FastQCFastQC Report
Mon 15 Aug 2016
SRR2920800_2.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920800_2.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584668
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
GTTCATA456.753406E-414.7777791
ATTCACG400.005273891614.2500019
CGTCAGG400.005273891614.25000114
TCTACAC801.993325E-613.0625013
CCTTACT802.8592007E-511.8750011
CCGCTCC658.010296E-411.6923079
GTATTCC752.0695991E-411.4000011
TCTTAGG752.0695991E-411.4000012
CTTGAGC752.0695991E-411.4000013
AGGAGTG600.00586823111.0833345
GCTTATT600.00586823111.0833341
TTCATAT600.00586823111.0833342
TTACTGA700.001488992710.8571433
GTACATG1752.0008883E-1110.8571421
AGGACTG1252.228935E-710.645
GTTACAC750.002643348610.1333343
TGCTAGG856.5798464E-410.0588242
CTATGCT951.6398434E-410.0000014
ATAGCAC1054.0923172E-59.9523813
CCCTTGG1356.4103006E-79.8518511
Produced by FastQC (version 0.11.5)