SRR2936777.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936777.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	499836
Sequences flagged as poor quality	0
Sequence length	51
%GC	45

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTC	7437	1.4878880272729456	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGC	6989	1.3982586288302563	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCG	6486	1.2976256212037547	No Hit
GCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC	2690	0.538176521899183	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2229	0.44594627037668355	No Hit
CCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC	1646	0.3293080130282733	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCT	1625	0.32510663497627224	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTG	1570	0.3141030257924599	No Hit
CTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGCT	1296	0.2592850454949224	TruSeq Adapter, Index 16 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCT	1254	0.2508822893909202	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTC	1135	0.22707448042958092	No Hit
GAATATGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTC	1086	0.21727126497491175	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGC	941	0.18826174985395208	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCG	908	0.18165958434366472	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTT	864	0.17285669699661488	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	769	0.1538504629518482	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTA	710	0.14204659128194047	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTT	667	0.13344376955641452	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTC	580	0.1160380604838387	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCT	578	0.11563792924079098	No Hit
CGTCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCT	546	0.10923582935202746	No Hit
TCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC	545	0.10903576373050361	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCC	508	0.1016333357341208	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGCG	30	2.1630294E-6	45.000004	20
GTCCGTT	30	2.1630294E-6	45.000004	28
CGGGACG	30	2.1630294E-6	45.000004	6
ATTGCGG	30	2.1630294E-6	45.000004	2
AACGTTG	20	7.0294575E-4	45.0	23
TTTAGCG	50	2.1827873E-11	45.0	1
TTAGGCG	20	7.0294575E-4	45.0	1
AATTTCG	20	7.0294575E-4	45.0	15
GATCGAT	25	3.887632E-5	45.0	9
CCGATTC	20	7.0294575E-4	45.0	24
ACGTTGC	20	7.0294575E-4	45.0	24
CCGATCA	20	7.0294575E-4	45.0	12
ATGTCGC	25	3.887632E-5	45.0	11
ACGGGCG	40	6.8012014E-9	45.0	5
ATGTACG	40	6.8012014E-9	45.0	1
GTCGTCA	25	3.887632E-5	45.0	31
AACGATC	20	7.0294575E-4	45.0	21
CATACGG	25	3.887632E-5	45.0	2
CTCGCGG	25	3.887632E-5	45.0	2
AGTTCGC	20	7.0294575E-4	45.0	32

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content