SRR2936785.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936785.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	108135
Sequences flagged as poor quality	0
Sequence length	51
%GC	47

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTC	4108	3.7989550099412774	Illumina PCR Primer Index 9 (95% over 21bp)
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGC	2237	2.0687104082859387	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCG	2069	1.9133490544227125	No Hit
GAATGCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTT	1270	1.1744578536089147	No Hit
GAACTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTCT	887	0.8202709575992971	Illumina PCR Primer Index 9 (95% over 22bp)
GCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTCTGC	725	0.6704582235169002	Illumina PCR Primer Index 9 (95% over 24bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	638	0.5900032366948722	No Hit
GAATGACTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCT	629	0.5816803070236278	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTC	627	0.5798307670966847	No Hit
GAATATGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTC	592	0.5474638183751792	Illumina PCR Primer Index 9 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTCTGCT	375	0.3467887363018449	Illumina PCR Primer Index 9 (96% over 25bp)
GAATAATACCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCG	342	0.3162713275072826	No Hit
CCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTCTGC	327	0.3023997780552088	Illumina PCR Primer Index 9 (95% over 24bp)
CGCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTCTG	325	0.3005502381282656	Illumina PCR Primer Index 9 (95% over 23bp)
GAATAATACGGCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGC	316	0.29222730845702133	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	258	0.2385906505756693	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCAGCAGAACTCGTA	233	0.2154714014888796	No Hit
GAATACTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTT	194	0.1794053729134878	No Hit
GAATCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTT	193	0.17848060295001617	No Hit
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	183	0.1692329033153003	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	167	0.15443658389975495	No Hit
GCTGACTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTCTGC	142	0.13131733481296526	Illumina PCR Primer Index 9 (95% over 24bp)
GATACTGAAGCTACGAATATACTGACTATGAAGACCTATGCTTTGATTCAT	135	0.12484394506866417	No Hit
AATGCTGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTC	135	0.12484394506866417	Illumina PCR Primer Index 9 (95% over 21bp)
GAACAGTCTCTTATACACATCTGACGCAGCAGAACTCGTATGCCGTCTTCT	123	0.11374670550700512	Illumina PCR Primer Index 9 (95% over 22bp)
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	115	0.10634854579923245	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ATGGGAC	50	2.1827873E-11	45.0	5
TTAGGAT	20	7.0049317E-4	45.0	3
TAATACG	55	1.8189894E-12	45.0	4
TAATACC	25	3.8673214E-5	45.0	4
CTATATA	35	1.198332E-7	45.0	18
GGACTTG	20	7.0049317E-4	45.0	27
ATAATAC	80	0.0	45.0	3
GCAGTGC	20	7.0049317E-4	45.0	13
TCGGGAG	20	7.0049317E-4	45.0	5
ATGTCTC	40	6.717528E-9	45.0	5
TACGGGA	25	3.8673214E-5	45.0	4
TCGATCA	20	7.0049317E-4	45.0	17
ACGGGTA	20	7.0049317E-4	45.0	5
CCAGACG	20	7.0049317E-4	45.0	1
TATTGCA	20	7.0049317E-4	45.0	23
TGCATAC	20	7.0049317E-4	45.0	26
GCATTTA	40	6.717528E-9	45.0	4
ACTAGGG	35	1.198332E-7	45.0	3
TATCTAT	35	1.198332E-7	45.0	15
CCATAGG	20	7.0049317E-4	45.0	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content