##FastQC 0.11.5 >>Basic Statistics pass #Measure Value Filename SRR2936778.fastq File type Conventional base calls Encoding Sanger / Illumina 1.9 Total Sequences 536941 Sequences flagged as poor quality 0 Sequence length 51 %GC 46 >>END_MODULE >>Per base sequence quality pass #Base Mean Median Lower Quartile Upper Quartile 10th Percentile 90th Percentile 1 31.899478713676178 33.0 31.0 34.0 30.0 34.0 2 31.976872691785502 33.0 31.0 34.0 30.0 34.0 3 31.862096580443662 33.0 31.0 34.0 30.0 34.0 4 35.62612838282046 37.0 35.0 37.0 33.0 37.0 5 30.920136476819614 37.0 35.0 37.0 0.0 37.0 6 33.22046556325555 37.0 35.0 37.0 17.0 37.0 7 35.128321361192384 37.0 35.0 37.0 32.0 37.0 8 35.50956995275086 37.0 35.0 37.0 33.0 37.0 9 37.5532395551839 39.0 37.0 39.0 35.0 39.0 10 37.27621656755584 39.0 37.0 39.0 34.0 39.0 11 37.10892258181067 39.0 37.0 39.0 34.0 39.0 12 37.10435224726739 39.0 37.0 39.0 33.0 39.0 13 37.0080586135162 39.0 37.0 39.0 33.0 39.0 14 38.25930037005928 40.0 37.0 41.0 34.0 41.0 15 38.227877923272764 40.0 37.0 41.0 33.0 41.0 16 38.22590005233349 40.0 37.0 41.0 34.0 41.0 17 38.1662603526272 40.0 37.0 41.0 33.0 41.0 18 38.06644305426481 40.0 37.0 41.0 33.0 41.0 19 37.99254107993243 40.0 37.0 41.0 33.0 41.0 20 37.99768503429613 40.0 37.0 41.0 33.0 41.0 21 38.02137292551696 40.0 37.0 41.0 33.0 41.0 22 38.05058656351443 40.0 37.0 41.0 34.0 41.0 23 38.08165515391821 40.0 37.0 41.0 34.0 41.0 24 38.021683946653354 40.0 37.0 41.0 34.0 41.0 25 37.90779433867036 40.0 37.0 41.0 33.0 41.0 26 37.82569779547474 40.0 36.0 41.0 33.0 41.0 27 37.83228324899756 40.0 37.0 41.0 33.0 41.0 28 37.816320601332365 40.0 36.0 41.0 33.0 41.0 29 37.78258877604802 40.0 36.0 41.0 33.0 41.0 30 37.722014150530505 40.0 36.0 41.0 33.0 41.0 31 37.701645059699295 40.0 36.0 41.0 33.0 41.0 32 37.58102473083635 40.0 36.0 41.0 33.0 41.0 33 37.49905296857569 40.0 36.0 41.0 33.0 41.0 34 37.36630467779514 40.0 36.0 41.0 32.0 41.0 35 37.27232042254177 40.0 36.0 41.0 32.0 41.0 36 37.15208933569983 40.0 36.0 41.0 32.0 41.0 37 37.06900944424061 40.0 36.0 41.0 31.0 41.0 38 37.03107604001185 39.0 35.0 41.0 31.0 41.0 39 36.906149837691665 39.0 35.0 41.0 31.0 41.0 40 36.79375015132016 39.0 35.0 41.0 31.0 41.0 41 36.752386202580915 39.0 35.0 41.0 31.0 41.0 42 36.6856172279636 39.0 35.0 41.0 30.0 41.0 43 36.629417384777845 39.0 35.0 41.0 30.0 41.0 44 36.51398757032896 39.0 35.0 41.0 30.0 41.0 45 36.46989334023664 39.0 35.0 41.0 30.0 41.0 46 36.27379544493715 39.0 35.0 40.0 30.0 41.0 47 36.1265744281029 39.0 35.0 40.0 30.0 41.0 48 36.00802322787792 39.0 35.0 40.0 29.0 41.0 49 35.912422407676075 39.0 35.0 40.0 28.0 41.0 50 35.73616840583975 38.0 35.0 40.0 27.0 41.0 51 33.79021903710091 37.0 32.0 40.0 22.0 41.0 >>END_MODULE >>Per sequence quality scores pass #Quality Count 7 1.0 8 10.0 9 16.0 10 18.0 11 22.0 12 9.0 13 10.0 14 12.0 15 16.0 16 29.0 17 52.0 18 140.0 19 262.0 20 436.0 21 638.0 22 985.0 23 1520.0 24 2129.0 25 3174.0 26 4148.0 27 5113.0 28 5765.0 29 7230.0 30 9162.0 31 12108.0 32 15824.0 33 22421.0 34 33197.0 35 42218.0 36 45592.0 37 69200.0 38 108377.0 39 147070.0 40 37.0 >>END_MODULE >>Per base sequence content fail #Base G A T C 1 44.88966199265841 20.65534202081793 23.882325991123793 10.572669995399867 2 30.724790991933936 30.046317938097484 24.000402278835104 15.228488791133476 3 29.06017607148644 29.965862171076523 26.942811221344616 14.03115053609242 4 26.17717775323546 24.471217508068857 32.7004270487819 16.65117768991379 5 23.85513492171393 37.46091283772333 22.00297611841897 16.680976122143772 6 24.333958479609493 36.950428445583405 25.602626731801077 13.112986343006028 7 75.45354145055043 5.6631920453085165 14.240857002910934 4.642409501230117 8 75.38872986045023 8.616216679299962 10.828005311570545 5.167048148679278 9 69.26924932162007 7.374553256316801 11.362514689695887 11.993682732367244 10 36.56770483162954 26.556549043563443 20.885907390197435 15.989838734609577 11 27.355147027327025 24.68744238193768 27.305607133744676 20.65180345699062 12 24.30118020415651 21.365289668697304 32.66802125373179 21.665508873414396 13 21.450587680955636 23.2738792530278 36.620038328233456 18.65549473778311 14 20.19681864487905 28.69589023747488 29.886337605062753 21.220953512583318 15 18.05263520573024 25.74677664771362 38.184642260509065 18.01594588604707 16 19.683540649717568 27.83285314401396 29.82152601496254 22.662080191305936 17 20.511750825509694 25.755716177382617 30.830202945947505 22.902330051160185 18 19.98562225644903 27.72725494979895 31.356517755209605 20.93060503854241 19 19.956755025226236 28.956067798882934 28.870583546423163 22.21659362946767 20 20.988153260786564 28.343710016556756 31.97260034156453 18.69553638109215 21 20.79576713270173 29.863988780890267 30.597216453949315 18.743027632458688 22 19.285731579447276 25.015970097273254 29.592078086791656 26.106220236487808 23 19.024436576830603 29.375108252117087 30.82834054393313 20.77211462711918 24 21.86385468794523 24.57625698167955 29.30470945597375 24.25517887440147 25 19.065781901549705 31.25930037005928 27.647357903382307 22.02755982500871 26 18.34857088581427 27.132962467012206 30.229764536513322 24.288702110660203 27 22.56858761018436 27.944224784473526 27.406549322923745 22.080638282418366 28 17.243421530484724 27.01414121849514 31.677037141883375 24.06540010913676 29 21.148692314425606 24.895658927144694 30.66947765210703 23.286171106322666 30 22.704542957233663 26.15259404664572 30.063452036629723 21.079410959490893 31 21.626212190911108 25.597225765959386 27.353098385111213 25.423463658018292 32 21.474798907142496 29.354063109354662 27.360920473571582 21.81021750993126 33 20.887769792211806 25.143172154855005 29.253307160377023 24.715750892556166 34 20.05099256715356 23.683980176592957 33.83630603734861 22.428721218904872 35 19.91317481808988 24.247729266343974 29.40937644918157 26.429719466384576 36 23.377242564825558 24.053480736244765 30.499254107993245 22.070022590936432 37 21.50776342279692 26.210328509091312 30.779918091559406 21.50198997655236 38 19.05404876885915 26.22131668097612 27.798771187150916 26.925863363013814 39 22.168171177093946 24.933093207633615 31.804425439666556 21.094310175605884 40 19.93589612266525 22.498188814041022 34.77328049078018 22.792634572513553 41 20.540245576329617 24.546458549449568 30.580827316222823 24.33246855799799 42 20.669310035925733 23.291572072164353 31.81299248893268 24.226125402977235 43 24.364874353048098 23.243708340394942 28.36680380153499 24.024613505021968 44 20.488098319927143 23.86258452977143 30.45139037622383 25.1979267740776 45 19.880210302435465 23.29809047921466 29.248278674938216 27.573420543411657 46 23.909703300735092 24.361149549019352 28.760515587373657 22.9686315628719 47 18.496631845957005 22.853535118383583 35.28916584876178 23.36066718689763 48 20.04391543949894 24.084769090086247 30.000689088745318 25.870626381669492 49 20.565946724127976 21.644091250249094 34.128330673202456 23.66163135242047 50 20.475806466632275 21.313142412294834 32.288277483000925 25.922773638071966 51 18.8381963753932 21.31779841733077 29.642362941179755 30.201642266096272 >>END_MODULE >>Per sequence GC content fail #GC Content Count 0 127.0 1 251.5 2 376.0 3 1944.5 4 3513.0 5 2361.0 6 1209.0 7 1093.0 8 977.0 9 927.0 10 877.0 11 952.0 12 1027.0 13 1027.5 14 1028.0 15 1020.5 16 1013.0 17 1022.5 18 1032.0 19 1174.5 20 1317.0 21 1523.0 22 1729.0 23 2150.0 24 2571.0 25 2986.5 26 4567.0 27 5732.0 28 6716.0 29 7700.0 30 9022.5 31 10345.0 32 11317.0 33 12289.0 34 13910.5 35 15532.0 36 17167.5 37 18803.0 38 20317.0 39 21831.0 40 24235.0 41 26639.0 42 28754.5 43 30870.0 44 38127.5 45 45385.0 46 62460.5 47 79536.0 48 69413.0 49 59290.0 50 56515.0 51 53740.0 52 44474.5 53 35209.0 54 29419.5 55 23630.0 56 20873.0 57 18116.0 58 16894.0 59 15672.0 60 14174.5 61 12677.0 62 10798.5 63 8920.0 64 7308.5 65 5697.0 66 4696.5 67 3696.0 68 3039.5 69 2383.0 70 1814.0 71 1245.0 72 1050.0 73 855.0 74 609.5 75 309.0 76 254.0 77 228.0 78 202.0 79 138.5 80 75.0 81 50.5 82 26.0 83 20.5 84 15.0 85 9.0 86 3.0 87 2.5 88 2.0 89 2.0 90 2.0 91 2.0 92 2.0 93 3.0 94 4.0 95 2.0 96 0.0 97 0.5 98 1.0 99 1.0 100 1.0 >>END_MODULE >>Per base N content pass #Base N-Count 1 0.0 2 0.0 3 0.0 4 0.0 5 0.0 6 0.0 7 0.0 8 0.0 9 0.0 10 0.0 11 0.0 12 0.0 13 0.0 14 0.0 15 0.0 16 0.0 17 0.0 18 0.0 19 0.0 20 0.0 21 0.0 22 0.0 23 0.0 24 0.0 25 0.0 26 0.0 27 0.0 28 0.0 29 0.0 30 0.0 31 0.0 32 0.0 33 0.0 34 0.0 35 0.0 36 0.0 37 0.0 38 0.0 39 0.0 40 0.0 41 0.0 42 0.0 43 0.0 44 0.0 45 0.0 46 0.0 47 0.0 48 0.0 49 0.0 50 0.0 51 0.0 >>END_MODULE >>Sequence Length Distribution pass #Length Count 51 536941.0 >>END_MODULE >>Sequence Duplication Levels fail #Total Deduplicated Percentage 35.25183278478858 #Duplication Level Percentage of deduplicated Percentage of total 1 73.75633330200012 26.000459283812415 2 10.8000455750919 7.6144280136247104 3 4.184350934879196 4.425181184077056 4 2.209117620179821 3.1150177979403657 5 1.3746702817607184 2.4229823453423527 6 0.9608625817969892 2.0323300237600628 7 0.7724096184824122 1.906019829847304 8 0.5702466398068783 1.608179135404771 9 0.4601528016097333 1.4599106656018483 >10 4.6659219999442145 35.20882523627662 >50 0.1981067899592075 4.33855488558433 >100 0.03865498340667304 2.413420515659111 >500 0.002684373847685628 0.6572937147807476 >1k 0.00483187292583413 2.4544895441956567 >5k 0.001610624308611377 4.342907824092661 >10k+ 0.0 0.0 >>END_MODULE >>Overrepresented sequences fail #Sequence Count Percentage Possible Source GAATGATACGGCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGC 8063 1.5016547441897712 No Hit GAATCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTC 7600 1.4154255309242543 No Hit GAATGATACCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCG 7284 1.3565736272700353 No Hit GCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC 2564 0.47751987648549843 No Hit CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT 1863 0.3469654952778797 No Hit GAATGACTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCT 1639 0.3052476901559017 No Hit GAACTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCT 1231 0.2292616879694417 No Hit GAATGATCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTC 1219 0.22702680555219287 No Hit CTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGCT 1148 0.21380375125013734 No Hit GAATAATACGGCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGC 1135 0.21138262863145113 No Hit GAATAATACCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCG 1100 0.20486422158114206 No Hit GAATATGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTC 1070 0.19927701553802002 No Hit GAATGATACGGCGACTGTCTCTTATACACATCTGACGCTGCATTCGTCGTA 849 0.1581179310203542 No Hit CCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTCTGC 791 0.1473159993369849 No Hit GAATCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCCGTCTTT 739 0.13763150886224 No Hit TGAATGATACCTGTCTCTTATACACATCTGACGCTGCATTCGTCGTATGCC 562 0.10466699320781986 No Hit >>END_MODULE >>Adapter Content fail #Position Illumina Universal Adapter Illumina Small RNA 3' Adapter Illumina Small RNA 5' Adapter Nextera Transposase Sequence SOLID Small RNA Adapter 1 5.587206043122056E-4 0.0 0.0 0.2586876397965512 0.0 2 5.587206043122056E-4 0.0 0.0 1.2900858753568827 0.0 3 5.587206043122056E-4 0.0 0.0 1.698510637109105 0.0 4 5.587206043122056E-4 0.0 0.0 2.4151629322402277 0.0 5 5.587206043122056E-4 0.0 0.0 5.449388294058378 0.0 6 5.587206043122056E-4 0.0 0.0 6.652686235545432 0.0 7 5.587206043122056E-4 0.0 0.0 8.021551716110336 0.0 8 5.587206043122056E-4 0.0 0.0 9.769229766398915 0.0 9 5.587206043122056E-4 0.0 0.0 10.312119953588942 0.0 10 5.587206043122056E-4 0.0 0.0 13.10423305353847 0.0 11 5.587206043122056E-4 0.0 0.0 14.956950577437745 0.0 12 5.587206043122056E-4 0.0 0.0 18.097891574679526 0.0 13 5.587206043122056E-4 0.0 0.0 18.789028962213724 0.0 14 5.587206043122056E-4 0.0 0.0 19.077701274441697 0.0 15 5.587206043122056E-4 0.0 0.0 19.696391223616747 0.0 16 5.587206043122056E-4 0.0 0.0 20.62815095140807 0.0 17 5.587206043122056E-4 0.0 0.0 21.68972009960126 0.0 18 5.587206043122056E-4 0.0 0.0 22.852790157577836 0.0 19 5.587206043122056E-4 0.0 0.0 23.596819762320255 0.0 20 5.587206043122056E-4 0.0 0.0 24.299317802142134 0.0 21 5.587206043122056E-4 0.0 0.0 25.11262876181927 0.0 22 5.587206043122056E-4 0.0 0.0 26.0220396654381 0.0 23 5.587206043122056E-4 0.0 0.0 26.88600795990621 0.0 24 5.587206043122056E-4 0.0 0.0 27.52611553224656 0.0 25 5.587206043122056E-4 0.0 0.0 28.14182563819861 0.0 26 5.587206043122056E-4 0.0 0.0 28.710603213388435 0.0 27 5.587206043122056E-4 0.0 0.0 29.317932510275803 0.0 28 5.587206043122056E-4 0.0 0.0 29.885406404055566 0.0 29 5.587206043122056E-4 0.0 0.0 30.482120009461003 0.0 30 5.587206043122056E-4 0.0 0.0 31.15891690148452 0.0 31 5.587206043122056E-4 0.0 0.0 31.78766382153719 0.0 32 5.587206043122056E-4 0.0 0.0 32.3787902208995 0.0 33 5.587206043122056E-4 0.0 0.0 32.95464492374395 0.0 34 5.587206043122056E-4 0.0 0.0 33.519138974300716 0.0 35 7.449608057496075E-4 0.0 0.0 34.08568166707329 0.0 36 7.449608057496075E-4 0.0 0.0 34.64496099198981 0.0 37 7.449608057496075E-4 0.0 0.0 35.21969825362563 0.0 38 7.449608057496075E-4 0.0 0.0 35.79722911828301 0.0 39 7.449608057496075E-4 0.0 0.0 36.38202335079646 0.0 >>END_MODULE >>Kmer Content fail #Sequence Count PValue Obs/Exp Max Max Obs/Exp Position TTGTGCG 50 2.1827873E-11 45.000004 1 GATCACG 25 3.8880185E-5 45.000004 1 AGCGATT 50 2.1827873E-11 45.000004 22 ACCGCGC 20 7.0299243E-4 45.0 12 GGCGTAC 20 7.0299243E-4 45.0 9 ATTAACG 20 7.0299243E-4 45.0 1 TAGTTCG 20 7.0299243E-4 45.0 29 CGCCTAT 20 7.0299243E-4 45.0 40 AGTGCGT 20 7.0299243E-4 45.0 1 ACGATAG 20 7.0299243E-4 45.0 1 TACGCGG 70 0.0 45.0 2 GTTAACG 20 7.0299243E-4 45.0 1 CGTACTA 20 7.0299243E-4 45.0 44 CCGACGG 20 7.0299243E-4 45.0 2 TTACGCG 20 7.0299243E-4 45.0 1 AACTCGT 20 7.0299243E-4 45.0 29 GCGAGCC 20 7.0299243E-4 45.0 22 GCGATTC 30 2.1633332E-6 44.999996 9 GCGATAC 30 2.1633332E-6 44.999996 9 GCCTACG 30 2.1633332E-6 44.999996 1 >>END_MODULE