FastQCFastQC Report
Mon 15 Aug 2016
SRR2920803_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920803_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences954314
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAGGACCGCGGTTCTATTTTGT23200.24310656660176838No Hit
CTATTGGAGCTGGAATTACCGCGGC22670.23755283900267626No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG22490.23566666736524874No Hit
GAACTACGACGGTATCTGATCGTCT21530.2256070852989687No Hit
GTATCTGATCGTCTTCGAACCTCCG21440.22466399948025492No Hit
GTCTTGCGCCGGTCCAAGAATTTCA20720.21711931293054487No Hit
CTTTAATATACGCTATTGGAGCTGG20490.2147092047271653No Hit
GTCCTATTCCATTATTCCTAGCTGC20310.21282303308973777No Hit
CTCTTAATCATGGCCTCAGTTCCGA20190.21156558533145275No Hit
GATTAAGAGGGACGGCCGGGGGCAT19410.2033921749026002No Hit
GAATAACGCCGCCGCATCGCCAGTC19220.20140121595198227No Hit
ATACAGGACTCTTTCGAGGCCCTGT18970.19878153312222185No Hit
ATATTAAAGTTGCTGCAGTTAAAAA17870.1872549286712759No Hit
GCTTTGAACACTCTAATTTTTTCAA17580.18421609658875382No Hit
GTACATGGGTACCTGGTTGATCCTG16840.17646183541266292No Hit
CTCTAGATAACCTCGGGCCGATCGC16570.17363257795652165No Hit
CCATTATTCCTAGCTGCGGTATCCA16240.17017459662123788No Hit
GTCCTGTATTGTTATTTTTCGTCAC16080.16849799961019118No Hit
GAATAATGGAATAGGACCGCGGTTC15490.16231554813195656No Hit
GCTAAGAGCATCGAGGGGGCGCCGA15150.15875277948348238No Hit
GCGCAAGACGGACCAGAGCGAAAGC14930.15644745859329318No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG14740.15445649964267524No Hit
CTTTAAATCCTTTAACGAGGATCCA14370.15057936905462982No Hit
GTACATGGGGAATAATTGCAATCCC14050.14722617503253646No Hit
GTACATGGGAATAACGCCGCCGCAT14030.1470166004061556No Hit
AAATAGAACCGCGGTCCTATTCCAT13870.14534000339510894No Hit
GATTTAAAGTGGACTCATTCCAATT13700.14355861907087186No Hit
CTTTTTAACTGCAGCAACTTTAATA13600.14251074593896768No Hit
GTACAGTGAAACTGCGAATGGCTCA13360.13999585042239765No Hit
AGATAACCTCGGGCCGATCGCACGC12980.1360139325211618No Hit
ATCAGATACCGTCGTAGTTCCGACC12630.1323463765594972No Hit
AAATTAGAGTGTTCAAAGCAGGCCC12610.13213680193311636No Hit
GTTCTATTTTGTTGGTTTTCGGAAC12270.12857403328464218No Hit
CTCCAATAGCGTATATTAAAGTTGC12190.12773573477911881No Hit
ACCTCGGGCCGATCGCACGCCCCCC12130.12710701089997634No Hit
GCATATGCTTGTCTCAAAGATTAAG12030.12605913776807215No Hit
CCAATGGATCCTCGTTAAAGGATTT11790.12354424225150212No Hit
GTTAAAAAGCTCGTAGTTGGATCTT11790.12354424225150212No Hit
TATCTAGAGTCACCAAAGCCGCCGG11420.11966711166345669No Hit
GTATTGTTATTTTTCGTCACTACCT11170.11704742883369625No Hit
GGGTAGGCACACGCTGAGCCAGTCA11100.11631391764136331No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC11070.11599955570179207No Hit
CTCCCGACCCGGGGAGGTAGTGACG11050.11578998107541123No Hit
GTATCAACGCAGAGTACATGGGTAC10910.11432295869074541No Hit
GTATTAGCTCTAGAATTACCACAGT10830.11348466018522205No Hit
GATCAAAACCAACCCGGTGAGCTCC10770.11285593630607955No Hit
GTATCAACGCAGAGTACATGGGAAT10680.1119128504873658No Hit
TCCCCGCCCCTTGCCTCTCGGCGCC10570.11076019004227121No Hit
GTTTATGGTCGGAACTACGACGGTA10540.11044582810269994No Hit
GGTCGGGAGTGGGTAATTTGCGCGC10450.1095027422839862No Hit
GTATCAACGCAGAGTACTTTTTTTT10410.10908359303122453No Hit
TAACAATACAGGACTCTTTCGAGGC10320.10814050721251077No Hit
TCGTAGTTCCGACCATAAACGATGC10270.10761657064655868No Hit
ATTCCTAGCTGCGGTATCCAGGCGG10230.10719742139379701No Hit
CTCTAATTTTTTCAAAGTAAACGCT9920.10394901468489406No Hit
CCATGGTAGGCACGGCGACTACCAT9820.10290114155298989No Hit
ATTCGAACGTCTGCCCTATCAACTT9620.10080539528918155No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
ACCTTAT350.00216937516.2857131
TTAAACA508.7083274E-515.24
ACTCGGC456.7589455E-414.77777911
CATCGCC3100.014.40322615
ATCGCCA3050.014.32786916
GTCTTAC400.00527712814.251
GCATCGC3300.014.1060614
CTTACAC551.9589048E-413.8181823
CGCATCG3350.013.61193913
TCGCCAG3300.013.53030317
CGCCAGT3300.013.53030318
CAGTTAA3250.013.15384616
GCGTTCC751.4771675E-512.6666679
TACGCCT1051.9897925E-812.6666675
TAAGACA604.0894543E-412.6666674
CCGCATC3700.012.58108112
AGCCCCG2350.012.53191616
GCTCGTA2600.012.4230779
GCAGTTA3450.012.39130415
GCACCTC853.9388124E-612.2941179
Produced by FastQC (version 0.11.5)