##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936797.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	193938
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.95186606028731	31.0	31.0	34.0	30.0	34.0
2	32.13174313440378	33.0	31.0	34.0	30.0	34.0
3	31.94584867328734	33.0	31.0	34.0	30.0	34.0
4	35.73696232816673	37.0	35.0	37.0	35.0	37.0
5	31.9953026224876	37.0	35.0	37.0	0.0	37.0
6	33.90467572110675	37.0	35.0	37.0	19.0	37.0
7	20.75710278542627	32.0	0.0	37.0	0.0	37.0
8	28.090632057667914	35.0	17.0	37.0	17.0	37.0
9	34.79211397456919	37.0	32.0	39.0	32.0	39.0
10	36.23905578071342	37.0	35.0	39.0	32.0	39.0
11	36.650166548072065	38.0	35.0	39.0	33.0	39.0
12	37.027668636368325	39.0	37.0	39.0	34.0	39.0
13	36.856077715558584	39.0	37.0	39.0	33.0	39.0
14	38.28436407511679	40.0	38.0	41.0	34.0	41.0
15	38.42221740968763	40.0	38.0	41.0	34.0	41.0
16	38.498370613288785	40.0	38.0	41.0	34.0	41.0
17	38.4304984067073	40.0	38.0	41.0	34.0	41.0
18	38.297904484938485	40.0	37.0	41.0	34.0	41.0
19	38.27773824624364	40.0	37.0	41.0	34.0	41.0
20	38.251688684012414	40.0	37.0	41.0	34.0	41.0
21	38.195681093957866	40.0	37.0	41.0	34.0	41.0
22	38.15994802462642	40.0	37.0	41.0	34.0	41.0
23	38.126622941352394	40.0	37.0	41.0	34.0	41.0
24	38.05383679320195	40.0	37.0	41.0	34.0	41.0
25	37.90401571636296	40.0	36.0	41.0	33.0	41.0
26	37.78096092565665	40.0	36.0	41.0	33.0	41.0
27	37.7599284307356	40.0	36.0	41.0	33.0	41.0
28	37.669064340149944	40.0	36.0	41.0	33.0	41.0
29	37.648145283544224	40.0	36.0	41.0	33.0	41.0
30	37.49825201868639	40.0	36.0	41.0	33.0	41.0
31	37.3691282781095	40.0	36.0	41.0	33.0	41.0
32	37.26757520444678	40.0	36.0	41.0	32.0	41.0
33	37.10216151553589	40.0	36.0	41.0	31.0	41.0
34	36.895028308015966	40.0	36.0	41.0	31.0	41.0
35	36.76706473202776	40.0	35.0	41.0	30.0	41.0
36	36.61708896657695	40.0	35.0	41.0	30.0	41.0
37	36.56782579999794	39.0	35.0	41.0	30.0	41.0
38	36.469232435108125	39.0	35.0	41.0	30.0	41.0
39	36.407104332312386	39.0	35.0	41.0	30.0	41.0
40	36.31864822778414	39.0	35.0	41.0	29.0	41.0
41	36.19552124905898	39.0	35.0	41.0	29.0	41.0
42	36.12437995648094	39.0	35.0	41.0	29.0	41.0
43	36.044230630407654	39.0	35.0	40.0	28.0	41.0
44	35.934056244779256	39.0	35.0	40.0	28.0	41.0
45	35.89032061792944	39.0	35.0	40.0	27.0	41.0
46	35.73984469263373	39.0	35.0	40.0	27.0	41.0
47	35.68911198424239	39.0	35.0	40.0	26.0	41.0
48	35.611417050810054	39.0	35.0	40.0	26.0	41.0
49	35.47858593983644	39.0	35.0	40.0	26.0	41.0
50	35.3506275201353	38.0	34.0	40.0	26.0	41.0
51	33.567758768266145	37.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	4.0
9	10.0
10	8.0
11	8.0
12	6.0
13	1.0
14	4.0
15	6.0
16	15.0
17	28.0
18	52.0
19	122.0
20	212.0
21	374.0
22	612.0
23	890.0
24	1398.0
25	1988.0
26	2338.0
27	2504.0
28	2669.0
29	2973.0
30	3685.0
31	4726.0
32	6231.0
33	8730.0
34	12273.0
35	15797.0
36	21234.0
37	37315.0
38	40664.0
39	27056.0
40	5.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.80769111777991	20.17397312543184	24.8136002227516	13.204735534036653
2	32.958986892718286	25.057492600728065	25.379760542028894	16.603759964524745
3	31.197083604038404	24.911569676907053	28.039889036702455	15.851457682352091
4	27.352040342789962	24.99458589858615	29.33205457414225	18.321319184481638
5	22.514411822334974	38.02658581608555	23.56732564015304	15.891676721426434
6	25.648403097897265	35.52733347770937	26.14804731409007	12.676216110303292
7	48.67019356701626	42.92763666738855	6.051934123276511	2.350235642318679
8	84.27590260804999	4.832472233394178	8.510451793872269	2.3811733646835584
9	77.42732213387784	8.636265198156112	9.644834947251184	4.2915777207148675
10	37.565098124142764	34.973032618671944	16.517649970609163	10.944219286576121
11	28.59986181150677	27.355649743732535	28.581814806793925	15.462673637966773
12	27.47991626189813	24.746568490961028	30.58864173086244	17.184873516278397
13	23.722529880683517	26.04646845899205	31.499757654508144	18.73124400581629
14	20.192020130144687	29.320710742608462	30.486547247058336	20.000721880188514
15	19.197887984819893	29.392898761459847	34.176901896482384	17.23231135723788
16	23.145541358578512	28.07082675906733	31.824088110633298	16.95954377172086
17	22.774804318906043	27.955841557611194	29.428992770885543	19.84036135259722
18	23.282698594396148	28.41732924955398	30.388577792902886	17.911394363146986
19	23.94528148171065	29.74868256865596	28.463220204395217	17.84281574523817
20	25.049758170136847	28.653487196939224	29.43982097371325	16.856933659210675
21	24.64550526456909	27.18703915684394	30.63143891346719	17.53601666511978
22	23.703451618558507	25.73451309181285	30.676814239602347	19.885221050026296
23	21.678577689777146	27.36957171879673	31.416225804122966	19.535624787303156
24	22.00703317555095	26.414111726428036	32.23607544679227	19.342779651228742
25	20.888118883354473	29.526962225040993	30.013200094875682	19.571718796728852
26	20.863884334168652	31.022801101382917	28.660190370118283	19.453124194330147
27	20.65299219338139	30.507688024007674	30.337014922294752	18.502304860316183
28	19.73104806690798	29.81519867174045	32.00043312811311	18.453320133238456
29	21.301137476925618	28.198702678175497	31.091379719291734	19.40878012560715
30	20.885025111117987	28.131155317678846	31.372912992812136	19.61090657839103
31	22.304550938959874	28.88809825820623	29.068568305334697	19.7387824974992
32	22.729944621476967	29.42693025606122	29.89512112118306	17.948004001278758
33	22.437067516422772	29.397023791108502	29.009786632841422	19.156122059627304
34	21.509967102888552	29.189225422557723	30.102919489733832	19.197887984819893
35	21.547092369726407	28.17498375769576	29.54707174457816	20.73085212799967
36	21.697655951902153	30.672689209953695	28.92006723798327	18.709587600160877
37	21.22173065618909	30.917097216636247	29.20881931338882	18.65235281378585
38	21.07168270271943	29.904402437892525	28.36937577988842	20.654539079499635
39	22.562365292000536	28.61481504398313	28.737019047324402	20.08580061669193
40	22.59124049954109	28.13992100568223	30.07816931184193	19.19066918293475
41	20.464787715661707	29.07733399333808	29.678041435922818	20.779836855077395
42	21.309903164928997	28.690612463777082	29.57749383823696	20.421990533056956
43	22.246284895172682	28.90408274809475	28.30028153327352	20.549350823459044
44	21.271231011972898	29.139209438067837	28.411657333787087	21.17790221617218
45	20.918540977013272	29.26141344140911	28.103826996256537	21.71621858532108
46	21.32330951128711	30.14468541492642	28.205921480060635	20.32608359372583
47	21.115511142736338	28.652455939527066	29.96421536779796	20.26781754993864
48	21.43932597015541	27.70524600645567	29.85696459693304	20.998463426455878
49	21.024244861759943	28.198702678175497	29.939465189906052	20.837587270158505
50	19.4572492239788	29.310398168486834	29.517680908331528	21.71467169920284
51	20.5395538780435	28.18632758922955	29.279976074828042	21.994142457898917
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	80.0
1	271.5
2	463.0
3	1371.5
4	2280.0
5	1648.5
6	1017.0
7	898.5
8	780.0
9	767.0
10	754.0
11	758.0
12	762.0
13	744.0
14	726.0
15	700.0
16	674.0
17	727.0
18	780.0
19	807.5
20	835.0
21	952.0
22	1069.0
23	1282.5
24	1496.0
25	1860.5
26	2731.5
27	3238.0
28	3986.5
29	4735.0
30	5228.5
31	5722.0
32	6173.0
33	6624.0
34	7724.0
35	8824.0
36	9298.0
37	9772.0
38	10211.0
39	10650.0
40	11331.0
41	12012.0
42	12760.0
43	13508.0
44	14051.0
45	14594.0
46	14851.5
47	15109.0
48	14592.0
49	14075.0
50	13316.0
51	12557.0
52	11551.0
53	10545.0
54	9716.5
55	8888.0
56	8292.0
57	7696.0
58	7209.0
59	6722.0
60	6083.0
61	5444.0
62	4575.0
63	3706.0
64	3043.0
65	2380.0
66	1829.0
67	1278.0
68	1024.0
69	770.0
70	666.5
71	563.0
72	414.0
73	265.0
74	204.0
75	110.5
76	78.0
77	63.0
78	48.0
79	34.5
80	21.0
81	16.0
82	11.0
83	10.0
84	9.0
85	7.0
86	5.0
87	4.0
88	3.0
89	2.5
90	2.0
91	1.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	193938.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	44.56578906660892
#Duplication Level	Percentage of deduplicated	Percentage of total
1	67.88730764780748	30.25451432932174
2	10.114543561263451	9.015252297125885
3	5.621890547263682	7.516319648547475
4	3.7591114196459565	6.7011106642329
5	2.912183269698022	6.489187266033475
6	2.3140113386555594	6.1875444729759
7	1.807242855489992	5.637884272293207
8	1.4520421150063636	5.1769122090565025
9	1.0447761194029852	4.190514494322928
>10	3.066065023718616	17.53498540770762
>50	0.011570056693277797	0.3588775794326022
>100	0.008099039685294459	0.6094731305881261
>500	0.0011570056693277796	0.32742422836164137
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	635	0.32742422836164137	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	351	0.18098567583454506	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCAGGATATCGTATGCCGTCTTC	195	0.10054759768585836	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.022172034361496975	0.0
2	0.0	0.0	0.0	0.13561034969938845	0.0
3	0.0	0.0	0.0	0.1835638193649517	0.0
4	0.0	0.0	0.0	0.24440800668254803	0.0
5	0.0	0.0	0.0	0.48726912724685206	0.0
6	0.0	0.0	0.0	0.7038331838010086	0.0
7	0.0	0.0	0.0	0.804380781486867	0.0
8	0.0	0.0	0.0	1.0389918427538698	0.0
9	0.0	0.0	0.0	1.1055079458383608	0.0
10	0.0	0.0	0.0	1.4638698965648815	0.0
11	0.0	0.0	0.0	1.7412781404366344	0.0
12	0.0	0.0	0.0	2.112015180109107	0.0
13	0.0	0.0	0.0	2.2393754705111943	0.0
14	0.0	0.0	0.0	2.315172890305149	0.0
15	0.0	0.0	0.0	2.408501686105869	0.0
16	0.0	0.0	0.0	2.577112272994462	0.0
17	0.0	0.0	0.0	2.8091451907310585	0.0
18	0.0	0.0	0.0	3.057678227062257	0.0
19	0.0	0.0	0.0	3.220616898183956	0.0
20	0.0	0.0	0.0	3.3897431137786302	0.0
21	0.0	0.0	0.0	3.5836195072652086	0.0
22	0.0	0.0	0.0	3.80998050923491	0.0
23	0.0	0.0	0.0	4.065216718745166	0.0
24	0.0	0.0	0.0	4.282812032711485	0.0
25	0.0	0.0	0.0	4.446266332539265	0.0
26	0.0	0.0	0.0	4.598376800833256	0.0
27	0.0	0.0	0.0	4.7711124173704995	0.0
28	0.0	0.0	0.0	4.944363662613825	0.0
29	0.0	0.0	0.0	5.145458857985542	0.0
30	0.0	0.0	0.0	5.372335488661324	0.0
31	0.0	0.0	0.0	5.599212119337108	0.0
32	0.0	0.0	0.0	5.784322824820303	0.0
33	0.0	0.0	0.0	6.007074425847436	0.0
34	0.0	0.0	0.0	6.2071383638069895	0.0
35	0.0	0.0	0.0	6.454124514019944	0.0
36	5.15628706081325E-4	0.0	0.0	6.686673060462622	0.0
37	5.15628706081325E-4	0.0	0.0	6.93881549773639	0.0
38	5.15628706081325E-4	0.0	0.0	7.197661108189215	0.0
39	5.15628706081325E-4	0.0	0.0	7.462694263115016	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GCACCGG	20	7.01877E-4	45.000004	20
TCTCGGT	20	7.01877E-4	45.000004	45
GTCAACC	20	7.01877E-4	45.000004	16
GGGTTCG	20	7.01877E-4	45.000004	8
AATACGG	20	7.01877E-4	45.000004	2
AGGTATC	25	3.8787788E-5	45.0	7
ACACGGG	25	3.8787788E-5	45.0	3
CACGGGA	35	1.2049532E-7	45.0	4
CGACGGG	45	3.8198777E-10	45.0	3
CGTTGAG	25	3.8787788E-5	45.0	2
ATTACGG	25	3.8787788E-5	45.0	2
GCACGAA	30	2.1561427E-6	44.999996	1
CGTTTTA	185	0.0	43.783783	1
CGTTATT	100	0.0	42.75	1
AGGCGAT	50	1.0713848E-9	40.5	7
ACGGGAG	90	0.0	40.0	5
TGCGGGA	85	0.0	39.705883	4
GGCGATT	40	3.4384357E-7	39.375004	8
ATACGGG	40	3.4384357E-7	39.375004	3
GTGCGGG	75	0.0	39.0	3
>>END_MODULE