##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920803_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	954314
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.2258051333209	33.0	32.0	33.0	32.0	34.0
2	32.3060439226502	33.0	32.0	34.0	32.0	34.0
3	32.50904733662086	33.0	32.0	34.0	32.0	34.0
4	32.681965265101425	33.0	32.0	34.0	32.0	34.0
5	32.535456883164244	33.0	32.0	34.0	32.0	34.0
6	35.33828278742636	37.0	34.0	37.0	33.0	37.0
7	35.306843449849836	37.0	34.0	37.0	33.0	37.0
8	35.11079686560189	37.0	34.0	37.0	32.0	37.0
9	35.1347407666659	37.0	34.0	37.0	33.0	37.0
10	35.05869032624482	37.0	34.0	37.0	32.0	37.0
11	35.05647826606337	37.0	34.0	37.0	32.0	37.0
12	34.94949356291535	37.0	34.0	37.0	32.0	37.0
13	36.94991166429498	38.0	38.0	38.0	36.0	38.0
14	36.91472513239877	38.0	38.0	38.0	36.0	38.0
15	36.84600351666223	38.0	38.0	38.0	36.0	38.0
16	36.93952409793842	38.0	38.0	38.0	36.0	38.0
17	36.887877574886254	38.0	38.0	38.0	36.0	38.0
18	36.94296636117672	38.0	38.0	38.0	36.0	38.0
19	36.84786244359823	38.0	38.0	38.0	36.0	38.0
20	36.903022485261666	38.0	38.0	38.0	36.0	38.0
21	37.00769243666131	38.0	38.0	38.0	36.0	38.0
22	36.962368780087054	38.0	38.0	38.0	36.0	38.0
23	37.3611568100227	38.0	38.0	39.0	36.0	39.0
24	37.37222444604187	38.0	38.0	39.0	36.0	39.0
25	37.23537850225397	38.0	38.0	39.0	35.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
14	1.0
15	3.0
16	10.0
17	6.0
18	8.0
19	6.0
20	10.0
21	23.0
22	64.0
23	193.0
24	507.0
25	1105.0
26	2336.0
27	4234.0
28	6800.0
29	10543.0
30	15079.0
31	20512.0
32	28243.0
33	41020.0
34	68948.0
35	175311.0
36	513345.0
37	66007.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.29485473334772	17.646812265145435	11.221044645682658	26.837288355824185
2	18.289053707689504	20.187904610013057	35.645709902610676	25.87733177968677
3	18.214759502637495	24.738503259933314	28.98521870160136	28.06151853582783
4	13.565660778318248	15.446069113520288	35.5830470893228	35.40522301883866
5	15.25032641248059	35.77250255157108	31.766483568301418	17.210687467646917
6	33.17052877773982	34.990789195170564	17.654880888261097	14.18380113882852
7	30.335717593999462	30.566773619584332	21.01184725362931	18.085661532786904
8	28.821540918397925	30.758953551975555	19.946369853109143	20.473135676517373
9	28.4205198708182	14.934811812464241	19.087847396140052	37.55682092057751
10	17.474227560320816	24.624075513929377	29.03373522760852	28.867961698141283
11	37.31444786516807	20.962702003743004	22.22937104558877	19.49347908550016
12	26.179747965554313	22.954184890926886	26.75115318438166	24.114913959137137
13	28.77438662746224	18.369949513472505	25.677921522685406	27.17774233637985
14	24.7233090995207	20.274563718021533	23.716407807073985	31.285719375383785
15	25.723294429296857	26.04782073824758	21.748711639984325	26.48017319247124
16	27.144400515136052	24.976239402039795	22.309813048366834	25.56954703445732
17	25.133446643347995	25.066801912158894	24.38180724583313	25.41794419865998
18	26.051173932269673	23.337392095264242	25.58738528408889	25.0240486883772
19	27.332722772588475	23.71818919139822	24.41114769352645	24.537940342486856
20	26.524183864011214	23.526218833633376	23.21667711046888	26.732920191886528
21	28.276542102494567	23.639389131879025	23.044406767583837	25.039661998042572
22	26.626141919745493	23.1257217226196	23.749834959981726	26.498301397653183
23	24.154942712775878	22.493959011394573	25.036413591333673	28.314684684495877
24	25.58319379156127	24.28624121620347	23.916970724520443	26.213594267714818
25	25.393738329312992	23.234595741024442	24.311809320621933	27.05985660904063
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	39.0
1	39.0
2	109.0
3	179.0
4	179.0
5	179.0
6	762.5
7	1346.0
8	1346.0
9	1346.0
10	1255.0
11	1164.0
12	1164.0
13	1164.0
14	1538.0
15	1912.0
16	1912.0
17	1912.0
18	3403.0
19	4894.0
20	4894.0
21	4894.0
22	8640.0
23	12386.0
24	12386.0
25	12386.0
26	19707.5
27	27029.0
28	27029.0
29	27029.0
30	31773.0
31	36517.0
32	36517.0
33	36517.0
34	48999.0
35	61481.0
36	61481.0
37	61481.0
38	65604.5
39	69728.0
40	69728.0
41	69728.0
42	79192.0
43	88656.0
44	88656.0
45	88656.0
46	103683.0
47	118710.0
48	118710.0
49	118710.0
50	120193.0
51	121676.0
52	121676.0
53	121676.0
54	114548.0
55	107420.0
56	107420.0
57	107420.0
58	99515.0
59	91610.0
60	91610.0
61	91610.0
62	85582.0
63	79554.0
64	79554.0
65	79554.0
66	67582.5
67	55611.0
68	55611.0
69	55611.0
70	42099.5
71	28588.0
72	28588.0
73	28588.0
74	23788.5
75	18989.0
76	18989.0
77	18989.0
78	17279.5
79	15570.0
80	15570.0
81	15570.0
82	11134.0
83	6698.0
84	6698.0
85	6698.0
86	4968.5
87	3239.0
88	3239.0
89	3239.0
90	2248.5
91	1258.0
92	1258.0
93	1258.0
94	658.5
95	59.0
96	59.0
97	59.0
98	30.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	3.1436193957125227E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	954314.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.22923440868209
#Duplication Level	Percentage of deduplicated	Percentage of total
1	82.04280443082233	29.723479932699238
2	8.332075476033712	6.037294310641133
3	3.000653572021469	3.261341449200451
4	1.6164870107674454	2.3425634732673433
5	1.0094346768967504	1.828552276477232
6	0.6775185188636891	1.4727586341681416
7	0.4756890325787912	1.2063694626855366
8	0.3539188406752649	1.0257766912378545
9	0.27213116047040076	0.8873193242329948
>10	1.7172856815961004	12.005363649570059
>50	0.2170036052897862	5.540315035995903
>100	0.23667853713613082	18.2313676996728
>500	0.032695201340312854	8.142488867465335
>1k	0.015624255507760127	8.295009192685955
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATAGGACCGCGGTTCTATTTTGT	2320	0.24310656660176838	No Hit
CTATTGGAGCTGGAATTACCGCGGC	2267	0.23755283900267626	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	2249	0.23566666736524874	No Hit
GAACTACGACGGTATCTGATCGTCT	2153	0.2256070852989687	No Hit
GTATCTGATCGTCTTCGAACCTCCG	2144	0.22466399948025492	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	2072	0.21711931293054487	No Hit
CTTTAATATACGCTATTGGAGCTGG	2049	0.2147092047271653	No Hit
GTCCTATTCCATTATTCCTAGCTGC	2031	0.21282303308973777	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	2019	0.21156558533145275	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1941	0.2033921749026002	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1922	0.20140121595198227	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	1897	0.19878153312222185	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	1787	0.1872549286712759	No Hit
GCTTTGAACACTCTAATTTTTTCAA	1758	0.18421609658875382	No Hit
GTACATGGGTACCTGGTTGATCCTG	1684	0.17646183541266292	No Hit
CTCTAGATAACCTCGGGCCGATCGC	1657	0.17363257795652165	No Hit
CCATTATTCCTAGCTGCGGTATCCA	1624	0.17017459662123788	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	1608	0.16849799961019118	No Hit
GAATAATGGAATAGGACCGCGGTTC	1549	0.16231554813195656	No Hit
GCTAAGAGCATCGAGGGGGCGCCGA	1515	0.15875277948348238	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1493	0.15644745859329318	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	1474	0.15445649964267524	No Hit
CTTTAAATCCTTTAACGAGGATCCA	1437	0.15057936905462982	No Hit
GTACATGGGGAATAATTGCAATCCC	1405	0.14722617503253646	No Hit
GTACATGGGAATAACGCCGCCGCAT	1403	0.1470166004061556	No Hit
AAATAGAACCGCGGTCCTATTCCAT	1387	0.14534000339510894	No Hit
GATTTAAAGTGGACTCATTCCAATT	1370	0.14355861907087186	No Hit
CTTTTTAACTGCAGCAACTTTAATA	1360	0.14251074593896768	No Hit
GTACAGTGAAACTGCGAATGGCTCA	1336	0.13999585042239765	No Hit
AGATAACCTCGGGCCGATCGCACGC	1298	0.1360139325211618	No Hit
ATCAGATACCGTCGTAGTTCCGACC	1263	0.1323463765594972	No Hit
AAATTAGAGTGTTCAAAGCAGGCCC	1261	0.13213680193311636	No Hit
GTTCTATTTTGTTGGTTTTCGGAAC	1227	0.12857403328464218	No Hit
CTCCAATAGCGTATATTAAAGTTGC	1219	0.12773573477911881	No Hit
ACCTCGGGCCGATCGCACGCCCCCC	1213	0.12710701089997634	No Hit
GCATATGCTTGTCTCAAAGATTAAG	1203	0.12605913776807215	No Hit
CCAATGGATCCTCGTTAAAGGATTT	1179	0.12354424225150212	No Hit
GTTAAAAAGCTCGTAGTTGGATCTT	1179	0.12354424225150212	No Hit
TATCTAGAGTCACCAAAGCCGCCGG	1142	0.11966711166345669	No Hit
GTATTGTTATTTTTCGTCACTACCT	1117	0.11704742883369625	No Hit
GGGTAGGCACACGCTGAGCCAGTCA	1110	0.11631391764136331	No Hit
GTGTAGCGCGCGTGCAGCCCCGGAC	1107	0.11599955570179207	No Hit
CTCCCGACCCGGGGAGGTAGTGACG	1105	0.11578998107541123	No Hit
GTATCAACGCAGAGTACATGGGTAC	1091	0.11432295869074541	No Hit
GTATTAGCTCTAGAATTACCACAGT	1083	0.11348466018522205	No Hit
GATCAAAACCAACCCGGTGAGCTCC	1077	0.11285593630607955	No Hit
GTATCAACGCAGAGTACATGGGAAT	1068	0.1119128504873658	No Hit
TCCCCGCCCCTTGCCTCTCGGCGCC	1057	0.11076019004227121	No Hit
GTTTATGGTCGGAACTACGACGGTA	1054	0.11044582810269994	No Hit
GGTCGGGAGTGGGTAATTTGCGCGC	1045	0.1095027422839862	No Hit
GTATCAACGCAGAGTACTTTTTTTT	1041	0.10908359303122453	No Hit
TAACAATACAGGACTCTTTCGAGGC	1032	0.10814050721251077	No Hit
TCGTAGTTCCGACCATAAACGATGC	1027	0.10761657064655868	No Hit
ATTCCTAGCTGCGGTATCCAGGCGG	1023	0.10719742139379701	No Hit
CTCTAATTTTTTCAAAGTAAACGCT	992	0.10394901468489406	No Hit
CCATGGTAGGCACGGCGACTACCAT	982	0.10290114155298989	No Hit
ATTCGAACGTCTGCCCTATCAACTT	962	0.10080539528918155	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	1.047873131904174E-4
13	0.0	0.0	0.0	0.0	2.095746263808348E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCTTAT	35	0.002169375	16.285713	1
TTAAACA	50	8.7083274E-5	15.2	4
ACTCGGC	45	6.7589455E-4	14.777779	11
CATCGCC	310	0.0	14.403226	15
ATCGCCA	305	0.0	14.327869	16
GTCTTAC	40	0.005277128	14.25	1
GCATCGC	330	0.0	14.10606	14
CTTACAC	55	1.9589048E-4	13.818182	3
CGCATCG	335	0.0	13.611939	13
TCGCCAG	330	0.0	13.530303	17
CGCCAGT	330	0.0	13.530303	18
CAGTTAA	325	0.0	13.153846	16
GCGTTCC	75	1.4771675E-5	12.666667	9
TACGCCT	105	1.9897925E-8	12.666667	5
TAAGACA	60	4.0894543E-4	12.666667	4
CCGCATC	370	0.0	12.581081	12
AGCCCCG	235	0.0	12.531916	16
GCTCGTA	260	0.0	12.423077	9
GCAGTTA	345	0.0	12.391304	15
GCACCTC	85	3.9388124E-6	12.294117	9
>>END_MODULE