FastQCFastQC Report
Mon 15 Aug 2016
SRR2920801_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920801_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences839796
Sequences flagged as poor quality0
Sequence length25
%GC49

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[OK]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
AAGTGCG350.00216908316.2857157
AGAACCG551.1270771E-515.5454555
GTATAAA602.5654876E-514.2500011
GGGTAAC551.9584646E-413.8181821
TACTTAT604.0885454E-412.6666677
GCCTTAG1001.4393481E-712.3499991
AGCCGTG550.003066427812.0909097
TAACACA1103.8040525E-812.0909094
TAGGGTG802.8626859E-511.8755
CTTAGAC658.016685E-411.6923073
GACTGTC1552.5465852E-1111.6451627
GGAGCGG752.0716511E-411.4000016
ATAACAC855.3158758E-511.1764713
CAATACA855.3158758E-511.1764714
TCAGGAC1201.2719102E-711.0833343
ATCTTAC600.00587184711.0833341
ATAAGGC600.00587184711.0833343
ACGCAGA1053.4594195E-610.8571431
ATGGAAC803.7667857E-410.68753
ACAGATC1002.394772E-510.458
Produced by FastQC (version 0.11.5)