##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920801_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	839796
Sequences flagged as poor quality	0
Sequence length	25
%GC	49
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.12850739941605	33.0	33.0	34.0	32.0	34.0
2	33.29993712758813	34.0	33.0	34.0	32.0	35.0
3	33.46255519197519	34.0	33.0	34.0	32.0	35.0
4	33.532626971312084	34.0	33.0	34.0	32.0	35.0
5	33.44236338348837	34.0	33.0	34.0	32.0	35.0
6	36.04425241368142	37.0	37.0	37.0	33.0	37.0
7	36.09281658879061	37.0	37.0	37.0	34.0	37.0
8	36.04687090674402	37.0	37.0	37.0	34.0	37.0
9	36.01315676664333	37.0	36.0	37.0	34.0	37.0
10	35.97636807034089	37.0	36.0	37.0	33.0	37.0
11	35.88975536916108	37.0	35.0	37.0	33.0	37.0
12	35.89264654749487	37.0	35.0	37.0	33.0	37.0
13	37.592892797774695	38.0	38.0	38.0	38.0	38.0
14	37.59094589638436	38.0	38.0	38.0	38.0	38.0
15	37.58081486456235	38.0	38.0	38.0	38.0	38.0
16	37.57062905753302	38.0	38.0	38.0	38.0	38.0
17	37.58978132784629	38.0	38.0	38.0	38.0	38.0
18	37.620543560578994	38.0	38.0	38.0	38.0	38.0
19	37.62504941676312	38.0	38.0	38.0	38.0	38.0
20	37.578972750525125	38.0	38.0	38.0	38.0	38.0
21	37.549200043820164	38.0	38.0	38.0	38.0	38.0
22	37.5920568804805	38.0	38.0	38.0	38.0	38.0
23	38.34217119395663	39.0	38.0	39.0	38.0	39.0
24	38.288555792120945	39.0	38.0	39.0	38.0	39.0
25	38.25169326836517	39.0	38.0	39.0	38.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
20	1.0
21	2.0
22	9.0
23	28.0
24	69.0
25	215.0
26	417.0
27	845.0
28	1485.0
29	2565.0
30	3934.0
31	5777.0
32	8451.0
33	12990.0
34	23458.0
35	71259.0
36	519017.0
37	189274.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.542960433248076	17.170360420864114	11.977313538049717	26.309365607838092
2	17.350880451919277	19.711334657464434	36.819894355295816	26.11789053532048
3	19.703594682518137	22.50534653653983	28.60885262611396	29.182206154828076
4	14.253461554949059	15.548299825195643	35.3564437077576	34.841794912097704
5	15.439344793259316	35.35965877427375	34.00778284249984	15.193213589967087
6	34.5320768377082	34.20735511957666	17.011988625809124	14.24857941690601
7	29.942390771092025	29.765681189241196	21.312795012121992	18.979133027544783
8	26.73244454605642	34.02195295047845	20.014741675359254	19.23086082810587
9	26.205054560869545	15.91838970416625	18.9006615892431	38.9758941457211
10	15.606647328636955	27.0333509566609	32.73271127750073	24.627290437201417
11	35.93217876722442	21.6410890263826	23.418901733278084	19.0078304731149
12	24.394733959199616	22.796369594520574	29.605999552272216	23.202896894007594
13	28.882371433062314	20.299572753382964	25.512981724133006	25.305074089421716
14	23.04512048164078	19.58594706333443	25.634677945596312	31.734254509428478
15	24.650748515115577	27.365217267050568	22.346260282259024	25.63777393557483
16	25.100619674301853	26.38474105616126	23.978204230551228	24.53643503898566
17	24.209450866639042	26.23732430256872	25.582046115961493	23.971178714830746
18	24.351151946425084	26.203030259729744	25.767567361597337	23.678250432247832
19	25.057156738064958	25.72219920075828	25.51286264759537	23.707781413581394
20	24.880447156214128	25.614673087273577	25.41533896327203	24.089540793240264
21	24.541674406641615	25.388546742304086	25.530485975165394	24.539292875888908
22	24.688495777545977	25.057156738064958	25.353300087164026	24.90104739722504
23	24.480349989759418	25.257086244754678	25.449871159186277	24.812692606299624
24	24.354962395629414	25.65134866086526	25.33329522884129	24.660393714664036
25	24.81507413705233	25.1948092155714	25.292690129507644	24.697426517868625
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	4.0
1	4.0
2	11.5
3	19.0
4	19.0
5	19.0
6	93.0
7	167.0
8	167.0
9	167.0
10	510.5
11	854.0
12	854.0
13	854.0
14	1359.5
15	1865.0
16	1865.0
17	1865.0
18	3452.0
19	5039.0
20	5039.0
21	5039.0
22	8085.5
23	11132.0
24	11132.0
25	11132.0
26	15907.0
27	20682.0
28	20682.0
29	20682.0
30	27658.5
31	34635.0
32	34635.0
33	34635.0
34	43457.0
35	52279.0
36	52279.0
37	52279.0
38	64525.0
39	76771.0
40	76771.0
41	76771.0
42	86986.5
43	97202.0
44	97202.0
45	97202.0
46	105216.5
47	113231.0
48	113231.0
49	113231.0
50	115077.5
51	116924.0
52	116924.0
53	116924.0
54	112552.5
55	108181.0
56	108181.0
57	108181.0
58	96815.0
59	85449.0
60	85449.0
61	85449.0
62	71915.0
63	58381.0
64	58381.0
65	58381.0
66	45586.5
67	32792.0
68	32792.0
69	32792.0
70	24100.0
71	15408.0
72	15408.0
73	15408.0
74	10729.0
75	6050.0
76	6050.0
77	6050.0
78	4036.0
79	2022.0
80	2022.0
81	2022.0
82	1294.0
83	566.0
84	566.0
85	566.0
86	343.0
87	120.0
88	120.0
89	120.0
90	68.5
91	17.0
92	17.0
93	17.0
94	11.5
95	6.0
96	6.0
97	6.0
98	3.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	839796.0
>>END_MODULE
>>Sequence Duplication Levels	pass
#Total Deduplicated Percentage	80.5329822029713
#Duplication Level	Percentage of deduplicated	Percentage of total
1	90.62431485217623	72.98246335146776
2	5.745795922839463	9.254521615918712
3	1.556800704018409	3.761214101708632
4	0.6936481060613181	2.2344620232224353
5	0.3859569494048523	1.5541132068767034
6	0.22847304805922297	1.1039769547927207
7	0.15773918131429787	0.8892244677046931
8	0.10602889588989763	0.6831058548561458
9	0.075293737311041	0.5457266286178736
>10	0.4054181344387486	5.624888153170412
>50	0.01650492192359611	0.8884486581923251
>100	0.00402554656290348	0.4778549834715444
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	1.1907653763533048E-4
10	0.0	0.0	0.0	0.0	1.1907653763533048E-4
11	0.0	0.0	0.0	0.0	1.1907653763533048E-4
12	0.0	0.0	0.0	0.0	2.3815307527066097E-4
13	0.0	0.0	0.0	0.0	2.3815307527066097E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AAGTGCG	35	0.002169083	16.285715	7
AGAACCG	55	1.1270771E-5	15.545455	5
GTATAAA	60	2.5654876E-5	14.250001	1
GGGTAAC	55	1.9584646E-4	13.818182	1
TACTTAT	60	4.0885454E-4	12.666667	7
GCCTTAG	100	1.4393481E-7	12.349999	1
AGCCGTG	55	0.0030664278	12.090909	7
TAACACA	110	3.8040525E-8	12.090909	4
TAGGGTG	80	2.8626859E-5	11.875	5
CTTAGAC	65	8.016685E-4	11.692307	3
GACTGTC	155	2.5465852E-11	11.645162	7
GGAGCGG	75	2.0716511E-4	11.400001	6
ATAACAC	85	5.3158758E-5	11.176471	3
CAATACA	85	5.3158758E-5	11.176471	4
TCAGGAC	120	1.2719102E-7	11.083334	3
ATCTTAC	60	0.005871847	11.083334	1
ATAAGGC	60	0.005871847	11.083334	3
ACGCAGA	105	3.4594195E-6	10.857143	1
ATGGAAC	80	3.7667857E-4	10.6875	3
ACAGATC	100	2.394772E-5	10.45	8
>>END_MODULE