FastQCFastQC Report
Sat 14 Jan 2017
SRR2936791.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936791.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences266690
Sequences flagged as poor quality0
Sequence length51
%GC46

[FAIL]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[WARN]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[WARN]Overrepresented sequences

SequenceCountPercentagePossible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT13860.5197045258539877No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT8790.3295961603359706No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3170.11886459934755708No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT2790.10461584611346508No Hit

[WARN]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
CGTTATT1150.045.0000041
TGACCGA302.1592114E-645.00000416
AGACGCG207.023534E-445.010
GGGCGAA207.023534E-445.01
TTGCGAA253.882723E-545.01
CGGTCTA253.882723E-545.031
AGCACGG207.023534E-445.02
CGTTTTA3150.042.1428571
TACGAAT750.042.00000412
ATCCAAG1150.041.0869644
GCATATC1100.040.90909228
TGCGAAG501.0750227E-940.52
CGAGATA451.918852E-840.019
ATATCAC1250.039.60000230
CGTTTTT5250.039.4285741
CATATCA1200.039.37500429
TGCATAT1200.039.37500427
CTACGAA800.039.37511
CGAATAT800.039.37514
TTACACG403.4449113E-739.37534
Produced by FastQC (version 0.11.5)