##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936791.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	266690
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	fail
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.998267651580488	33.0	31.0	34.0	30.0	34.0
2	32.187536090592076	33.0	31.0	34.0	30.0	34.0
3	31.973302336045595	33.0	31.0	34.0	30.0	34.0
4	35.77728823727924	37.0	35.0	37.0	35.0	37.0
5	32.04572349919382	37.0	35.0	37.0	0.0	37.0
6	33.94518729611159	37.0	35.0	37.0	19.0	37.0
7	20.70856425062807	32.0	0.0	37.0	0.0	37.0
8	28.054006524429113	35.0	17.0	37.0	17.0	37.0
9	34.81939330308598	37.0	32.0	39.0	32.0	39.0
10	36.28841726348944	37.0	35.0	39.0	32.0	39.0
11	36.719929506168214	38.0	37.0	39.0	33.0	39.0
12	37.06089467171623	39.0	37.0	39.0	34.0	39.0
13	36.83758296149087	39.0	37.0	39.0	33.0	39.0
14	38.181806591923205	40.0	38.0	41.0	33.0	41.0
15	38.38271776219581	40.0	38.0	41.0	34.0	41.0
16	38.46691664479358	40.0	38.0	41.0	34.0	41.0
17	38.43950654317747	40.0	38.0	41.0	34.0	41.0
18	38.3341782594023	40.0	38.0	41.0	34.0	41.0
19	38.2669353931531	40.0	37.0	41.0	34.0	41.0
20	38.24525853987776	40.0	37.0	41.0	34.0	41.0
21	38.157647455847616	40.0	37.0	41.0	34.0	41.0
22	38.118043421200646	40.0	37.0	41.0	34.0	41.0
23	38.07915932355919	40.0	37.0	41.0	34.0	41.0
24	37.93799917507218	40.0	36.0	41.0	33.0	41.0
25	37.82460909670404	40.0	36.0	41.0	33.0	41.0
26	37.676418313397576	40.0	36.0	41.0	33.0	41.0
27	37.622614271251265	40.0	36.0	41.0	33.0	41.0
28	37.54132513405077	40.0	36.0	41.0	33.0	41.0
29	37.523300461209644	40.0	36.0	41.0	33.0	41.0
30	37.36114589973377	40.0	36.0	41.0	33.0	41.0
31	37.259548539502795	40.0	35.0	41.0	32.0	41.0
32	37.14249128201283	40.0	35.0	41.0	32.0	41.0
33	36.967902808504256	39.0	35.0	41.0	31.0	41.0
34	36.778458134913194	39.0	35.0	41.0	31.0	41.0
35	36.611713975027186	39.0	35.0	41.0	30.0	41.0
36	36.43642806254453	39.0	35.0	41.0	30.0	41.0
37	36.36854775207169	39.0	35.0	41.0	30.0	41.0
38	36.23101728598748	39.0	35.0	41.0	29.0	41.0
39	36.13957778694364	39.0	35.0	41.0	29.0	41.0
40	35.99202069818891	39.0	35.0	41.0	28.0	41.0
41	35.82856500056245	39.0	35.0	40.0	27.0	41.0
42	35.7517717199745	39.0	35.0	40.0	27.0	41.0
43	35.69941880085493	39.0	35.0	40.0	27.0	41.0
44	35.54079643031235	39.0	35.0	40.0	26.0	41.0
45	35.46748284525104	38.0	35.0	40.0	26.0	41.0
46	35.32503655930106	38.0	34.0	40.0	26.0	41.0
47	35.26627545089805	38.0	34.0	40.0	25.0	41.0
48	35.13315834864449	38.0	34.0	40.0	24.0	41.0
49	35.02635269413926	38.0	34.0	40.0	24.0	41.0
50	34.89242566275451	38.0	34.0	40.0	24.0	41.0
51	33.064914319997	36.0	31.0	39.0	19.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	5.0
8	7.0
9	18.0
10	22.0
11	9.0
12	8.0
13	5.0
14	5.0
15	15.0
16	25.0
17	61.0
18	94.0
19	177.0
20	282.0
21	497.0
22	788.0
23	1296.0
24	1910.0
25	2761.0
26	3358.0
27	3783.0
28	4047.0
29	4491.0
30	5544.0
31	6613.0
32	8949.0
33	13188.0
34	17912.0
35	22908.0
36	29877.0
37	50225.0
38	52920.0
39	34887.0
40	3.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.407214368742736	20.398215156173833	24.62934493231842	14.56522554276501
2	32.80587948554501	24.403614683715176	26.018598372642394	16.771907458097417
3	32.03982151561738	23.410326596422813	29.104578349394426	15.445273538565377
4	27.781319134575728	25.826240203982152	28.44613596310323	17.946304698338896
5	22.351419250815553	37.27698826352694	24.243128726236453	16.12846375942105
6	25.640631444748585	34.34249503168473	26.52030447335858	13.496569050208107
7	47.750196857774945	43.39945254790206	6.176084592598148	2.6742660017248494
8	82.9821140650193	5.080805429524917	9.123701676103341	2.8133788293524318
9	76.61704600847426	8.008549251940455	10.506580674191008	4.867824065394278
10	40.99628782481533	28.15816116089842	17.939930256102592	12.905620758183659
11	30.713562563275715	25.982976489557164	25.845363530691063	17.458097416476058
12	30.015748621995574	23.038734110765308	29.005212043946155	17.940305223292963
13	25.30128613746297	24.006524429112453	30.099741272638642	20.59244816078593
14	19.681652855375155	26.269826390190858	30.88079793018111	23.167722824252877
15	19.934380741685104	26.31407251865462	33.886159961003415	19.86538677865687
16	24.225505268289023	25.172297423975404	31.85271288762233	18.74948442011324
17	23.1699726273951	25.871611234017024	29.651280512955115	21.307135625632757
18	23.821290637069257	26.93014361243391	29.420675690877047	19.827890059619783
19	24.817578461884583	27.194120514454983	28.395890359593533	19.592410664066897
20	26.94776707038134	26.215456147587084	28.774607221868088	18.062169560163486
21	26.409314185008814	26.206456935018185	29.129701151149273	18.254527728823728
22	25.02006074468484	23.612433912032696	30.206981889084705	21.160523454197758
23	24.368742735010688	25.204169635156926	30.770182609021713	19.656905020810676
24	23.069856387566087	25.022685515017436	31.461247140875177	20.446210956541304
25	23.343582436536803	26.54955191420751	29.148824477858188	20.958041171397504
26	21.560238479133076	28.307398102666014	28.671116277325737	21.461247140875173
27	21.744347369605162	28.26727661329634	29.601784843826167	20.38659117327234
28	20.59169822640519	28.147662079568036	30.44471108777982	20.815928606246953
29	21.783718924594098	26.69691402002325	30.28085042558776	21.238516629794894
30	22.677265739247815	26.503805916982266	29.993625557763696	20.825302786006226
31	23.976152086692416	26.79628032547152	28.401514867449094	20.82605272038697
32	25.071431249765645	27.33360830927294	27.612583898908845	19.98237654205257
33	24.328246278450635	26.55817615958604	28.347519592035695	20.76605796992763
34	22.505530766057973	26.639544039896506	29.939255315159922	20.9156698788856
35	23.377329483670177	26.739285312535156	28.632869623907908	21.250515579886763
36	23.063106978139412	28.897971427500096	27.21361880835427	20.825302786006226
37	22.90449585661255	28.380516704788334	28.203157223742924	20.5118302148562
38	22.185683752671643	28.395140425212794	28.34077018260902	21.078405639506542
39	23.02711012786381	27.197870186358692	28.645618508380515	21.12940117739698
40	24.037646705913232	26.171584986313697	29.511792718137166	20.278975589635905
41	20.518954591473246	26.777156998762607	30.180734185758745	22.5231542240054
42	20.98728861224643	27.309985376279577	29.158573624807833	22.544152386666166
43	21.93145599760021	27.496344069893887	28.626120214481233	21.946079718024674
44	21.767595335408153	27.24961565862987	28.97071506243204	22.01207394352994
45	21.812966365443025	26.90877048258277	28.249278188158538	23.028984963815667
46	21.48636994263002	28.71723724174135	27.58371142525029	22.212681390378343
47	21.76722036821778	26.837526716412313	29.875510892796882	21.519742022573023
48	21.817465971727472	26.682665266789158	30.099741272638642	21.400127488844724
49	21.418500881172896	27.293861787093633	29.54179009336683	21.745847238366643
50	20.589073456072594	27.390978289399676	29.989875885859984	22.030072368667742
51	20.98841351381754	26.952266676665793	29.221943079980502	22.837376729536167
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	152.0
1	424.5
2	697.0
3	2525.0
4	4353.0
5	2955.5
6	1558.0
7	1342.0
8	1126.0
9	1145.0
10	1164.0
11	1093.5
12	1023.0
13	985.0
14	947.0
15	942.0
16	937.0
17	901.5
18	866.0
19	966.0
20	1066.0
21	1213.0
22	1360.0
23	1588.0
24	1816.0
25	2161.5
26	3060.5
27	3614.0
28	4364.0
29	5114.0
30	5791.5
31	6469.0
32	7286.0
33	8103.0
34	8783.0
35	9463.0
36	9921.5
37	10380.0
38	11369.0
39	12358.0
40	13386.5
41	14415.0
42	15058.0
43	15701.0
44	16144.0
45	16587.0
46	17112.0
47	17637.0
48	17125.5
49	16614.0
50	16134.0
51	15654.0
52	15193.5
53	14733.0
54	14320.5
55	13908.0
56	14061.0
57	14214.0
58	14012.5
59	13811.0
60	12849.5
61	11888.0
62	10558.0
63	9228.0
64	7744.5
65	6261.0
66	5192.0
67	4123.0
68	3374.0
69	2625.0
70	2201.0
71	1777.0
72	1453.0
73	1129.0
74	903.5
75	489.5
76	301.0
77	217.0
78	133.0
79	114.0
80	95.0
81	78.5
82	62.0
83	41.0
84	20.0
85	16.0
86	12.0
87	8.5
88	5.0
89	4.0
90	3.0
91	2.0
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	266690.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	39.827546149085705
#Duplication Level	Percentage of deduplicated	Percentage of total
1	60.69877823258034	24.174833912512124
2	11.808143888847905	9.405787913362687
3	7.02954290362926	8.399083332038169
4	5.133821266432566	8.178700136400026
5	3.7922046901055326	7.551710365097867
6	2.939966431011905	7.025498922473367
7	2.241543949517159	6.2492636566218955
8	1.7145264409016023	5.462830475906899
9	1.3110491261298296	4.699428261718885
>10	3.298543460427663	16.754705826366706
>50	0.020920994335677966	0.5626298990274126
>100	0.008966140429576271	0.6368272129330023
>500	9.962378255084746E-4	0.3487670530642424
>1k	9.962378255084746E-4	0.5499330324767234
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1386	0.5197045258539877	No Hit
CGTTTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	879	0.3295961603359706	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	317	0.11886459934755708	No Hit
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	279	0.10461584611346508	No Hit
>>END_MODULE
>>Adapter Content	warn
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.017248490757058758	0.0
2	0.0	0.0	0.0	0.08061794592973115	0.0
3	0.0	0.0	0.0	0.11623982901496119	0.0
4	0.0	0.0	0.0	0.16723536690539578	0.0
5	0.0	0.0	0.0	0.3569687652330421	0.0
6	0.0	0.0	0.0	0.5092054445236042	0.0
7	0.0	0.0	0.0	0.6123214218755859	0.0
8	0.0	0.0	0.0	0.8433012111440249	0.0
9	0.0	0.0	0.0	0.9554164010649068	0.0
10	0.0	0.0	0.0	1.2775132175934605	0.0
11	0.0	0.0	0.0	1.5257414976189583	0.0
12	0.0	0.0	0.0	1.8065919232067194	0.0
13	0.0	0.0	0.0	1.9048333270838802	0.0
14	0.0	0.0	0.0	1.9644531103528442	0.0
15	0.0	0.0	0.0	2.0495706625670254	0.0
16	0.0	0.0	0.0	2.181934080767933	0.0
17	0.0	0.0	0.0	2.3352956616296074	0.0
18	0.0	0.0	0.0	2.5111552739135328	0.0
19	0.0	0.0	0.0	2.628894971689977	0.0
20	0.0	0.0	0.0	2.746259702276051	0.0
21	0.0	0.0	0.0	2.910120364468109	0.0
22	0.0	0.0	0.0	3.076605796992763	0.0
23	0.0	0.0	0.0	3.234466984138888	0.0
24	0.0	0.0	0.0	3.355206419438299	0.0
25	0.0	0.0	0.0	3.4778206906895646	0.0
26	0.0	0.0	0.0	3.596310322846751	0.0
27	0.0	0.0	0.0	3.734673216093592	0.0
28	0.0	0.0	0.0	3.8734110765308034	0.0
29	0.0	0.0	0.0	4.029022460534703	0.0
30	0.0	0.0	0.0	4.195507893059357	0.0
31	0.0	0.0	0.0	4.354868948966965	0.0
32	0.0	0.0	0.0	4.4981064156886275	0.0
33	0.0	0.0	0.0	4.647718324646593	0.0
34	0.0	0.0	0.0	4.81082905245791	0.0
35	0.0	0.0	0.0	5.005062057070006	0.0
36	0.0	0.0	0.0	5.172297423975402	0.0
37	0.0	0.0	0.0	5.342157561213394	0.0
38	0.0	0.0	0.0	5.552514155011436	0.0
39	0.0	0.0	0.0	5.818740860174735	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGTTATT	115	0.0	45.000004	1
TGACCGA	30	2.1592114E-6	45.000004	16
AGACGCG	20	7.023534E-4	45.0	10
GGGCGAA	20	7.023534E-4	45.0	1
TTGCGAA	25	3.882723E-5	45.0	1
CGGTCTA	25	3.882723E-5	45.0	31
AGCACGG	20	7.023534E-4	45.0	2
CGTTTTA	315	0.0	42.142857	1
TACGAAT	75	0.0	42.000004	12
ATCCAAG	115	0.0	41.08696	44
GCATATC	110	0.0	40.909092	28
TGCGAAG	50	1.0750227E-9	40.5	2
CGAGATA	45	1.918852E-8	40.0	19
ATATCAC	125	0.0	39.600002	30
CGTTTTT	525	0.0	39.428574	1
CATATCA	120	0.0	39.375004	29
TGCATAT	120	0.0	39.375004	27
CTACGAA	80	0.0	39.375	11
CGAATAT	80	0.0	39.375	14
TTACACG	40	3.4449113E-7	39.375	34
>>END_MODULE