##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920801_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	839796
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.16373738384084	33.0	32.0	34.0	32.0	34.0
2	32.34839056151732	33.0	32.0	34.0	32.0	34.0
3	32.54712453976918	33.0	32.0	34.0	32.0	34.0
4	32.72096914012451	33.0	32.0	34.0	32.0	34.0
5	32.68888634858942	33.0	32.0	34.0	32.0	34.0
6	35.37338829906311	37.0	35.0	37.0	33.0	37.0
7	35.49940938037333	37.0	35.0	37.0	33.0	37.0
8	35.48461769286827	37.0	35.0	37.0	33.0	37.0
9	35.48907353690658	37.0	34.0	37.0	33.0	37.0
10	35.47558335595787	37.0	35.0	37.0	33.0	37.0
11	35.407433471938425	37.0	34.0	37.0	33.0	37.0
12	35.339443150479404	37.0	34.0	37.0	33.0	37.0
13	37.09224263987921	38.0	38.0	38.0	37.0	38.0
14	37.1402078600041	38.0	38.0	38.0	37.0	38.0
15	37.09484208069579	38.0	38.0	38.0	37.0	38.0
16	37.075973212542095	38.0	38.0	38.0	37.0	38.0
17	37.07486103768058	38.0	38.0	38.0	37.0	38.0
18	37.05500145273376	38.0	38.0	38.0	37.0	38.0
19	37.07528733168532	38.0	38.0	38.0	37.0	38.0
20	37.10540059728791	38.0	38.0	38.0	37.0	38.0
21	37.11357043853507	38.0	38.0	38.0	37.0	38.0
22	36.995375067278246	38.0	38.0	38.0	37.0	38.0
23	37.64171655973593	39.0	38.0	39.0	37.0	39.0
24	37.68446146445089	39.0	38.0	39.0	37.0	39.0
25	37.497506537301916	39.0	38.0	39.0	36.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
15	1.0
16	3.0
17	6.0
18	54.0
19	148.0
20	388.0
21	835.0
22	1425.0
23	2138.0
24	2889.0
25	3724.0
26	4514.0
27	5401.0
28	6425.0
29	7536.0
30	9163.0
31	11084.0
32	14087.0
33	19734.0
34	34150.0
35	99165.0
36	509075.0
37	107851.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.473181582193774	17.02865934107807	11.926944162629972	26.571214914098185
2	17.054022643594397	19.585708910259157	37.12818351123368	26.232084934912763
3	19.569395424603115	22.426517868625236	28.68672868172747	29.317358025044175
4	14.134742246926635	15.496263378249003	35.415386593887085	34.953607780937276
5	15.379925600979286	35.142225016551635	34.180562898608706	15.297286483860367
6	34.25439035194262	34.29058961938375	17.064739531981576	14.390280496692053
7	29.7737783938004	29.640769901261738	21.40591286455282	19.179538840385046
8	26.67326350685166	33.97884724385446	20.064396591553187	19.283492657740688
9	26.162782390009003	15.947087149736364	18.891611772382817	38.998518687871815
10	15.620341130465018	26.82746762308942	32.86345731582432	24.688733930621247
11	35.75404026692197	21.664428027759122	23.524284469085348	19.05724723623356
12	24.259582088983514	22.865672139424337	29.546580359992188	23.32816541159996
13	28.89380278067531	20.259443960199857	25.47511538516497	25.37163787395987
14	23.052384150436534	19.494258129355224	25.552753287703204	31.900604432505038
15	24.648724213975775	27.293533191394097	22.318991755140534	25.738750839489587
16	25.01428918451624	26.299720408289634	24.044887091627015	24.641103315567115
17	24.28006325345679	26.169569752654215	25.470590476734827	24.079776517154166
18	24.302925948682773	26.133132332137805	25.739703451790675	23.824238267388747
19	25.026434991355046	25.71862690462922	25.502026682670554	23.752911421345182
20	24.931054684709146	25.66147016656426	25.23529523836741	24.172179910359183
21	24.50095023077033	25.43939242387437	25.472257548261723	24.58739979709358
22	24.716240610815007	25.124792211441825	25.205168874345674	24.95379830339749
23	24.48439859203902	25.125744823742906	25.481545518197272	24.908311066020797
24	24.346627037994942	25.61860261301554	25.365445894002832	24.66932445498669
25	24.780899170750992	25.06275333533382	25.306145778260436	24.850201715654755
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	4.0
2	10.5
3	17.0
4	17.0
5	17.0
6	77.0
7	137.0
8	137.0
9	137.0
10	352.5
11	568.0
12	568.0
13	568.0
14	1220.0
15	1872.0
16	1872.0
17	1872.0
18	3512.0
19	5152.0
20	5152.0
21	5152.0
22	8061.5
23	10971.0
24	10971.0
25	10971.0
26	15921.0
27	20871.0
28	20871.0
29	20871.0
30	27586.0
31	34301.0
32	34301.0
33	34301.0
34	43470.5
35	52640.0
36	52640.0
37	52640.0
38	64734.0
39	76828.0
40	76828.0
41	76828.0
42	87113.0
43	97398.0
44	97398.0
45	97398.0
46	105151.5
47	112905.0
48	112905.0
49	112905.0
50	114376.5
51	115848.0
52	115848.0
53	115848.0
54	112029.5
55	108211.0
56	108211.0
57	108211.0
58	96697.5
59	85184.0
60	85184.0
61	85184.0
62	72036.0
63	58888.0
64	58888.0
65	58888.0
66	46054.5
67	33221.0
68	33221.0
69	33221.0
70	24569.0
71	15917.0
72	15917.0
73	15917.0
74	11054.5
75	6192.0
76	6192.0
77	6192.0
78	4049.5
79	1907.0
80	1907.0
81	1907.0
82	1256.0
83	605.0
84	605.0
85	605.0
86	368.0
87	131.0
88	131.0
89	131.0
90	78.0
91	25.0
92	25.0
93	25.0
94	14.0
95	3.0
96	3.0
97	3.0
98	1.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	839796.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.97884014241954
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.75504827113919	73.49238546066148
2	5.699995949675584	9.23158121642436
3	1.499249629883401	3.6422248833572883
4	0.6914645566058107	2.2397599117412383
5	0.3833504323202576	1.5521636688694782
6	0.2302748421257043	1.1188433777590983
7	0.15234711808699342	0.8635825045207466
8	0.10491697970001146	0.6796844261882163
9	0.08159223086283605	0.5946519797914491
>10	0.38420958580518993	5.458815070074507
>50	0.014558879870901667	0.8005618155486086
>100	0.002991523924155502	0.32574568506336227
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	0.0	2.3815307527066097E-4
13	0.0	1.1907653763533048E-4	0.0	0.0	2.3815307527066097E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CCTAAAC	45	3.5357632E-5	16.88889	3
ACGCTCT	35	0.002169083	16.285715	9
TAATACT	65	3.367666E-6	14.615384	4
TAGGGAC	40	0.0052764295	14.25	4
TTAGACA	50	0.0014985467	13.299999	4
GTTAGGC	50	0.0014985467	13.299999	3
TCTTATA	65	5.4466684E-5	13.153846	2
GTAATAC	60	4.0885454E-4	12.666667	3
GGGTTAG	70	1.08974964E-4	12.214286	1
GCCTTAT	55	0.0030664278	12.090909	1
TTAGGCA	75	2.0716511E-4	11.400001	4
GTATAAG	150	1.7644197E-10	11.400001	1
TAGCACA	85	5.3158758E-5	11.176471	4
ATTAGCC	70	0.0014901663	10.857143	3
GTCCTTA	70	0.0014901663	10.857143	1
CTTATAC	70	0.0014901663	10.857143	3
GTATTAA	110	6.0333623E-6	10.363636	1
ATCACTG	120	1.5200876E-6	10.291667	5
CTTGTAC	140	9.6575604E-8	10.178572	3
GTTCTAG	75	0.002645404	10.133334	1
>>END_MODULE