##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936799.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	74250
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.89830303030303	31.0	31.0	34.0	30.0	34.0
2	32.07313131313131	33.0	31.0	34.0	30.0	34.0
3	31.899461279461278	33.0	31.0	34.0	30.0	34.0
4	35.68608754208754	37.0	35.0	37.0	35.0	37.0
5	31.999474747474746	37.0	35.0	37.0	0.0	37.0
6	33.886383838383836	37.0	35.0	37.0	19.0	37.0
7	20.711973063973065	32.0	0.0	37.0	0.0	37.0
8	27.954047138047137	35.0	17.0	37.0	17.0	37.0
9	34.54183164983165	35.0	32.0	39.0	28.0	39.0
10	36.14051178451179	37.0	35.0	39.0	32.0	39.0
11	36.614612794612796	38.0	35.0	39.0	33.0	39.0
12	36.88731313131313	39.0	35.0	39.0	33.0	39.0
13	36.596148148148146	39.0	35.0	39.0	32.0	39.0
14	37.988417508417506	40.0	37.0	41.0	33.0	41.0
15	38.145952861952864	40.0	37.0	41.0	33.0	41.0
16	38.279649831649834	40.0	37.0	41.0	34.0	41.0
17	38.20847138047138	40.0	37.0	41.0	34.0	41.0
18	38.11547474747475	40.0	37.0	41.0	33.0	41.0
19	38.03894949494949	40.0	37.0	41.0	34.0	41.0
20	38.03189225589226	40.0	36.0	41.0	34.0	41.0
21	37.991367003367	40.0	36.0	41.0	34.0	41.0
22	37.974545454545456	40.0	36.0	41.0	34.0	41.0
23	37.94785185185185	40.0	36.0	41.0	34.0	41.0
24	37.811690235690236	40.0	36.0	41.0	33.0	41.0
25	37.718060606060604	40.0	36.0	41.0	33.0	41.0
26	37.5376835016835	39.0	35.0	41.0	33.0	41.0
27	37.50478114478115	39.0	35.0	41.0	33.0	41.0
28	37.44040404040404	39.0	36.0	41.0	33.0	41.0
29	37.445670033670034	39.0	36.0	41.0	33.0	41.0
30	37.3306936026936	39.0	36.0	41.0	33.0	41.0
31	37.18677441077441	39.0	35.0	41.0	32.0	41.0
32	37.0833265993266	39.0	35.0	41.0	32.0	41.0
33	36.92868686868687	39.0	35.0	41.0	31.0	41.0
34	36.71206734006734	39.0	35.0	41.0	31.0	41.0
35	36.615973063973065	39.0	35.0	41.0	31.0	41.0
36	36.38064646464647	39.0	35.0	41.0	30.0	41.0
37	36.33276767676767	39.0	35.0	41.0	30.0	41.0
38	36.23340067340067	39.0	35.0	40.0	30.0	41.0
39	36.124767676767675	39.0	35.0	40.0	30.0	41.0
40	35.97873400673401	39.0	35.0	40.0	29.0	41.0
41	35.84344781144781	39.0	35.0	40.0	28.0	41.0
42	35.75857239057239	39.0	35.0	40.0	27.0	41.0
43	35.703878787878786	39.0	35.0	40.0	27.0	41.0
44	35.57912457912458	38.0	35.0	40.0	27.0	41.0
45	35.50992592592593	38.0	35.0	40.0	27.0	41.0
46	35.340902356902355	38.0	34.0	40.0	26.0	41.0
47	35.259299663299664	38.0	34.0	40.0	25.0	41.0
48	35.13890909090909	38.0	34.0	40.0	24.0	41.0
49	35.010747474747475	38.0	34.0	40.0	24.0	41.0
50	34.896188552188555	38.0	34.0	40.0	24.0	41.0
51	32.89395286195286	36.0	31.0	39.0	18.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	3.0
9	1.0
10	2.0
11	3.0
12	5.0
13	0.0
14	0.0
15	2.0
16	4.0
17	14.0
18	31.0
19	38.0
20	91.0
21	129.0
22	210.0
23	303.0
24	519.0
25	724.0
26	892.0
27	1063.0
28	1140.0
29	1299.0
30	1508.0
31	2124.0
32	2828.0
33	4041.0
34	5440.0
35	6912.0
36	8016.0
37	13648.0
38	14158.0
39	9101.0
40	1.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	42.68956228956229	19.554208754208755	23.33737373737374	14.41885521885522
2	31.468013468013467	27.50976430976431	25.22962962962963	15.792592592592591
3	31.52996632996633	27.105723905723906	26.315151515151513	15.049158249158248
4	28.049831649831653	24.091582491582493	30.177777777777777	17.68080808080808
5	24.04175084175084	36.561616161616165	23.206734006734006	16.18989898989899
6	25.57037037037037	35.90572390572391	24.945454545454545	13.578451178451179
7	45.81414141414141	43.20269360269361	8.290909090909091	2.692255892255892
8	77.51515151515152	8.16969696969697	10.564309764309765	3.7508417508417513
9	69.96902356902356	9.439730639730639	12.363636363636363	8.227609427609428
10	39.76700336700337	28.167003367003367	19.41010101010101	12.655892255892256
11	33.65791245791246	25.003367003367003	25.42491582491582	15.913804713804714
12	31.74949494949495	22.973737373737375	27.39393939393939	17.882828282828285
13	23.13131313131313	26.72996632996633	30.975084175084177	19.163636363636364
14	19.031649831649833	29.6013468013468	29.878787878787875	21.488215488215488
15	17.757575757575758	26.46868686868687	37.31582491582491	18.45791245791246
16	20.334006734006735	26.272053872053874	32.9037037037037	20.49023569023569
17	20.27744107744108	25.663299663299664	30.996632996632993	23.062626262626264
18	21.032996632996635	28.472727272727273	30.777104377104376	19.717171717171716
19	24.215488215488215	27.89360269360269	27.853198653198653	20.037710437710437
20	26.068686868686868	27.34949494949495	29.415488215488217	17.166329966329965
21	23.505723905723904	28.789225589225587	29.22020202020202	18.484848484848484
22	22.0996632996633	25.152861952861954	30.254545454545458	22.492929292929293
23	21.939393939393938	27.718518518518522	30.168350168350166	20.173737373737374
24	21.086868686868687	25.52861952861953	31.637710437710435	21.746801346801348
25	20.102356902356902	29.95690235690236	29.474747474747474	20.465993265993266
26	19.76161616161616	30.626262626262623	28.82154882154882	20.79057239057239
27	20.98181818181818	29.997306397306396	29.797979797979796	19.22289562289562
28	18.647811447811446	27.96902356902357	33.88821548821549	19.494949494949495
29	20.087542087542086	27.699663299663303	30.519865319865318	21.692929292929293
30	20.96969696969697	28.7003367003367	30.54949494949495	19.780471380471383
31	23.705050505050504	26.87946127946128	28.863299663299664	20.552188552188554
32	24.289562289562287	29.880134680134677	27.326599326599325	18.503703703703703
33	22.892929292929292	27.769696969696973	27.816835016835018	21.52053872053872
34	20.973737373737375	27.477441077441078	30.219528619528617	21.32929292929293
35	23.000673400673403	25.504377104377102	28.304377104377103	23.190572390572388
36	22.363636363636363	27.93939393939394	29.309090909090905	20.387878787878787
37	21.671380471380473	29.294276094276096	29.336026936026936	19.698316498316498
38	22.268013468013468	28.676094276094275	27.25925925925926	21.796632996632997
39	22.311111111111114	28.004040404040403	29.393939393939394	20.29090909090909
40	23.45993265993266	26.27878787878788	29.25791245791246	21.003367003367003
41	18.865993265993268	27.75892255892256	29.482828282828283	23.892255892255893
42	19.84781144781145	26.841750841750844	30.004040404040406	23.306397306397304
43	22.541414141414144	26.366329966329964	27.61346801346801	23.47878787878788
44	20.9010101010101	27.9973063973064	28.53872053872054	22.562962962962963
45	19.773737373737372	29.253872053872055	27.354882154882155	23.617508417508418
46	22.556228956228956	30.15218855218855	26.821548821548824	20.47003367003367
47	20.363636363636363	26.412121212121214	31.811447811447813	21.412794612794613
48	21.546127946127946	25.97979797979798	29.443771043771044	23.03030303030303
49	19.84781144781145	25.947474747474747	31.68080808080808	22.523905723905724
50	19.092255892255892	27.66195286195286	29.5003367003367	23.745454545454546
51	20.637037037037036	26.37306397306397	28.119865319865323	24.87003367003367
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	45.0
1	139.0
2	233.0
3	676.5
4	1120.0
5	789.0
6	458.0
7	382.5
8	307.0
9	293.5
10	280.0
11	264.5
12	249.0
13	257.5
14	266.0
15	263.5
16	261.0
17	253.5
18	246.0
19	272.0
20	298.0
21	349.5
22	401.0
23	441.5
24	482.0
25	626.5
26	896.0
27	1021.0
28	1212.0
29	1403.0
30	1691.5
31	1980.0
32	2193.0
33	2406.0
34	2485.0
35	2564.0
36	2553.5
37	2543.0
38	2940.5
39	3338.0
40	3873.5
41	4409.0
42	4723.0
43	5037.0
44	5460.5
45	5884.0
46	6631.5
47	7379.0
48	6324.0
49	5269.0
50	4914.0
51	4559.0
52	4240.0
53	3921.0
54	3601.0
55	3281.0
56	3136.5
57	2992.0
58	3003.5
59	3015.0
60	2862.0
61	2709.0
62	2343.5
63	1978.0
64	1517.5
65	1057.0
66	911.0
67	765.0
68	647.0
69	529.0
70	421.0
71	313.0
72	259.0
73	205.0
74	167.0
75	102.5
76	76.0
77	58.5
78	41.0
79	28.0
80	15.0
81	10.5
82	6.0
83	6.0
84	6.0
85	4.0
86	2.0
87	1.5
88	1.0
89	0.5
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	74250.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.15757575757576
#Duplication Level	Percentage of deduplicated	Percentage of total
1	68.43694940850743	32.95757575757576
2	11.004838213496658	10.599326599326599
3	6.2868808904550155	9.082828282828283
4	4.29286573258383	8.26936026936027
5	3.070727410017619	7.3939393939393945
6	2.2792739883099813	6.585858585858586
7	1.4906172218027238	5.0249158249158254
8	1.020779148138826	3.932659932659933
9	0.6795872136924239	2.9454545454545453
>10	1.3899376345890315	9.154208754208753
>50	0.025169896803423106	0.8148148148148148
>100	0.02237324160304276	3.239057239057239
>500	0.0	0.0
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCG	412	0.5548821548821549	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGC	371	0.49966329966329964	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	358	0.4821548821548821	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTC	314	0.4228956228956229	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCG	280	0.3771043771043771	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGC	244	0.32861952861952864	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	225	0.30303030303030304	No Hit
GAATCTATCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCTTC	201	0.2707070707070707	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCG	97	0.13063973063973064	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGTAGTCCTCGTATGCCGTCT	78	0.10505050505050505	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.05117845117845118	0.0
2	0.0	0.0	0.0	0.27205387205387205	0.0
3	0.0	0.0	0.0	0.33535353535353535	0.0
4	0.0	0.0	0.0	0.4861952861952862	0.0
5	0.0	0.0	0.0	1.2215488215488215	0.0
6	0.0	0.0	0.0	1.4047138047138048	0.0
7	0.0	0.0	0.0	1.7265993265993267	0.0
8	0.0	0.0	0.0	2.1858585858585857	0.0
9	0.0	0.0	0.0	2.334006734006734	0.0
10	0.0	0.0	0.0	3.868013468013468	0.0
11	0.0	0.0	0.0	4.378451178451178	0.0
12	0.0	0.0	0.0	5.806060606060606	0.0
13	0.0	0.0	0.0	6.028282828282828	0.0
14	0.0	0.0	0.0	6.136026936026936	0.0
15	0.0	0.0	0.0	6.367676767676768	0.0
16	0.0	0.0	0.0	6.752861952861953	0.0
17	0.0	0.0	0.0	7.1447811447811445	0.0
18	0.0	0.0	0.0	7.536700336700337	0.0
19	0.0	0.0	0.0	7.777777777777778	0.0
20	0.0	0.0	0.0	8.032323232323233	0.0
21	0.0	0.0	0.0	8.334006734006733	0.0
22	0.0	0.0	0.0	8.703030303030303	0.0
23	0.0	0.0	0.0	9.035690235690236	0.0
24	0.0	0.0	0.0	9.309090909090909	0.0
25	0.0	0.0	0.0	9.55016835016835	0.0
26	0.0	0.0	0.0	9.780471380471381	0.0
27	0.0	0.0	0.0	10.03097643097643	0.0
28	0.0	0.0	0.0	10.272053872053872	0.0
29	0.0	0.0	0.0	10.545454545454545	0.0
30	0.0	0.0	0.0	10.864646464646464	0.0
31	0.0	0.0	0.0	11.147474747474748	0.0
32	0.0	0.0	0.0	11.388552188552188	0.0
33	0.0	0.0	0.0	11.643097643097644	0.0
34	0.0	0.0	0.0	11.93131313131313	0.0
35	0.0	0.0	0.0	12.26936026936027	0.0
36	0.0	0.0	0.0	12.562962962962963	0.0
37	0.0	0.0	0.0	12.847138047138047	0.0
38	0.0	0.0	0.0	13.108417508417508	0.0
39	0.0	0.0	0.0	13.3993265993266	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCCGCCC	35	1.191529E-7	45.000004	35
CTTCCAT	35	1.191529E-7	45.000004	27
TTTCCGC	35	1.191529E-7	45.000004	33
TCCAGAT	35	1.191529E-7	45.000004	18
AGATTAC	35	1.191529E-7	45.000004	21
AAGGACC	45	3.7471182E-10	45.000004	6
ATTCTCC	35	1.191529E-7	45.000004	14
ATTTCCG	35	1.191529E-7	45.000004	32
ATGGGCT	20	6.9906906E-4	45.0	5
GAGTGAC	25	3.855542E-5	45.0	9
CTCGTAC	20	6.9906906E-4	45.0	29
CAAGTGG	20	6.9906906E-4	45.0	27
GTCTCGT	20	6.9906906E-4	45.0	27
TACGGTT	20	6.9906906E-4	45.0	33
AGTGGCA	20	6.9906906E-4	45.0	29
GCGCAGG	20	6.9906906E-4	45.0	3
CGGGGAT	20	6.9906906E-4	45.0	6
AGTGACG	20	6.9906906E-4	45.0	10
TCTCGTA	20	6.9906906E-4	45.0	28
CGAGGGT	20	6.9906906E-4	45.0	5
>>END_MODULE