FastQCFastQC Report
Mon 15 Aug 2016
SRR2920803_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920803_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954314
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAACGCCGCCGCATCGCCAGTC22910.24006773451924626No Hit
CTATTGGAGCTGGAATTACCGCGGC22680.23765762631586668No Hit
GTATCTGATCGTCTTCGAACCTCCG22230.23294219722229792No Hit
GAATAGGACCGCGGTTCTATTTTGT20840.21837676068882989No Hit
GTCCTATTCCATTATTCCTAGCTGC20770.21764324949649694No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG20610.2159666524854503No Hit
GAACTACGACGGTATCTGATCGTCT20470.21449963010078443No Hit
CTTTAATATACGCTATTGGAGCTGG19680.2062214323587415No Hit
GTCTTGCGCCGGTCCAAGAATTTCA19160.20077249207283973No Hit
CTCTTAATCATGGCCTCAGTTCCGA18340.19217993239122555No Hit
GATTAAGAGGGACGGCCGGGGGCAT17720.18568311897341963No Hit
GTACATGGGAATAACGCCGCCGCAT17160.17981502943475627No Hit
GTACATGGGTACCTGGTTGATCCTG17160.17981502943475627No Hit
ATACAGGACTCTTTCGAGGCCCTGT16590.17384215258290248No Hit
ATATTAAAGTTGCTGCAGTTAAAAA16360.17143204437952286No Hit
GTCCTGTATTGTTATTTTTCGTCAC16250.1702793839344283No Hit
GCTTTGAACACTCTAATTTTTTCAA15550.16294427201109907No Hit
CCATTATTCCTAGCTGCGGTATCCA15060.15780969366476863No Hit
GAATAATGGAATAGGACCGCGGTTC14910.15623788396691235No Hit
CTCTAGATAACCTCGGGCCGATCGC14600.15298947725800943No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG14110.14785489891167897No Hit
GTACAGTGAAACTGCGAATGGCTCA13810.14471127951596643No Hit
CTTTAAATCCTTTAACGAGGATCCA13500.14146287280706352No Hit
GCGCAAGACGGACCAGAGCGAAAGC13480.1412532981806827No Hit
GTACATGGGGAATAATTGCAATCCC12880.13496605938925763No Hit
GATTTAAAGTGGACTCATTCCAATT12820.13433733551011512No Hit
GCTAAGAGCATCGAGGGGGCGCCGA12770.13381339894416303No Hit
CTTTTTAACTGCAGCAACTTTAATA12360.12951711910335592No Hit
AGCTAATACATGCCGACGGGCGCTG12210.12794530940549964No Hit
AAATAGAACCGCGGTCCTATTCCAT12180.1276309474659284No Hit
CTCCAATAGCGTATATTAAAGTTGC11960.1253256265757392No Hit
GTATTGTTATTTTTCGTCACTACCT11760.12322988031193088No Hit
CTCCCGACCCGGGGAGGTAGTGACG11700.12260115643278836No Hit
ACCTCGGGCCGATCGCACGCCCCCC11670.12228679449321712No Hit
GTATCAACGCAGAGTACATGGGAAT11450.11998147360302792No Hit
TTCTAGAGCTAATACATGCCGACGG11430.1197718989766471No Hit
AGATAACCTCGGGCCGATCGCACGC11410.11956232435026627No Hit
ATCAGATACCGTCGTAGTTCCGACC11180.11715221614688666No Hit
GCATATGCTTGTCTCAAAGATTAAG11170.11704742883369625No Hit
GTTCTATTTTGTTGGTTTTCGGAAC11070.11599955570179207No Hit
CCAATGGATCCTCGTTAAAGGATTT11050.11578998107541123No Hit
GTTAAAAAGCTCGTAGTTGGATCTT10930.11453253331712623No Hit
AAATTAGAGTGTTCAAAGCAGGCCC10690.11201763780055621No Hit
GGTCGGGAGTGGGTAATTTGCGCGC10580.11086497735546162No Hit
GTTTATGGTCGGAACTACGACGGTA10560.1106554027290808No Hit
GTATTAGCTCTAGAATTACCACAGT10510.1101314661631287No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC10230.10719742139379701No Hit
GTATCAACGCAGAGTACATGGGTAC10140.10625433557508324No Hit
GATCAAAACCAACCCGGTGAGCTCC9990.104682525877227No Hit
TATCTAGAGTCACCAAAGCCGCCGG9930.10405380199808448No Hit
ATTCCTAGCTGCGGTATCCAGGCGG9890.10363465274532283No Hit
TAACAATACAGGACTCTTTCGAGGC9780.10248199230022823No Hit
TCCCCGCCCCTTGCCTCTCGGCGCC9710.1017484811078953No Hit
GGGTAGGCACACGCTGAGCCAGTCA9560.10017667141003904No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTCTTA907.2759576E-1216.8888915
GGGTTAG508.7083274E-515.21
TAAGCAG400.00527712814.255
ATCGCCA3850.014.06493416
GCGTTAT1301.8189894E-1213.8846151
CGTTATT1301.8189894E-1213.8846152
TCTCGTC1101.8553692E-1013.81818212
CGCATCG3950.013.70886113
CGCCAGT3900.013.64102618
GCGTTCC707.255525E-613.5714279
GCATCGC4050.013.37037114
TAAGACT500.001498808313.2999994
TTGGACT655.448189E-513.1538464
CGACGCT655.448189E-513.15384615
TGAACAG655.448189E-513.1538465
GTTATTC1450.013.1034493
GCTCGTA2350.012.9361719
CATCGCC4250.012.74117715
CCCCTGG751.4771675E-512.6666671
CTCGTAG2400.012.66666710
Produced by FastQC (version 0.11.5)