SRR2936787.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936787.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	338852
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTC	10871	3.208185284430961	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGC	9918	2.926941555605397	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCG	9685	2.858179972377321	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4639	1.3690342686482595	No Hit
GCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC	2747	0.8106784082726382	Illumina PCR Primer Index 10 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCT	2386	0.7041422213827866	No Hit
GAACTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCT	2130	0.6285930140592353	Illumina PCR Primer Index 10 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTC	1922	0.5672092831088499	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG	1835	0.5415343571824868	Illumina PCR Primer Index 10 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC	1765	0.5208763708049532	Illumina PCR Primer Index 10 (95% over 23bp)
GAATATGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTC	1601	0.4724776598633031	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCG	1557	0.45949263985456773	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTT	1532	0.45211478757687723	No Hit
CTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGCT	1526	0.4503441030302315	Illumina PCR Primer Index 10 (95% over 24bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGC	1472	0.4344079421104199	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCT	1380	0.40725744572851863	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTT	1310	0.3865994593509851	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTC	1208	0.3564978220580076	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTA	1174	0.34646394296034844	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTT	1092	0.32226458748952347	No Hit
CGTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCT	784	0.23136944742837579	Illumina PCR Primer Index 10 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	776	0.22900853469951482	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTC	706	0.2083505483219813	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCC	575	0.16969060238688277	No Hit
GATTCCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTT	550	0.16231275010919222	No Hit
TATTCCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTT	491	0.1449010187338425	No Hit
GCTGACTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC	491	0.1449010187338425	Illumina PCR Primer Index 10 (95% over 23bp)
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	441	0.1301453141784614	No Hit
AATGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTC	424	0.1251283746296318	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	415	0.12247234780966322	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATG	407	0.12011143508080224	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCG	401	0.1183407505341565	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGT	392	0.11568472371418789	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	373	0.11007755598314307	No Hit
TCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC	355	0.10476550234320588	Illumina PCR Primer Index 10 (95% over 23bp)
GAATAACTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCT	343	0.10122413324991442	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGGGA	65	0.0	45.000004	4
AATACGG	205	0.0	45.000004	5
TCACGCA	20	7.026239E-4	45.0	27
GTTGAGC	20	7.026239E-4	45.0	25
TGTCACG	20	7.026239E-4	45.0	25
ATGGGCG	35	1.2085366E-7	45.0	5
CGGGTCA	25	3.884965E-5	45.0	6
TAATACG	210	0.0	45.0	4
TACCCTA	20	7.026239E-4	45.0	31
GCCTTAT	25	3.884965E-5	45.0	25
TCGCAAG	20	7.026239E-4	45.0	1
CGCGGTA	20	7.026239E-4	45.0	31
ACTGAAC	20	7.026239E-4	45.0	42
GGCACAA	20	7.026239E-4	45.0	8
TTATGTC	35	1.2085366E-7	45.0	3
GGGTACG	20	7.026239E-4	45.0	7
GGCCGAT	35	1.2085366E-7	45.0	8
TCTTACA	20	7.026239E-4	45.0	32
TTCAACG	35	1.2085366E-7	45.0	18
TACGGGA	20	7.026239E-4	45.0	4

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content