##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936787.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	338852
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.6587448207477	31.0	31.0	34.0	30.0	34.0
2	31.6963659650821	31.0	31.0	34.0	30.0	34.0
3	31.407248592305784	31.0	31.0	34.0	28.0	34.0
4	35.368532574693376	37.0	35.0	37.0	33.0	37.0
5	30.886702749282872	35.0	35.0	37.0	0.0	37.0
6	33.10307449860116	36.0	35.0	37.0	17.0	37.0
7	34.89030314119439	37.0	35.0	37.0	32.0	37.0
8	35.15693577136921	36.0	35.0	37.0	32.0	37.0
9	37.2189333396291	39.0	37.0	39.0	34.0	39.0
10	37.18345472359614	39.0	37.0	39.0	34.0	39.0
11	37.02436461936185	39.0	37.0	39.0	33.0	39.0
12	36.9674135020599	39.0	37.0	39.0	33.0	39.0
13	36.78691287051574	39.0	35.0	39.0	33.0	39.0
14	38.05358386552241	40.0	37.0	41.0	33.0	41.0
15	37.93575661350678	40.0	37.0	41.0	33.0	41.0
16	37.96798012111482	40.0	37.0	41.0	33.0	41.0
17	37.84101023455668	40.0	36.0	41.0	33.0	41.0
18	37.633308937235135	39.0	36.0	41.0	32.0	41.0
19	37.50024199355471	39.0	36.0	41.0	32.0	41.0
20	37.472728506840745	39.0	35.0	41.0	32.0	41.0
21	37.497665647539336	39.0	35.0	41.0	33.0	41.0
22	37.54053687155454	39.0	35.0	41.0	33.0	41.0
23	37.56923671691476	39.0	35.0	41.0	33.0	41.0
24	37.407000696469254	39.0	35.0	41.0	33.0	41.0
25	37.285357619255606	39.0	35.0	41.0	33.0	41.0
26	37.15867989564766	39.0	35.0	41.0	32.0	41.0
27	37.12189097305018	39.0	35.0	40.0	32.0	41.0
28	37.04605845619917	39.0	35.0	40.0	32.0	41.0
29	36.85280299363734	39.0	35.0	40.0	31.0	41.0
30	36.69778251271942	39.0	35.0	40.0	31.0	41.0
31	36.458748952344976	38.0	35.0	40.0	30.0	41.0
32	36.19415260939879	38.0	35.0	40.0	30.0	41.0
33	36.13022794612397	38.0	35.0	40.0	30.0	41.0
34	35.77083800597311	38.0	35.0	40.0	29.0	41.0
35	35.73109204018274	38.0	35.0	40.0	28.0	41.0
36	35.442184198411105	38.0	35.0	40.0	26.0	41.0
37	35.25982434809297	38.0	34.0	40.0	25.0	41.0
38	35.04929585777862	38.0	34.0	40.0	25.0	41.0
39	34.71805094849669	38.0	33.0	40.0	23.0	41.0
40	34.69845537284714	38.0	33.0	40.0	24.0	41.0
41	34.58990355671503	38.0	33.0	40.0	23.0	41.0
42	34.649956323114516	38.0	33.0	40.0	23.0	41.0
43	34.56779360900924	38.0	33.0	40.0	23.0	41.0
44	34.35091426345425	37.0	33.0	40.0	22.0	41.0
45	34.34846481649806	37.0	33.0	40.0	22.0	41.0
46	33.911391994144935	37.0	33.0	40.0	21.0	41.0
47	33.78195495378513	37.0	33.0	40.0	21.0	41.0
48	33.53798708580737	37.0	32.0	40.0	20.0	41.0
49	33.41840980723148	37.0	32.0	40.0	19.0	41.0
50	33.18903533105899	37.0	32.0	40.0	18.0	41.0
51	30.699541392702418	35.0	27.0	38.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	4.0
9	11.0
10	19.0
11	12.0
12	11.0
13	5.0
14	10.0
15	7.0
16	19.0
17	56.0
18	122.0
19	293.0
20	519.0
21	811.0
22	1174.0
23	1755.0
24	2665.0
25	3887.0
26	5290.0
27	6134.0
28	6805.0
29	7717.0
30	9168.0
31	10988.0
32	13886.0
33	17901.0
34	25042.0
35	31659.0
36	31545.0
37	43437.0
38	57849.0
39	60041.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	48.109204018273466	17.039592506463	17.86384616292659	16.987357312336947
2	31.685219505860967	36.23499344846718	17.836695666544685	14.24309137912717
3	23.641589838631617	34.65967443013469	28.980203746768503	12.718531984465194
4	23.72540224050618	18.01464946348258	43.86074156268813	14.399206733323103
5	25.90865628652037	31.76312962591338	23.079397495071593	19.24881659249466
6	19.772939218301797	36.340939407174815	30.188105721672	13.698015652851392
7	58.94313741692538	6.210971161451017	27.713278953643478	7.13261246798012
8	54.67195117632476	14.984417975989517	21.27713574067735	9.06649510700837
9	50.31577207748516	7.03227367700353	20.552040418825918	22.0999138266854
10	33.52702654846363	18.546740169749626	29.689658021791228	18.23657525999551
11	25.50169395488296	20.438421493749484	31.373579025651317	22.68630552571624
12	23.260597547011674	16.878755326809344	37.32189864601655	22.538748480162432
13	18.892023656345543	20.26017258272048	43.93924191092276	16.908561850011214
14	17.12901207606861	29.314863126084546	31.526448124845064	22.029676673001784
15	13.085949027894184	24.59805460791142	44.85793207654079	17.458064287653606
16	14.496889497479726	27.64245157177765	32.047619609741126	25.8130393210015
17	14.606967053462869	25.423783834830544	34.05410031518185	25.915148796524733
18	14.62408367074711	29.06254057818753	33.08878212316882	23.224593627896546
19	15.659048788261543	29.714447605444267	29.67519743132695	24.951306174967243
20	17.58791448774096	28.064759836152657	34.957444548062284	19.389881128044102
21	17.649593332782455	32.579120087825956	31.926622832387004	17.844663747004592
22	14.80144723950279	23.937884386103665	31.24402393965507	30.01664443473847
23	15.609174506864354	30.833815353015474	32.63165039604311	20.92535974407706
24	22.179594631284456	22.04266169301052	28.913803076269286	26.863940599435743
25	18.183454723596142	33.0415638685916	26.727007661161807	22.047973746650452
26	15.927602611169478	23.384250351185766	31.52231652756956	29.165830510075196
27	21.273594371584053	26.18399773352378	26.65440959474933	25.887998300142833
28	16.34194279508458	21.715380165972164	35.81416075454771	26.12851628439555
29	20.51456092925525	19.80953336559914	34.75647185201799	24.91943385312762
30	17.826661787447026	22.922691912693445	34.16093161616281	25.08971468369672
31	25.921346192437994	20.16071913401721	28.063874493879332	25.85406017966546
32	21.388688867116027	23.593486241781072	34.0251791342533	20.992645756849598
33	17.35477435576594	19.197761854733038	33.35467991925679	30.09278387024424
34	19.35800880620448	17.725437654197112	35.928369907806356	26.98818363179205
35	14.486855618382066	17.58525846092099	36.466658009986666	31.46122791071028
36	20.13504420809085	18.83801777767285	33.340809556974726	27.686128457261578
37	16.423689398321393	19.19038400245535	37.76870137995349	26.61722521926977
38	19.403456376235052	21.5929078181625	32.87570974938912	26.12792605621333
39	20.64854272661811	18.09314981171721	34.546055505058256	26.712251956606426
40	18.35373555416524	17.973333490727516	42.57935617909884	21.093574776008406
41	15.753780411507087	23.491376766257837	29.808589000507595	30.94625382172748
42	17.4229457108118	20.005194008003492	39.88230850046628	22.689551780718425
43	22.630233848405794	18.84273960313057	29.272366696965047	29.254659851498587
44	21.312844545701367	18.65947375255274	32.36073566040631	27.66694604133958
45	19.1148347951318	17.654315158240173	30.6177918383247	32.61305820830333
46	26.51924734102204	21.534770342214298	28.323279779962935	23.622702536800727
47	16.85189994451855	18.365835231900654	41.20471474271953	23.57755008086126
48	19.303707813440678	20.748881517594704	31.341116475629477	28.606294193335142
49	16.795533153116995	16.991193795521347	39.87611110455302	26.337161946808635
50	18.961375467755833	16.14097009904029	37.12535266133887	27.772301771865003
51	17.496724233588708	16.524323303389092	31.383612904748976	34.59533955827323
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	437.0
1	731.5
2	1026.0
3	4575.5
4	8125.0
5	5368.5
6	2612.0
7	2186.5
8	1761.0
9	1685.0
10	1609.0
11	1652.5
12	1696.0
13	1661.5
14	1627.0
15	1600.0
16	1573.0
17	1536.5
18	1500.0
19	1408.5
20	1317.0
21	1415.0
22	1513.0
23	1491.5
24	1470.0
25	1625.0
26	2081.5
27	2383.0
28	2690.5
29	2998.0
30	3412.5
31	3827.0
32	4233.0
33	4639.0
34	5330.0
35	6021.0
36	6410.0
37	6799.0
38	7468.5
39	8138.0
40	9643.5
41	11149.0
42	12735.0
43	14321.0
44	21746.0
45	29171.0
46	51887.5
47	74604.0
48	56469.5
49	38335.0
50	36110.0
51	33885.0
52	25886.0
53	17887.0
54	14985.5
55	12084.0
56	10623.0
57	9162.0
58	8688.0
59	8214.0
60	7762.0
61	7310.0
62	6728.0
63	6146.0
64	5477.0
65	4808.0
66	3981.5
67	3155.0
68	2502.5
69	1850.0
70	1552.5
71	1255.0
72	1167.5
73	1080.0
74	933.5
75	622.0
76	457.0
77	307.0
78	157.0
79	115.5
80	74.0
81	63.0
82	52.0
83	44.0
84	36.0
85	24.0
86	12.0
87	9.0
88	6.0
89	4.0
90	2.0
91	1.0
92	0.0
93	0.5
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	338852.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	37.450108557895945
#Duplication Level	Percentage of deduplicated	Percentage of total
1	79.44753785731005	29.753189174138093
2	8.82961433448551	6.6134003070167315
3	3.2352689238653443	3.63483517238233
4	1.7156761539788088	2.5700903286679915
5	1.0850195612964	2.031705017899541
6	0.7524854048566891	1.690839606006917
7	0.5525992427697994	1.4486431141518061
8	0.4436338573670663	1.3291308894683806
9	0.3615328046860155	1.2185498504458687
>10	3.4100749836034563	23.633604873593622
>50	0.08840933690736767	2.2511430804273043
>100	0.05841331188522507	4.567913706994446
>500	0.003946845397650342	1.0024459863071555
>1k	0.013419274352011165	9.245797896709702
>5k	0.0015787381590601371	5.795030571978523
>10k+	7.893690795300686E-4	3.213680423811586
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTC	10871	3.208185284430961	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGC	9918	2.926941555605397	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCG	9685	2.858179972377321	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	4639	1.3690342686482595	No Hit
GCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC	2747	0.8106784082726382	Illumina PCR Primer Index 10 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCT	2386	0.7041422213827866	No Hit
GAACTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCT	2130	0.6285930140592353	Illumina PCR Primer Index 10 (95% over 21bp)
GAATGATCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTC	1922	0.5672092831088499	No Hit
CGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTG	1835	0.5415343571824868	Illumina PCR Primer Index 10 (95% over 22bp)
CCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC	1765	0.5208763708049532	Illumina PCR Primer Index 10 (95% over 23bp)
GAATATGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTC	1601	0.4724776598633031	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCG	1557	0.45949263985456773	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTT	1532	0.45211478757687723	No Hit
CTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGCT	1526	0.4503441030302315	Illumina PCR Primer Index 10 (95% over 24bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGC	1472	0.4344079421104199	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCT	1380	0.40725744572851863	No Hit
GAATGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTT	1310	0.3865994593509851	No Hit
CGTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTC	1208	0.3564978220580076	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTA	1174	0.34646394296034844	No Hit
GAATCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTT	1092	0.32226458748952347	No Hit
CGTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCT	784	0.23136944742837579	Illumina PCR Primer Index 10 (95% over 21bp)
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	776	0.22900853469951482	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTC	706	0.2083505483219813	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCC	575	0.16969060238688277	No Hit
GATTCCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTT	550	0.16231275010919222	No Hit
TATTCCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTT	491	0.1449010187338425	No Hit
GCTGACTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC	491	0.1449010187338425	Illumina PCR Primer Index 10 (95% over 23bp)
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	441	0.1301453141784614	No Hit
AATGCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTC	424	0.1251283746296318	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	415	0.12247234780966322	No Hit
GAATGATACGGCCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATG	407	0.12011143508080224	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCG	401	0.1183407505341565	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGT	392	0.11568472371418789	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	373	0.11007755598314307	No Hit
TCTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCTTCTGC	355	0.10476550234320588	Illumina PCR Primer Index 10 (95% over 23bp)
GAATAACTGTCTCTTATACACATCTGACGCTCGTTCTCTCGTATGCCGTCT	343	0.10122413324991442	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.5863916990308453	0.0
2	0.0	0.0	0.0	2.6554365917863847	0.0
3	0.0	0.0	0.0	3.8353027280346583	0.0
4	0.0	0.0	0.0	5.5085996246148765	0.0
5	0.0	0.0	0.0	11.740228772443427	0.0
6	0.0	0.0	0.0	14.761016609021048	0.0
7	0.0	0.0	0.0	17.50233140131975	0.0
8	0.0	0.0	0.0	19.992799216176973	0.0
9	0.0	0.0	0.0	20.914440522706077	0.0
10	0.0	0.0	0.0	25.894195696056094	0.0
11	0.0	0.0	0.0	28.065350064334872	0.0
12	0.0	0.0	0.0	33.17672612231889	0.0
13	0.0	0.0	0.0	34.041705523355326	0.0
14	0.0	0.0	0.0	34.47375255273689	0.0
15	0.0	0.0	0.0	35.38595020835055	0.0
16	0.0	0.0	0.0	36.37841889674548	0.0
17	0.0	0.0	0.0	37.32514490101873	0.0
18	2.951140911076222E-4	0.0	0.0	38.40880384356592	0.0
19	2.951140911076222E-4	0.0	0.0	39.10940469585542	0.0
20	2.951140911076222E-4	0.0	0.0	39.750392501741175	0.0
21	2.951140911076222E-4	0.0	0.0	40.43446696492864	0.0
22	2.951140911076222E-4	0.0	0.0	41.20796099772172	0.0
23	2.951140911076222E-4	0.0	0.0	41.91889084319998	0.0
24	2.951140911076222E-4	0.0	0.0	42.509119025415224	0.0
25	2.951140911076222E-4	0.0	0.0	42.99045010801176	0.0
26	2.951140911076222E-4	0.0	0.0	43.449057405593	0.0
27	2.951140911076222E-4	0.0	0.0	43.94514419274491	0.0
28	2.951140911076222E-4	0.0	0.0	44.429131302161416	0.0
29	2.951140911076222E-4	0.0	0.0	44.87209755291396	0.0
30	2.951140911076222E-4	0.0	0.0	45.362577172334824	0.0
31	2.951140911076222E-4	0.0	0.0	45.81351150354727	0.0
32	2.951140911076222E-4	0.0	0.0	46.26149469384864	0.0
33	2.951140911076222E-4	0.0	0.0	46.696787978232386	0.0
34	2.951140911076222E-4	0.0	0.0	47.10729167896309	0.0
35	2.951140911076222E-4	0.0	0.0	47.59068856019737	0.0
36	2.951140911076222E-4	0.0	0.0	47.99617532137925	0.0
37	2.951140911076222E-4	0.0	0.0	48.398415827558935	0.0
38	2.951140911076222E-4	0.0	0.0	48.79947587737419	0.0
39	2.951140911076222E-4	0.0	0.0	49.1984701285517	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
AACGGGA	65	0.0	45.000004	4
AATACGG	205	0.0	45.000004	5
TCACGCA	20	7.026239E-4	45.0	27
GTTGAGC	20	7.026239E-4	45.0	25
TGTCACG	20	7.026239E-4	45.0	25
ATGGGCG	35	1.2085366E-7	45.0	5
CGGGTCA	25	3.884965E-5	45.0	6
TAATACG	210	0.0	45.0	4
TACCCTA	20	7.026239E-4	45.0	31
GCCTTAT	25	3.884965E-5	45.0	25
TCGCAAG	20	7.026239E-4	45.0	1
CGCGGTA	20	7.026239E-4	45.0	31
ACTGAAC	20	7.026239E-4	45.0	42
GGCACAA	20	7.026239E-4	45.0	8
TTATGTC	35	1.2085366E-7	45.0	3
GGGTACG	20	7.026239E-4	45.0	7
GGCCGAT	35	1.2085366E-7	45.0	8
TCTTACA	20	7.026239E-4	45.0	32
TTCAACG	35	1.2085366E-7	45.0	18
TACGGGA	20	7.026239E-4	45.0	4
>>END_MODULE