##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936780.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	179833
Sequences flagged as poor quality	0
Sequence length	51
%GC	43
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.5641901097129	31.0	31.0	34.0	30.0	34.0
2	31.603159598071546	31.0	31.0	34.0	30.0	34.0
3	31.28727764092241	31.0	31.0	34.0	28.0	34.0
4	35.29125355190649	37.0	35.0	37.0	33.0	37.0
5	30.721925341844933	35.0	35.0	37.0	0.0	37.0
6	33.000216867871856	36.0	35.0	37.0	17.0	37.0
7	34.827250838277735	37.0	35.0	37.0	32.0	37.0
8	35.165948407689356	37.0	35.0	37.0	32.0	37.0
9	37.2215166293172	39.0	37.0	39.0	34.0	39.0
10	37.16662125416359	39.0	37.0	39.0	34.0	39.0
11	37.03090089138256	39.0	37.0	39.0	33.0	39.0
12	37.12655074430166	39.0	37.0	39.0	33.0	39.0
13	37.05051353199913	39.0	37.0	39.0	33.0	39.0
14	38.25150556349502	40.0	37.0	41.0	34.0	41.0
15	38.18372045175246	40.0	37.0	41.0	33.0	41.0
16	38.1532866604016	40.0	37.0	41.0	33.0	41.0
17	38.07104925124977	40.0	37.0	41.0	33.0	41.0
18	37.83081525637675	39.0	37.0	41.0	33.0	41.0
19	37.68824409313085	39.0	37.0	41.0	33.0	41.0
20	37.61249047727614	39.0	36.0	41.0	33.0	41.0
21	37.58831805063587	39.0	36.0	41.0	33.0	41.0
22	37.619869545634	39.0	36.0	41.0	33.0	41.0
23	37.60855905200936	39.0	36.0	41.0	33.0	41.0
24	37.50918352026603	39.0	35.0	41.0	33.0	41.0
25	37.322504768312825	39.0	35.0	41.0	32.0	41.0
26	37.25298471359539	39.0	35.0	41.0	32.0	41.0
27	37.2110124393187	39.0	35.0	41.0	32.0	41.0
28	37.11140891827418	39.0	35.0	41.0	32.0	41.0
29	37.04142176352505	39.0	35.0	41.0	32.0	41.0
30	36.96438362258318	39.0	35.0	41.0	31.0	41.0
31	36.71272235907759	39.0	35.0	41.0	31.0	41.0
32	36.46507037084406	39.0	35.0	40.0	30.0	41.0
33	36.313190571252214	39.0	35.0	40.0	30.0	41.0
34	35.97413711610216	39.0	35.0	40.0	28.0	41.0
35	35.591070604394076	39.0	35.0	40.0	25.0	41.0
36	35.368124871408476	38.0	35.0	40.0	24.0	41.0
37	35.327231375776414	38.0	35.0	40.0	24.0	41.0
38	35.11410586488576	38.0	34.0	40.0	24.0	41.0
39	34.98639293121952	38.0	34.0	40.0	24.0	41.0
40	34.756257194174594	38.0	34.0	40.0	23.0	41.0
41	34.56981199223724	38.0	33.0	40.0	22.0	41.0
42	34.57642924268627	38.0	34.0	40.0	22.0	41.0
43	34.506175173633316	38.0	33.0	40.0	21.0	41.0
44	34.40762818837477	38.0	33.0	40.0	21.0	41.0
45	34.33754650147637	38.0	33.0	40.0	20.0	41.0
46	34.01174422936836	38.0	33.0	40.0	19.0	41.0
47	33.807971840540944	38.0	32.0	40.0	19.0	41.0
48	33.55657193062452	38.0	32.0	40.0	17.0	41.0
49	33.34973558801777	38.0	32.0	40.0	14.0	41.0
50	33.08974437394694	37.0	31.0	40.0	13.0	41.0
51	30.894802400004448	35.0	27.0	39.0	8.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	3.0
9	3.0
10	7.0
11	5.0
12	6.0
13	7.0
14	1.0
15	13.0
16	15.0
17	35.0
18	75.0
19	151.0
20	380.0
21	487.0
22	858.0
23	1172.0
24	1810.0
25	2447.0
26	3273.0
27	3534.0
28	3661.0
29	3875.0
30	4383.0
31	5390.0
32	6636.0
33	8396.0
34	11050.0
35	14436.0
36	16172.0
37	22180.0
38	32266.0
39	37092.0
40	13.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	41.53853853297226	19.911250993977745	18.934789499146433	19.615420973903568
2	29.52183414612446	38.07254508349413	18.46435303865253	13.941267731728882
3	21.486601458019386	34.23676410892328	32.48847541886083	11.788159014196504
4	21.09290285987555	20.06417064721158	44.986181624062326	13.856744868850546
5	23.519598738829913	33.34871797723443	25.636562811052478	17.495120472883173
6	18.192434091629455	33.692370143410834	33.50997870246284	14.605217062496873
7	57.264239600073395	7.357937642145769	27.72516723849349	7.65265551928734
8	54.43439190804802	13.039319813382416	22.36463830331474	10.16164997525482
9	50.39453270534329	8.151451624562789	22.29957794175707	19.154437728336845
10	25.625997453192685	30.943152813999653	28.450840502021318	14.98000923078634
11	18.773528773918024	22.98465798824465	37.11387787558457	21.127935362252757
12	16.830058999182576	20.610232827122942	41.50128174472984	21.05842642896465
13	15.901975721919781	23.13146085534913	45.58896309353678	15.377600329194308
14	14.760361001595927	31.52702785361975	33.2180411826528	20.494569962131536
15	10.929584670221818	28.42637335750391	43.524269739146874	17.1197722331274
16	12.972591237425835	32.87216473061118	30.439352065527466	23.715891966435525
17	13.316799475068535	29.27327020068619	33.83583658171748	23.57409374252779
18	13.5536859197144	30.45770242391552	33.861971940633815	22.126639715736264
19	12.818559441259392	30.748527800792957	31.238982834073838	25.193929923873814
20	14.326625257878142	31.901820021909217	35.77207742739097	17.999477292821673
21	16.148871452959135	32.992276167333024	34.37856233283102	16.480290046876824
22	13.78167522089939	26.07641534089961	32.9316643775058	27.210245060695197
23	14.029127023405048	30.83249459220499	34.08106409835792	21.057314286032042
24	20.946656064237377	24.85361418649525	29.120906618918664	25.07882313034871
25	17.386130465487422	34.90293772555649	26.408390006283604	21.30254180267248
26	18.760739130193013	26.004682121746285	28.42136871430716	26.81321003375354
27	21.789660407155527	26.77094860231437	26.46788965317823	24.971501337351874
28	16.13441359483521	23.4834540935201	33.85752336890337	26.524608942741324
29	24.15407628188375	20.462873888552156	26.506814655819568	28.87623517374453
30	18.41041410642096	23.31996908242647	29.499591287472267	28.770025523680303
31	18.954808071933403	22.151662931719983	32.72981043523714	26.163718561109473
32	18.022832294406477	26.6252578781425	27.464369720796515	27.887540106654505
33	13.690479500425395	21.1329400054495	30.790233160765823	34.38634733335928
34	18.725706627815807	21.21079001073218	36.48051247546334	23.582990885988668
35	14.564067773990313	26.089761056090925	29.167060550621965	30.17911061929679
36	18.905873782898578	22.069364354706867	37.91795721586138	21.106804646533174
37	14.099192028159461	22.71774368441832	38.4245383216651	24.75852596575712
38	14.036912023933315	24.729610249509268	33.31869011805397	27.914787608503445
39	20.650826044163196	20.363893167549893	35.14872131366323	23.836559474623677
40	19.002074146569317	23.68419589285615	34.524809128469194	22.78892083210534
41	16.4319118293083	26.372245360973796	35.04918452119467	22.14665828852324
42	18.057864796783683	24.879749545411574	36.457157473878546	20.605228183926197
43	23.23933871981227	20.613569255920773	33.27086797195176	22.8762240523152
44	20.22598744390629	20.085857434397468	31.852885732874388	27.835269388821853
45	18.51495554208627	18.13793908793158	30.238054194725105	33.109051175257044
46	25.577619235624162	21.280298944020284	29.694772372145266	23.447309448210284
47	16.062680375681882	19.15276951393793	41.427880311177596	23.356669799202592
48	17.825426923868257	21.562783248903152	32.41729827117381	28.194491556054786
49	17.294378673547126	17.334415819121073	40.96745313707718	24.403752370254626
50	18.934233427680127	16.376860754144122	37.748355418638404	26.94055039953735
51	17.250449027709042	16.81448899812604	32.02137538716476	33.913686587000164
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	932.0
1	1000.5
2	1069.0
3	2774.0
4	4479.0
5	3036.0
6	1593.0
7	1396.0
8	1199.0
9	1214.5
10	1230.0
11	1223.5
12	1217.0
13	1189.5
14	1162.0
15	1092.0
16	1022.0
17	979.5
18	937.0
19	907.5
20	878.0
21	904.0
22	930.0
23	908.5
24	887.0
25	968.0
26	1184.5
27	1320.0
28	1511.5
29	1703.0
30	1919.0
31	2135.0
32	2476.0
33	2817.0
34	3272.0
35	3727.0
36	4189.0
37	4651.0
38	5141.5
39	5632.0
40	6254.5
41	6877.0
42	8242.5
43	9608.0
44	13911.0
45	18214.0
46	28997.5
47	39781.0
48	31086.5
49	22392.0
50	20537.0
51	18682.0
52	13784.5
53	8887.0
54	7113.5
55	5340.0
56	4379.0
57	3418.0
58	2866.0
59	2314.0
60	1862.0
61	1410.0
62	1210.5
63	1011.0
64	799.5
65	588.0
66	450.0
67	312.0
68	260.5
69	209.0
70	157.0
71	105.0
72	79.5
73	54.0
74	37.5
75	19.0
76	17.0
77	13.5
78	10.0
79	7.0
80	4.0
81	2.5
82	1.0
83	1.5
84	2.0
85	2.5
86	3.0
87	3.0
88	3.0
89	2.0
90	1.0
91	0.5
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	179833.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	48.32094220749251
#Duplication Level	Percentage of deduplicated	Percentage of total
1	80.28240330506232	38.79321370382522
2	9.014120165253116	8.711415591131773
3	3.9506542228155173	5.726980031473645
4	2.194552170961023	4.241713144973392
5	1.4925716653049013	3.606123458986949
6	0.8953128416401026	2.5957416047110375
7	0.6444411199466035	2.1798001479150098
8	0.3613473422557741	1.3968515233577818
9	0.2508717216934992	1.0910122168901148
>10	0.8009482490764929	6.230780779945839
>50	0.03797599456828199	1.2689550861076666
>100	0.049483871710185624	5.089722131088288
>500	0.011507877141903633	3.9097384795893966
>1k	0.012658664856093996	11.78871508566281
>5k	0.0011507877141903634	3.369237014341083
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTC	6059	3.369237014341083	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGC	4073	2.264879082259652	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCG	3916	2.1775758620497907	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2492	1.385730094031685	No Hit
CCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	1712	0.9519943503139023	No Hit
CTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGCT	1487	0.8268782703953111	No Hit
GCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	1422	0.7907336250854959	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTT	1394	0.7751636240289602	No Hit
CGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTG	1350	0.7506964795115468	No Hit
GAACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCT	1143	0.6355896859864429	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCT	1138	0.6328093286549187	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTC	1073	0.5966646833451036	No Hit
GAATATGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTC	914	0.5082493202026324	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCT	790	0.4392964583808311	No Hit
CGTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCT	734	0.4081564562677596	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	723	0.4020396701384062	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTT	719	0.39981538427318675	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTC	712	0.3959228840090529	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCG	685	0.3809089544188219	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGC	625	0.34754466644053095	No Hit
AATGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTC	590	0.3280821651198612	No Hit
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCC	539	0.29972252033831387	No Hit
CGTTTTTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTC	480	0.26691430382632775	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTA	451	0.25078823130348715	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	427	0.23744251611217074	No Hit
AAAACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTC	371	0.20630251399909916	No Hit
AATGATACGGCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCC	343	0.19073251294256338	No Hit
CGTTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCG	316	0.17571858335233245	No Hit
AATCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCT	306	0.17015786868928395	No Hit
GAATCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTT	294	0.16348501109362576	No Hit
TCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	280	0.15570001056535784	No Hit
CCTGACTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	277	0.1540317961664433	No Hit
AATGATCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCT	257	0.1429103668403463	No Hit
CTGTATCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGCT	253	0.14068608097512694	No Hit
ACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	249	0.13846179510990753	No Hit
CGTTTTTTCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGT	245	0.1362375092446881	No Hit
GCTGACTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTGC	237	0.13178893751424933	No Hit
CGGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	236	0.13123286604794449	No Hit
AATGATACCTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGT	232	0.1290085801827251	No Hit
CGCTATCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTTCTG	206	0.114550722058799	No Hit
GAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	203	0.11288250765988445	No Hit
GAATACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTT	198	0.11010215032836021	No Hit
AAAAACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCTT	194	0.1078778644631408	No Hit
GAATAACTGTCTCTTATACACATCTGACGCGCTCCTATTCGTATGCCGTCT	194	0.1078778644631408	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	1.053199357181385	0.0
2	0.0	0.0	0.0	3.821879187913231	0.0
3	0.0	0.0	0.0	5.304921788548264	0.0
4	0.0	0.0	0.0	7.3406994266903185	0.0
5	0.0	0.0	0.0	13.971295590909344	0.0
6	0.0	0.0	0.0	17.411709752937448	0.0
7	0.0	0.0	0.0	20.313290664116153	0.0
8	0.0	0.0	0.0	23.125344069219775	0.0
9	0.0	0.0	0.0	24.28975771966213	0.0
10	0.0	0.0	0.0	28.512008363314852	0.0
11	0.0	0.0	0.0	30.747415657860348	0.0
12	0.0	0.0	0.0	35.1909827451024	0.0
13	0.0	0.0	0.0	36.13908459515217	0.0
14	0.0	0.0	0.0	36.59339498312323	0.0
15	0.0	0.0	0.0	37.36299789248914	0.0
16	0.0	0.0	0.0	38.19654902048011	0.0
17	0.0	0.0	0.0	39.13742194146792	0.0
18	0.0	0.0	0.0	40.18617272691886	0.0
19	0.0	0.0	0.0	40.89405170352494	0.0
20	0.0	0.0	0.0	41.51351531698854	0.0
21	0.0	0.0	0.0	42.12908643018801	0.0
22	0.0	0.0	0.0	42.789143260691866	0.0
23	0.0	0.0	0.0	43.42862544694244	0.0
24	0.0	0.0	0.0	43.95522512553313	0.0
25	0.0	0.0	0.0	44.40008229857701	0.0
26	0.0	0.0	0.0	44.8805280454644	0.0
27	0.0	0.0	0.0	45.34317950543004	0.0
28	0.0	0.0	0.0	45.777471320614126	0.0
29	0.0	0.0	0.0	46.229001351253665	0.0
30	0.0	0.0	0.0	46.749484243715	0.0
31	0.0	0.0	0.0	47.197121774090405	0.0
32	0.0	0.0	0.0	47.60249787302664	0.0
33	0.0	0.0	0.0	47.98618718477699	0.0
34	0.0	0.0	0.0	48.38099792585343	0.0
35	0.0	0.0	0.0	48.79249081091902	0.0
36	0.0	0.0	0.0	49.155605478416085	0.0
37	0.0	0.0	0.0	49.52706121790773	0.0
38	5.560714663048495E-4	0.0	0.0	49.927988745113524	0.0
39	5.560714663048495E-4	0.0	0.0	50.32057520032475	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	115	0.0	45.000004	4
GGGACCG	55	1.8189894E-12	45.000004	7
CGCTATC	35	1.2042983E-7	45.000004	1
GAGCATG	35	1.2042983E-7	45.000004	1
TGGGACC	35	1.2042983E-7	45.000004	6
TCACGGG	40	6.7593646E-9	45.0	3
GTTGACG	20	7.017403E-4	45.0	1
CATGGGT	20	7.017403E-4	45.0	4
GGGTGGA	20	7.017403E-4	45.0	7
CTCACGG	20	7.017403E-4	45.0	2
ACACGCG	20	7.017403E-4	45.0	36
CGGCAGG	20	7.017403E-4	45.0	2
AAGACGG	40	6.7593646E-9	45.0	2
ACGGGGC	25	3.8776452E-5	45.0	5
CGTTATT	210	0.0	45.0	1
CGTTATC	20	7.017403E-4	45.0	1
CCATCTA	20	7.017403E-4	45.0	13
GGAACAC	40	6.7593646E-9	45.0	8
CGAATGG	20	7.017403E-4	45.0	2
GCGATAA	20	7.017403E-4	45.0	9
>>END_MODULE