##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936805.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	83528
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.94806531941385	31.0	31.0	34.0	30.0	34.0
2	32.1353677808639	33.0	31.0	34.0	30.0	34.0
3	31.887462886696678	33.0	31.0	34.0	30.0	34.0
4	35.713892347476296	37.0	35.0	37.0	35.0	37.0
5	32.105629250071836	37.0	35.0	37.0	10.0	37.0
6	33.9608753950771	37.0	35.0	37.0	19.0	37.0
7	20.69479695431472	32.0	0.0	37.0	0.0	37.0
8	28.0482592663538	35.0	17.0	37.0	17.0	37.0
9	34.76512067809597	37.0	32.0	39.0	31.0	39.0
10	36.28321042045781	37.0	35.0	39.0	32.0	39.0
11	36.765994636529065	39.0	37.0	39.0	33.0	39.0
12	36.942606072215305	39.0	35.0	39.0	33.0	39.0
13	36.554209366918876	39.0	35.0	39.0	32.0	39.0
14	37.93943348338282	40.0	37.0	41.0	33.0	41.0
15	38.17451632985346	40.0	37.0	41.0	33.0	41.0
16	38.29763672062063	40.0	37.0	41.0	34.0	41.0
17	38.29515850972129	40.0	37.0	41.0	34.0	41.0
18	38.19097787568241	40.0	37.0	41.0	34.0	41.0
19	38.12346757973374	40.0	37.0	41.0	34.0	41.0
20	38.083516904511065	40.0	36.0	41.0	34.0	41.0
21	37.96514941097596	40.0	36.0	41.0	34.0	41.0
22	37.932992529451205	40.0	36.0	41.0	34.0	41.0
23	37.90791111962456	40.0	36.0	41.0	34.0	41.0
24	37.797205727420746	40.0	35.0	41.0	33.0	41.0
25	37.65488219519203	39.0	35.0	41.0	33.0	41.0
26	37.54795996552054	39.0	35.0	41.0	33.0	41.0
27	37.42352265108706	39.0	35.0	41.0	33.0	41.0
28	37.36139977013696	39.0	35.0	41.0	33.0	41.0
29	37.35577291447179	39.0	35.0	41.0	33.0	41.0
30	37.223446030073745	39.0	35.0	41.0	33.0	41.0
31	37.09359735657504	39.0	35.0	41.0	32.0	41.0
32	36.95973805191073	39.0	35.0	41.0	32.0	41.0
33	36.73551383967053	39.0	35.0	41.0	31.0	41.0
34	36.570754716981135	39.0	35.0	41.0	31.0	41.0
35	36.399231395460205	39.0	35.0	41.0	30.0	41.0
36	36.20581122497845	39.0	35.0	41.0	30.0	41.0
37	36.14276649746193	39.0	35.0	41.0	30.0	41.0
38	36.04756488842065	39.0	35.0	40.0	29.0	41.0
39	36.01193611723015	39.0	35.0	41.0	29.0	41.0
40	35.818468058615075	39.0	35.0	40.0	28.0	41.0
41	35.74489991380136	39.0	35.0	40.0	28.0	41.0
42	35.677521310219326	38.0	35.0	40.0	27.0	41.0
43	35.609041279570924	38.0	35.0	40.0	27.0	41.0
44	35.45573939277847	38.0	35.0	40.0	27.0	41.0
45	35.383703668231014	38.0	34.0	40.0	26.0	41.0
46	35.273596877693706	38.0	34.0	40.0	26.0	41.0
47	35.20557178431185	38.0	34.0	40.0	26.0	41.0
48	35.121300641700984	38.0	34.0	40.0	25.0	41.0
49	35.02026865242793	38.0	34.0	40.0	25.0	41.0
50	34.82793793697922	38.0	34.0	40.0	24.0	41.0
51	33.034251987357536	36.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	0.0
9	7.0
10	7.0
11	2.0
12	2.0
13	0.0
14	2.0
15	1.0
16	10.0
17	21.0
18	33.0
19	45.0
20	98.0
21	148.0
22	290.0
23	401.0
24	629.0
25	899.0
26	1082.0
27	1228.0
28	1200.0
29	1415.0
30	1734.0
31	2101.0
32	2880.0
33	4570.0
34	6450.0
35	8202.0
36	8710.0
37	15208.0
38	16021.0
39	10130.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.58346901637774	21.927976247485873	24.64802222009386	14.840532516042524
2	33.81859975098171	24.09611148357437	26.12537113303323	15.959917632410688
3	30.977636241739297	24.543865530121636	28.741260415668997	15.73723781247007
4	28.36294416243655	25.62374293650034	29.42246911215401	16.59084378890911
5	22.209319030744183	37.608945503304284	24.85274399003927	15.328991475912268
6	24.701896370079492	35.476726367206204	27.14419116942822	12.677186093286084
7	46.68614117421703	43.8092615649842	6.63011205823197	2.8744852025668037
8	80.66995498515467	6.303275548319126	9.999042237333589	3.027727229192606
9	74.3882290968298	9.099942534240016	11.742170290202088	4.769658078728091
10	42.78445551192414	29.51465376879609	17.828752035245664	9.872138684034097
11	36.37223446030074	25.53036107652524	23.066516617182263	15.030887845991764
12	35.15587587395843	22.532563930657982	27.07834498611244	15.233215209271142
13	24.42534240015324	29.70740350541136	27.875682405899816	17.991571688535583
14	17.367828752035248	33.90599559429173	28.311464419116945	20.414711234556076
15	17.078105545445837	27.432717172684605	39.21080356287712	16.278373718992434
16	20.332104204578105	24.983239153337802	37.659228043290874	17.02542859879322
17	19.83526482137726	25.205918973278425	30.618475241835075	24.34034096350924
18	21.180921367685087	27.35729336270472	31.020735561727804	20.441049707882385
19	25.78057657312518	27.492577339335313	27.82180825591418	18.905037831625325
20	29.34584809884111	25.031127286658368	28.86696676563548	16.75605784886505
21	24.009912843597355	27.44468920601475	29.970788238674455	18.57460971171344
22	20.890000957762666	26.506081792931713	28.843022698975197	23.760894550330427
23	20.985777224403794	28.19294128914855	30.32755483191265	20.493726654535006
24	21.526913130926157	25.173594483287044	34.302269897519395	18.997222488267408
25	19.729910928072023	28.159419595824154	31.458911981610953	20.651757494492866
26	20.824154774446892	31.63849248156307	28.804712192318743	18.732640551671295
27	20.582319701178047	32.14371228809501	29.757686045397953	17.516281965328993
28	19.60540178143856	28.849008715640263	33.68571018101714	17.85987932190403
29	19.56469686811608	27.061584139450247	33.161335121156974	20.2123838712767
30	21.848960827506943	29.732544775404655	30.440091945215975	17.978402451872427
31	25.197538549947325	27.720045972607988	27.789483765922807	19.292931711521884
32	25.887127669763434	30.701082271813046	26.488123742936498	16.923666315487022
33	23.991954793602147	30.699885068480032	27.29863039938703	18.00952973853079
34	21.206062637678382	29.955224595345275	27.722440379274015	21.116272387702328
35	22.625945790633082	27.309405229384158	29.600852408773104	20.463796571209656
36	24.816827890048845	28.646681352360886	27.13700794943013	19.399482808160137
37	21.159371707690834	31.955751364811803	29.3877502154966	17.497126712000764
38	21.379657120965426	31.461306388276984	27.304616416052102	19.854420074705487
39	23.17426491715353	27.860118762570636	29.048941672253616	19.91667464802222
40	23.266449573795615	27.722440379274015	29.471554448807584	19.539555598122785
41	19.217507901541996	28.48505890240399	30.315582798582515	21.981850397471504
42	21.268317210995118	29.11957666890145	27.229192606072218	22.382913514031223
43	23.64356862369505	28.408437889091083	27.04362608945503	20.904367397758836
44	21.01091849439709	29.9743798486735	29.004645148932095	20.010056507997316
45	20.58830571784312	32.03716119145676	26.145723589694477	21.228809501005653
46	20.10822718130447	32.152092711426114	26.747916866200555	20.991763241068863
47	22.40566037735849	29.20218369887942	28.165405612489224	20.226750311272866
48	22.012977684129872	26.175653673019827	30.677138205152765	21.13423043769754
49	20.20160904127957	27.63863614596303	29.807968585384543	22.35178622737286
50	18.277703285125945	31.51278613159659	29.72536155540657	20.48414902787089
51	20.220764294607797	29.2943683555215	27.620678095967822	22.864189253902882
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	81.0
1	166.5
2	252.0
3	917.0
4	1582.0
5	1080.0
6	578.0
7	516.5
8	455.0
9	450.0
10	445.0
11	404.0
12	363.0
13	344.5
14	326.0
15	333.0
16	340.0
17	338.5
18	337.0
19	330.5
20	324.0
21	390.0
22	456.0
23	609.5
24	763.0
25	870.5
26	1167.5
27	1357.0
28	1636.0
29	1915.0
30	2104.5
31	2294.0
32	2518.5
33	2743.0
34	2682.5
35	2622.0
36	2665.0
37	2708.0
38	3212.5
39	3717.0
40	4519.5
41	5322.0
42	5921.0
43	6520.0
44	6524.5
45	6529.0
46	6539.0
47	6549.0
48	6095.5
49	5642.0
50	5282.5
51	4923.0
52	4646.0
53	4369.0
54	4069.5
55	3770.0
56	3533.0
57	3296.0
58	3345.5
59	3395.0
60	3265.0
61	3135.0
62	2710.5
63	2286.0
64	1801.5
65	1317.0
66	1047.5
67	778.0
68	585.0
69	392.0
70	334.5
71	277.0
72	236.0
73	195.0
74	143.5
75	76.0
76	60.0
77	39.0
78	18.0
79	13.0
80	8.0
81	6.5
82	5.0
83	4.5
84	4.0
85	5.5
86	7.0
87	3.5
88	0.0
89	0.0
90	0.0
91	0.5
92	1.0
93	1.5
94	2.0
95	1.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	83528.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.034479455990805
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.1549043062201	27.285461162723877
2	8.764952153110048	7.018005938128531
3	5.158492822966507	6.19552724834786
4	3.4569377990430623	5.535868211857102
5	2.8588516746411483	5.722631931807298
6	2.08433014354067	5.006704338664878
7	1.8630382775119618	5.221003735274399
8	1.5998803827751196	5.1240302653002585
9	1.2709330143540671	4.5793027487788525
>10	4.736842105263158	25.895508093094534
>50	0.03588516746411483	0.9398046164160521
>100	0.011961722488038277	0.8212814864476583
>500	0.0029904306220095694	0.6548702231587012
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	547	0.6548702231587012	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	317	0.3795134565654631	No Hit
GAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAA	127	0.15204482329278804	No Hit
GAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAA	126	0.15084761995977397	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	116	0.13887558662963317	No Hit
AAGGAAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	86	0.1029594866392108	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.023944066660281583	0.0
2	0.0	0.0	0.0	0.1424671966286754	0.0
3	0.0	0.0	0.0	0.20232736327937936	0.0
4	0.0	0.0	0.0	0.2813427832583086	0.0
5	0.0	0.0	0.0	0.5363470931903075	0.0
6	0.0	0.0	0.0	0.7614213197969543	0.0
7	0.0	0.0	0.0	0.9194521597548128	0.0
8	0.0	0.0	0.0	1.2666411263288957	0.0
9	0.0	0.0	0.0	1.3971362896274304	0.0
10	0.0	0.0	0.0	1.8221434728474284	0.0
11	0.0	0.0	0.0	2.2615170960635953	0.0
12	0.0	0.0	0.0	2.781103342591706	0.0
13	0.0	0.0	0.0	3.0804041758452256	0.0
14	0.0	0.0	0.0	3.251604252466239	0.0
15	0.0	0.0	0.0	3.41921271908821	0.0
16	0.0	0.0	0.0	3.710133129010631	0.0
17	0.0	0.0	0.0	4.059716502250742	0.0
18	0.0	0.0	0.0	4.439229958816205	0.0
19	0.0	0.0	0.0	4.659515372090796	0.0
20	0.0	0.0	0.0	4.839095872042908	0.0
21	0.0	0.0	0.0	5.113255435303132	0.0
22	0.0	0.0	0.0	5.39459821856144	0.0
23	0.0	0.0	0.0	5.66157456182358	0.0
24	0.0	0.0	0.0	5.889043195096255	0.0
25	0.0	0.0	0.0	6.114117421702902	0.0
26	0.0	0.0	0.0	6.244612585001437	0.0
27	0.0	0.0	0.0	6.425390288286563	0.0
28	0.0011972033330140792	0.0	0.0	6.604970788238674	0.0
29	0.0011972033330140792	0.0	0.0	6.816875778182166	0.0
30	0.0011972033330140792	0.0	0.0	7.026386361459631	0.0
31	0.0011972033330140792	0.0	0.0	7.244277368068193	0.0
32	0.0011972033330140792	0.0	0.0	7.460971171343741	0.0
33	0.0011972033330140792	0.0	0.0	7.6525237046259935	0.0
34	0.0011972033330140792	0.0	0.0	7.909922421224021	0.0
35	0.0011972033330140792	0.0	0.0	8.181687577818217	0.0
36	0.0011972033330140792	0.0	0.0	8.422325447754046	0.0
37	0.0011972033330140792	0.0	0.0	8.661766114356862	0.0
38	0.0011972033330140792	0.0	0.0	8.894023560961594	0.0
39	0.0011972033330140792	0.0	0.0	9.103534144239058	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CAGCGGG	25	3.8597056E-5	45.000004	3
ATAGGGC	25	3.8597056E-5	45.000004	5
AATCTAC	25	3.8597056E-5	45.000004	17
CGTTTTA	135	0.0	45.000004	1
GTACGGG	50	2.1827873E-11	45.000004	3
TATCATC	20	6.9957256E-4	45.0	33
GTTTAGG	20	6.9957256E-4	45.0	3
TATGGAG	20	6.9957256E-4	45.0	2
GCTATCA	20	6.9957256E-4	45.0	31
TACGGGA	20	6.9957256E-4	45.0	4
AGACAAG	20	6.9957256E-4	45.0	2
GTTCAGA	20	6.9957256E-4	45.0	1
TAAGGAT	20	6.9957256E-4	45.0	5
AGCTATC	20	6.9957256E-4	45.0	30
GGCGATG	20	6.9957256E-4	45.0	8
CATCCAA	20	6.9957256E-4	45.0	36
TGACTTG	20	6.9957256E-4	45.0	20
CTGTGGG	20	6.9957256E-4	45.0	3
TACCCAT	30	2.1413216E-6	44.999996	17
AAGACGC	30	2.1413216E-6	44.999996	39
>>END_MODULE