##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936807.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	156131
Sequences flagged as poor quality	0
Sequence length	51
%GC	39
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.680050726633404	31.0	31.0	34.0	30.0	34.0
2	31.829124261037204	31.0	31.0	34.0	30.0	34.0
3	31.223991391844027	31.0	31.0	34.0	28.0	34.0
4	35.30282903459275	37.0	35.0	37.0	33.0	37.0
5	31.72002997482883	37.0	35.0	37.0	0.0	37.0
6	33.67575945840352	37.0	35.0	37.0	17.0	37.0
7	20.947480000768586	32.0	0.0	37.0	0.0	37.0
8	28.02243628747654	35.0	17.0	37.0	17.0	37.0
9	34.51064170472232	35.0	32.0	39.0	28.0	39.0
10	36.17395008038122	37.0	35.0	39.0	32.0	39.0
11	36.66136129276057	38.0	35.0	39.0	33.0	39.0
12	36.989489595275764	39.0	37.0	39.0	33.0	39.0
13	36.79588294445049	39.0	37.0	39.0	33.0	39.0
14	38.27557627889401	40.0	38.0	41.0	34.0	41.0
15	38.36911952142752	40.0	38.0	41.0	34.0	41.0
16	38.419621984103095	40.0	38.0	41.0	34.0	41.0
17	38.32265213186363	40.0	37.0	41.0	34.0	41.0
18	38.109267217913164	40.0	37.0	41.0	33.0	41.0
19	37.98651773190462	40.0	37.0	41.0	34.0	41.0
20	37.86741902633045	40.0	36.0	41.0	33.0	41.0
21	37.82110535383748	39.0	36.0	41.0	33.0	41.0
22	37.68780062895902	39.0	35.0	41.0	33.0	41.0
23	37.59284831327539	39.0	35.0	41.0	33.0	41.0
24	37.326642370829624	39.0	35.0	41.0	33.0	41.0
25	37.178753738847504	39.0	35.0	41.0	33.0	41.0
26	37.053435896778986	39.0	35.0	40.0	33.0	41.0
27	37.0266891264387	39.0	35.0	40.0	33.0	41.0
28	36.91990700117209	39.0	35.0	40.0	33.0	41.0
29	36.82657511961109	39.0	35.0	40.0	32.0	41.0
30	36.585027957292276	38.0	35.0	40.0	31.0	41.0
31	36.39846667221756	38.0	35.0	40.0	31.0	41.0
32	36.23937590869206	38.0	35.0	40.0	30.0	41.0
33	35.953820829944085	38.0	35.0	40.0	30.0	41.0
34	35.662283595186096	38.0	35.0	40.0	27.0	41.0
35	35.348470194900436	38.0	35.0	40.0	25.0	41.0
36	35.05676002843766	38.0	34.0	40.0	24.0	41.0
37	34.886242962640345	38.0	34.0	40.0	23.0	41.0
38	34.6025645131332	38.0	34.0	40.0	21.0	41.0
39	34.259391152301596	38.0	33.0	40.0	18.0	41.0
40	33.93173681075507	38.0	33.0	40.0	15.0	41.0
41	33.82311008063741	38.0	33.0	40.0	15.0	41.0
42	33.66077844886666	38.0	33.0	40.0	15.0	41.0
43	33.46676829073022	38.0	33.0	40.0	15.0	41.0
44	33.25875066450609	37.0	33.0	40.0	15.0	41.0
45	33.238946781869075	37.0	33.0	40.0	15.0	41.0
46	32.86935329947288	37.0	31.0	40.0	12.0	41.0
47	32.70975014571097	37.0	31.0	40.0	12.0	41.0
48	32.59539104982355	37.0	31.0	40.0	11.0	41.0
49	32.484727568516185	36.0	31.0	40.0	10.0	41.0
50	32.33613439995901	36.0	31.0	40.0	10.0	41.0
51	30.563405089316024	35.0	26.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	1.0
9	19.0
10	6.0
11	11.0
12	8.0
13	1.0
14	7.0
15	9.0
16	12.0
17	34.0
18	80.0
19	135.0
20	226.0
21	434.0
22	708.0
23	1223.0
24	1925.0
25	2861.0
26	3699.0
27	4131.0
28	4259.0
29	4270.0
30	4583.0
31	5181.0
32	6341.0
33	8802.0
34	11540.0
35	14535.0
36	14993.0
37	23715.0
38	25492.0
39	16886.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.8485886851426	17.149701212443397	20.580794332963986	22.420915769450016
2	37.28151360075834	27.753617154825115	19.36002459473135	15.604844649685202
3	26.817864485592224	26.95620984942132	32.54318488961193	13.682740775374524
4	24.14446842715412	20.545567504211206	39.49119649525078	15.818767573383887
5	20.962525059085	31.933440508291117	29.010254209606035	18.09378022301785
6	21.782989925126977	33.19968488000461	32.75838878890163	12.258936405966784
7	37.25205116216511	43.717775457788655	15.20069685072151	3.829476529324734
8	62.49623713420141	11.229032030794652	21.082296276844446	5.192434558159494
9	58.017946468030054	10.66348130736369	20.95740115672096	10.361171067885302
10	31.08095125247388	27.45450935432425	28.398588364898707	13.065951028303155
11	24.498658178068418	25.163484509802664	32.75646732551511	17.581389986613804
12	23.316317707566082	23.589805996246742	37.528741889823294	15.565134406363887
13	18.843150943758765	25.814860597831306	39.15814284158816	16.18384561682177
14	14.740186125753374	31.88476343583273	35.37478143353978	18.000269004874113
15	15.315344166116915	28.912900064689268	41.06551549660221	14.706240272591605
16	17.087573896279405	27.46860008582536	37.65940140010632	17.784424617788908
17	16.737227072138143	28.230140074680875	33.888209260172545	21.144423593008437
18	16.71737195047748	30.84973515829656	34.63886095650447	17.79403193472148
19	18.27119534237275	30.289308337229638	32.584816596319754	18.854679724077858
20	19.402937277030187	28.399228852694215	35.55155606509918	16.646277805176425
21	20.28873189821368	28.616354215370425	34.388430228462	16.706483657953896
22	18.02076461433027	25.346023531521606	34.65871607816513	21.974495775982987
23	18.386483145563663	29.36252249713382	35.61880728362721	16.63218707367531
24	20.341892385240598	27.106083993569502	34.533820958041645	18.01820266314825
25	19.16659728048882	30.767752720471915	33.022269760649706	17.043380238389556
26	17.20734511403885	27.503826914578145	33.91510974758376	21.373718223799244
27	18.64203777597018	28.588813240163706	33.81327218809845	18.955876795767658
28	15.976327571078134	27.633205449270164	37.655558473333286	18.734908506318412
29	18.494725583004016	27.89836739660926	35.57653508912388	18.030371931262852
30	16.727619755205563	29.0813483549071	35.694384843496806	18.496647046390528
31	20.736432867271716	30.73060442833262	31.224420518667017	17.30854218572865
32	21.910446996432484	30.562156138114787	30.688332233829286	16.839064631623444
33	17.817089495359667	30.501309797541808	30.678084429101204	21.00351627799732
34	17.908679250116887	29.93383761072433	32.7045878140792	19.45289532507958
35	18.29169095182891	27.537132279944405	31.023947838673934	23.147228929552746
36	18.77269728625321	29.78588492996266	31.52737124594091	19.914046537843223
37	18.72786314056786	28.30699861014148	32.96590683464527	19.999231414645394
38	17.16955633410405	32.22934586981445	30.707546867694436	19.89355092838706
39	20.34317336083161	29.18510737777892	30.71843516021802	19.753284101171452
40	18.63819484919715	28.26985031800219	34.685616565576346	18.406338267224317
41	16.979971946634556	29.881317611492914	32.94797317637113	20.190737265501408
42	17.249617308542188	29.137711280911542	33.23939512332593	20.373276287220346
43	20.066482633173425	27.1182532616841	30.589056625526002	22.226207479616473
44	19.10831289109786	27.726076179618396	32.08907904259885	21.076531886684897
45	19.38564410655155	28.06041080887204	30.13431029071741	22.419634793859004
46	21.26419481076788	29.79933517366827	29.735926881913265	19.200543133650587
47	19.452254837284073	27.637048376043193	34.363451204437304	18.54724558223543
48	19.10703191550685	28.98847762455886	31.347394175403988	20.5570962845303
49	17.236807552632087	28.392183486943658	34.63629900532245	19.734709955101806
50	16.99150072695365	26.60650351307556	35.77956972029898	20.622426039671815
51	17.116395847077133	26.536049855570003	33.58589902069416	22.761655276658704
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	1019.0
1	1509.5
2	2000.0
3	6012.0
4	10024.0
5	6595.5
6	3167.0
7	2490.0
8	1813.0
9	1663.5
10	1514.0
11	1401.0
12	1288.0
13	1253.0
14	1218.0
15	1200.5
16	1183.0
17	1110.0
18	1037.0
19	1005.0
20	973.0
21	1021.0
22	1069.0
23	1106.0
24	1143.0
25	1330.5
26	1721.0
27	1924.0
28	2296.0
29	2668.0
30	3113.0
31	3558.0
32	3892.5
33	4227.0
34	4426.0
35	4625.0
36	5016.5
37	5408.0
38	5738.5
39	6069.0
40	6575.5
41	7082.0
42	7962.5
43	8843.0
44	10465.0
45	12087.0
46	13901.0
47	15715.0
48	13702.0
49	11689.0
50	10665.0
51	9641.0
52	8447.0
53	7253.0
54	6586.0
55	5919.0
56	5517.5
57	5116.0
58	4754.5
59	4393.0
60	4159.0
61	3925.0
62	3362.0
63	2799.0
64	2242.5
65	1686.0
66	1372.5
67	1059.0
68	846.5
69	634.0
70	514.0
71	394.0
72	299.5
73	205.0
74	158.5
75	86.5
76	61.0
77	46.0
78	31.0
79	24.5
80	18.0
81	13.5
82	9.0
83	8.0
84	7.0
85	4.0
86	1.0
87	3.0
88	5.0
89	3.0
90	1.0
91	1.0
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	156131.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	45.57198762577579
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.97756914774006	33.257328781600066
2	9.807173375309198	8.93864767406857
3	4.993534967393749	6.8269594122883985
4	3.20159658196537	5.836124792642076
5	2.1320553181920396	4.858099928905855
6	1.6640431751742748	4.550025299267922
7	1.2634922419608725	4.030589697113322
8	0.9838093096469529	3.5867316548283172
9	0.6802338655273218	2.7899648372200265
>10	2.196705644254554	14.270708571648166
>50	0.05200134922419609	1.6473346100390058
>100	0.0379469305149539	3.775675554502309
>500	0.007027209354621093	2.0508419212071916
>1k	0.002810883741848437	3.5809672646687716
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	3551	2.274372161838456	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2040	1.3065951028303155	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	792	0.5072663340400049	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	688	0.440655603307479	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC	638	0.4086312135322261	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG	573	0.36699950682439747	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC	511	0.32728926350308396	No Hit
TCATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	485	0.3106365808199525	No Hit
TATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	471	0.3016697516828817	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	426	0.27284780088515415	No Hit
GAATCTATCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC	418	0.26772389852111367	RNA PCR Primer, Index 40 (95% over 24bp)
GAATGAAACGGCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGC	348	0.22288975283575974	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG	322	0.20623707015262824	No Hit
GAACTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTCT	292	0.18702243628747653	RNA PCR Primer, Index 40 (96% over 25bp)
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	255	0.16332438785378944	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTT	222	0.14218829060212257	No Hit
GAATATGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTC	216	0.13834536382909224	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	199	0.12745707130550626	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCG	175	0.11208536421338491	No Hit
CATTCAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	173	0.1108043886223748	No Hit
GAATCTATCTCTTATACACATCTGACGCTCAGAACCTCGTATGCCGTCTTT	170	0.10888292523585964	RNA PCR Primer, Index 40 (95% over 23bp)
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	6.404877955050566E-4	0.0	0.0	0.07429658427858657	0.0
2	6.404877955050566E-4	0.0	0.0	0.45730828599061046	0.0
3	6.404877955050566E-4	0.0	0.0	0.678276575439855	0.0
4	6.404877955050566E-4	0.0	0.0	1.1810594949113244	0.0
5	6.404877955050566E-4	0.0	0.0	2.619595083615682	0.0
6	6.404877955050566E-4	0.0	0.0	3.2286989771409904	0.0
7	6.404877955050566E-4	0.0	0.0	3.741729701340541	0.0
8	6.404877955050566E-4	0.0	0.0	4.413601398825345	0.0
9	6.404877955050566E-4	0.0	0.0	4.617276517795953	0.0
10	6.404877955050566E-4	0.0	0.0	6.080150642729503	0.0
11	6.404877955050566E-4	0.0	0.0	6.493905758625769	0.0
12	6.404877955050566E-4	0.0	0.0	7.6884154972427	0.0
13	6.404877955050566E-4	0.0	0.0	7.851099397300985	0.0
14	6.404877955050566E-4	0.0	0.0	7.964465737105379	0.0
15	6.404877955050566E-4	0.0	0.0	8.127149637163663	0.0
16	6.404877955050566E-4	0.0	0.0	8.30648621990508	0.0
17	6.404877955050566E-4	0.0	0.0	8.47237255894089	0.0
18	6.404877955050566E-4	0.0	0.0	8.677969141298012	0.0
19	6.404877955050566E-4	0.0	0.0	8.8028642614215	0.0
20	6.404877955050566E-4	0.0	0.0	8.934164259500035	0.0
21	6.404877955050566E-4	0.0	0.0	9.102612549717865	0.0
22	6.404877955050566E-4	0.0	0.0	9.244800840319988	0.0
23	6.404877955050566E-4	0.0	0.0	9.390191569899635	0.0
24	6.404877955050566E-4	0.0	0.0	9.506760348681556	0.0
25	6.404877955050566E-4	0.0	0.0	9.627172054236507	0.0
26	6.404877955050566E-4	0.0	0.0	9.734133516085851	0.0
27	6.404877955050566E-4	0.0	0.0	9.844937904708226	0.0
28	6.404877955050566E-4	0.0	0.0	9.95830424451262	0.0
29	6.404877955050566E-4	0.0	0.0	10.06462521856646	0.0
30	6.404877955050566E-4	0.0	0.0	10.206173021373077	0.0
31	0.0012809755910101132	0.0	0.0	10.3483613119752	0.0
32	0.0012809755910101132	0.0	0.0	10.473896919894191	0.0
33	0.0012809755910101132	0.0	0.0	10.63209740538394	0.0
34	0.0012809755910101132	0.0	0.0	10.767880818031012	0.0
35	0.0012809755910101132	0.0	0.0	10.945936425181419	0.0
36	0.0012809755910101132	0.0	0.0	11.100934471693641	0.0
37	0.0012809755910101132	0.0	0.0	11.231593981976673	0.0
38	0.0012809755910101132	0.0	0.0	11.400682759990008	0.0
39	0.0012809755910101132	0.0	0.0	11.539668611614605	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGTTA	35	1.2029341E-7	45.000004	23
TACCCGC	35	1.2029341E-7	45.000004	28
TGAAACG	75	0.0	45.000004	4
AACGGGT	35	1.2029341E-7	45.000004	21
AAACGGC	75	0.0	45.000004	6
GTTACCC	35	1.2029341E-7	45.000004	26
TTACCCG	35	1.2029341E-7	45.000004	27
GGTTCAA	35	1.2029341E-7	45.000004	16
GGTTACC	35	1.2029341E-7	45.000004	25
TCAACGG	35	1.2029341E-7	45.000004	19
CCGGCGT	25	3.8752798E-5	45.0	40
GCCGGCC	20	7.0145464E-4	45.0	43
TTCAACG	40	6.7502697E-9	45.0	18
CACGAAT	25	3.8752798E-5	45.0	7
CGCGAAG	25	3.8752798E-5	45.0	2
GGCCCGA	25	3.8752798E-5	45.0	24
CGTTATT	245	0.0	45.0	1
GGGACGT	20	7.0145464E-4	45.0	8
CGGATAG	20	7.0145464E-4	45.0	2
ACGTCCC	20	7.0145464E-4	45.0	7
>>END_MODULE