FastQCFastQC Report
Mon 15 Aug 2016
SRR2920802_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920802_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences873688
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
GTATCAACGCAGAGTACTTTTTTTT17290.1978967320141744No Hit
GTCCTATTCCATTATTCCTAGCTGC16370.18736665720486032No Hit
CTATTGGAGCTGGAATTACCGCGGC16280.18633654119090567No Hit
GTACTTTTTTTTTTTTTTTTTTTTT16150.18484859583741564No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG15980.18290282114439022No Hit
GTATCTGATCGTCTTCGAACCTCCG15210.17408960635833387No Hit
GAATAGGACCGCGGTTCTATTTTGT15070.17248720366995998No Hit
GAACTACGACGGTATCTGATCGTCT14570.16676433692576756No Hit
GAATAACGCCGCCGCATCGCCAGTC14440.16527639157227753No Hit
GTCTTGCGCCGGTCCAAGAATTTCA14390.16470410489785828No Hit
CTCTTAATCATGGCCTCAGTTCCGA14130.1617282141908782No Hit
CTTTAATATACGCTATTGGAGCTGG13620.15589089011180193No Hit
GATTAAGAGGGACGGCCGGGGGCAT13220.15131259671644798No Hit
GTACATGGGTACCTGGTTGATCCTG13210.15119813938156412No Hit
GCTTTGAACACTCTAATTTTTTCAA12860.14719213266062942No Hit
ATACAGGACTCTTTCGAGGCCCTGT12460.14261383926527546No Hit
ATATTAAAGTTGCTGCAGTTAAAAA12000.13734880186061843No Hit
GTCCTGTATTGTTATTTTTCGTCAC11780.13483074049317376No Hit
GCTAAGAGCATCGAGGGGGCGCCGA11560.1323126791257291No Hit
CCATTATTCCTAGCTGCGGTATCCA11540.13208376445596137No Hit
CTCTAGATAACCTCGGGCCGATCGC11270.12899341641409748No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG11230.12853558707456209No Hit
GTACATGGGAATAACGCCGCCGCAT10900.12475849502339507No Hit
TATCAACGCAGAGTACTTTTTTTTT10690.12235489099083426No Hit
GATTTAAAGTGGACTCATTCCAATT10570.12098140297222806No Hit
CTTTAAATCCTTTAACGAGGATCCA10550.12075248830246037No Hit
GTACAGTGAAACTGCGAATGGCTCA10500.12018020162804113No Hit
GTACATGGGGAATAATTGCAATCCC10470.1198368296233896No Hit
AGATAACCTCGGGCCGATCGCACGC10340.11834888426989956No Hit
GAATAATGGAATAGGACCGCGGTTC10280.11766214026059645No Hit
GCGCAAGACGGACCAGAGCGAAAGC10060.11514407889315179No Hit
GCATATGCTTGTCTCAAAGATTAAG9720.11125252950710093No Hit
CTTTTTAACTGCAGCAACTTTAATA9570.1095356694838432No Hit
AAATAGAACCGCGGTCCTATTCCAT9320.10667423611174698No Hit
CTCCAATAGCGTATATTAAAGTTGC9260.10598749210244389No Hit
ACCTCGGGCCGATCGCACGCCCCCC9160.1048429187536054No Hit
GATCAAAACCAACCCGGTGAGCTCC8760.10026462535825147No Hit

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AATCGTA359.9333E-519.013
GTGTTAG453.5360266E-516.888891
GCGGTGT951.8189894E-1116.09
CGTAAAT456.758139E-414.77777816
CGTTATT1006.5847416E-1014.2500012
TGTTAGG602.565722E-514.2500012
GTCTTAG602.565722E-514.2500011
GGTATCA3750.014.18666651
GCGTTAT954.8894435E-914.01
TTTATAC551.9586062E-413.8181823
AGAATTT2700.013.72222117
GTTTAAA852.6944508E-713.4117641
TTAGGGG500.001498630713.3000014
AACCGCG2000.013.3000017
GTTAGGG500.001498630713.3000013
ATATAAC500.001498630713.3000013
TCTCGTC1001.00480975E-813.30000112
TATAGGA500.001498630713.3000012
TAGCACT655.44716E-513.1538464
CGGTGTG1301.4551915E-1113.15384610
Produced by FastQC (version 0.11.5)