##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920802_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	873688
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.431303852176065	33.0	32.0	34.0	32.0	34.0
2	32.555413374110664	33.0	32.0	34.0	32.0	34.0
3	32.756598465356056	33.0	32.0	34.0	32.0	34.0
4	32.91562205272363	33.0	32.0	34.0	32.0	34.0
5	32.69337108899287	33.0	32.0	34.0	32.0	34.0
6	35.41774638085907	37.0	34.0	37.0	33.0	37.0
7	35.392713417146624	37.0	34.0	37.0	33.0	37.0
8	35.196569026929524	37.0	34.0	37.0	33.0	37.0
9	35.25972086145168	37.0	34.0	37.0	33.0	37.0
10	35.20472754576004	37.0	34.0	37.0	33.0	37.0
11	35.167073371729956	37.0	34.0	37.0	33.0	37.0
12	35.08812299127377	37.0	34.0	37.0	33.0	37.0
13	37.051609956872475	38.0	38.0	38.0	36.0	38.0
14	37.01152699819615	38.0	38.0	38.0	36.0	38.0
15	36.94711613299027	38.0	38.0	38.0	36.0	38.0
16	37.04324198111912	38.0	38.0	38.0	36.0	38.0
17	36.994518638232414	38.0	38.0	38.0	36.0	38.0
18	37.04851960883061	38.0	38.0	38.0	36.0	38.0
19	36.964078710020054	38.0	38.0	38.0	36.0	38.0
20	37.01507975387095	38.0	38.0	38.0	36.0	38.0
21	37.10433472818672	38.0	38.0	38.0	36.0	38.0
22	37.06875223191803	38.0	38.0	38.0	36.0	38.0
23	37.504796906904986	39.0	38.0	39.0	36.0	39.0
24	37.506207021270754	39.0	38.0	39.0	36.0	39.0
25	37.38176786221168	38.0	38.0	39.0	36.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	3.0
16	2.0
17	6.0
18	8.0
19	6.0
20	9.0
21	18.0
22	61.0
23	156.0
24	391.0
25	870.0
26	1749.0
27	3149.0
28	5183.0
29	8298.0
30	11754.0
31	16282.0
32	23053.0
33	33314.0
34	56747.0
35	149669.0
36	491132.0
37	71828.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.96341943577112	17.165395427200554	11.152493796412449	26.718691340615873
2	18.039048264368972	20.242924247557482	35.75326661233758	25.96476087573596
3	18.48566078508575	24.605580023990257	29.344457060186247	27.564302130737744
4	13.510085980349965	15.508968876761497	35.707140306379394	35.27380483650914
5	14.883001712281729	35.76723040719341	32.15404125958008	17.195726620944775
6	32.801869775022666	34.89643900339709	17.9595004166247	14.342190804955546
7	29.87634029539149	30.626608125555116	21.19578156046552	18.30127001858787
8	28.696399630073895	30.864564924778637	20.103629671003837	20.33540577414363
9	28.142769501240718	14.971019402807409	19.126850775105073	37.7593603208468
10	17.31682248125189	24.938650868502258	29.76348536319601	27.98104128704984
11	36.6963950517805	21.200703225865528	22.64732948146249	19.455572240891485
12	25.734243803279888	23.018400161155927	27.44045929439342	23.80689674117076
13	28.56958090302259	18.890839750574575	25.888990120042855	26.650589226359983
14	24.275370612850352	20.16795469320856	24.290593438389905	31.266081255551182
15	25.40268997936332	25.989513406975266	22.16423043950522	26.443566174156192
16	26.61963222484966	25.054767960113818	22.841386951376126	25.4842128636604
17	24.956849584748788	24.97184349561857	24.696001318548497	25.375305601084143
18	25.55225664081457	23.676758751407824	25.93797785937314	24.833006748404465
19	26.834751078188095	23.77919806612887	24.959367646116235	24.426683209566804
20	26.11676021646171	23.858745913873143	23.79945701440331	26.225036855261834
21	27.534657681002827	23.72952358278928	23.826011116096364	24.909807620111525
22	26.128205949950097	23.277646024667845	24.339123348380657	26.255024677001398
23	24.270220032780582	22.70204008753697	25.503268901484283	27.524470978198167
24	25.269890395656113	24.327219785552735	24.529122524287846	25.873767294503303
25	25.190342547911843	23.374476929979583	24.893669135892903	26.54151138621567
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	92.0
1	92.0
2	167.5
3	243.0
4	243.0
5	243.0
6	1247.0
7	2251.0
8	2251.0
9	2251.0
10	1937.5
11	1624.0
12	1624.0
13	1624.0
14	1815.0
15	2006.0
16	2006.0
17	2006.0
18	3449.5
19	4893.0
20	4893.0
21	4893.0
22	8303.5
23	11714.0
24	11714.0
25	11714.0
26	18064.0
27	24414.0
28	24414.0
29	24414.0
30	29502.0
31	34590.0
32	34590.0
33	34590.0
34	44806.0
35	55022.0
36	55022.0
37	55022.0
38	60166.5
39	65311.0
40	65311.0
41	65311.0
42	73732.5
43	82154.0
44	82154.0
45	82154.0
46	95514.0
47	108874.0
48	108874.0
49	108874.0
50	111831.5
51	114789.0
52	114789.0
53	114789.0
54	109133.5
55	103478.0
56	103478.0
57	103478.0
58	94129.0
59	84780.0
60	84780.0
61	84780.0
62	77693.5
63	70607.0
64	70607.0
65	70607.0
66	59244.0
67	47881.0
68	47881.0
69	47881.0
70	35991.0
71	24101.0
72	24101.0
73	24101.0
74	19506.0
75	14911.0
76	14911.0
77	14911.0
78	13232.5
79	11554.0
80	11554.0
81	11554.0
82	8272.0
83	4990.0
84	4990.0
85	4990.0
86	3663.5
87	2337.0
88	2337.0
89	2337.0
90	1677.0
91	1017.0
92	1017.0
93	1017.0
94	536.0
95	55.0
96	55.0
97	55.0
98	27.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	1.144573348838487E-4
16	2.289146697676974E-4
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	873688.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.47411047623451
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.69802840711489	30.66310749673415
2	9.037886491831687	6.954492867167997
3	3.503252040039289	4.043535180436966
4	1.8560621975272376	2.8564136815370214
5	1.1517323970227145	2.215593974105515
6	0.8225843301348991	1.8988920236177682
7	0.5840892920293708	1.5730621164666527
8	0.43278343561787574	1.332076617139718
9	0.3196801716432087	1.106946921677618
>10	2.137924366790176	15.512721409309323
>50	0.2247102097079821	6.010818287806465
>100	0.19997413559638919	15.587968661308626
>500	0.022053779484542423	5.669467590004013
>1k	0.009238745459740745	4.574903172688241
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GTATCAACGCAGAGTACTTTTTTTT	1729	0.1978967320141744	No Hit
GTCCTATTCCATTATTCCTAGCTGC	1637	0.18736665720486032	No Hit
CTATTGGAGCTGGAATTACCGCGGC	1628	0.18633654119090567	No Hit
GTACTTTTTTTTTTTTTTTTTTTTT	1615	0.18484859583741564	No Hit
GTTCAAAGCAGGCCCGAGCCGCCTG	1598	0.18290282114439022	No Hit
GTATCTGATCGTCTTCGAACCTCCG	1521	0.17408960635833387	No Hit
GAATAGGACCGCGGTTCTATTTTGT	1507	0.17248720366995998	No Hit
GAACTACGACGGTATCTGATCGTCT	1457	0.16676433692576756	No Hit
GAATAACGCCGCCGCATCGCCAGTC	1444	0.16527639157227753	No Hit
GTCTTGCGCCGGTCCAAGAATTTCA	1439	0.16470410489785828	No Hit
CTCTTAATCATGGCCTCAGTTCCGA	1413	0.1617282141908782	No Hit
CTTTAATATACGCTATTGGAGCTGG	1362	0.15589089011180193	No Hit
GATTAAGAGGGACGGCCGGGGGCAT	1322	0.15131259671644798	No Hit
GTACATGGGTACCTGGTTGATCCTG	1321	0.15119813938156412	No Hit
GCTTTGAACACTCTAATTTTTTCAA	1286	0.14719213266062942	No Hit
ATACAGGACTCTTTCGAGGCCCTGT	1246	0.14261383926527546	No Hit
ATATTAAAGTTGCTGCAGTTAAAAA	1200	0.13734880186061843	No Hit
GTCCTGTATTGTTATTTTTCGTCAC	1178	0.13483074049317376	No Hit
GCTAAGAGCATCGAGGGGGCGCCGA	1156	0.1323126791257291	No Hit
CCATTATTCCTAGCTGCGGTATCCA	1154	0.13208376445596137	No Hit
CTCTAGATAACCTCGGGCCGATCGC	1127	0.12899341641409748	No Hit
GCTCTTAGCTGAGTGTCCCGCGGGG	1123	0.12853558707456209	No Hit
GTACATGGGAATAACGCCGCCGCAT	1090	0.12475849502339507	No Hit
TATCAACGCAGAGTACTTTTTTTTT	1069	0.12235489099083426	No Hit
GATTTAAAGTGGACTCATTCCAATT	1057	0.12098140297222806	No Hit
CTTTAAATCCTTTAACGAGGATCCA	1055	0.12075248830246037	No Hit
GTACAGTGAAACTGCGAATGGCTCA	1050	0.12018020162804113	No Hit
GTACATGGGGAATAATTGCAATCCC	1047	0.1198368296233896	No Hit
AGATAACCTCGGGCCGATCGCACGC	1034	0.11834888426989956	No Hit
GAATAATGGAATAGGACCGCGGTTC	1028	0.11766214026059645	No Hit
GCGCAAGACGGACCAGAGCGAAAGC	1006	0.11514407889315179	No Hit
GCATATGCTTGTCTCAAAGATTAAG	972	0.11125252950710093	No Hit
CTTTTTAACTGCAGCAACTTTAATA	957	0.1095356694838432	No Hit
AAATAGAACCGCGGTCCTATTCCAT	932	0.10667423611174698	No Hit
CTCCAATAGCGTATATTAAAGTTGC	926	0.10598749210244389	No Hit
ACCTCGGGCCGATCGCACGCCCCCC	916	0.1048429187536054	No Hit
GATCAAAACCAACCCGGTGAGCTCC	876	0.10026462535825147	No Hit
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	0.0
13	0.0	0.0	0.0	1.144573348838487E-4	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AATCGTA	35	9.9333E-5	19.0	13
GTGTTAG	45	3.5360266E-5	16.88889	1
GCGGTGT	95	1.8189894E-11	16.0	9
CGTAAAT	45	6.758139E-4	14.777778	16
CGTTATT	100	6.5847416E-10	14.250001	2
TGTTAGG	60	2.565722E-5	14.250001	2
GTCTTAG	60	2.565722E-5	14.250001	1
GGTATCA	375	0.0	14.1866665	1
GCGTTAT	95	4.8894435E-9	14.0	1
TTTATAC	55	1.9586062E-4	13.818182	3
AGAATTT	270	0.0	13.722221	17
GTTTAAA	85	2.6944508E-7	13.411764	1
TTAGGGG	50	0.0014986307	13.300001	4
AACCGCG	200	0.0	13.300001	7
GTTAGGG	50	0.0014986307	13.300001	3
ATATAAC	50	0.0014986307	13.300001	3
TCTCGTC	100	1.00480975E-8	13.300001	12
TATAGGA	50	0.0014986307	13.300001	2
TAGCACT	65	5.44716E-5	13.153846	4
CGGTGTG	130	1.4551915E-11	13.153846	10
>>END_MODULE