##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936800.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	128105
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.98978962569767	33.0	31.0	34.0	30.0	34.0
2	32.18155419382538	33.0	31.0	34.0	30.0	34.0
3	31.967596893173567	33.0	31.0	34.0	30.0	34.0
4	35.76880683814059	37.0	35.0	37.0	35.0	37.0
5	31.999765817103157	37.0	35.0	37.0	0.0	37.0
6	33.927348659302915	37.0	35.0	37.0	18.0	37.0
7	20.79867296358456	32.0	0.0	37.0	0.0	37.0
8	28.109332188439172	35.0	17.0	37.0	17.0	37.0
9	34.84180945318293	37.0	32.0	39.0	32.0	39.0
10	36.30294680145193	37.0	35.0	39.0	32.0	39.0
11	36.718691698216304	38.0	35.0	39.0	33.0	39.0
12	36.99813434292182	39.0	37.0	39.0	34.0	39.0
13	36.726099683853086	39.0	35.0	39.0	33.0	39.0
14	38.08811521798525	40.0	37.0	41.0	33.0	41.0
15	38.32604504117716	40.0	38.0	41.0	34.0	41.0
16	38.43034229733422	40.0	38.0	41.0	34.0	41.0
17	38.43703212208735	40.0	38.0	41.0	34.0	41.0
18	38.30226767105109	40.0	37.0	41.0	34.0	41.0
19	38.24194996292104	40.0	37.0	41.0	34.0	41.0
20	38.213481128761565	40.0	37.0	41.0	34.0	41.0
21	38.12144725030249	40.0	37.0	41.0	34.0	41.0
22	38.09466453300028	40.0	37.0	41.0	34.0	41.0
23	38.05084110690449	40.0	36.0	41.0	34.0	41.0
24	37.912407790484366	40.0	36.0	41.0	34.0	41.0
25	37.795433433511576	40.0	36.0	41.0	33.0	41.0
26	37.66709339994536	40.0	36.0	41.0	33.0	41.0
27	37.63692283673549	40.0	36.0	41.0	33.0	41.0
28	37.59388782639241	40.0	36.0	41.0	33.0	41.0
29	37.57278794738691	40.0	36.0	41.0	33.0	41.0
30	37.43610319659654	40.0	36.0	41.0	33.0	41.0
31	37.343733655985325	40.0	35.0	41.0	33.0	41.0
32	37.18972717692518	39.0	35.0	41.0	32.0	41.0
33	37.04123960813395	40.0	35.0	41.0	32.0	41.0
34	36.86223800788416	40.0	35.0	41.0	31.0	41.0
35	36.71695093868311	40.0	35.0	41.0	31.0	41.0
36	36.566410366496235	39.0	35.0	41.0	30.0	41.0
37	36.47819366925569	39.0	35.0	41.0	30.0	41.0
38	36.33934662971781	39.0	35.0	41.0	30.0	41.0
39	36.29629600718161	39.0	35.0	41.0	30.0	41.0
40	36.1648179227977	39.0	35.0	41.0	29.0	41.0
41	36.05148120682253	39.0	35.0	40.0	29.0	41.0
42	35.92965145778853	39.0	35.0	40.0	28.0	41.0
43	35.86358065649272	39.0	35.0	40.0	28.0	41.0
44	35.730775535693375	39.0	35.0	40.0	27.0	41.0
45	35.69549197923578	39.0	35.0	40.0	27.0	41.0
46	35.520939854026	38.0	35.0	40.0	26.0	41.0
47	35.521720463682136	38.0	35.0	40.0	26.0	41.0
48	35.437305335467	38.0	35.0	40.0	26.0	41.0
49	35.31827016900199	38.0	34.0	40.0	26.0	41.0
50	35.12378127317435	38.0	34.0	40.0	25.0	41.0
51	33.40973420241208	36.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	8.0
10	4.0
11	7.0
12	5.0
13	5.0
14	2.0
15	5.0
16	13.0
17	22.0
18	54.0
19	62.0
20	125.0
21	218.0
22	343.0
23	567.0
24	931.0
25	1296.0
26	1590.0
27	1729.0
28	1828.0
29	1999.0
30	2432.0
31	3127.0
32	4196.0
33	6341.0
34	8832.0
35	11416.0
36	13779.0
37	24000.0
38	26179.0
39	16986.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.98590999570665	20.49646774130596	24.811677920455878	13.705944342531517
2	34.78943054525585	24.608719409859102	25.011513992428085	15.59033605245697
3	31.43671207212833	23.504937356075096	28.77951680262285	16.278833769173726
4	27.881035088404044	24.828851332890988	29.219780648686623	18.070332930018342
5	22.366808477420864	36.32332851957379	24.310526521213067	16.99933648179228
6	25.817103157566056	34.91901174817533	26.10905116896296	13.154833925295655
7	47.70149486749151	43.16068849771671	6.439249053510792	2.6985675812809804
8	81.83989695952539	5.799929745130948	9.631942547129308	2.7282307482143553
9	75.94161039772061	7.632801217751063	11.364895983763319	5.060692400764998
10	41.6384996682409	28.178447367393932	18.17571523359744	12.007337730767729
11	32.55766753834745	25.948245579797824	26.009913742632996	15.484173139221733
12	31.27902892158776	23.377697982124037	28.86694508411069	16.47632801217751
13	24.5767144139573	26.594590375082937	30.181491745052885	18.647203465906873
14	17.82522149798993	30.41099098395847	31.00269310331369	20.76109441473791
15	17.854104055267165	28.692088521135005	35.77534054096249	17.678466882635337
16	21.664259786893563	26.639865735139146	34.83548651496819	16.860387962999102
17	21.91639670582725	26.133250068303344	30.61941376214824	21.330939463721165
18	23.554896374068147	26.52511611568635	30.595995472463994	19.323992037781508
19	24.398735412357052	28.272120526130905	28.59763475274189	18.73150930877015
20	27.31275125873307	26.64767183170056	29.220561258342766	16.819015651223605
21	24.53300027321338	26.94118106240974	30.65688302564303	17.868935638733852
22	23.314468599976582	24.304281643963936	30.504664142695447	21.87658561336404
23	22.20209983997502	27.222981148276805	30.761484719565978	19.813434292182194
24	22.032707544592327	24.66804574372585	33.44834315600484	19.850903555676982
25	20.80246672651341	27.9989071464814	30.62409742008509	20.574528706920102
26	20.72908941883611	30.67952070567113	29.46489208071504	19.126497794777723
27	19.6214043167714	31.044065415089182	30.95741774325748	18.377112524881934
28	19.640138948518793	28.621053042426137	32.938605050544474	18.8002029585106
29	19.772061980406697	27.308848210452364	33.154833925295655	19.764255883845284
30	20.932047929432887	28.45166074704344	31.88946567269037	18.7268256508333
31	24.534561492525665	27.018461418367746	29.50860622145896	18.938370867647635
32	23.985012294602086	28.283049061316888	29.685024003746925	18.0469146403341
33	24.429179188946566	27.763943639982823	28.55470122165411	19.252175949416493
34	21.14983802349635	27.430623316810426	30.419577690176027	20.999960969517193
35	21.345771047187853	28.134733226650017	30.208032473361694	20.311463252800436
36	23.090433628663988	28.59295109480504	28.80293509230709	19.513680184223876
37	21.00854767573475	29.787283868701458	29.837242886694508	19.366925568869288
38	21.135787049685806	29.57027438429413	28.33300807930994	20.96093048671012
39	21.00854767573475	29.069122985051326	29.151867608602316	20.770461730611608
40	23.935833886265172	26.30654541196675	30.01053823035791	19.74708247141017
41	19.68151126029429	27.3150930877015	31.282151360212325	21.721244291791887
42	22.468287732719254	27.4696537996175	28.414191483548652	21.647866984114593
43	22.291869950431288	27.979391905077865	28.155029077709692	21.573709066781156
44	20.00078060965614	29.673314858904803	29.661605714062684	20.66429881737637
45	20.587018461418367	30.115139924280864	27.905233987744428	21.39260762655634
46	20.961711096366262	30.379766597712816	28.34003356621521	20.31848873970571
47	20.730650638148393	28.534405370594435	29.424300378595685	21.31064361266149
48	20.75016587955193	26.926349478943056	31.352406229265057	20.97107841223996
49	21.086608641348896	27.49151086998946	30.041762616603567	21.380117872058076
50	19.306818625346395	30.061277858007102	29.916865071620936	20.715038445025566
51	19.581593224308186	29.08395456851801	28.460247453260994	22.874204753912807
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	94.0
1	240.5
2	387.0
3	1163.5
4	1940.0
5	1349.5
6	759.0
7	686.0
8	613.0
9	590.5
10	568.0
11	556.0
12	544.0
13	543.0
14	542.0
15	528.5
16	515.0
17	548.5
18	582.0
19	576.0
20	570.0
21	585.0
22	600.0
23	741.5
24	883.0
25	1111.0
26	1591.5
27	1844.0
28	2163.5
29	2483.0
30	2869.5
31	3256.0
32	3660.5
33	4065.0
34	4556.5
35	5048.0
36	5422.0
37	5796.0
38	6242.0
39	6688.0
40	7411.0
41	8134.0
42	8693.5
43	9253.0
44	9437.0
45	9621.0
46	9539.5
47	9458.0
48	9210.0
49	8962.0
50	8599.5
51	8237.0
52	7478.0
53	6719.0
54	6383.5
55	6048.0
56	5682.5
57	5317.0
58	5019.5
59	4722.0
60	4548.0
61	4374.0
62	3836.0
63	3298.0
64	2647.0
65	1996.0
66	1617.5
67	1239.0
68	995.5
69	752.0
70	565.0
71	378.0
72	308.0
73	238.0
74	178.5
75	84.5
76	50.0
77	43.5
78	37.0
79	25.0
80	13.0
81	12.5
82	12.0
83	8.5
84	5.0
85	5.0
86	5.0
87	3.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	128105.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	41.05928730338395
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.26743854445903	27.619530853596657
2	9.372801764292097	7.696811209554662
3	5.1635962660887085	6.360407478240505
4	3.67687598623548	6.03879629991023
5	2.893591132911272	5.940439483236408
6	2.3764710355710186	5.8545724210608485
7	1.9030780052852716	5.469731860583115
8	1.701553261468849	5.589165137972757
9	1.3004049506644613	4.805433043206745
>10	4.325177284739254	23.408141758713555
>50	0.013308237799197705	0.30756020451972993
>100	0.0038023536569136295	0.39733031497599625
>500	0.0019011768284568147	0.5120799344287889
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	656	0.5120799344287889	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	373	0.2911674017407595	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	136	0.10616291323523673	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.025760118652667734	0.0
2	0.0	0.0	0.0	0.11474961945279263	0.0
3	0.0	0.0	0.0	0.15846376019671363	0.0
4	0.0	0.0	0.0	0.22169314234417078	0.0
5	0.0	0.0	0.0	0.40357519222512783	0.0
6	0.0	0.0	0.0	0.5237890792709106	0.0
7	0.0	0.0	0.0	0.6486866242535421	0.0
8	0.0	0.0	0.0	0.9343897584013114	0.0
9	0.0	0.0	0.0	1.0186956012645876	0.0
10	0.0	0.0	0.0	1.3418679989071465	0.0
11	0.0	0.0	0.0	1.727489169041021	0.0
12	0.0	0.0	0.0	2.0904726591467937	0.0
13	0.0	0.0	0.0	2.249717028999649	0.0
14	0.0	0.0	0.0	2.3207525077085203	0.0
15	0.0	0.0	0.0	2.442527614066586	0.0
16	0.0	0.0	0.0	2.686858436438859	0.0
17	0.0	0.0	0.0	2.947582061590102	0.0
18	0.0	0.0	0.0	3.2442137309238515	0.0
19	0.0	0.0	0.0	3.449514070489052	0.0
20	0.0	0.0	0.0	3.6743296514577883	0.0
21	0.0	0.0	0.0	3.896022793801959	0.0
22	0.0	0.0	0.0	4.12318020373912	0.0
23	0.0	0.0	0.0	4.3721946840482415	0.0
24	0.0	0.0	0.0	4.568908317395886	0.0
25	0.0	0.0	0.0	4.74922914796456	0.0
26	0.0	0.0	0.0	4.925646930252527	0.0
27	0.0	0.0	0.0	5.123921782912455	0.0
28	0.0	0.0	0.0	5.280043714140744	0.0
29	0.0	0.0	0.0	5.468951250926974	0.0
30	0.0	0.0	0.0	5.703914757425549	0.0
31	0.0	0.0	0.0	5.922485461145154	0.0
32	0.0	0.0	0.0	6.109831778619101	0.0
33	0.0	0.0	0.0	6.31435150852816	0.0
34	0.0	0.0	0.0	6.5173100191249365	0.0
35	0.0	0.0	0.0	6.750712306311229	0.0
36	0.0	0.0	0.0	6.957573865188713	0.0
37	0.0	0.0	0.0	7.1987822489364195	0.0
38	0.0	0.0	0.0	7.443893680964834	0.0
39	0.0	0.0	0.0	7.7444283985792906	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGG	25	3.8713602E-5	45.000004	3
CACGGGC	25	3.8713602E-5	45.000004	4
TGGGTCT	25	3.8713602E-5	45.000004	6
GCGTAGG	25	3.8713602E-5	45.000004	3
GACCGAT	35	1.2006603E-7	45.0	9
GATCAAT	20	7.009812E-4	45.0	35
CGCGAGG	20	7.009812E-4	45.0	3
ATCAATT	20	7.009812E-4	45.0	36
TATGAGG	20	7.009812E-4	45.0	3
TCAATTG	20	7.009812E-4	45.0	37
AATTGTC	20	7.009812E-4	45.0	39
CTCGCTT	20	7.009812E-4	45.0	41
TTCGGGA	20	7.009812E-4	45.0	4
GCTACGG	20	7.009812E-4	45.0	2
TCGACGT	20	7.009812E-4	45.0	26
ACGACGG	20	7.009812E-4	45.0	2
TTGGGCG	20	7.009812E-4	45.0	5
TGCGTAG	20	7.009812E-4	45.0	2
TGAGGAT	30	2.1503765E-6	44.999996	5
CGTTTTA	140	0.0	43.392857	1
>>END_MODULE