##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936778.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	536941
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.899478713676178	33.0	31.0	34.0	30.0	34.0
2	31.976872691785502	33.0	31.0	34.0	30.0	34.0
3	31.862096580443662	33.0	31.0	34.0	30.0	34.0
4	35.62612838282046	37.0	35.0	37.0	33.0	37.0
5	30.920136476819614	37.0	35.0	37.0	0.0	37.0
6	33.22046556325555	37.0	35.0	37.0	17.0	37.0
7	35.128321361192384	37.0	35.0	37.0	32.0	37.0
8	35.50956995275086	37.0	35.0	37.0	33.0	37.0
9	37.5532395551839	39.0	37.0	39.0	35.0	39.0
10	37.27621656755584	39.0	37.0	39.0	34.0	39.0
11	37.10892258181067	39.0	37.0	39.0	34.0	39.0
12	37.10435224726739	39.0	37.0	39.0	33.0	39.0
13	37.0080586135162	39.0	37.0	39.0	33.0	39.0
14	38.25930037005928	40.0	37.0	41.0	34.0	41.0
15	38.227877923272764	40.0	37.0	41.0	33.0	41.0
16	38.22590005233349	40.0	37.0	41.0	34.0	41.0
17	38.1662603526272	40.0	37.0	41.0	33.0	41.0
18	38.06644305426481	40.0	37.0	41.0	33.0	41.0
19	37.99254107993243	40.0	37.0	41.0	33.0	41.0
20	37.99768503429613	40.0	37.0	41.0	33.0	41.0
21	38.02137292551696	40.0	37.0	41.0	33.0	41.0
22	38.05058656351443	40.0	37.0	41.0	34.0	41.0
23	38.08165515391821	40.0	37.0	41.0	34.0	41.0
24	38.021683946653354	40.0	37.0	41.0	34.0	41.0
25	37.90779433867036	40.0	37.0	41.0	33.0	41.0
26	37.82569779547474	40.0	36.0	41.0	33.0	41.0
27	37.83228324899756	40.0	37.0	41.0	33.0	41.0
28	37.816320601332365	40.0	36.0	41.0	33.0	41.0
29	37.78258877604802	40.0	36.0	41.0	33.0	41.0
30	37.722014150530505	40.0	36.0	41.0	33.0	41.0
31	37.701645059699295	40.0	36.0	41.0	33.0	41.0
32	37.58102473083635	40.0	36.0	41.0	33.0	41.0
33	37.49905296857569	40.0	36.0	41.0	33.0	41.0
34	37.36630467779514	40.0	36.0	41.0	32.0	41.0
35	37.27232042254177	40.0	36.0	41.0	32.0	41.0
36	37.15208933569983	40.0	36.0	41.0	32.0	41.0
37	37.06900944424061	40.0	36.0	41.0	31.0	41.0
38	37.03107604001185	39.0	35.0	41.0	31.0	41.0
39	36.906149837691665	39.0	35.0	41.0	31.0	41.0
40	36.79375015132016	39.0	35.0	41.0	31.0	41.0
41	36.752386202580915	39.0	35.0	41.0	31.0	41.0
42	36.6856172279636	39.0	35.0	41.0	30.0	41.0
43	36.629417384777845	39.0	35.0	41.0	30.0	41.0
44	36.51398757032896	39.0	35.0	41.0	30.0	41.0
45	36.46989334023664	39.0	35.0	41.0	30.0	41.0
46	36.27379544493715	39.0	35.0	40.0	30.0	41.0
47	36.1265744281029	39.0	35.0	40.0	30.0	41.0
48	36.00802322787792	39.0	35.0	40.0	29.0	41.0
49	35.912422407676075	39.0	35.0	40.0	28.0	41.0
50	35.73616840583975	38.0	35.0	40.0	27.0	41.0
51	33.79021903710091	37.0	32.0	40.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	10.0
9	16.0
10	18.0
11	22.0
12	9.0
13	10.0
14	12.0
15	16.0
16	29.0
17	52.0
18	140.0
19	262.0
20	436.0
21	638.0
22	985.0
23	1520.0
24	2129.0
25	3174.0
26	4148.0
27	5113.0
28	5765.0
29	7230.0
30	9162.0
31	12108.0
32	15824.0
33	22421.0
34	33197.0
35	42218.0
36	45592.0
37	69200.0
38	108377.0
39	147070.0
40	37.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.88966199265841	20.65534202081793	23.882325991123793	10.572669995399867
2	30.724790991933936	30.046317938097484	24.000402278835104	15.228488791133476
3	29.06017607148644	29.965862171076523	26.942811221344616	14.03115053609242
4	26.17717775323546	24.471217508068857	32.7004270487819	16.65117768991379
5	23.85513492171393	37.46091283772333	22.00297611841897	16.680976122143772
6	24.333958479609493	36.950428445583405	25.602626731801077	13.112986343006028
7	75.45354145055043	5.6631920453085165	14.240857002910934	4.642409501230117
8	75.38872986045023	8.616216679299962	10.828005311570545	5.167048148679278
9	69.26924932162007	7.374553256316801	11.362514689695887	11.993682732367244
10	36.56770483162954	26.556549043563443	20.885907390197435	15.989838734609577
11	27.355147027327025	24.68744238193768	27.305607133744676	20.65180345699062
12	24.30118020415651	21.365289668697304	32.66802125373179	21.665508873414396
13	21.450587680955636	23.2738792530278	36.620038328233456	18.65549473778311
14	20.19681864487905	28.69589023747488	29.886337605062753	21.220953512583318
15	18.05263520573024	25.74677664771362	38.184642260509065	18.01594588604707
16	19.683540649717568	27.83285314401396	29.82152601496254	22.662080191305936
17	20.511750825509694	25.755716177382617	30.830202945947505	22.902330051160185
18	19.98562225644903	27.72725494979895	31.356517755209605	20.93060503854241
19	19.956755025226236	28.956067798882934	28.870583546423163	22.21659362946767
20	20.988153260786564	28.343710016556756	31.97260034156453	18.69553638109215
21	20.79576713270173	29.863988780890267	30.597216453949315	18.743027632458688
22	19.285731579447276	25.015970097273254	29.592078086791656	26.106220236487808
23	19.024436576830603	29.375108252117087	30.82834054393313	20.77211462711918
24	21.86385468794523	24.57625698167955	29.30470945597375	24.25517887440147
25	19.065781901549705	31.25930037005928	27.647357903382307	22.02755982500871
26	18.34857088581427	27.132962467012206	30.229764536513322	24.288702110660203
27	22.56858761018436	27.944224784473526	27.406549322923745	22.080638282418366
28	17.243421530484724	27.01414121849514	31.677037141883375	24.06540010913676
29	21.148692314425606	24.895658927144694	30.66947765210703	23.286171106322666
30	22.704542957233663	26.15259404664572	30.063452036629723	21.079410959490893
31	21.626212190911108	25.597225765959386	27.353098385111213	25.423463658018292
32	21.474798907142496	29.354063109354662	27.360920473571582	21.81021750993126
33	20.887769792211806	25.143172154855005	29.253307160377023	24.715750892556166
34	20.05099256715356	23.683980176592957	33.83630603734861	22.428721218904872
35	19.91317481808988	24.247729266343974	29.40937644918157	26.429719466384576
36	23.377242564825558	24.053480736244765	30.499254107993245	22.070022590936432
37	21.50776342279692	26.210328509091312	30.779918091559406	21.50198997655236
38	19.05404876885915	26.22131668097612	27.798771187150916	26.925863363013814
39	22.168171177093946	24.933093207633615	31.804425439666556	21.094310175605884
40	19.93589612266525	22.498188814041022	34.77328049078018	22.792634572513553
41	20.540245576329617	24.546458549449568	30.580827316222823	24.33246855799799
42	20.669310035925733	23.291572072164353	31.81299248893268	24.226125402977235
43	24.364874353048098	23.243708340394942	28.36680380153499	24.024613505021968
44	20.488098319927143	23.86258452977143	30.45139037622383	25.1979267740776
45	19.880210302435465	23.29809047921466	29.248278674938216	27.573420543411657
46	23.909703300735092	24.361149549019352	28.760515587373657	22.9686315628719
47	18.496631845957005	22.853535118383583	35.28916584876178	23.36066718689763
48	20.04391543949894	24.084769090086247	30.000689088745318	25.870626381669492
49	20.565946724127976	21.644091250249094	34.128330673202456	23.66163135242047
50	20.475806466632275	21.313142412294834	32.288277483000925	25.922773638071966
51	18.8381963753932	21.31779841733077	29.642362941179755	30.201642266096272
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	127.0
1	251.5
2	376.0
3	1944.5
4	3513.0
5	2361.0
6	1209.0
7	1093.0
8	977.0
9	927.0
10	877.0
11	952.0
12	1027.0
13	1027.5
14	1028.0
15	1020.5
16	1013.0
17	1022.5
18	1032.0
19	1174.5
20	1317.0
21	1523.0
22	1729.0
23	2150.0
24	2571.0
25	2986.5
26	4567.0
27	5732.0
28	6716.0
29	7700.0
30	9022.5
31	10345.0
32	11317.0
33	12289.0
34	13910.5
35	15532.0
36	17167.5
37	18803.0
38	20317.0
39	21831.0
40	24235.0
41	26639.0
42	28754.5
43	30870.0
44	38127.5
45	45385.0
46	62460.5
47	79536.0
48	69413.0
49	59290.0
50	56515.0
51	53740.0
52	44474.5
53	35209.0
54	29419.5
55	23630.0
56	20873.0
57	18116.0
58	16894.0
59	15672.0
60	14174.5
61	12677.0
62	10798.5
63	8920.0
64	7308.5
65	5697.0
66	4696.5
67	3696.0
68	3039.5
69	2383.0
70	1814.0
71	1245.0
72	1050.0
73	855.0
74	609.5
75	309.0
76	254.0
77	228.0
78	202.0
79	138.5
80	75.0
81	50.5
82	26.0
83	20.5
84	15.0
85	9.0
86	3.0
87	2.5
88	2.0
89	2.0
90	2.0
91	2.0
92	2.0
93	3.0
94	4.0
95	2.0
96	0.0
97	0.5
98	1.0
99	1.0
100	1.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	536941.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.25183278478858
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.75633330200012	26.000459283812415
2	10.8000455750919	7.6144280136247104
3	4.184350934879196	4.425181184077056
4	2.209117620179821	3.1150177979403657
5	1.3746702817607184	2.4229823453423527
6	0.9608625817969892	2.0323300237600628
7	0.7724096184824122	1.906019829847304
8	0.5702466398068783	1.608179135404771
9	0.4601528016097333	1.4599106656018483
>10	4.6659219999442145	35.20882523627662
>50	0.1981067899592075	4.33855488558433
>100	0.03865498340667304	2.413420515659111
>500	0.002684373847685628	0.6572937147807476
>1k	0.00483187292583413	2.4544895441956567
>5k	0.001610624308611377	4.342907824092661
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGC	8063	1.5016547441897712	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTC	7600	1.4154255309242543	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCG	7284	1.3565736272700353	No Hit
GCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC	2564	0.47751987648549843	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1863	0.3469654952778797	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCT	1639	0.3052476901559017	No Hit
GAACTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCT	1231	0.2292616879694417	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTC	1219	0.22702680555219287	No Hit
CTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGCT	1148	0.21380375125013734	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGC	1135	0.21138262863145113	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCG	1100	0.20486422158114206	No Hit
GAATATGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTC	1070	0.19927701553802002	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCATTCGTCGTA	849	0.1581179310203542	No Hit
CCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC	791	0.1473159993369849	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTT	739	0.13763150886224	No Hit
TGAATGATACCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCC	562	0.10466699320781986	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	5.587206043122056E-4	0.0	0.0	0.2586876397965512	0.0
2	5.587206043122056E-4	0.0	0.0	1.2900858753568827	0.0
3	5.587206043122056E-4	0.0	0.0	1.698510637109105	0.0
4	5.587206043122056E-4	0.0	0.0	2.4151629322402277	0.0
5	5.587206043122056E-4	0.0	0.0	5.449388294058378	0.0
6	5.587206043122056E-4	0.0	0.0	6.652686235545432	0.0
7	5.587206043122056E-4	0.0	0.0	8.021551716110336	0.0
8	5.587206043122056E-4	0.0	0.0	9.769229766398915	0.0
9	5.587206043122056E-4	0.0	0.0	10.312119953588942	0.0
10	5.587206043122056E-4	0.0	0.0	13.10423305353847	0.0
11	5.587206043122056E-4	0.0	0.0	14.956950577437745	0.0
12	5.587206043122056E-4	0.0	0.0	18.097891574679526	0.0
13	5.587206043122056E-4	0.0	0.0	18.789028962213724	0.0
14	5.587206043122056E-4	0.0	0.0	19.077701274441697	0.0
15	5.587206043122056E-4	0.0	0.0	19.696391223616747	0.0
16	5.587206043122056E-4	0.0	0.0	20.62815095140807	0.0
17	5.587206043122056E-4	0.0	0.0	21.68972009960126	0.0
18	5.587206043122056E-4	0.0	0.0	22.852790157577836	0.0
19	5.587206043122056E-4	0.0	0.0	23.596819762320255	0.0
20	5.587206043122056E-4	0.0	0.0	24.299317802142134	0.0
21	5.587206043122056E-4	0.0	0.0	25.11262876181927	0.0
22	5.587206043122056E-4	0.0	0.0	26.0220396654381	0.0
23	5.587206043122056E-4	0.0	0.0	26.88600795990621	0.0
24	5.587206043122056E-4	0.0	0.0	27.52611553224656	0.0
25	5.587206043122056E-4	0.0	0.0	28.14182563819861	0.0
26	5.587206043122056E-4	0.0	0.0	28.710603213388435	0.0
27	5.587206043122056E-4	0.0	0.0	29.317932510275803	0.0
28	5.587206043122056E-4	0.0	0.0	29.885406404055566	0.0
29	5.587206043122056E-4	0.0	0.0	30.482120009461003	0.0
30	5.587206043122056E-4	0.0	0.0	31.15891690148452	0.0
31	5.587206043122056E-4	0.0	0.0	31.78766382153719	0.0
32	5.587206043122056E-4	0.0	0.0	32.3787902208995	0.0
33	5.587206043122056E-4	0.0	0.0	32.95464492374395	0.0
34	5.587206043122056E-4	0.0	0.0	33.519138974300716	0.0
35	7.449608057496075E-4	0.0	0.0	34.08568166707329	0.0
36	7.449608057496075E-4	0.0	0.0	34.64496099198981	0.0
37	7.449608057496075E-4	0.0	0.0	35.21969825362563	0.0
38	7.449608057496075E-4	0.0	0.0	35.79722911828301	0.0
39	7.449608057496075E-4	0.0	0.0	36.38202335079646	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TTGTGCG	50	2.1827873E-11	45.000004	1
GATCACG	25	3.8880185E-5	45.000004	1
AGCGATT	50	2.1827873E-11	45.000004	22
ACCGCGC	20	7.0299243E-4	45.0	12
GGCGTAC	20	7.0299243E-4	45.0	9
ATTAACG	20	7.0299243E-4	45.0	1
TAGTTCG	20	7.0299243E-4	45.0	29
CGCCTAT	20	7.0299243E-4	45.0	40
AGTGCGT	20	7.0299243E-4	45.0	1
ACGATAG	20	7.0299243E-4	45.0	1
TACGCGG	70	0.0	45.0	2
GTTAACG	20	7.0299243E-4	45.0	1
CGTACTA	20	7.0299243E-4	45.0	44
CCGACGG	20	7.0299243E-4	45.0	2
TTACGCG	20	7.0299243E-4	45.0	1
AACTCGT	20	7.0299243E-4	45.0	29
GCGAGCC	20	7.0299243E-4	45.0	22
GCGATTC	30	2.1633332E-6	44.999996	9
GCGATAC	30	2.1633332E-6	44.999996	9
GCCTACG	30	2.1633332E-6	44.999996	1
>>END_MODULE