FastQCFastQC Report
Mon 15 Aug 2016
SRR2920801_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920801_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences839796
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CCTAAAC453.5357632E-516.888893
ACGCTCT350.00216908316.2857159
TAATACT653.367666E-614.6153844
TAGGGAC400.005276429514.254
TTAGACA500.001498546713.2999994
GTTAGGC500.001498546713.2999993
TCTTATA655.4466684E-513.1538462
GTAATAC604.0885454E-412.6666673
GGGTTAG701.08974964E-412.2142861
GCCTTAT550.003066427812.0909091
TTAGGCA752.0716511E-411.4000014
GTATAAG1501.7644197E-1011.4000011
TAGCACA855.3158758E-511.1764714
ATTAGCC700.001490166310.8571433
GTCCTTA700.001490166310.8571431
CTTATAC700.001490166310.8571433
GTATTAA1106.0333623E-610.3636361
ATCACTG1201.5200876E-610.2916675
CTTGTAC1409.6575604E-810.1785723
GTTCTAG750.00264540410.1333341
Produced by FastQC (version 0.11.5)