##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2920800_1.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	584668
Sequences flagged as poor quality	0
Sequence length	25
%GC	51
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	33.183150095438776	33.0	33.0	34.0	32.0	34.0
2	33.34506078663446	34.0	33.0	34.0	32.0	35.0
3	33.512851738080414	34.0	33.0	34.0	32.0	35.0
4	33.57776721147728	34.0	33.0	34.0	32.0	35.0
5	33.496524523319216	34.0	33.0	34.0	32.0	35.0
6	35.98632557280371	37.0	36.0	37.0	33.0	37.0
7	36.047019847161124	37.0	37.0	37.0	34.0	37.0
8	36.007838636627966	37.0	36.0	37.0	34.0	37.0
9	35.976937338797406	37.0	35.0	37.0	33.0	37.0
10	35.93469285132759	37.0	35.0	37.0	33.0	37.0
11	35.85086921124467	37.0	35.0	37.0	33.0	37.0
12	35.85471583873241	37.0	35.0	37.0	33.0	37.0
13	37.611718103265446	38.0	38.0	38.0	38.0	38.0
14	37.605388015078645	38.0	38.0	38.0	38.0	38.0
15	37.596918251041615	38.0	38.0	38.0	38.0	38.0
16	37.58651576621262	38.0	38.0	38.0	38.0	38.0
17	37.60617649674687	38.0	38.0	38.0	38.0	38.0
18	37.631611444443685	38.0	38.0	38.0	38.0	38.0
19	37.63415818892089	38.0	38.0	38.0	38.0	38.0
20	37.59151518468601	38.0	38.0	38.0	38.0	38.0
21	37.55940636395356	38.0	38.0	38.0	38.0	38.0
22	37.607568739866046	38.0	38.0	38.0	38.0	38.0
23	38.34121415914673	39.0	38.0	39.0	38.0	39.0
24	38.289793523846015	39.0	38.0	39.0	38.0	39.0
25	38.25056271251377	39.0	38.0	39.0	38.0	39.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
21	1.0
22	3.0
23	22.0
24	48.0
25	156.0
26	285.0
27	616.0
28	1027.0
29	1689.0
30	2651.0
31	3808.0
32	5667.0
33	8733.0
34	15758.0
35	49975.0
36	365880.0
37	128349.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.91648593731827	16.06005459508644	11.273748520527889	26.749710947067395
2	18.531713724712144	19.22834839601278	35.27814075680557	26.961797122469505
3	20.85833327632092	21.744306170339406	27.4903021885925	29.907058364747176
4	14.921117625729474	15.264560400090307	33.63395978572455	36.180362188455675
5	16.089301962823345	34.23566878980891	32.968453891781316	16.706575355586416
6	35.36468559934869	32.55488584974721	16.84768107712411	15.232747473779993
7	30.665608516286163	28.626160487661373	20.811811147523038	19.896419848529423
8	27.38973229251473	32.53607175354218	19.782338010631676	20.291857943311417
9	26.360943304576274	15.760739428188305	18.30833909158702	39.5699781756484
10	16.332174841106408	25.796007306710816	31.711330190809147	26.160487661373633
11	36.52910711720156	20.990545061470783	22.95353944460788	19.526808376719778
12	25.46590543693173	21.972640883373128	28.306149814937708	24.255303864757437
13	29.40711651740817	19.670308619592657	24.9266250248004	25.995949838198772
14	24.090423967106116	18.728577585912003	24.4832965033147	32.69770194366718
15	25.56579118405659	26.032893881655916	21.722584441084514	26.67873049320298
16	26.07103518578065	25.001197260667592	23.331189666614215	25.596577886937546
17	25.359007163039536	24.94578119548188	24.52109573296298	25.174115908515603
18	25.194982451579357	24.88335260352884	24.940821115573282	24.98084382931852
19	25.954729863785943	24.42599902850849	24.925598801371034	24.693672306334534
20	25.802848796239918	24.539225680215097	24.420012725170523	25.237912798374463
21	25.456669426067442	24.287800940020663	24.679476215561653	25.57605341835024
22	25.393385647923267	24.107185616452416	24.333125808150953	26.166302927473367
23	25.208323356161106	24.030389896488263	24.746009701232154	26.015277046118477
24	25.28375077821943	24.781927521259927	24.42240724650571	25.511914454014928
25	25.824057413780128	24.15969404858826	24.50826794009592	25.507980597535695
>>END_MODULE
>>Per sequence GC content	pass
#GC Content	Count
0	2.0
1	2.0
2	4.0
3	6.0
4	6.0
5	6.0
6	33.5
7	61.0
8	61.0
9	61.0
10	234.0
11	407.0
12	407.0
13	407.0
14	557.0
15	707.0
16	707.0
17	707.0
18	1222.0
19	1737.0
20	1737.0
21	1737.0
22	3005.5
23	4274.0
24	4274.0
25	4274.0
26	6446.5
27	8619.0
28	8619.0
29	8619.0
30	12297.5
31	15976.0
32	15976.0
33	15976.0
34	21855.5
35	27735.0
36	27735.0
37	27735.0
38	36919.5
39	46104.0
40	46104.0
41	46104.0
42	54802.5
43	63501.0
44	63501.0
45	63501.0
46	71702.0
47	79903.0
48	79903.0
49	79903.0
50	83119.5
51	86336.0
52	86336.0
53	86336.0
54	85851.0
55	85366.0
56	85366.0
57	85366.0
58	76684.0
59	68002.0
60	68002.0
61	68002.0
62	58239.5
63	48477.0
64	48477.0
65	48477.0
66	37894.0
67	27311.0
68	27311.0
69	27311.0
70	20248.0
71	13185.0
72	13185.0
73	13185.0
74	9146.0
75	5107.0
76	5107.0
77	5107.0
78	3267.0
79	1427.0
80	1427.0
81	1427.0
82	880.5
83	334.0
84	334.0
85	334.0
86	204.5
87	75.0
88	75.0
89	75.0
90	45.0
91	15.0
92	15.0
93	15.0
94	8.0
95	1.0
96	1.0
97	1.0
98	0.5
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
25	584668.0
>>END_MODULE
>>Sequence Duplication Levels	warn
#Total Deduplicated Percentage	64.5979780562024
#Duplication Level	Percentage of deduplicated	Percentage of total
1	85.4250013544122	55.182823629433784
2	7.372009875809046	9.524338643752401
3	2.5147463414604996	4.873425869477418
4	1.2521542884522978	3.2354654099368494
5	0.764643560308105	2.469721396479972
6	0.5356868360903058	2.0762571889654837
7	0.3648689643587335	1.6498858147124331
8	0.27671610595411	1.4300240752177098
9	0.21107862583173714	1.2271727195650717
>10	1.202869543485452	13.932020210084497
>50	0.06417960307790259	2.8368604323810076
>100	0.01604490075960743	1.5620046099933886
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	pass
>>END_MODULE
>>Adapter Content	pass
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.0	1.710372382275069E-4
2	0.0	0.0	0.0	0.0	1.710372382275069E-4
3	0.0	0.0	0.0	0.0	1.710372382275069E-4
4	0.0	0.0	0.0	0.0	1.710372382275069E-4
5	0.0	0.0	0.0	0.0	1.710372382275069E-4
6	0.0	0.0	0.0	0.0	1.710372382275069E-4
7	0.0	0.0	0.0	0.0	1.710372382275069E-4
8	0.0	0.0	0.0	0.0	1.710372382275069E-4
9	0.0	0.0	0.0	0.0	1.710372382275069E-4
10	0.0	0.0	0.0	0.0	1.710372382275069E-4
11	0.0	0.0	0.0	0.0	1.710372382275069E-4
12	0.0	0.0	0.0	0.0	1.710372382275069E-4
13	0.0	0.0	0.0	0.0	1.710372382275069E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTAAGTA	40	2.7574218E-4	16.625002	4
GGGATAC	35	0.0021680223	16.285715	1
GCAGTCG	40	0.0052738916	14.250001	19
TATAAGA	40	0.0052738916	14.250001	2
GTGCTAT	55	1.9568621E-4	13.818182	1
GCCTTAG	60	4.085244E-4	12.666667	1
GTGTGTT	85	3.931442E-6	12.294118	7
GACCGTG	70	1.0886572E-4	12.214286	7
CTTAATC	55	0.0030645106	12.090909	3
GATACCC	55	0.0030645106	12.090909	3
TCTGAAC	55	0.0030645106	12.090909	3
ATATCCT	55	0.0030645106	12.090909	4
AAATTTT	55	0.0030645106	12.090909	15
TATCCTC	95	1.0360818E-6	12.000001	5
AGACCGT	65	8.010296E-4	11.692307	6
GTATAAG	65	8.010296E-4	11.692307	1
TTTACGG	75	2.0695991E-4	11.400001	18
CTTTACG	75	2.0695991E-4	11.400001	17
GTTATCC	75	2.0695991E-4	11.400001	3
TAGCTCT	60	0.005868231	11.083334	6
>>END_MODULE