FastQCFastQC Report
Mon 15 Aug 2016
SRR2920804_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920804_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences3080359
Sequences flagged as poor quality0
Sequence length101
%GC50

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
TCTCTATGGTGTCACTGGGCTCGACCTCCAGGGTGATGGTCTTGCCTGTC89960.2920438818981813No Hit
CCCTCTCTGATTACAACATCCAGAAAGAGTCCACCCTGCACCTGGTCCTT87870.28525895845257No Hit
GGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCAGGCA75270.24435463528764018No Hit
GTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70740.2296485571973916No Hit
GTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT70560.2290642097236069No Hit
GGATAAAGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCAGGCA63260.20536567328678246No Hit
CTCTCAGGCGAAGGACCAGGTGCAGGGTTGACTCCTTCTGGATGTTGTAA59480.19309437633730353No Hit
CCATCGAGAACGTGAAGGCCAAGATCCAGGATAAAGAGGGCATCCCCCCT57710.1873482928450872No Hit
CTTTATCCTGGATCTTGGCCTTCACGTTCTCGATGGTGTCACTGGGCTCC56940.1848485842072304No Hit
GGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGGCA56170.18234887556937357No Hit
CTGACAGGGTGCGGCCATCTTCCAGCTGCTTGCCTGCAAAGATCAGCCTC53650.1741680109363876No Hit
GGTATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT50130.16274077144904214No Hit
GTCTGACAGGGTGCGGCCATCTTCCAGCTGCTTGCCTGCAAAGATCAGCC49500.16069555529079566No Hit
GTCTGACAGGGTGCGGCCATCTTCCAGCTGCTTGCCGGCAAAGATCAGCC48780.1583581653956568No Hit
CTGACAGGGTGCGGCCATCTTCCAGCTGCTTGCCGGCAAAGATCAGCCTC48590.1577413541733285No Hit
GTACATGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTGC47630.15462483431314336No Hit
GAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT43810.14222368236949007No Hit
CCATCGAGAACGTGAAGGCAAAGATCCAGGACAAGGAGGGCATCCCCCCT41210.13378310774815533No Hit
CTTTATCCTGGATCTTTGCCTTGACATTCTCTATGGTGTCACTGGGCTCG38480.12492050439575388No Hit
TATCAACGCAGAGTACTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT38090.12365441820255366No Hit
CCTTGTCCTGGATCTTTGCCTTGACATTCTCTATGGTGTCACTGGGCTCG37540.12186891203265593No Hit
GGATAAAGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCCGGCA36990.1200834058627582No Hit
TCACAAAGATCTGCATGCCACCTCTGAGGCGAAGGACCAGGTGCAGGGTG36830.11956398588606068No Hit
TCTCATTACAGATGGTTGTGAGCCACCATGTGGTTGCTGGGATTTGAACT36600.118817319669558No Hit
AGGACAAGGAGGGCATCCCCCCTGACCAGCAGAGGCTGATCTTTGCAGGC36090.11716166849383464No Hit
CCCTAGAGGTGGAGCCCAGTGACACCATAGAGAATGTCAAGGCAAAGATC35710.11592804604917803No Hit
GTACATGGGGGGGCTGGTGAGATGGCTCAGTGGGTAAGAGCACCCGACTG35230.11436978611908546No Hit
AGTCTGACAGGGTGCGGCCATCTTCCAGCTGCTTGCCTGCAAAGATCAGC34650.11248688870355696No Hit
AGTCTGACAGGGTGCGGCCATCTTCCAGCTGCTTGCCGGCAAAGATCAGC33370.10833152888997678No Hit
GGGTTGACTCCTTCTGGATGTTGTAATCAGAGAGGGTGCGGCCATCTTCC32290.10482544404726853No Hit
CTTTATCCTGGATCTTGGCCTTCACATTCTCGATGGTGTCACTGGGCTCC31660.10278022788902204No Hit
GGGTTGACTCCTTCTGGATGTTGTAGTCTGACAGGGTGCGGCCATCTTCC30910.10034544674825241No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TACCTGG51000.075.860712
ACCTGGG59500.064.1531453
GTACCTG62250.063.3203931
TATAACG5000.060.763032
TATCTAA9550.059.6495742
CCTGGGG52900.059.5065774
TACCCGG9750.053.0702822
CTGGGGG39950.052.6357845
CGAGAAC22000.051.998235
TAACGCA6000.051.4362454
CCTAGAG14600.049.747132
ATCGAGA27650.049.6228373
AGAACGT22950.049.432947
TCGAGAA28100.049.338244
CCATCGA27500.049.3360941
CATCGAG27950.049.2544672
GAGAACG23300.048.6911776
CTAAGTA12150.048.053285
CCCTAGA15550.047.4519351
ATATCTA12550.047.4153441
Produced by FastQC (version 0.11.5)