##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936794.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	102761
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.830529091776064	31.0	31.0	34.0	30.0	34.0
2	31.99792722920174	33.0	31.0	34.0	30.0	34.0
3	31.80185089674098	33.0	31.0	34.0	30.0	34.0
4	35.64147877112912	37.0	35.0	37.0	35.0	37.0
5	31.910218857348607	37.0	35.0	37.0	0.0	37.0
6	33.80430318895301	37.0	35.0	37.0	19.0	37.0
7	20.861494146612042	32.0	0.0	37.0	0.0	37.0
8	28.028454374714144	35.0	17.0	37.0	17.0	37.0
9	34.63005420344294	35.0	32.0	39.0	28.0	39.0
10	36.09467599575714	37.0	35.0	39.0	32.0	39.0
11	36.51263611681475	37.0	35.0	39.0	32.0	39.0
12	36.79327760531719	39.0	35.0	39.0	33.0	39.0
13	36.41649069199404	39.0	35.0	39.0	32.0	39.0
14	37.82897208084779	40.0	37.0	41.0	33.0	41.0
15	38.00902093206567	40.0	37.0	41.0	33.0	41.0
16	38.163126088691236	40.0	37.0	41.0	34.0	41.0
17	38.088399295452554	40.0	36.0	41.0	33.0	41.0
18	37.947976372359165	40.0	36.0	41.0	33.0	41.0
19	37.89934897480562	40.0	36.0	41.0	34.0	41.0
20	37.9015385214235	40.0	36.0	41.0	34.0	41.0
21	37.833711232860715	39.0	36.0	41.0	33.0	41.0
22	37.80246396979399	39.0	36.0	41.0	33.0	41.0
23	37.826403012816144	39.0	36.0	41.0	34.0	41.0
24	37.685590836990684	39.0	35.0	41.0	33.0	41.0
25	37.572843783147306	39.0	35.0	41.0	33.0	41.0
26	37.42942361401699	39.0	35.0	41.0	33.0	41.0
27	37.431175251311295	39.0	35.0	41.0	33.0	41.0
28	37.39984040637985	39.0	35.0	41.0	33.0	41.0
29	37.367435116435225	39.0	35.0	41.0	33.0	41.0
30	37.2647989023073	39.0	35.0	41.0	33.0	41.0
31	37.09713801928747	39.0	35.0	41.0	32.0	41.0
32	36.770136530395774	39.0	35.0	41.0	31.0	41.0
33	36.61390994638044	39.0	35.0	41.0	31.0	41.0
34	36.333988575432315	39.0	35.0	41.0	30.0	41.0
35	36.16112143712109	39.0	35.0	41.0	30.0	41.0
36	35.998919823668516	39.0	35.0	40.0	29.0	41.0
37	35.81877365926762	39.0	35.0	40.0	27.0	41.0
38	35.72470100524518	39.0	35.0	40.0	27.0	41.0
39	35.34840065783712	39.0	35.0	40.0	24.0	41.0
40	35.135099891982364	38.0	34.0	40.0	24.0	41.0
41	34.94190402973891	38.0	34.0	40.0	23.0	41.0
42	34.958165062621035	38.0	34.0	40.0	23.0	41.0
43	34.75553955294324	38.0	34.0	40.0	23.0	41.0
44	34.54291024805131	38.0	34.0	40.0	21.0	41.0
45	34.551892254843764	38.0	34.0	40.0	22.0	41.0
46	34.21220112688666	38.0	33.0	40.0	19.0	41.0
47	34.07090238514611	38.0	33.0	40.0	18.0	41.0
48	33.91656367688131	38.0	33.0	40.0	17.0	41.0
49	33.80529578341978	38.0	33.0	40.0	15.0	41.0
50	33.77471024999757	38.0	33.0	40.0	17.0	41.0
51	31.665495664697698	35.0	29.0	39.0	10.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
9	1.0
10	4.0
11	3.0
12	2.0
13	2.0
14	8.0
15	6.0
16	6.0
17	18.0
18	27.0
19	63.0
20	128.0
21	205.0
22	356.0
23	604.0
24	1010.0
25	1479.0
26	1782.0
27	1916.0
28	2114.0
29	2261.0
30	2603.0
31	3077.0
32	3966.0
33	5402.0
34	7750.0
35	9356.0
36	10476.0
37	17491.0
38	18641.0
39	12004.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.7990969336616	17.223460262161716	22.685649224900498	15.291793579276183
2	32.07637138603167	29.57639571432742	23.291910355095805	15.05532254454511
3	29.27180545148451	27.823785288192994	27.60385749457479	15.300551765747706
4	27.06863498798182	22.921147127801405	32.27489027938615	17.735327604830626
5	23.081713879779294	34.80114051050496	24.298128667490584	17.81901694222516
6	23.116746625665378	34.79238232403344	28.71225464913732	13.378616401163864
7	45.37616410895184	42.501532682632515	9.221397222681757	2.9009059857338872
8	76.80832222341161	7.4785181148490185	11.732077344517862	3.981082317221514
9	71.20600227712848	7.421103336869046	12.046398925662459	9.326495460340013
10	40.31587859207287	24.842109360555074	20.89411352555931	13.947898521812748
11	31.466217728515684	25.439612304278857	27.418962446842677	15.675207520362783
12	31.815572055546365	23.065170638666423	29.19395490507099	15.925302400716227
13	22.528001868413114	27.700197545761522	30.806434347661078	18.965366238164282
14	15.843559326982026	33.99636048695517	29.491733245102715	20.66834694096009
15	15.207131110051478	28.14978445129962	40.652582205311354	15.99050223333755
16	17.975691166882378	27.016085869152697	35.29257208474032	19.715650879224608
17	17.276009380990843	26.866223567306662	29.572503187006742	26.28526386469575
18	18.848590418544	29.559852473214548	32.11140413191775	19.4801529763237
19	21.59379531145084	29.90628740475472	27.056957406019794	21.44295987777464
20	23.361975846867974	27.29440157258104	32.31770807991359	17.025914500637402
21	21.465341909868528	30.10091377078853	30.708148032814	17.725596286528937
22	20.039703778670898	25.00754177168381	29.548174891252515	25.404579558392776
23	18.942011074240227	28.474810482576075	31.52655190198616	21.056626541197538
24	20.411440137795466	24.56573992078707	33.1750372222925	21.847782719124957
25	17.928980839034264	31.009818900166408	30.777240392756006	20.283959868043326
26	17.488152119967694	30.434697988536506	29.67565516100466	22.40149473049114
27	19.687430056149708	32.01019842158017	28.00478780860443	20.29758371366569
28	15.398838080594778	28.638296630044476	34.58121271688675	21.381652572473993
29	17.697375463454033	25.822053113535294	32.33327818919629	24.147293233814384
30	17.51831920670293	27.62040073568766	31.549907065910222	23.311372991699187
31	21.97915551619778	27.154270589036695	27.667111063535778	23.19946283122975
32	22.405387257811814	26.36408754293944	31.373770204649627	19.856754994599118
33	20.83085995659832	28.023277313377644	28.72101283560884	22.424849894415196
34	20.667373809129924	26.652134564669478	29.392473798425474	23.28801782777513
35	19.304989246893275	24.296182403830247	32.830548554412665	23.568279794863813
36	21.983048043518455	28.172166483393507	28.232500656863984	21.612284816224054
37	18.814530804488083	29.112211831336793	32.173684569048575	19.899572795126556
38	17.40057025525248	28.65581300298751	30.17292552622104	23.77069121553897
39	22.34213368885083	26.87887428109886	30.07074668405329	20.70824534599702
40	22.02683897587606	24.868383919969638	33.48741253977676	19.61736456437754
41	18.484639114060784	29.351602261558373	29.177411663958114	22.98634696042273
42	19.608606377906014	25.38025126263855	31.551853329570555	23.45928902988488
43	20.03386498768988	28.095289068810153	27.910394021078034	23.960451922421928
44	19.841184885316412	27.732310896157102	29.700956588589055	22.725547629937427
45	18.876811241618903	29.34479033874719	27.60191123091445	24.176487188719456
46	21.329103453644866	30.27802376387929	27.700197545761522	20.692675236714315
47	20.232383881044367	25.997216842965713	32.079290781522175	21.691108494467745
48	21.239575325269314	24.739930518387325	31.301758449217115	22.718735707126246
49	18.62574322943529	25.388036317279898	32.024795399032705	23.9614250542521
50	17.636068158153385	26.640456982707448	32.49287180934401	23.230603049795157
51	18.79701443154504	26.60055857767052	29.78853845330427	24.81388853748017
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	58.0
1	176.0
2	294.0
3	940.0
4	1586.0
5	1131.0
6	676.0
7	620.0
8	564.0
9	535.5
10	507.0
11	481.5
12	456.0
13	431.5
14	407.0
15	377.5
16	348.0
17	357.5
18	367.0
19	380.0
20	393.0
21	393.0
22	393.0
23	448.5
24	504.0
25	714.5
26	1098.0
27	1271.0
28	1596.0
29	1921.0
30	2268.5
31	2616.0
32	2959.5
33	3303.0
34	3508.5
35	3714.0
36	3851.0
37	3988.0
38	4362.5
39	4737.0
40	5689.0
41	6641.0
42	7459.0
43	8277.0
44	8916.5
45	9556.0
46	10303.5
47	11051.0
48	9647.0
49	8243.0
50	7636.0
51	7029.0
52	6162.0
53	5295.0
54	4544.0
55	3793.0
56	3587.0
57	3381.0
58	3219.0
59	3057.0
60	2830.5
61	2604.0
62	2349.5
63	2095.0
64	1615.0
65	1135.0
66	810.0
67	485.0
68	435.5
69	386.0
70	336.0
71	286.0
72	240.0
73	194.0
74	151.0
75	73.0
76	38.0
77	29.0
78	20.0
79	21.0
80	22.0
81	20.0
82	18.0
83	13.0
84	8.0
85	5.0
86	2.0
87	3.0
88	4.0
89	3.0
90	2.0
91	2.5
92	3.0
93	1.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	102761.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	38.90775683381828
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.76979640838377	27.924017866700403
2	7.513381021459657	5.84657603565555
3	3.9517783002351057	4.612644875001217
4	2.8362763243459557	4.414125981646733
5	2.0734330448701916	4.033631436050642
6	1.853334000300135	4.326544116931521
7	1.470661797809014	4.00541061297574
8	1.4281426641988895	4.445266200212142
9	1.2055424941223551	4.221445879273265
>10	5.820119053574109	31.605375580229854
>50	0.047521384623080386	1.3458413211237725
>100	0.027512380571257065	2.640106655248587
>500	0.002501125506477915	0.5790134389505746
>1k	0.0	0.0
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCG	595	0.5790134389505746	No Hit
GAATCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC	492	0.4787808604431642	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	474	0.4612644875001216	No Hit
GAATGAAACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCG	296	0.28804702173003377	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC	273	0.265664989636146	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	272	0.264691857805977	No Hit
GAATCTATCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC	241	0.23452477107073696	No Hit
GAATGAAACGGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGC	176	0.1712712021097498	No Hit
GAATATGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTTC	130	0.1265071379219743	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCTT	124	0.12066834694096008	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCG	121	0.117748951450453	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCCCTTGTATCGTATGCCGTCT	114	0.11093702863926977	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.04476406418777552	0.0
2	0.0	0.0	0.0	0.3950915230486274	0.0
3	0.0	0.0	0.0	0.471968937631981	0.0
4	0.0	0.0	0.0	0.7269294771362677	0.0
5	0.0	0.0	0.0	2.036764920543786	0.0
6	0.0	0.0	0.0	2.627455941456389	0.0
7	0.0	0.0	0.0	3.094559219937525	0.0
8	0.0	0.0	0.0	3.7864559511877074	0.0
9	0.0	0.0	0.0	3.9197750119208647	0.0
10	0.0	0.0	0.0	5.945835482332791	0.0
11	0.0	0.0	0.0	6.505386284679986	0.0
12	0.0	0.0	0.0	7.784081509522095	0.0
13	0.0	0.0	0.0	8.145113418514807	0.0
14	0.0	0.0	0.0	8.313465225134049	0.0
15	0.0	0.0	0.0	8.551882523525462	0.0
16	0.0	0.0	0.0	8.877881686632088	0.0
17	0.0	0.0	0.0	9.190257004116347	0.0
18	0.0	0.0	0.0	9.647628964295793	0.0
19	0.0	0.0	0.0	9.85198664863129	0.0
20	0.0	0.0	0.0	10.07386070590983	0.0
21	0.0	0.0	0.0	10.344391354696821	0.0
22	0.0	0.0	0.0	10.693745681727505	0.0
23	0.0	0.0	0.0	11.008067262872101	0.0
24	0.0	0.0	0.0	11.266920329697063	0.0
25	0.0	0.0	0.0	11.520907737371182	0.0
26	0.0	0.0	0.0	11.76419069491344	0.0
27	0.0	0.0	0.0	11.991903543172993	0.0
28	0.0	0.0	0.0	12.18555677737663	0.0
29	0.0	0.0	0.0	12.410350230145678	0.0
30	0.0	0.0	0.0	12.674068956121486	0.0
31	0.0	0.0	0.0	12.95141152771966	0.0
32	0.0	0.0	0.0	13.19274822160158	0.0
33	0.0	0.0	0.0	13.401971565087923	0.0
34	0.0	0.0	0.0	13.620926226875955	0.0
35	0.0	0.0	0.0	13.878806161870749	0.0
36	0.0	0.0	0.0	14.121115987582838	0.0
37	0.0	0.0	0.0	14.326446803748503	0.0
38	0.0	0.0	0.0	14.586273002403635	0.0
39	0.0	0.0	0.0	14.796469477720146	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GGGTACT	30	2.1461892E-6	45.000004	7
GAAAGGC	35	1.1975499E-7	45.000004	4
CTAGTCT	30	2.1461892E-6	45.000004	28
TCCAATG	35	1.1975499E-7	45.000004	16
TAAGGGT	30	2.1461892E-6	45.000004	5
GGCGATG	30	2.1461892E-6	45.000004	8
CACTTAC	30	2.1461892E-6	45.000004	37
TTTAGGC	20	7.003304E-4	45.0	37
GGGTGTA	25	3.8659742E-5	45.0	8
GGTACTT	20	7.003304E-4	45.0	8
GCAAGGT	20	7.003304E-4	45.0	4
GCGACTG	20	7.003304E-4	45.0	11
CTCGTAC	25	3.8659742E-5	45.0	29
ATAGGTA	25	3.8659742E-5	45.0	5
CGACGTT	20	7.003304E-4	45.0	27
ACGTTTT	20	7.003304E-4	45.0	29
GAAGACG	25	3.8659742E-5	45.0	45
TTTCGAC	20	7.003304E-4	45.0	24
TATGGGC	25	3.8659742E-5	45.0	4
TGGGTAC	25	3.8659742E-5	45.0	6
>>END_MODULE