FastQCFastQC Report
Sat 14 Jan 2017
SRR2936788.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936788.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences405860
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTC378389.322919233233135No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCG273136.729660474055092No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGC261646.446557926378555No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT106992.6361306854580397No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA95702.3579559453998913No Hit
GAACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCT70291.7318779874833687No Hit
GAATGACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCT65601.6163208988321096No Hit
GCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC63291.5594047208396984No Hit
GAATATGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTC60001.4783422855171735No Hit
CGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG57771.4233972305721185No Hit
GAATGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTT53741.3241019070615483No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTC52741.2994628689695955No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCG44481.0959444143300645No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGC38440.947124624254669No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTT32690.80545015522594No Hit
CGTCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCT29010.7147784950475533No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTA27430.6758488148622678No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTT24050.592568866111467No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCT19950.4915488099344602No Hit
CGTTCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTC19160.4720839698418174No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT17690.4358645838466466No Hit
CCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC16660.41048637461193516No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA16380.40358744394618834No Hit
AAAACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTC16370.40334105356526884No Hit
CTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGCT15010.3698319617602129No Hit
TGAATCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTT14750.3634258118563051No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCC11880.2927117725324003No Hit
GCTGACTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTGC11510.28359532843837776No Hit
GAACAGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCT10860.2675799536786084No Hit
GAATAACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCT10680.26314492682205687No Hit
GCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTTC10310.2540284827280343No Hit
AAAAACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTT10090.24860789434780467No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT9730.23973784063470163No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTC9640.23752032720642585No Hit
CGCTATCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTG9420.2320997388261962No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATG9010.22199773320849556No Hit
GAATGATACGGCGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTAT8960.2207657813038979No Hit
CGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCTT8830.217562706351944No Hit
GAATACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTT8310.20475040654412852No Hit
GAATAATCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTC7940.19563396245010595No Hit
GAATGATACGGCGACCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGT7190.17715468388114128No Hit
GAAAACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTT7130.1756763415956241No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCC6880.1695165820726359No Hit
TGAACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTC6310.15547233036022273No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTCTGCTTCGTATGC5620.13847139407677525No Hit
CGTCAGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCTTCT5330.1313260730301089No Hit
TGAATGATACGGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATG5250.12935494998275268No Hit
TGAATGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCT5190.1278766076972355No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTCTGCTTCGTATGCCG4900.12073128665056915No Hit
GAATCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCCTC4860.11974572512689105No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGT4750.11703543093677622No Hit
GAATAATACGGCGACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTA4720.11629625979401763No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCG4550.11210762331838565No Hit
AAAAAACTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATGCCGTCT4490.11062928103286848No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGTTCTGCTTCGTATTC4470.11013650027102942No Hit
GAATGATACGGCGACCCTGTCTCTTATACACATCTGACGCGTTCTGCTTCG4230.10422313112896071No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAGACGG302.162018E-645.0000042
CTACGGG302.162018E-645.0000043
CCCTTAA302.162018E-645.00000419
TGTAAGG302.162018E-645.0000042
TTTAGTG453.8380676E-1045.01
CTATGTC207.027889E-445.042
CAGAATA207.027889E-445.022
GTCTGGG351.2093369E-745.03
GAATAAC750.045.01
TCTAGTC207.027889E-445.043
CGTTATC207.027889E-445.01
CGTTAGG253.8863313E-545.02
ACGATAC253.8863313E-545.03
CTAGTCA207.027889E-445.044
CGCTATC900.045.01
CTAAACG207.027889E-445.01
TTGACGG253.8863313E-545.02
GTGTACG207.027889E-445.01
GCGATGT207.027889E-445.09
GCGATGA207.027889E-445.09
Produced by FastQC (version 0.11.5)