##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920803_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	954314
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	30.62575001519416	32.0	32.0	33.0	18.0	34.0
2	30.539046896514144	32.0	32.0	33.0	18.0	34.0
3	30.98094861858885	32.0	32.0	33.0	29.0	34.0
4	31.140073393034157	32.0	32.0	33.0	29.0	34.0
5	31.033989860779577	32.0	32.0	33.0	29.0	34.0
6	33.861524613491994	37.0	33.0	37.0	29.0	37.0
7	33.83309895904283	37.0	33.0	37.0	29.0	37.0
8	33.77533076115409	37.0	33.0	37.0	29.0	37.0
9	33.7866593175831	37.0	33.0	37.0	29.0	37.0
10	33.628561458807056	37.0	33.0	37.0	29.0	37.0
11	33.535644452454854	37.0	33.0	37.0	29.0	37.0
12	33.857153934658825	37.0	33.0	37.0	30.0	37.0
13	35.68426429875282	38.0	37.0	38.0	32.0	38.0
14	35.21859157468087	38.0	36.0	38.0	30.0	38.0
15	35.30796467410098	38.0	36.0	38.0	32.0	38.0
16	35.513766957206954	38.0	37.0	38.0	32.0	38.0
17	35.440278566593385	38.0	37.0	38.0	32.0	38.0
18	35.46852084324447	38.0	37.0	38.0	32.0	38.0
19	35.32688926286317	38.0	36.0	38.0	32.0	38.0
20	35.487380463872476	38.0	37.0	38.0	32.0	38.0
21	35.63170717394904	38.0	37.0	38.0	32.0	38.0
22	35.53038936869835	38.0	37.0	38.0	32.0	38.0
23	35.66696286547195	38.0	36.0	39.0	32.0	39.0
24	35.66099103649323	38.0	36.0	39.0	32.0	39.0
25	35.64287016642321	38.0	36.0	39.0	32.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
13	2.0
14	0.0
15	21.0
16	141.0
17	433.0
18	1016.0
19	2051.0
20	3434.0
21	4958.0
22	6894.0
23	8744.0
24	10553.0
25	12681.0
26	14857.0
27	17444.0
28	19954.0
29	23294.0
30	27219.0
31	32366.0
32	40631.0
33	55419.0
34	88661.0
35	192826.0
36	368361.0
37	22354.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.37500235771454	17.066500124696905	10.981081698476602	26.57741581911195
2	18.07287748057767	19.904140565893407	35.80676800298434	26.21621395054458
3	18.236555263781103	24.69229205481634	29.05720758576318	28.013945095639382
4	13.215880726888635	15.097756084475341	35.655035973484615	36.03132721515141
5	15.216480110320083	35.8367371745568	31.519290296485224	17.42749241863789
6	33.118659057710566	34.793265109806626	17.912762466022713	14.175313366460095
7	30.575051817326372	30.32377184029575	21.03772971998734	18.063446622390533
8	29.046100130564994	30.57871937328804	19.91545759571797	20.459722900429
9	28.575395519713638	15.073340640501973	18.880054154083457	37.471209685700934
10	17.519495679619077	24.59012442445568	28.956926127039946	28.933453768885293
11	37.29007149645871	21.042991136031887	22.14001066735966	19.52692670014974
12	26.151664965619283	22.903048682089963	26.75576382616204	24.18952252612872
13	28.73551053426859	18.438166054359467	25.472538388832188	27.35378502253975
14	24.809968207529177	20.46663886309957	23.493420404604773	31.22997252476648
15	25.844350857632666	25.99052931270977	21.620684198999697	26.544435630657865
16	27.41219039116261	24.89560509227095	22.108771896733657	25.58343261983278
17	25.256808853316905	24.915933937540146	24.352280026699948	25.474977182443006
18	26.296342869118728	23.434978518285654	25.129309441475428	25.13936917112019
19	27.610861132010623	23.732424326890232	23.991147456465566	24.665567084633587
20	26.678556683314085	23.496125964317375	22.924713560277567	26.900603792090973
21	28.555006816450433	23.448589502980692	22.627262238960334	25.369141441608534
22	26.746864226509203	23.14394693548218	23.483144890025255	26.62604394798336
23	24.51373039674905	22.560268928414995	24.654460938105807	28.271539736730144
24	25.697573529719964	24.3858376960059	23.578655947555717	26.33793282671842
25	25.53740849332811	23.250396624967777	23.93927708419347	27.272917797510644
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	42.0
1	42.0
2	55.0
3	68.0
4	68.0
5	68.0
6	285.0
7	502.0
8	502.0
9	502.0
10	658.0
11	814.0
12	814.0
13	814.0
14	1387.5
15	1961.0
16	1961.0
17	1961.0
18	3288.0
19	4615.0
20	4615.0
21	4615.0
22	8289.0
23	11963.0
24	11963.0
25	11963.0
26	18629.0
27	25295.0
28	25295.0
29	25295.0
30	30317.0
31	35339.0
32	35339.0
33	35339.0
34	47842.0
35	60345.0
36	60345.0
37	60345.0
38	65641.0
39	70937.0
40	70937.0
41	70937.0
42	79804.0
43	88671.0
44	88671.0
45	88671.0
46	103691.0
47	118711.0
48	118711.0
49	118711.0
50	120246.0
51	121781.0
52	121781.0
53	121781.0
54	115133.0
55	108485.0
56	108485.0
57	108485.0
58	100411.5
59	92338.0
60	92338.0
61	92338.0
62	86080.0
63	79822.0
64	79822.0
65	79822.0
66	67828.5
67	55835.0
68	55835.0
69	55835.0
70	43105.5
71	30376.0
72	30376.0
73	30376.0
74	24859.0
75	19342.0
76	19342.0
77	19342.0
78	17371.5
79	15401.0
80	15401.0
81	15401.0
82	11139.0
83	6877.0
84	6877.0
85	6877.0
86	5117.5
87	3358.0
88	3358.0
89	3358.0
90	2332.0
91	1306.0
92	1306.0
93	1306.0
94	702.5
95	99.0
96	99.0
97	99.0
98	65.0
99	31.0
100	31.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	1.047873131904174E-4
12	0.0
13	0.0
14	0.0
15	1.047873131904174E-4
16	5.239365659520871E-4
17	5.239365659520871E-4
18	0.0014670223846658438
19	0.001257447758285009
20	0.001257447758285009
21	5.239365659520871E-4
22	4.191492527616696E-4
23	8.382985055233392E-4
24	6.287238791425045E-4
25	0.001257447758285009
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	954314.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.04019505980742
#Duplication Level	Percentage of deduplicated	Percentage of total
1	83.85855369150642	33.577128472412504
2	7.5280555098409145	6.028496220701764
3	2.8171984948576765	3.3840353176889173
4	1.462625674754334	2.342552692665839
5	0.880640742959104	1.7630513562848127
6	0.583811086867415	1.4025545877749683
7	0.41368474904046015	1.1594812631393254
8	0.3123506141185298	1.0005263613085256
9	0.2478844042382696	0.893280590818602
>10	1.4692427226955553	11.415162580307008
>50	0.18322803412312558	5.214912467861269
>100	0.20455180224917321	17.418341599171132
>500	0.025536067976987514	7.035958022340296
>1k	0.012636404772117533	7.364518467525012
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATAACGCCGCCGCATCGCCAGTC	2291	0.24006773451924626	No Hit
CTATTGGAGCTGGAATTACCGCGGC	2268	0.23765762631586668	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2223	0.23294219722229792	No Hit
GAATAGGACCGCGGTTCTATTTTGT	2084	0.21837676068882989	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2077	0.21764324949649694	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2061	0.2159666524854503	No Hit
GAACTACGACGGTATCTGATCGTCT	2047	0.21449963010078443	No Hit
CTTTAATATACGCTATTGGAGCTGG	1968	0.2062214323587415	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1916	0.20077249207283973	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	1834	0.19217993239122555	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1772	0.18568311897341963	No Hit
GTACATGGGAATAACGCCGCCGCAT	1716	0.17981502943475627	No Hit
GTACATGGGTACCTGGTTGATCCTG	1716	0.17981502943475627	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	1659	0.17384215258290248	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	1636	0.17143204437952286	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	1625	0.1702793839344283	No Hit
GCTTTGAACACTCTAATTTTTTCAA	1555	0.16294427201109907	No Hit
CCATTATTCCTAGCTGCGGTATCCA	1506	0.15780969366476863	No Hit
GAATAATGGAATAGGACCGCGGTTC	1491	0.15623788396691235	No Hit
CTCTAGATAACCTCGGGCCGATCGC	1460	0.15298947725800943	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	1411	0.14785489891167897	No Hit
GTACAGTGAAACTGCGAATGGCTCA	1381	0.14471127951596643	No Hit
CTTTAAATCCTTTAACGAGGATCCA	1350	0.14146287280706352	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1348	0.1412532981806827	No Hit
GTACATGGGGAATAATTGCAATCCC	1288	0.13496605938925763	No Hit
GATTTAAAGTGGACTCATTCCAATT	1282	0.13433733551011512	No Hit
GCTAAGAGCATCGAGGGGGCGCCGA	1277	0.13381339894416303	No Hit
CTTTTTAACTGCAGCAACTTTAATA	1236	0.12951711910335592	No Hit
AGCTAATACATGCCGACGGGCGCTG	1221	0.12794530940549964	No Hit
AAATAGAACCGCGGTCCTATTCCAT	1218	0.1276309474659284	No Hit
CTCCAATAGCGTATATTAAAGTTGC	1196	0.1253256265757392	No Hit
GTATTGTTATTTTTCGTCACTACCT	1176	0.12322988031193088	No Hit
CTCCCGACCCGGGGAGGTAGTGACG	1170	0.12260115643278836	No Hit
ACCTCGGGCCGATCGCACGCCCCCC	1167	0.12228679449321712	No Hit
GTATCAACGCAGAGTACATGGGAAT	1145	0.11998147360302792	No Hit
TTCTAGAGCTAATACATGCCGACGG	1143	0.1197718989766471	No Hit
AGATAACCTCGGGCCGATCGCACGC	1141	0.11956232435026627	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1118	0.11715221614688666	No Hit
GCATATGCTTGTCTCAAAGATTAAG	1117	0.11704742883369625	No Hit
GTTCTATTTTGTTGGTTTTCGGAAC	1107	0.11599955570179207	No Hit
CCAATGGATCCTCGTTAAAGGATTT	1105	0.11578998107541123	No Hit
GTTAAAAAGCTCGTAGTTGGATCTT	1093	0.11453253331712623	No Hit
AAATTAGAGTGTTCAAAGCAGGCCC	1069	0.11201763780055621	No Hit
GGTCGGGAGTGGGTAATTTGCGCGC	1058	0.11086497735546162	No Hit
GTTTATGGTCGGAACTACGACGGTA	1056	0.1106554027290808	No Hit
GTATTAGCTCTAGAATTACCACAGT	1051	0.1101314661631287	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1023	0.10719742139379701	No Hit
GTATCAACGCAGAGTACATGGGTAC	1014	0.10625433557508324	No Hit
GATCAAAACCAACCCGGTGAGCTCC	999	0.104682525877227	No Hit
TATCTAGAGTCACCAAAGCCGCCGG	993	0.10405380199808448	No Hit
ATTCCTAGCTGCGGTATCCAGGCGG	989	0.10363465274532283	No Hit
TAACAATACAGGACTCTTTCGAGGC	978	0.10248199230022823	No Hit
TCCCCGCCCCTTGCCTCTCGGCGCC	971	0.1017484811078953	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	956	0.10017667141003904	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.047873131904174E-4
2	0.0	0.0	0.0	0.0	1.047873131904174E-4
3	0.0	0.0	0.0	0.0	1.047873131904174E-4
4	0.0	0.0	0.0	0.0	1.047873131904174E-4
5	0.0	0.0	0.0	0.0	1.047873131904174E-4
6	0.0	0.0	0.0	0.0	1.047873131904174E-4
7	0.0	0.0	0.0	0.0	1.047873131904174E-4
8	0.0	0.0	0.0	0.0	1.047873131904174E-4
9	0.0	0.0	0.0	0.0	1.047873131904174E-4
10	0.0	0.0	0.0	0.0	1.047873131904174E-4
11	0.0	0.0	0.0	0.0	1.047873131904174E-4
12	0.0	0.0	0.0	0.0	1.047873131904174E-4
13	0.0	0.0	0.0	0.0	1.047873131904174E-4
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTCTTA	90	7.2759576E-12	16.88889	15
GGGTTAG	50	8.7083274E-5	15.2	1
TAAGCAG	40	0.005277128	14.25	5
ATCGCCA	385	0.0	14.064934	16
GCGTTAT	130	1.8189894E-12	13.884615	1
CGTTATT	130	1.8189894E-12	13.884615	2
TCTCGTC	110	1.8553692E-10	13.818182	12
CGCATCG	395	0.0	13.708861	13
CGCCAGT	390	0.0	13.641026	18
GCGTTCC	70	7.255525E-6	13.571427	9
GCATCGC	405	0.0	13.370371	14
TAAGACT	50	0.0014988083	13.299999	4
TTGGACT	65	5.448189E-5	13.153846	4
CGACGCT	65	5.448189E-5	13.153846	15
TGAACAG	65	5.448189E-5	13.153846	5
GTTATTC	145	0.0	13.103449	3
GCTCGTA	235	0.0	12.936171	9
CATCGCC	425	0.0	12.741177	15
CCCCTGG	75	1.4771675E-5	12.666667	1
CTCGTAG	240	0.0	12.666667	10
>>END_MODULE