FastQCFastQC Report
Mon 15 Aug 2016
SRR2920800_1.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2920800_1.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences584668
Sequences flagged as poor quality0
Sequence length25
%GC51

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[OK]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[WARN]Sequence Duplication Levels

Duplication level graph

[OK]Overrepresented sequences

No overrepresented sequences

[OK]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TTAAGTA402.7574218E-416.6250024
GGGATAC350.002168022316.2857151
GCAGTCG400.005273891614.25000119
TATAAGA400.005273891614.2500012
GTGCTAT551.9568621E-413.8181821
GCCTTAG604.085244E-412.6666671
GTGTGTT853.931442E-612.2941187
GACCGTG701.0886572E-412.2142867
CTTAATC550.003064510612.0909093
GATACCC550.003064510612.0909093
TCTGAAC550.003064510612.0909093
ATATCCT550.003064510612.0909094
AAATTTT550.003064510612.09090915
TATCCTC951.0360818E-612.0000015
AGACCGT658.010296E-411.6923076
GTATAAG658.010296E-411.6923071
TTTACGG752.0695991E-411.40000118
CTTTACG752.0695991E-411.40000117
GTTATCC752.0695991E-411.4000013
TAGCTCT600.00586823111.0833346
Produced by FastQC (version 0.11.5)