SRR2936807.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936807.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	156131
Sequences flagged as poor quality	0
Sequence length	51
%GC	39

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3551	2.274372161838456	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2040	1.3065951028303155	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	792	0.5072663340400049	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	688	0.440655603307479	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC	638	0.4086312135322261	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG	573	0.36699950682439747	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC	511	0.32728926350308396	No Hit
TCATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	485	0.3106365808199525	No Hit
TATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	471	0.3016697516828817	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	426	0.27284780088515415	No Hit
GAATCTATCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC	418	0.26772389852111367	RNA PCR Primer, Index 40 (95% over 24bp)
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC	348	0.22288975283575974	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG	322	0.20623707015262824	No Hit
GAACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCT	292	0.18702243628747653	RNA PCR Primer, Index 40 (96% over 25bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	255	0.16332438785378944	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTT	222	0.14218829060212257	No Hit
GAATATGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC	216	0.13834536382909224	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	199	0.12745707130550626	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG	175	0.11208536421338491	No Hit
CATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	173	0.1108043886223748	No Hit
GAATCTATCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTT	170	0.10888292523585964	RNA PCR Primer, Index 40 (95% over 23bp)

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	35	1.2029341E-7	45.000004	23
TACCCGC	35	1.2029341E-7	45.000004	28
TGAAACG	75	0.0	45.000004	4
AACGGGT	35	1.2029341E-7	45.000004	21
AAACGGC	75	0.0	45.000004	6
GTTACCC	35	1.2029341E-7	45.000004	26
TTACCCG	35	1.2029341E-7	45.000004	27
GGTTCAA	35	1.2029341E-7	45.000004	16
GGTTACC	35	1.2029341E-7	45.000004	25
TCAACGG	35	1.2029341E-7	45.000004	19
CCGGCGT	25	3.8752798E-5	45.0	40
GCCGGCC	20	7.0145464E-4	45.0	43
TTCAACG	40	6.7502697E-9	45.0	18
CACGAAT	25	3.8752798E-5	45.0	7
CGCGAAG	25	3.8752798E-5	45.0	2
GGCCCGA	25	3.8752798E-5	45.0	24
CGTTATT	245	0.0	45.0	1
GGGACGT	20	7.0145464E-4	45.0	8
CGGATAG	20	7.0145464E-4	45.0	2
ACGTCCC	20	7.0145464E-4	45.0	7

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content