##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920800_2.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	584668
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.32810244446421	33.0	32.0	34.0	32.0	34.0
2	32.46391456347876	33.0	32.0	34.0	32.0	34.0
3	32.69347219276581	33.0	32.0	34.0	32.0	34.0
4	32.80756258252547	33.0	33.0	34.0	32.0	34.0
5	32.76841900018472	33.0	33.0	34.0	32.0	34.0
6	35.38282409846272	37.0	34.0	37.0	33.0	37.0
7	35.36913427791499	37.0	34.0	37.0	33.0	37.0
8	35.404186649517335	37.0	34.0	37.0	33.0	37.0
9	35.41849220412268	37.0	34.0	37.0	33.0	37.0
10	35.43539239363194	37.0	34.0	37.0	33.0	37.0
11	35.380241436165484	37.0	34.0	37.0	33.0	37.0
12	35.320941115299625	37.0	34.0	37.0	33.0	37.0
13	37.131618285933214	38.0	38.0	38.0	37.0	38.0
14	37.17565524365965	38.0	38.0	38.0	37.0	38.0
15	37.13048772978853	38.0	38.0	38.0	37.0	38.0
16	37.117035651001935	38.0	38.0	38.0	37.0	38.0
17	37.10588744381427	38.0	38.0	38.0	37.0	38.0
18	37.088330471310215	38.0	38.0	38.0	37.0	38.0
19	37.11707841031149	38.0	38.0	38.0	37.0	38.0
20	37.135759097470704	38.0	38.0	38.0	37.0	38.0
21	37.13967927097087	38.0	38.0	38.0	37.0	38.0
22	37.02930381002552	38.0	38.0	38.0	37.0	38.0
23	37.66240840955893	39.0	38.0	39.0	37.0	39.0
24	37.70589462737827	39.0	38.0	39.0	37.0	39.0
25	37.518302694862726	39.0	38.0	39.0	36.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
17	2.0
18	32.0
19	112.0
20	270.0
21	584.0
22	1021.0
23	1439.0
24	1972.0
25	2499.0
26	3124.0
27	3598.0
28	4239.0
29	5095.0
30	5930.0
31	7300.0
32	9609.0
33	13450.0
34	23092.0
35	68647.0
36	355915.0
37	76738.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.16654237960689	15.921343394883932	11.271353999192705	26.640760226316473
2	18.524701197944818	19.17276129358884	35.35237091819631	26.950166590270037
3	20.71141228868349	21.763633378259115	27.618751154501357	29.906203178556034
4	14.684231050784376	15.09831220453317	33.76018526753645	36.457271477146
5	16.132403346856677	34.116797909240795	32.97991338674256	16.770885357159962
6	35.16405891890783	32.570450238425906	17.01324512372834	15.252245718937926
7	30.447022925831412	28.483344393741405	20.93991803895544	20.12971464147174
8	27.367668488783377	32.27062195981309	19.980399132499127	20.381310418904402
9	26.302277531864238	15.770488550767272	18.322535182359903	39.60469873500859
10	16.41700931126725	25.571264375679874	31.74519556397819	26.26653074907469
11	36.34866283087154	21.098640596030567	23.009468621508276	19.54322795158962
12	25.428961393474587	21.96152346288834	28.242010850602394	24.36750429303468
13	29.357173643845734	19.750696121559585	24.913797231933337	25.97833300266134
14	24.17200872974064	18.86882812125856	24.467903151874225	32.491259997126576
15	25.492929320571672	26.070693111304195	21.700178562876708	26.736199005247425
16	25.998857471248638	25.03540470831309	23.202911737943584	25.762826082494684
17	25.193272079197083	24.99042191465926	24.54880376555584	25.26750224058782
18	25.26750224058782	24.8327255810135	24.97673893560106	24.923033242797622
19	25.92052241614044	24.41607886869129	24.911060636121697	24.75233807904657
20	25.849712999514253	24.484493763982293	24.335520329486133	25.330272907017314
21	25.401082323643504	24.32713950481299	24.54863272831761	25.7231454432259
22	25.485403682089665	24.025429816579667	24.207755512530188	26.28141098880048
23	25.262200086202768	24.145668995053605	24.650399885062978	25.941731033680654
24	25.281014182407795	24.761232015434402	24.345953601018014	25.61180020113979
25	25.77685113602934	24.11026428674051	24.469955598732955	25.6429289784972
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	6.5
3	11.0
4	11.0
5	11.0
6	29.0
7	47.0
8	47.0
9	47.0
10	137.0
11	227.0
12	227.0
13	227.0
14	465.5
15	704.0
16	704.0
17	704.0
18	1234.5
19	1765.0
20	1765.0
21	1765.0
22	2926.5
23	4088.0
24	4088.0
25	4088.0
26	6404.5
27	8721.0
28	8721.0
29	8721.0
30	12313.0
31	15905.0
32	15905.0
33	15905.0
34	21937.0
35	27969.0
36	27969.0
37	27969.0
38	37182.0
39	46395.0
40	46395.0
41	46395.0
42	54970.0
43	63545.0
44	63545.0
45	63545.0
46	71681.5
47	79818.0
48	79818.0
49	79818.0
50	83008.0
51	86198.0
52	86198.0
53	86198.0
54	85532.5
55	84867.0
56	84867.0
57	84867.0
58	76321.0
59	67775.0
60	67775.0
61	67775.0
62	58016.0
63	48257.0
64	48257.0
65	48257.0
66	38152.5
67	28048.0
68	28048.0
69	28048.0
70	20691.5
71	13335.0
72	13335.0
73	13335.0
74	9220.5
75	5106.0
76	5106.0
77	5106.0
78	3279.5
79	1453.0
80	1453.0
81	1453.0
82	893.0
83	333.0
84	333.0
85	333.0
86	207.0
87	81.0
88	81.0
89	81.0
90	48.5
91	16.0
92	16.0
93	16.0
94	8.5
95	1.0
96	1.0
97	1.0
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	584668.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	65.84074447447854
#Duplication Level	Percentage of deduplicated	Percentage of total
1	86.1444453522413	56.718144143326
2	7.040938324258013	9.271612421360699
3	2.37517617860805	4.69150103572803
4	1.185986156989936	3.1234484605057267
5	0.7316200957232786	2.4085205887454957
6	0.47701747970778563	1.884431159478004
7	0.359947296370788	1.6589438575239912
8	0.27128677751750635	1.4289378718267873
9	0.19020787019208588	1.1271085000516718
>10	1.1490531566347224	13.609933436808669
>50	0.06116395035754638	2.7601887453737217
>100	0.013157361399108135	1.317229779271141
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	0.0
2	0.0	0.0	0.0	0.0	0.0
3	0.0	0.0	0.0	0.0	0.0
4	0.0	0.0	0.0	0.0	0.0
5	0.0	0.0	0.0	0.0	0.0
6	0.0	0.0	0.0	0.0	0.0
7	0.0	0.0	0.0	0.0	0.0
8	0.0	0.0	0.0	0.0	0.0
9	0.0	0.0	0.0	0.0	0.0
10	0.0	0.0	0.0	0.0	0.0
11	0.0	0.0	0.0	0.0	0.0
12	0.0	0.0	0.0	1.710372382275069E-4	0.0
13	0.0	0.0	0.0	1.710372382275069E-4	1.710372382275069E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GTTCATA	45	6.753406E-4	14.777779	1
ATTCACG	40	0.0052738916	14.250001	9
CGTCAGG	40	0.0052738916	14.250001	14
TCTACAC	80	1.993325E-6	13.062501	3
CCTTACT	80	2.8592007E-5	11.875001	1
CCGCTCC	65	8.010296E-4	11.692307	9
GTATTCC	75	2.0695991E-4	11.400001	1
TCTTAGG	75	2.0695991E-4	11.400001	2
CTTGAGC	75	2.0695991E-4	11.400001	3
AGGAGTG	60	0.005868231	11.083334	5
GCTTATT	60	0.005868231	11.083334	1
TTCATAT	60	0.005868231	11.083334	2
TTACTGA	70	0.0014889927	10.857143	3
GTACATG	175	2.0008883E-11	10.857142	1
AGGACTG	125	2.228935E-7	10.64	5
GTTACAC	75	0.0026433486	10.133334	3
TGCTAGG	85	6.5798464E-4	10.058824	2
CTATGCT	95	1.6398434E-4	10.000001	4
ATAGCAC	105	4.0923172E-5	9.952381	3
CCCTTGG	135	6.4103006E-7	9.851851	1
>>END_MODULE