FastQCFastQC Report
Mon 15 Aug 2016
SRR2920802_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920802_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873688
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATAACGCCGCCGCATCGCCAGTC16950.19400518262812355No Hit
GTCCTATTCCATTATTCCTAGCTGC16400.18771002920951185No Hit
GTATCTGATCGTCTTCGAACCTCCG15880.18175824779555172No Hit
GAACTACGACGGTATCTGATCGTCT15070.17248720366995998No Hit
GAATAGGACCGCGGTTCTATTTTGT14960.17122817298623763No Hit
CTATTGGAGCTGGAATTACCGCGGC14830.1697402276327476No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG14380.16458964756297442No Hit
CTTTAATATACGCTATTGGAGCTGG14280.16344507421413593No Hit
CTCTTAATCATGGCCTCAGTTCCGA14180.16230050086529746No Hit
GTACATGGGTACCTGGTTGATCCTG14150.1619571288606459No Hit
GTACATGGGAATAACGCCGCCGCAT13980.16001135416762047No Hit
GTCTTGCGCCGGTCCAAGAATTTCA13700.15680654879087272No Hit
GATTAAGAGGGACGGCCGGGGGCAT12670.1450174432978363No Hit
GTCCTGTATTGTTATTTTTCGTCAC12150.13906566188387615No Hit
ATATTAAAGTTGCTGCAGTTAAAAA12030.13769217386527No Hit
GCTTTGAACACTCTAATTTTTTCAA11940.13666205785131535No Hit
CCATTATTCCTAGCTGCGGTATCCA11830.135403027167593No Hit
ATACAGGACTCTTTCGAGGCCCTGT11740.13437291115363836No Hit
GTACAGTGAAACTGCGAATGGCTCA11040.12636089771176895No Hit
GAATAATGGAATAGGACCGCGGTTC10800.12361392167455659No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG10590.12121031764199576No Hit
CTCTAGATAACCTCGGGCCGATCGC10390.1189211709443188No Hit
GCTAAGAGCATCGAGGGGGCGCCGA10330.11823442693501571No Hit
GCATATGCTTGTCTCAAAGATTAAG10050.11502962155826794No Hit
CTTTAAATCCTTTAACGAGGATCCA9990.11434287754896486No Hit
CTTTTTAACTGCAGCAACTTTAATA9960.11399950554431329No Hit
AAATAGAACCGCGGTCCTATTCCAT9620.11010795615826244No Hit
GATTTAAAGTGGACTCATTCCAATT9530.10907784014430781No Hit
AGCTAATACATGCCGACGGGCGCTG9400.10758989479081778No Hit
GTACATGGGGAATAATTGCAATCCC9070.10381280273965077No Hit
GCGCAAGACGGACCAGAGCGAAAGC8930.10221040005127689No Hit
TTCTAGAGCTAATACATGCCGACGG8790.100607997362903No Hit
CTCCCGACCCGGGGAGGTAGTGACG8760.10026462535825147No Hit

[OK]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GGGTTAG601.4650177E-615.8333341
CCGATAA508.7069806E-515.2000019
AGGCCCG2350.014.95744610
AGAACCG1600.014.843755
TAGAACC1550.014.7096784
GAACCGC1550.014.7096786
TTCTAAT400.00527665614.252
AACCGCG1400.014.257
GTTCTAA400.00527665614.251
GCGTTAT801.2862074E-714.251
CGCCAGT2700.014.07407418
GGTATCA3200.013.9531251
GATAACG551.9586062E-413.81818211
TAACGAA551.9586062E-413.81818213
GTAATAC551.9586062E-413.8181823
TAGATCG551.9586062E-413.8181825
CGATAAC551.9586062E-413.81818210
CTCGTAG1700.013.41176410
GAGTAAT500.001498630713.3000011
TAGGCAT500.001498630713.3000015
Produced by FastQC (version 0.11.5)