##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936802.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	127924
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.922375785622712	31.0	31.0	34.0	30.0	34.0
2	32.11801538413433	33.0	31.0	34.0	30.0	34.0
3	31.86664738438448	33.0	31.0	34.0	30.0	34.0
4	35.71708201744786	37.0	35.0	37.0	35.0	37.0
5	32.09235952596855	37.0	35.0	37.0	10.0	37.0
6	33.96555767486945	37.0	35.0	37.0	19.0	37.0
7	20.646610487476938	32.0	0.0	37.0	0.0	37.0
8	28.032042462712237	35.0	17.0	37.0	17.0	37.0
9	34.80079578499734	37.0	32.0	39.0	32.0	39.0
10	36.22410181044995	37.0	35.0	39.0	32.0	39.0
11	36.7286826553266	38.0	35.0	39.0	33.0	39.0
12	36.98553828835871	39.0	37.0	39.0	33.0	39.0
13	36.62755229667615	39.0	35.0	39.0	33.0	39.0
14	38.00665238735499	40.0	37.0	41.0	33.0	41.0
15	38.21321253244114	40.0	37.0	41.0	33.0	41.0
16	38.32368437509771	40.0	37.0	41.0	34.0	41.0
17	38.31016853756918	40.0	37.0	41.0	34.0	41.0
18	38.200454957631095	40.0	37.0	41.0	34.0	41.0
19	38.131218536005754	40.0	37.0	41.0	34.0	41.0
20	38.08297113911385	40.0	36.0	41.0	34.0	41.0
21	38.001328914042716	40.0	36.0	41.0	34.0	41.0
22	37.928316813107784	40.0	36.0	41.0	34.0	41.0
23	37.940300490916485	40.0	36.0	41.0	34.0	41.0
24	37.81109877739908	40.0	35.0	41.0	33.0	41.0
25	37.69099621650355	39.0	35.0	41.0	33.0	41.0
26	37.53990650698852	39.0	35.0	41.0	33.0	41.0
27	37.44642912979582	39.0	35.0	41.0	33.0	41.0
28	37.41730246083612	39.0	35.0	41.0	33.0	41.0
29	37.379014102123136	39.0	35.0	41.0	33.0	41.0
30	37.14795503580251	39.0	35.0	41.0	32.0	41.0
31	36.99408242393921	39.0	35.0	41.0	31.0	41.0
32	36.864802539007535	39.0	35.0	41.0	31.0	41.0
33	36.584112441762294	39.0	35.0	41.0	30.0	41.0
34	36.442184422000565	39.0	35.0	41.0	30.0	41.0
35	36.1476423501454	39.0	35.0	41.0	29.0	41.0
36	35.946155529845846	39.0	35.0	41.0	28.0	41.0
37	35.81433507395016	39.0	35.0	40.0	27.0	41.0
38	35.60991682561521	39.0	35.0	40.0	26.0	41.0
39	35.589584440761705	39.0	35.0	40.0	26.0	41.0
40	35.402879834901974	39.0	35.0	40.0	24.0	41.0
41	35.27832150339264	38.0	35.0	40.0	24.0	41.0
42	35.21729308026641	38.0	35.0	40.0	24.0	41.0
43	35.10373346674588	38.0	34.0	40.0	23.0	41.0
44	34.959475938838686	38.0	34.0	40.0	23.0	41.0
45	34.851482130014695	38.0	34.0	40.0	23.0	41.0
46	34.636119883680934	38.0	34.0	40.0	23.0	41.0
47	34.609830837059505	38.0	34.0	40.0	22.0	41.0
48	34.49198743003659	38.0	34.0	40.0	22.0	41.0
49	34.37741158813045	38.0	34.0	40.0	21.0	41.0
50	34.24134642443951	38.0	34.0	40.0	20.0	41.0
51	32.33946718364029	36.0	31.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	2.0
9	4.0
10	4.0
11	9.0
12	1.0
13	1.0
14	2.0
15	3.0
16	11.0
17	18.0
18	30.0
19	88.0
20	135.0
21	271.0
22	500.0
23	769.0
24	1171.0
25	1583.0
26	2007.0
27	2129.0
28	2234.0
29	2498.0
30	2901.0
31	3508.0
32	4566.0
33	6852.0
34	9361.0
35	11877.0
36	13096.0
37	22874.0
38	24089.0
39	15328.0
40	2.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.5805321909884	20.39101341421469	25.420562208811482	14.60789218598543
2	32.935180263281325	25.61208217379069	25.759826146774646	15.69291141615334
3	29.515180888652637	24.425440105062382	29.94903223789125	16.110346768393736
4	27.398298990025328	24.719364622744756	29.709827710202934	18.172508677026986
5	21.801225727775865	36.767142991151	24.823332603733466	16.60829867733967
6	24.169037866233076	34.74328507551358	26.876895656796222	14.210781401457115
7	47.0654451080329	43.28351208530064	7.241018104499547	2.4100247021669117
8	81.18961258247084	5.846440073793815	9.815202776648634	3.148744567086708
9	75.62615302836059	7.752259153872612	11.140208248647635	5.481379569119165
10	39.64072417998186	28.855414152152843	18.825239986241833	12.678621681623465
11	32.961758544135584	24.72483662174416	26.20149463744098	16.111910196679275
12	31.323285700884902	22.178793658734875	28.483318220193237	18.014602420186986
13	23.973609330540008	27.578874957005723	30.346924736562336	18.100590975891937
14	18.1607829648854	30.752634376661142	30.446202432694413	20.640380225759046
15	18.422657202714113	26.42819173884494	38.32119070698227	16.827960351458678
16	19.868828366842813	24.901504018010694	36.64597729902129	18.5836903161252
17	19.63744098058222	24.51533723148119	31.297489134173418	24.549732653763172
18	20.718551640036274	27.21694130890216	31.52027766486351	20.544229386198054
19	23.950939620399613	28.50755135861918	29.32287920953066	18.218629811450548
20	25.293924517682377	26.707263687814642	30.038147650167286	17.9606641443357
21	23.17547919076952	27.120790469341173	30.331290453706888	19.37243988618242
22	21.24151840155092	25.886463837903754	29.995935086457585	22.87608267408774
23	19.89227979112598	27.968168600106313	30.24139332728808	21.89815828147963
24	20.223726587661425	25.710578155779995	34.10540633501141	19.960288921547168
25	19.247365623338858	27.87045433225978	31.800913042118754	21.081267002282605
26	19.334917607329352	30.221068759576	29.483912322941748	20.960101310152904
27	19.58193927644539	30.17807448172352	31.2670022826053	18.97298395922579
28	18.375754354147773	28.545855351615025	33.764578968762706	19.3138113254745
29	19.721866108001628	25.633970169788313	33.9224852256027	20.72167849660736
30	19.93292892655014	27.134861323911075	33.356524186235575	19.57568556330321
31	23.99158875582377	26.64316312810731	29.245489509396204	20.11975860667271
32	24.702948625746536	27.875144617116415	28.268346830930863	19.153559926206185
33	23.866514492980208	28.00256402238829	28.73503017416591	19.395891310465586
34	20.715424783465185	27.728964072417998	29.93027109846471	21.625340045652106
35	21.182889840843	25.23842281354554	31.21931771989619	22.35936962571527
36	23.99862418310872	26.350020324567712	30.581438979394015	19.069916512929552
37	21.541696632375473	29.129014102123136	31.387386260592226	17.941903004909165
38	21.59485319408399	28.95156499171383	28.119039429661363	21.33454238454082
39	21.622213189081016	26.85891623151246	31.590631937713017	19.928238641693504
40	22.443794753134675	25.755135861918017	31.189612582470843	20.61145680247647
41	18.454707482567777	26.074075232169104	32.60764203745975	22.863575247803382
42	20.66304993589944	25.865357556048902	30.258591038429067	23.213001469622586
43	22.73850098495982	25.52374847565742	30.302367030424314	21.435383508958445
44	21.170382414558645	27.401425846596418	30.210906475719955	21.217285263124978
45	20.577061380194493	28.83196272786967	28.552109064757197	22.038866827178637
46	21.666770895219038	28.61386448203621	28.888246146149278	20.831118476595478
47	21.026547012288546	26.81670366780276	30.878490353647482	21.27825896626122
48	21.570620055658047	25.248585097401584	31.603139363997375	21.577655482942998
49	20.51530596291548	24.94215315343485	32.694412307307466	21.848128576342205
50	19.65854726243707	27.66799036928176	31.082517744911044	21.590944623370127
51	20.285482004940434	26.221819205153057	29.620712297926893	23.871986491979612
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	150.0
1	239.0
2	328.0
3	1256.5
4	2185.0
5	1496.5
6	808.0
7	724.0
8	640.0
9	613.5
10	587.0
11	573.5
12	560.0
13	530.0
14	500.0
15	493.5
16	487.0
17	473.0
18	459.0
19	506.0
20	553.0
21	647.0
22	741.0
23	771.0
24	801.0
25	919.5
26	1339.5
27	1641.0
28	2022.0
29	2403.0
30	2712.0
31	3021.0
32	3310.0
33	3599.0
34	4006.0
35	4413.0
36	4707.5
37	5002.0
38	5634.5
39	6267.0
40	7008.0
41	7749.0
42	8612.0
43	9475.0
44	10127.0
45	10779.0
46	11235.0
47	11691.0
48	10876.5
49	10062.0
50	9478.5
51	8895.0
52	8157.5
53	7420.0
54	6563.5
55	5707.0
56	5284.5
57	4862.0
58	4616.5
59	4371.0
60	4001.5
61	3632.0
62	3234.5
63	2837.0
64	2305.0
65	1773.0
66	1450.5
67	1128.0
68	841.0
69	554.0
70	446.5
71	339.0
72	275.5
73	212.0
74	163.5
75	90.5
76	66.0
77	54.0
78	42.0
79	28.5
80	15.0
81	12.0
82	9.0
83	7.5
84	6.0
85	3.0
86	0.0
87	1.0
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	127924.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	40.68587598886839
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.06179030491671	29.3189706388168
2	7.746844198512883	6.303742847315593
3	4.088612215881799	4.990463087458178
4	2.734067285338252	4.449516900659766
5	2.190327972793821	4.4557706138019455
6	1.6946221684246932	4.1368312435508585
7	1.4967241147424444	4.2626872205371935
8	1.464061329183238	4.765329414339764
9	1.0586585201836802	3.876520434007692
>10	5.425865083482237	31.243550858322127
>50	0.023056083924145484	0.6980707294956381
>100	0.013449382289084866	0.9693255370376161
>500	0.0019213403270121237	0.5292204746568275
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	677	0.5292204746568275	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	415	0.32441136925049247	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	181	0.14149025984178104	No Hit
AAGGAAGGAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAGAAAG	151	0.11803883555861291	No Hit
GAATCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCGTCTTC	138	0.1078765517025734	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTGGTTCTTCGTATGCCG	128	0.1000594102748507	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	7.817141427722711E-4	0.0	0.0	0.04533942028079172	0.0
2	7.817141427722711E-4	0.0	0.0	0.25171195397267127	0.0
3	7.817141427722711E-4	0.0	0.0	0.30643194396673024	0.0
4	7.817141427722711E-4	0.0	0.0	0.4346330633813827	0.0
5	7.817141427722711E-4	0.0	0.0	0.8833369813326662	0.0
6	7.817141427722711E-4	0.0	0.0	1.1545917888746444	0.0
7	7.817141427722711E-4	0.0	0.0	1.4235014539883055	0.0
8	7.817141427722711E-4	0.0	0.0	1.990244207498202	0.0
9	7.817141427722711E-4	0.0	0.0	2.1856727431912697	0.0
10	7.817141427722711E-4	0.0	0.0	3.072136581095025	0.0
11	7.817141427722711E-4	0.0	0.0	3.847597010725118	0.0
12	7.817141427722711E-4	0.0	0.0	4.823957975047684	0.0
13	7.817141427722711E-4	0.0	0.0	5.257027610143523	0.0
14	7.817141427722711E-4	0.0	0.0	5.480597854976392	0.0
15	7.817141427722711E-4	0.0	0.0	5.697132672524312	0.0
16	7.817141427722711E-4	0.0	0.0	6.083299459053813	0.0
17	7.817141427722711E-4	0.0	0.0	6.56405365685876	0.0
18	7.817141427722711E-4	0.0	0.0	7.094055845658359	0.0
19	7.817141427722711E-4	0.0	0.0	7.377818079484694	0.0
20	7.817141427722711E-4	0.0	0.0	7.632656890028454	0.0
21	7.817141427722711E-4	0.0	0.0	8.008661392701917	0.0
22	7.817141427722711E-4	0.0	0.0	8.401081892373597	0.0
23	7.817141427722711E-4	0.0	0.0	8.79819267690191	0.0
24	7.817141427722711E-4	0.0	0.0	9.110096619868047	0.0
25	7.817141427722711E-4	0.0	0.0	9.3594634314124	0.0
26	7.817141427722711E-4	0.0	0.0	9.604139958100122	0.0
27	7.817141427722711E-4	0.0	0.0	9.836309058503486	0.0
28	7.817141427722711E-4	0.0	0.0	10.102091867046058	0.0
29	7.817141427722711E-4	0.0	0.0	10.376473531159126	0.0
30	7.817141427722711E-4	0.0	0.0	10.699321472124074	0.0
31	7.817141427722711E-4	0.0	0.0	10.979956849379318	0.0
32	7.817141427722711E-4	0.0	0.0	11.26215565492011	0.0
33	7.817141427722711E-4	0.0	0.0	11.537319033175947	0.0
34	7.817141427722711E-4	0.0	0.0	11.805446984146837	0.0
35	7.817141427722711E-4	0.0	0.0	12.10562521497139	0.0
36	7.817141427722711E-4	0.0	0.0	12.418310872080298	0.0
37	7.817141427722711E-4	0.0	0.0	12.680185109909008	0.0
38	7.817141427722711E-4	0.0	0.0	12.943622776023263	0.0
39	7.817141427722711E-4	0.0	0.0	13.22113129670742	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGCCGGT	30	2.1503492E-6	45.000004	9
ACTAGGA	30	2.1503492E-6	45.000004	4
GGGCCGG	30	2.1503492E-6	45.000004	8
CCCATTA	30	2.1503492E-6	45.000004	33
GGTACGG	20	7.0097705E-4	45.0	2
CACGGGT	20	7.0097705E-4	45.0	4
TAGACGG	20	7.0097705E-4	45.0	2
TTTCGTT	20	7.0097705E-4	45.0	44
ATTGTAC	20	7.0097705E-4	45.0	19
TTCGTCG	20	7.0097705E-4	45.0	40
TAACGGG	35	1.2006421E-7	45.0	3
AGGCGAT	25	3.8713275E-5	45.0	7
GTGGCGG	25	3.8713275E-5	45.0	14
ACGGGTT	20	7.0097705E-4	45.0	5
ACGGGAA	20	7.0097705E-4	45.0	5
GCACCAC	20	7.0097705E-4	45.0	29
TCGTGTA	20	7.0097705E-4	45.0	17
TCTACGG	20	7.0097705E-4	45.0	2
GTTCGTC	20	7.0097705E-4	45.0	39
CACTACT	20	7.0097705E-4	45.0	21
>>END_MODULE