##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936777.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	499836
Sequences flagged as poor quality	0
Sequence length	51
%GC	45
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.834827823526116	31.0	31.0	34.0	30.0	34.0
2	31.909418289198857	33.0	31.0	34.0	30.0	34.0
3	31.779439656207234	33.0	31.0	34.0	30.0	34.0
4	35.546721324594465	37.0	35.0	37.0	33.0	37.0
5	31.025602397586407	37.0	35.0	37.0	0.0	37.0
6	33.25912699365392	37.0	35.0	37.0	17.0	37.0
7	35.136400739442536	37.0	35.0	37.0	32.0	37.0
8	35.51007530469994	37.0	35.0	37.0	33.0	37.0
9	37.51079554093743	39.0	37.0	39.0	35.0	39.0
10	37.255261725846076	39.0	37.0	39.0	34.0	39.0
11	37.07141542425916	39.0	37.0	39.0	33.0	39.0
12	37.06907865779976	39.0	37.0	39.0	33.0	39.0
13	36.98034755399771	39.0	37.0	39.0	33.0	39.0
14	38.19057050712634	40.0	37.0	41.0	33.0	41.0
15	38.153208252306754	40.0	37.0	41.0	33.0	41.0
16	38.16160100512968	40.0	37.0	41.0	33.0	41.0
17	38.12003937291431	40.0	37.0	41.0	33.0	41.0
18	37.98120983682648	40.0	37.0	41.0	33.0	41.0
19	37.928228458934534	40.0	37.0	41.0	33.0	41.0
20	37.93019910530654	40.0	36.0	41.0	33.0	41.0
21	37.92655190902616	40.0	36.0	41.0	33.0	41.0
22	37.950173656959485	40.0	36.0	41.0	33.0	41.0
23	37.98698173000744	40.0	37.0	41.0	34.0	41.0
24	37.9480749685897	40.0	36.0	41.0	33.0	41.0
25	37.82050712633744	40.0	36.0	41.0	33.0	41.0
26	37.75618202770509	40.0	36.0	41.0	33.0	41.0
27	37.71917588969182	40.0	36.0	41.0	33.0	41.0
28	37.63251346441633	40.0	36.0	41.0	33.0	41.0
29	37.59539729031122	40.0	36.0	41.0	33.0	41.0
30	37.51822997943325	40.0	36.0	41.0	33.0	41.0
31	37.32456845845437	39.0	36.0	41.0	32.0	41.0
32	37.229437255419775	39.0	35.0	41.0	32.0	41.0
33	37.146179947022624	39.0	35.0	41.0	31.0	41.0
34	37.01656743411839	39.0	35.0	41.0	31.0	41.0
35	36.99669691658864	39.0	35.0	41.0	31.0	41.0
36	36.775380324746514	39.0	35.0	41.0	30.0	41.0
37	36.71442433118063	39.0	35.0	41.0	30.0	41.0
38	36.567458126265414	39.0	35.0	41.0	30.0	41.0
39	36.581122608215495	39.0	35.0	41.0	30.0	41.0
40	36.60874366792308	39.0	35.0	41.0	30.0	41.0
41	36.56597163869749	39.0	35.0	41.0	30.0	41.0
42	36.50606198833217	39.0	35.0	41.0	30.0	41.0
43	36.37931841644059	39.0	35.0	40.0	30.0	41.0
44	36.2233352539633	39.0	35.0	40.0	30.0	41.0
45	36.1653322289711	39.0	35.0	40.0	29.0	41.0
46	35.9794532606695	39.0	35.0	40.0	28.0	41.0
47	35.8398934850631	38.0	35.0	40.0	28.0	41.0
48	35.6923510911579	38.0	35.0	40.0	27.0	41.0
49	35.580958554405846	38.0	35.0	40.0	27.0	41.0
50	35.37389063612865	38.0	34.0	40.0	26.0	41.0
51	33.44070455109276	36.0	31.0	40.0	21.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	11.0
9	22.0
10	19.0
11	17.0
12	9.0
13	13.0
14	12.0
15	19.0
16	35.0
17	62.0
18	143.0
19	250.0
20	395.0
21	694.0
22	1012.0
23	1563.0
24	2324.0
25	3327.0
26	4524.0
27	5299.0
28	6109.0
29	7386.0
30	9219.0
31	11876.0
32	15448.0
33	21583.0
34	31824.0
35	41363.0
36	43437.0
37	64414.0
38	100483.0
39	126917.0
40	24.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.76615529893805	20.362879024319977	24.41160700709833	11.459358669643644
2	30.693067326082957	29.531086196272376	24.282964812458484	15.49288166518618
3	29.209980873726582	28.742427516225323	27.63886554789971	14.408726062148386
4	25.66301746973007	24.14111828679807	33.48878432125737	16.707079922214486
5	23.81221040501284	36.549588264950906	22.89250874286766	16.74569258716859
6	23.89603790043134	35.74512440080346	26.80419177490217	13.554645923863026
7	74.26615930025048	5.194303731623973	14.8884834225626	5.651053545562944
8	73.43008506790227	8.160676701958243	12.264022599412607	6.145215630726878
9	67.37349850751046	7.240374842948487	13.134308053041396	12.251818596499652
10	35.477836730447585	28.465936827279346	21.353203850863085	14.703022591409983
11	24.35358797685641	23.6927712289631	30.840315623524518	21.113325170655976
12	22.23509311054026	21.447234692979297	34.26243807969014	22.055234116790306
13	21.68431245448507	23.601941436791268	37.3704575100633	17.34328859866036
14	19.23470898454693	28.658600020806823	30.81990893012908	21.286782064517162
15	16.229923414880083	26.585319984955063	39.18505269728471	17.999703902880146
16	18.536680031050185	28.617786634015957	30.069262718171558	22.776270616762297
17	19.54941220720396	26.424867356492932	30.609439896285984	23.416280540017127
18	19.24811338118903	27.341568034315255	32.8349698701174	20.575348714378315
19	19.508198689170047	28.437327443401433	29.88120103393913	22.173272833489385
20	20.241439192054994	28.827055274129915	32.043910402612056	18.88759513120303
21	19.796893381028976	30.196704519082257	31.960282972815087	18.04611912707368
22	18.45345273249626	24.75692026984851	30.91894141278339	25.87068558487184
23	17.670195824230348	28.188045678982704	31.658784081178627	22.48297441560832
24	20.726198193007306	24.193935610880366	30.296137132979617	24.783729063132707
25	18.119543210172935	31.632575484759002	28.12222408950136	22.125657215566704
26	19.394161284901447	27.007658511991934	29.406045182819966	24.192135020286653
27	21.874774926175785	28.201650141246326	27.980377563840943	21.943197368736946
28	17.42531550348514	25.21827159308253	33.620827631463115	23.735585271969207
29	23.65175777655071	24.60447026624733	28.95109595947471	22.792675997727255
30	23.316647860498243	25.977920758008626	30.645251642538753	20.060179738954375
31	22.215886810873968	25.87948847221889	29.1765699149321	22.72805480197505
32	22.433358141470404	29.6059107387223	28.01658944133676	19.94414167847054
33	20.965076545106793	24.240350835073905	31.102601653342298	23.691970966477005
34	21.739930697268704	23.3112460887171	31.020174617274463	23.92864859673973
35	21.06430909338263	23.819612833009227	32.01950239678615	23.096575676822
36	26.26221400619403	22.920317864259477	31.224241551228804	19.593226578317687
37	21.458638433406158	25.803063404796774	33.08785281572356	19.65044534607351
38	18.40703750830272	26.70515929224786	33.002224729711344	21.885578469738075
39	21.971406621371813	25.576589121231763	29.824982594290926	22.6270216631055
40	20.70659176209797	22.31671988412199	35.5374562856617	21.439232068118343
41	21.612889027601053	24.793532278587378	28.575172656631377	25.018406037180196
42	22.714650405332947	22.61921910386607	33.405757088324975	21.260373402476013
43	25.291495610560265	22.677038068486464	29.694539809057368	22.336926511895904
44	21.308789282884785	23.21861570595155	30.579830184300448	24.89276482686321
45	20.4335021887179	22.570403092214246	29.881000968317608	27.115093750750248
46	25.241279139557776	24.286165862402868	28.539761041621652	21.932793956417704
47	18.74954985235157	22.958530397970534	35.71591482006098	22.576004929616914
48	21.37841211917509	23.026552709288648	30.85231956081595	24.742715610720317
49	21.1747453164638	20.232036107843374	36.051224801734975	22.54199377395786
50	20.896654102545632	20.4419049448219	33.45277250938308	25.208668443249387
51	20.307860978400917	19.726670347874105	29.746957001896625	30.218511671828356
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	962.0
1	885.0
2	808.0
3	2341.5
4	3875.0
5	2639.5
6	1404.0
7	1213.5
8	1023.0
9	1068.5
10	1114.0
11	1063.0
12	1012.0
13	1000.5
14	989.0
15	974.0
16	959.0
17	1045.5
18	1132.0
19	1264.5
20	1397.0
21	1316.5
22	1236.0
23	1833.5
24	2431.0
25	2993.5
26	4459.5
27	5363.0
28	5918.0
29	6473.0
30	7703.5
31	8934.0
32	9379.0
33	9824.0
34	12195.0
35	14566.0
36	15592.5
37	16619.0
38	18290.0
39	19961.0
40	22041.5
41	24122.0
42	27144.0
43	30166.0
44	37297.0
45	44428.0
46	60883.0
47	77338.0
48	68018.5
49	58699.0
50	57061.5
51	55424.0
52	44447.5
53	33471.0
54	27802.0
55	22133.0
56	18165.5
57	14198.0
58	12580.5
59	10963.0
60	9943.0
61	8923.0
62	7646.0
63	6369.0
64	5282.0
65	4195.0
66	3459.5
67	2724.0
68	1995.5
69	1267.0
70	1063.5
71	860.0
72	645.0
73	430.0
74	339.0
75	159.0
76	70.0
77	81.5
78	93.0
79	71.0
80	49.0
81	29.5
82	10.0
83	8.5
84	7.0
85	6.5
86	6.0
87	4.0
88	2.0
89	1.0
90	0.0
91	0.5
92	1.0
93	1.0
94	1.0
95	1.0
96	1.0
97	0.5
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	499836.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	36.772496752058224
#Duplication Level	Percentage of deduplicated	Percentage of total
1	74.90088118503171	27.54292410102878
2	10.918340121504984	8.029892533118183
3	4.137500531914225	4.564386746143651
4	2.1375602653305537	3.14413711656786
5	1.3339044678161787	2.4525498855163197
6	0.8678581672123761	1.9147986981038583
7	0.6138579492514973	1.5801162611553061
8	0.4869568628424078	1.4325295725811922
9	0.3881659305316733	1.2846447377762056
>10	3.9761586756203284	32.71928679773749
>50	0.18885666660401837	4.371344877684309
>100	0.037332131770561536	2.270563701995805
>500	0.006039021315826131	1.53753117759403
>1k	0.00494101744022138	2.933543269645332
>5k	0.0016470058134071266	4.221750523351674
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTC	7437	1.4878880272729456	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGC	6989	1.3982586288302563	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCG	6486	1.2976256212037547	No Hit
GCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC	2690	0.538176521899183	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	2229	0.44594627037668355	No Hit
CCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC	1646	0.3293080130282733	TruSeq Adapter, Index 13 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCT	1625	0.32510663497627224	No Hit
CGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTG	1570	0.3141030257924599	No Hit
CTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGCT	1296	0.2592850454949224	TruSeq Adapter, Index 16 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCT	1254	0.2508822893909202	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTC	1135	0.22707448042958092	No Hit
GAATATGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTC	1086	0.21727126497491175	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGC	941	0.18826174985395208	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCG	908	0.18165958434366472	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTT	864	0.17285669699661488	No Hit
AAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAAA	769	0.1538504629518482	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGGTATCTGTCGTA	710	0.14204659128194047	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTT	667	0.13344376955641452	No Hit
CGTTCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTC	580	0.1160380604838387	No Hit
CGTTTTCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCT	578	0.11563792924079098	No Hit
CGTCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCT	546	0.10923582935202746	No Hit
TCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCCGTCTTCTGC	545	0.10903576373050361	TruSeq Adapter, Index 13 (95% over 21bp)
CGTTTTTTTTCTGTCTCTTATACACATCTGACGCGGTATCTGTCGTATGCC	508	0.1016333357341208	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3073007946606487	0.0
2	0.0	0.0	0.0	1.6533422962731776	0.0
3	0.0	0.0	0.0	2.396185948991269	0.0
4	0.0	0.0	0.0	3.3304924015076947	0.0
5	0.0	0.0	0.0	6.745812626541506	0.0
6	0.0	0.0	0.0	8.300722637024943	0.0
7	0.0	0.0	0.0	9.96326795188822	0.0
8	0.0	0.0	0.0	11.92671196152338	0.0
9	0.0	0.0	0.0	12.561920309861634	0.0
10	0.0	0.0	0.0	15.485479237189798	0.0
11	0.0	0.0	0.0	17.549356188829936	0.0
12	0.0	0.0	0.0	20.748005345753405	0.0
13	0.0	0.0	0.0	21.488248145391687	0.0
14	0.0	0.0	0.0	21.801550908698054	0.0
15	0.0	0.0	0.0	22.42295472915116	0.0
16	0.0	0.0	0.0	23.43568690530494	0.0
17	0.0	0.0	0.0	24.74291567634184	0.0
18	0.0	0.0	0.0	26.07035107515265	0.0
19	0.0	0.0	0.0	26.959042565961635	0.0
20	0.0	0.0	0.0	27.751102361574596	0.0
21	0.0	0.0	0.0	28.648596739730632	0.0
22	0.0	0.0	0.0	29.707143943213374	0.0
23	0.0	0.0	0.0	30.72587808801287	0.0
24	0.0	0.0	0.0	31.477524628078008	0.0
25	0.0	0.0	0.0	32.1515457069919	0.0
26	0.0	0.0	0.0	32.78895477716691	0.0
27	0.0	0.0	0.0	33.45277250938308	0.0
28	2.0006562152385983E-4	0.0	0.0	34.11759056970686	0.0
29	2.0006562152385983E-4	0.0	0.0	34.76420265847198	0.0
30	2.0006562152385983E-4	0.0	0.0	35.506846245568546	0.0
31	2.0006562152385983E-4	0.0	0.0	36.16126089357309	0.0
32	2.0006562152385983E-4	0.0	0.0	36.802671276178586	0.0
33	2.0006562152385983E-4	0.0	0.0	37.4358789683016	0.0
34	2.0006562152385983E-4	0.0	0.0	38.02467209244632	0.0
35	2.0006562152385983E-4	0.0	0.0	38.656679390840196	0.0
36	2.0006562152385983E-4	0.0	0.0	39.20866044062453	0.0
37	2.0006562152385983E-4	0.0	0.0	39.79325218671724	0.0
38	2.0006562152385983E-4	0.0	0.0	40.39284885442425	0.0
39	2.0006562152385983E-4	0.0	0.0	41.00264886882898	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
ACCGGCG	30	2.1630294E-6	45.000004	20
GTCCGTT	30	2.1630294E-6	45.000004	28
CGGGACG	30	2.1630294E-6	45.000004	6
ATTGCGG	30	2.1630294E-6	45.000004	2
AACGTTG	20	7.0294575E-4	45.0	23
TTTAGCG	50	2.1827873E-11	45.0	1
TTAGGCG	20	7.0294575E-4	45.0	1
AATTTCG	20	7.0294575E-4	45.0	15
GATCGAT	25	3.887632E-5	45.0	9
CCGATTC	20	7.0294575E-4	45.0	24
ACGTTGC	20	7.0294575E-4	45.0	24
CCGATCA	20	7.0294575E-4	45.0	12
ATGTCGC	25	3.887632E-5	45.0	11
ACGGGCG	40	6.8012014E-9	45.0	5
ATGTACG	40	6.8012014E-9	45.0	1
GTCGTCA	25	3.887632E-5	45.0	31
AACGATC	20	7.0294575E-4	45.0	21
CATACGG	25	3.887632E-5	45.0	2
CTCGCGG	25	3.887632E-5	45.0	2
AGTTCGC	20	7.0294575E-4	45.0	32
>>END_MODULE