.5
4	2800.5
5	1668.0
6	1893.5
7	2947.5
8	4398.5
9	4620.5
10	3775.5
11	3194.5
12	3240.5
13	3499.0
14	3866.5
15	4328.0
16	4757.5
17	5078.5
18	5184.5
19	5162.0
20	5208.5
21	5132.5
22	5122.0
23	5404.5
24	5665.0
25	6107.0
26	6870.5
27	7625.5
28	8726.5
29	10426.5
30	12472.0
31	15473.5
32	19418.5
33	23544.5
34	28236.0
35	33083.5
36	36429.0
37	37498.5
38	37880.0
39	37676.0
40	37520.5
41	39425.5
42	43512.5
43	50939.5
44	60613.5
45	72783.5
46	87889.0
47	106065.0
48	126112.0
49	147175.5
50	158524.0
51	160762.5
52	162446.5
53	164897.0
54	168823.5
55	170656.5
56	174069.0
57	180836.0
58	177631.0
59	161279.0
60	120211.5
61	75199.5
62	54192.5
63	37593.5
64	24475.0
65	17292.5
66	12667.0
67	8769.5
68	5973.0
69	4305.5
70	2947.5
71	1851.5
72	1266.0
73	879.0
74	612.0
75	447.5
76	321.5
77	199.0
78	121.0
79	90.5
80	75.5
81	64.0
82	55.0
83	40.0
84	23.0
85	19.0
86	15.0
87	11.5
88	10.0
89	8.0
90	6.5
91	6.5
92	7.0
93	5.0
94	6.0
95	4.5
96	2.5
97	2.0
98	1.5
99	1.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.49604607774613285
2	0.029606938671758714
3	0.0442480892649201
4	0.029671866168845904
5	0.034281718462036405
6	0.0184718729213056
7	0.04557910295520749
8	0.00957680582036055
9	0.0
10-11	0.0
12-13	1.4608686844617787E-4
14-15	0.0022399986495080606
16-17	0.007385502793667881
18-19	0.05619474872896309
20-21	0.09244052397788699
22-23	0.10464689343027875
24-25	0.08331821063713678
26-27	0.0884799466555684
28-29	0.07180981177843232
30-31	0.04775417410762836
32-33	0.040027801954252734
34-35	0.39615512347749077
36-37	0.5834871844483062
38-39	0.5367231546712574
40-41	0.5302953324596256
42-43	0.48833593746702897
44-45	0.46098522931905017
46-47	0.45207393034383325
48-49	0.4274501770735164
50-51	0.4385040834526106
52-53	0.4949747740441942
54-55	0.5111254889446327
56-57	0.47416551122774975
58-59	0.45139219162441785
60-61	0.46223508363797855
62-63	0.4598814618685679
64-65	0.4826872452204435
66-67	0.5130408501087049
68-69	0.44949306233461755
70-71	0.4160067057118992
72-73	0.39613889160321897
74-75	0.3851499127212121
76-77	0.39519744289545466
78-79	0.38552324582946335
80-81	0.3918374449211926
82-83	0.4019499025925225
84-85	0.375930208134831
86-87	0.3874061432449919
88-89	0.4103417815910418
90-91	0.39276266175468505
92-93	0.3932658498571108
94-95	0.39805425276729106
96-97	0.4120461283895806
98	0.3880716500901356
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
98	3080359.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	42.44113680106796
#Duplication Level	Percentage of deduplicated	Percentage of total
1	76.72126667014321	32.56137774298764
2	12.63062106112582	10.721158326753823
3	4.044000263797357	5.148959052581356
4	1.7684930998057329	3.0022743032239942
5	0.987300754524467	2.095108319328526
6	0.6714927354361266	1.7099349027340793
7	0.45479175193088056	1.3511315271787105
8	0.35460904990872205	1.2040008958458248
9	0.2796540036986896	1.0681950445148207
>10	1.799896211839867	14.45149832307718
>50	0.1616797819625704	4.741497457197531
>100	0.10554953410289451	8.73789366684139
>500	0.010670491677408531	3.0382506703672183
>1k	0.008892076397818655	6.854876070968256
>5k	0.0010051912449708046	2.9649385936563704
>10k+	7.732240345929266E-5	0.34890510274330133
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CCCTAGAGGTGGAGCCCAGTGACACCATCGAGAACGTGAAGGCCAAGATC	10632	0.3451545745155029	No Hit
GGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGGCA	9547	0.30993140734570224	No Hit
GGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCAGGCA	8730	0.28340852478558504	No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	8226	0.26704679551961313	No Hit
CCATCGAGAACGTGAAGGCCAAGATCCAGGATAAAGAGGGCATCCCCCCT	8073	0.2620798419924431	No Hit
CCCTCTCTGATTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTT	7673	0.24909434257500504	No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	7596	0.24659463393714826	No Hit
TCTCTATGGTGTCACTGGGCTCGACCTCCAGGGTGATGGTCTTGCCTGTC	7521	0.2441598527963786	No Hit
CCCTCTCTGATTACAACATCCAGAAGGAGTCAACCCTGCACCTGGTCCTT	6109	0.19832103985282235	No Hit
GGATAAAGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCAGGCA	6036	0.19595118620913993	No Hit
GGATAAAGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGGCA	5472	0.17764163203055228	No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	5201	0.168843956175238	No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC	5104	0.16569497256650928	No Hit
AGGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGGC	5061	0.1642990313791347	No Hit
CTGACAGGGTGCGGCCATCTTCCAGCTGCTTGCCTGCAAAGATCAGCCTC	4528	0.14699585340539853	No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4444	0.14426889852773656	No Hit
GTCTGACAGGGTGCGGCCATCTTCCAGCTGCTTGCCTGCAAAGATCAGCC	4347	0.14111991491900783	No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4130	0.1340752814850477	No Hit
AGGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCAGGC	4097	0.13300397778310905	No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG	3767	0.12229094076372266	No Hit
CTTTATCCTGGATCTTTGCCTTGACATTCTCTATGGTGTCACTGGGCTCG	3572	0.11596050979772164	No Hit
CCATCGAGAACGTGAAGGCAAAGATCCAGGACAAGGAGGGCATCCCCCCT	3528	0.11453210486180344	No Hit
CCTTGTCCTGGATCTTTGCCTTGACATTCTCTATGGTGTCACTGGGCTCG	3408	0.11063645503657203	No Hit
CCCTCTCTGATTACAACATCCAGAAAGAGTCAACCCTGCACCTGGTCCTC	3336	0.10829906514143318	No Hit
CCCTAGAGGTGGAGCCCAGTGACACCATAGAGAATGTCAAGGCAAAGATC	3248	0.10544225526959682	No Hit
CTCTCAGGCGAAGGACCAGGTGCAGGGTTGACTCCTTCTGGATGTTGTAA	3168	0.10284515538610921	No Hit
TCTCATTACAGATGGTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACT	3105	0.10079993922786272	No Hit
ATGTCAAGGCAAAGATCCAGGACAAGGAGGGCATCCCCCCTGACCAGCAG	3103	0.10073501173077552	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	3.246374854359508E-5
10-11	0.0	0.0	0.0	3.246374854359508E-5	3.246374854359508E-5
12-13	0.0	0.0	0.0	3.246374854359508E-5	3.246374854359508E-5
14-15	0.0	0.0	0.0	8.11593713589877E-5	3.246374854359508E-5
16-17	0.0	0.0	0.0	1.7855061698977294E-4	3.246374854359508E-5
18-19	0.0	0.0	0.0	2.2724623980516557E-4	3.246374854359508E-5
20-21	0.0	0.0	0.0	3.571012339795459E-4	3.246374854359508E-5
22-23	0.0	0.0	0.0	6.492749708719016E-4	3.246374854359508E-5
24-25	0.0	0.0	0.0	0.00105507182766684	3.246374854359508E-5
26-27	0.0	0.0	0.0	0.0014284049359181835	3.246374854359508E-5
28-29	0.0	0.0	0.0	0.0020614480325182876	3.246374854359508E-5
30-31	0.0	0.0	0.0	0.004285214807754551	3.246374854359508E-5
32-33	0.0	0.0	0.0	0.01029100828831964	3.246374854359508E-5
34-35	0.0	0.0	0.0	0.0197866547373212	3.246374854359508E-5
36-37	0.0	0.0	0.0	0.03160345920718981	3.246374854359508E-5
38-39	0.0	0.0	0.0	0.054815039415860294	3.246374854359508E-5
40-41	0.0	0.0	0.0	0.09388516078807697	3.246374854359508E-5
42-43	0.0	0.0	0.0	0.13248455780641152	3.246374854359508E-5
44-45	0.0	0.0	0.0	0.1738109097024081	3.246374854359508E-5
46-47	0.0	0.0	0.0	0.2184810276983949	3.246374854359508E-5
48-49	0.0	3.246374854359508E-5	0.0	0.2758444713749274	3.246374854359508E-5
50-51	0.0	3.246374854359508E-5	0.0	0.3478328337703495	3.246374854359508E-5
52-53	0.0	3.246374854359508E-5	0.0	0.43899103968076447	6.492749708719016E-5
54-55	0.0	3.246374854359508E-5	0.0	0.5466732935998693	6.492749708719016E-5
56-57	0.0	3.246374854359508E-5	0.0	0.6522291719893687	6.492749708719016E-5
58-59	0.0	3.246374854359508E-5	0.0	0.7871485109365499	6.492749708719016E-5
60-61	0.0	3.246374854359508E-5	0.0	0.9379263910472773	6.492749708719016E-5
62-63	0.0	3.246374854359508E-5	0.0	1.1518949576981123	6.492749708719016E-5
64-65	0.0	3.246374854359508E-5	0.0	1.3397302067713537	6.492749708719016E-5
66-67	0.0	3.246374854359508E-5	0.0	1.558016451978487	6.492749708719016E-5
68-69	0.0	3.246374854359508E-5	0.0	1.794368773250131	6.492749708719016E-5
70-71	0.0	3.246374854359508E-5	0.0	2.042489203368828	6.492749708719016E-5
72-73	0.0	3.246374854359508E-5	0.0	2.244462414932805	6.492749708719016E-5
74-75	0.0	3.246374854359508E-5	0.0	2.493118496902471	6.492749708719016E-5
76-77	0.0	3.246374854359508E-5	0.0	2.8042185991957433	6.492749708719016E-5
78-79	0.0	3.246374854359508E-5	0.0	3.1552653440719087	6.492749708719016E-5
80-81	0.0	3.246374854359508E-5	0.0	3.488473259123368	9.739124563078525E-5
82-83	0.0	3.246374854359508E-5	0.0	3.8556707188999724	9.739124563078525E-5
84-85	0.0	3.246374854359508E-5	0.0	4.289532486310849	9.739124563078525E-5
86	0.0	3.246374854359508E-5	0.0	4.661437189626274	9.739124563078525E-5
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCTGG	5125	0.0	70.694824	2
TATAACG	545	0.0	61.430035	2
ATAACGC	580	0.0	59.304504	3
GTACCTG	6265	0.0	59.171257	1
CCCTAGA	3045	0.0	58.981155	1
ACCTGGG	6045	0.0	58.949364	3
CCTAGAG	3055	0.0	58.84767	2
CTAGAGG	3090	0.0	57.14216	3
TAACGCA	645	0.0	56.164948	4
TATCTAA	845	0.0	51.560997	2
CCTGGGG	5840	0.0	51.27413	4
TAGAGGT	3525	0.0	49.433624	4
GTATAAC	860	0.0	44.444256	1
CTGGGGG	4405	0.0	43.722134	5
ATAAGTA	1110	0.0	43.377476	5
ATATCTA	1040	0.0	42.951107	1
CTATAGC	875	0.0	42.45525	3
TATAAGT	640	0.0	42.273678	4
TACCCGG	945	0.0	41.736984	2
GGTCGAC	740	0.0	40.28432	2
>>END_MODULE