FastQCFastQC Report
Sat 14 Jan 2017
SRR2936780.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936780.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences179833
Sequences flagged as poor quality0
Sequence length51
%GC43

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTC60593.369237014341083No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGC40732.264879082259652No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCG39162.1775758620497907No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT24921.385730094031685No Hit
CCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC17120.9519943503139023No Hit
CTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGCT14870.8268782703953111No Hit
GCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC14220.7907336250854959No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTT13940.7751636240289602No Hit
CGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTG13500.7506964795115468No Hit
GAACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCT11430.6355896859864429No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCT11380.6328093286549187No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTC10730.5966646833451036No Hit
GAATATGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTC9140.5082493202026324No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCT7900.4392964583808311No Hit
CGTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCT7340.4081564562677596No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA7230.4020396701384062No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTT7190.39981538427318675No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTC7120.3959228840090529No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCG6850.3809089544188219No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGC6250.34754466644053095No Hit
AATGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTC5900.3280821651198612No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCC5390.29972252033831387No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTC4800.26691430382632775No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTA4510.25078823130348715No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT4270.23744251611217074No Hit
AAAACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTC3710.20630251399909916No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCC3430.19073251294256338No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCG3160.17571858335233245No Hit
AATCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCT3060.17015786868928395No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTT2940.16348501109362576No Hit
TCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC2800.15570001056535784No Hit
CCTGACTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC2770.1540317961664433No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCT2570.1429103668403463No Hit
CTGTATCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGCT2530.14068608097512694No Hit
ACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC2490.13846179510990753No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGT2450.1362375092446881No Hit
GCTGACTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC2370.13178893751424933No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT2360.13123286604794449No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGT2320.1290085801827251No Hit
CGCTATCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTG2060.114550722058799No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA2030.11288250765988445No Hit
GAATACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTT1980.11010215032836021No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTT1940.1078778644631408No Hit
GAATAACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCT1940.1078778644631408No Hit

[FAIL]Adapter Content

Adapter graph

[FAIL]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TAATACG1150.045.0000044
GGGACCG551.8189894E-1245.0000047
CGCTATC351.2042983E-745.0000041
GAGCATG351.2042983E-745.0000041
TGGGACC351.2042983E-745.0000046
TCACGGG406.7593646E-945.03
GTTGACG207.017403E-445.01
CATGGGT207.017403E-445.04
GGGTGGA207.017403E-445.07
CTCACGG207.017403E-445.02
ACACGCG207.017403E-445.036
CGGCAGG207.017403E-445.02
AAGACGG406.7593646E-945.02
ACGGGGC253.8776452E-545.05
CGTTATT2100.045.01
CGTTATC207.017403E-445.01
CCATCTA207.017403E-445.013
GGAACAC406.7593646E-945.08
CGAATGG207.017403E-445.02
GCGATAA207.017403E-445.09
Produced by FastQC (version 0.11.5)