##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936801.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	67643
Sequences flagged as poor quality	0
Sequence length	51
%GC	42
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.781322531525806	31.0	31.0	34.0	30.0	34.0
2	31.94806557958695	33.0	31.0	34.0	30.0	34.0
3	31.55782564345165	33.0	31.0	34.0	28.0	34.0
4	35.495513209053414	37.0	35.0	37.0	33.0	37.0
5	31.82181452626288	37.0	35.0	37.0	0.0	37.0
6	33.77305855742649	37.0	35.0	37.0	17.0	37.0
7	20.959862809159855	32.0	0.0	37.0	0.0	37.0
8	28.188415652765254	35.0	17.0	37.0	17.0	37.0
9	34.870940082491906	37.0	32.0	39.0	32.0	39.0
10	36.29955797347841	37.0	35.0	39.0	32.0	39.0
11	36.56304421743565	38.0	35.0	39.0	32.0	39.0
12	36.72647576245879	39.0	35.0	39.0	33.0	39.0
13	36.225936164865544	38.0	35.0	39.0	32.0	39.0
14	37.61317505137265	40.0	36.0	41.0	33.0	41.0
15	37.93295684697603	40.0	36.0	41.0	33.0	41.0
16	38.073991396005496	40.0	36.0	41.0	34.0	41.0
17	38.0309714235028	40.0	36.0	41.0	34.0	41.0
18	37.911077273332054	40.0	36.0	41.0	33.0	41.0
19	37.80228552843605	39.0	36.0	41.0	34.0	41.0
20	37.7029404372958	39.0	35.0	41.0	34.0	41.0
21	37.59898289549547	39.0	35.0	41.0	33.0	41.0
22	37.57649719852756	39.0	35.0	41.0	33.0	41.0
23	37.528436053989324	39.0	35.0	41.0	33.0	41.0
24	37.34903833360436	39.0	35.0	41.0	33.0	41.0
25	37.25683367088982	39.0	35.0	41.0	33.0	41.0
26	37.13384977011664	39.0	35.0	41.0	33.0	41.0
27	37.09170202386056	39.0	35.0	40.0	33.0	41.0
28	37.05725647886699	39.0	35.0	41.0	33.0	41.0
29	37.099596410567244	39.0	35.0	41.0	33.0	41.0
30	36.947252487323155	39.0	35.0	40.0	33.0	41.0
31	36.784530550093876	39.0	35.0	40.0	32.0	41.0
32	36.5585056842541	38.0	35.0	40.0	31.0	41.0
33	36.273272918114216	38.0	35.0	40.0	30.0	41.0
34	36.03987108791745	38.0	35.0	40.0	30.0	41.0
35	35.76804695238236	38.0	35.0	40.0	29.0	41.0
36	35.54055852046775	38.0	35.0	40.0	27.0	41.0
37	35.34083349348787	38.0	35.0	40.0	25.0	41.0
38	35.23693508567036	38.0	35.0	40.0	25.0	41.0
39	35.218426149047204	38.0	35.0	40.0	25.0	41.0
40	35.05829132356637	38.0	35.0	40.0	24.0	41.0
41	35.00342977100365	38.0	34.0	40.0	24.0	41.0
42	34.90427686530757	38.0	34.0	40.0	23.0	41.0
43	34.753411291634016	38.0	34.0	40.0	23.0	41.0
44	34.58322368907352	38.0	34.0	40.0	23.0	41.0
45	34.52614461215499	38.0	34.0	40.0	23.0	41.0
46	34.358499770855815	38.0	34.0	40.0	22.0	41.0
47	34.30786629806484	38.0	33.0	40.0	22.0	41.0
48	34.20887601082152	38.0	33.0	40.0	20.0	41.0
49	34.11161539257573	37.0	34.0	40.0	20.0	41.0
50	33.91882382508168	37.0	33.0	40.0	20.0	41.0
51	32.1852519846843	35.0	31.0	39.0	13.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	1.0
9	4.0
10	1.0
11	1.0
12	3.0
13	2.0
14	0.0
15	1.0
16	2.0
17	12.0
18	18.0
19	35.0
20	75.0
21	142.0
22	299.0
23	460.0
24	743.0
25	1017.0
26	1247.0
27	1233.0
28	1310.0
29	1327.0
30	1551.0
31	1892.0
32	2657.0
33	4004.0
34	5610.0
35	6953.0
36	6822.0
37	11408.0
38	11808.0
39	7005.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	36.90699702851737	18.813476634685035	26.248096624927932	18.031429711869666
2	33.10024688437828	25.004804636104254	26.01303904321216	15.881909436305309
3	28.91799594932218	23.811776532678916	32.88145114793844	14.388776370060462
4	25.729195925668584	24.091184601510875	33.00563251186375	17.17398696095679
5	22.262466182753574	35.6681400884053	26.981358011915496	15.088035716925624
6	24.543559570095944	32.34333190426208	29.447245095575298	13.665863430066674
7	46.28121165530801	42.208358588471825	9.16133228863297	2.3490974675871854
8	80.03932409857634	5.254054373697204	11.93028103425336	2.7763404934730866
9	75.41800334107003	7.2276510503673705	12.6251053324069	4.7292402761557
10	44.49240867495528	25.503008441376046	19.356030927073014	10.648551956595655
11	35.44786600239493	21.960882870363527	29.17670712416658	13.414544003074965
12	32.77353162928906	21.143355557855212	30.907854471268276	15.175258341587453
13	20.223822124979673	31.96487441420398	32.20880209334299	15.602501367473353
14	15.723726032257588	34.31692858093225	33.57184039738036	16.3875049894298
15	15.164909894593675	25.217686974261934	44.98322073237438	14.634182398770012
16	17.821504072853067	22.65570716851707	41.17351388909422	18.349274869535652
17	18.05360495542776	22.510828910604204	33.9029907011812	25.532575432786835
18	21.045784486199608	25.65527844714161	34.05378235737623	19.24515470928256
19	23.875345564212115	27.722011146755758	30.328341439616814	18.074301849415313
20	26.163830699407182	25.60205786260219	32.662655411498605	15.571456026492026
21	21.49815945478468	27.62296172552962	32.69517910205047	18.183699717635232
22	20.543145632216195	23.016424463728693	32.664133761069145	23.77629614298597
23	18.562157207693332	27.40416598908978	32.26941442573511	21.76426237748178
24	19.805449196517007	24.199104120160253	37.524947149002855	18.470499534319885
25	17.309995121446416	27.12919296896944	34.99992608252147	20.560885827062666
26	16.393418387711957	32.2428041334654	31.602678769421814	19.761098709400827
27	16.023830995077095	31.796342563162483	34.296231686944694	17.883594754815725
28	15.262480966249278	27.93637183448398	39.73951480567095	17.06163239359579
29	16.099226823174607	23.292875833419572	39.026950312670934	21.580947030734887
30	15.448753012137251	30.80437000133052	35.11819404816463	18.628682938367604
31	23.93743624617477	26.549679937317975	30.93150806439691	18.581375752110343
32	23.056339902133256	28.362136510799346	30.80880505004213	17.772718537025263
33	21.224664784234882	29.568469760359534	29.849356178762033	19.357509276643555
34	18.88887246278255	27.846192510681078	32.44977307334092	20.815161953195453
35	17.91464009579705	28.067944946261992	32.233934036042164	21.783480921898793
36	23.66837662433659	27.234155788477743	31.141433703413508	17.956033883772157
37	18.501544875301214	29.639430539745426	33.975429830137635	17.883594754815725
38	18.2650089440149	30.43921765740727	29.22401431042384	22.071759088153986
39	19.32646393566223	28.805641381961177	33.09581183566666	18.772082846709935
40	20.68802389012906	28.279348934849136	30.91820291826205	20.11442425675975
41	17.150333367828157	27.70279260233875	31.898348683529708	23.248525346303385
42	21.480419259938206	25.724760876956964	29.861182975326344	22.933636887778484
43	21.54694499061248	26.904483834247443	28.288219032272373	23.260352142867703
44	18.69668701861242	28.919474298892716	29.818310837780704	22.56552784471416
45	17.40904454267256	33.09581183566666	28.130035628224647	21.365107993436126
46	21.236491580799196	31.86582499297784	27.88610794908564	19.011575477137324
47	18.65233653149624	28.564670401963248	30.669840190411424	22.11315287612909
48	20.53131883565188	26.67386130124329	32.07279393285336	20.722025930251466
49	20.203125230992118	23.66394157562497	33.50235796756501	22.630575225817896
50	17.104504531141433	29.86857472317904	32.29011131972266	20.736809425956864
51	17.147376668687077	30.17459308428071	29.141226734473634	23.536803512558578
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	67.0
1	113.5
2	160.0
3	913.0
4	1666.0
5	1173.0
6	680.0
7	612.5
8	545.0
9	535.5
10	526.0
11	509.0
12	492.0
13	492.5
14	493.0
15	492.5
16	492.0
17	479.0
18	466.0
19	415.0
20	364.0
21	385.5
22	407.0
23	428.0
24	449.0
25	537.5
26	710.0
27	794.0
28	962.5
29	1131.0
30	1257.0
31	1383.0
32	1592.0
33	1801.0
34	2155.0
35	2509.0
36	2740.5
37	2972.0
38	3195.5
39	3419.0
40	4085.5
41	4752.0
42	5303.0
43	5854.0
44	6169.5
45	6485.0
46	6564.5
47	6644.0
48	5829.5
49	5015.0
50	4501.0
51	3987.0
52	3516.5
53	3046.0
54	2835.0
55	2624.0
56	2332.5
57	2041.0
58	1897.5
59	1754.0
60	1655.0
61	1556.0
62	1342.0
63	1128.0
64	827.5
65	527.0
66	424.0
67	321.0
68	287.0
69	253.0
70	177.0
71	101.0
72	74.0
73	47.0
74	40.5
75	25.5
76	17.0
77	13.0
78	9.0
79	6.5
80	4.0
81	2.5
82	1.0
83	0.5
84	0.0
85	0.0
86	0.0
87	0.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	67643.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.156823322442825
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.68509156650708	32.536995698002755
2	7.730422846429407	6.82701831675118
3	3.933844454116308	5.2111822361515605
4	2.6750142287990895	4.724805227444081
5	2.1761692724898722	4.804636104253212
6	1.8045465198031403	4.780982511124581
7	1.6103652616425057	4.977603004006327
8	1.2521343198634036	4.423221915054033
9	1.0010378653453413	3.978238694321659
>10	4.094546185007868	24.944192303712136
>50	0.013391810907629984	0.45385331815561103
>100	0.020087716361444977	1.4073887911535563
>500	0.003347952726907496	0.929881879869314
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	629	0.929881879869314	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	329	0.486377008707479	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCG	148	0.21879573643983855	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTC	123	0.18183699717635232	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	121	0.17888029803527342	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGC	120	0.17740194846473398	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	111	0.16409680232987892	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCG	97	0.1433999083423266	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCGCCTATAGTCGTATGC	82	0.12122466478423487	No Hit
GAATCTATCTCTTATACACATCTGACGCGCCTATAGTCGTATGCCGTCTTC	71	0.10496281950830094	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.034002040122407345	0.0
2	0.0	0.0	0.0	0.2350575817157725	0.0
3	0.0	0.0	0.0	0.28088641840249545	0.0
4	0.0	0.0	0.0	0.4242863267448221	0.0
5	0.0	0.0	0.0	0.8855313927531304	0.0
6	0.0	0.0	0.0	1.068846739500022	0.0
7	0.0	0.0	0.0	1.3452981091908993	0.0
8	0.0	0.0	0.0	1.8878524015788773	0.0
9	0.0	0.0	0.0	2.031252309921204	0.0
10	0.0	0.0	0.0	2.9271321496681106	0.0
11	0.0	0.0	0.0	3.521428677024969	0.0
12	0.0	0.0	0.0	4.3241724938278905	0.0
13	0.0	0.0	0.0	4.544446579838269	0.0
14	0.0	0.0	0.0	4.633147554070636	0.0
15	0.0	0.0	0.0	4.798722705971054	0.0
16	0.0	0.0	0.0	5.134308058483509	0.0
17	0.0	0.0	0.0	5.5364191416702395	0.0
18	0.0	0.0	0.0	6.064189938352823	0.0
19	0.0	0.0	0.0	6.356903153319634	0.0
20	0.0	0.0	0.0	6.606744230740801	0.0
21	0.0	0.0	0.0	6.948242981535413	0.0
22	0.0	0.0	0.0	7.334092219446211	0.0
23	0.0	0.0	0.0	7.779075440178585	0.0
24	0.0	0.0	0.0	8.080658752568633	0.0
25	0.0	0.0	0.0	8.334934878701418	0.0
26	0.0	0.0	0.0	8.602516150969057	0.0
27	0.0	0.0	0.0	8.887837618083172	0.0
28	0.0	0.0	0.0	9.174637434767826	0.0
29	0.0	0.0	0.0	9.476220747157873	0.0
30	0.0	0.0	0.0	9.798500953535473	0.0
31	0.0	0.0	0.0	10.160696598317639	0.0
32	0.0	0.0	0.0	10.419407773162042	0.0
33	0.0	0.0	0.0	10.670727200153749	0.0
34	0.0	0.0	0.0	11.000399154384045	0.0
35	0.0	0.0	0.0	11.39068344100646	0.0
36	0.0	0.0	0.0	11.724790443948375	0.0
37	0.0	0.0	0.0	12.002720163209792	0.0
38	0.0	0.0	0.0	12.333870467010629	0.0
39	0.0	0.0	0.0	12.638410478541756	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCCTCCG	30	2.1352225E-6	45.000004	44
CAGCACA	30	2.1352225E-6	45.000004	45
TACGGGC	30	2.1352225E-6	45.000004	4
CAACAAG	35	1.1894008E-7	45.000004	2
GTATAGG	30	2.1352225E-6	45.000004	3
GTTTAGG	35	1.1894008E-7	45.000004	3
CCTCCGC	30	2.1352225E-6	45.000004	45
ACGGCTG	35	1.1894008E-7	45.000004	8
TCTTATT	30	2.1352225E-6	45.000004	11
TAGGCGA	30	2.1352225E-6	45.000004	6
CGTTATT	75	0.0	45.0	1
ATAAGTG	20	6.9862226E-4	45.0	31
GGCAATT	20	6.9862226E-4	45.0	10
TAAGTGC	20	6.9862226E-4	45.0	32
AGCTATA	20	6.9862226E-4	45.0	25
TACGGCT	20	6.9862226E-4	45.0	7
TTTCACT	20	6.9862226E-4	45.0	44
GAATGAA	20	6.9862226E-4	45.0	1
AAAAGTC	20	6.9862226E-4	45.0	34
TTGGGCA	20	6.9862226E-4	45.0	5
>>END_MODULE