SRR2936814.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936814.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	80943
Sequences flagged as poor quality	0
Sequence length	51
%GC	41

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1387	1.7135515115575157	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTC	898	1.109422680157642	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCG	825	1.0192357584967198	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	777	0.9599347689114561	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	726	0.8969274674771136	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCG	569	0.7029638140419802	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC	563	0.6955511903438222	No Hit
GAATCTATCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTC	555	0.6856676920796115	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC	394	0.4867622895123729	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	315	0.38916274415329305	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	290	0.35827681207763484	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTT	197	0.24338114475618644	No Hit
GAATATGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTC	195	0.2409102701901338	No Hit
GAATCTATCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTT	193	0.23843939562408117	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCT	193	0.23843939562408117	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCG	190	0.23473308377500215	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCT	188	0.23226220920894952	No Hit
GCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	161	0.19890540256723865	TruSeq Adapter, Index 16 (95% over 21bp)
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	141	0.17419665690671213	No Hit
GAATGAATGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCT	129	0.1593714095103962	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTC	129	0.1593714095103962	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTT	126	0.15566509766131723	No Hit
GAACTGACTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCT	119	0.14701703668013294	No Hit
GAATGCAGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTT	110	0.135898101132896	No Hit
GCTGTCACTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTCTTCTGC	102	0.12601460286868538	TruSeq Adapter, Index 16 (95% over 21bp)
GAATGAACTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGCCGTC	98	0.12107285373658006	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGCTGTCTTTCGTATGC	82	0.10130585720815884	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGGTAT	20	6.9944264E-4	45.000004	6
CATGGGA	20	6.9944264E-4	45.000004	4
GAATGAC	20	6.9944264E-4	45.000004	1
TCATTAA	20	6.9944264E-4	45.000004	17
CGGTTTA	20	6.9944264E-4	45.000004	1
GTGGGAC	20	6.9944264E-4	45.000004	5
TTGTCCC	20	6.9944264E-4	45.000004	21
AACGGCG	20	6.9944264E-4	45.000004	7
CAAGGAT	20	6.9944264E-4	45.000004	5
GGAGTTC	20	6.9944264E-4	45.000004	8
TCGAGGG	20	6.9944264E-4	45.000004	3
AGGATGC	20	6.9944264E-4	45.000004	7
CGTTTTA	220	0.0	45.000004	1
AAGGATT	20	6.9944264E-4	45.000004	6
CTCTACT	20	6.9944264E-4	45.000004	10
CAGCGGA	25	3.8586317E-5	45.0	12
GCAAGAC	25	3.8586317E-5	45.0	45
CTATCTC	100	0.0	45.0	5
CGTGACT	25	3.8586317E-5	45.0	22
GGCCGGC	25	3.8586317E-5	45.0	45

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content