##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936776.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	941153
Sequences flagged as poor quality	0
Sequence length	51
%GC	44
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.08033869094611	33.0	31.0	34.0	30.0	34.0
2	32.174390348859326	33.0	31.0	34.0	30.0	34.0
3	31.884290864503434	33.0	31.0	34.0	30.0	34.0
4	35.70167124792675	37.0	35.0	37.0	33.0	37.0
5	30.82883548158482	37.0	35.0	37.0	0.0	37.0
6	33.26250035860269	37.0	35.0	37.0	17.0	37.0
7	35.28907308376002	37.0	35.0	37.0	32.0	37.0
8	35.77172255733127	37.0	35.0	37.0	35.0	37.0
9	37.7876572672031	39.0	37.0	39.0	35.0	39.0
10	37.48048723215035	39.0	37.0	39.0	35.0	39.0
11	37.344677220388185	39.0	37.0	39.0	34.0	39.0
12	37.38193364947038	39.0	37.0	39.0	35.0	39.0
13	37.36682027258055	39.0	37.0	39.0	34.0	39.0
14	38.654835079949805	40.0	38.0	41.0	35.0	41.0
15	38.7160238558449	40.0	38.0	41.0	35.0	41.0
16	38.65325191546964	40.0	38.0	41.0	35.0	41.0
17	38.619992711068235	40.0	38.0	41.0	34.0	41.0
18	38.479301452579975	40.0	38.0	41.0	34.0	41.0
19	38.387648979496426	40.0	37.0	41.0	34.0	41.0
20	38.29084325290362	40.0	37.0	41.0	34.0	41.0
21	38.207849308242125	40.0	37.0	41.0	34.0	41.0
22	38.18874401930398	40.0	37.0	41.0	34.0	41.0
23	38.16409021699979	40.0	37.0	41.0	34.0	41.0
24	38.16035649889019	40.0	37.0	41.0	34.0	41.0
25	37.987774570128344	40.0	36.0	41.0	34.0	41.0
26	37.97203111502593	40.0	36.0	41.0	34.0	41.0
27	37.91393429123639	40.0	36.0	41.0	34.0	41.0
28	37.78711219111027	40.0	36.0	41.0	33.0	41.0
29	37.72048859218427	40.0	36.0	41.0	33.0	41.0
30	37.62665581472938	40.0	36.0	41.0	33.0	41.0
31	37.52982458750065	40.0	36.0	41.0	33.0	41.0
32	37.38957852761453	40.0	36.0	41.0	33.0	41.0
33	37.16297562670469	40.0	36.0	41.0	32.0	41.0
34	36.94847809017237	40.0	36.0	41.0	31.0	41.0
35	36.78559809085239	40.0	35.0	41.0	30.0	41.0
36	36.6119823238092	40.0	35.0	41.0	30.0	41.0
37	36.58930588331547	40.0	35.0	41.0	30.0	41.0
38	36.49580036402158	40.0	35.0	41.0	30.0	41.0
39	36.40163395324671	39.0	35.0	41.0	30.0	41.0
40	36.36086056146025	39.0	35.0	41.0	30.0	41.0
41	36.29643745490903	39.0	35.0	41.0	29.0	41.0
42	36.24070262752177	39.0	35.0	41.0	29.0	41.0
43	36.153792210193245	39.0	35.0	41.0	29.0	41.0
44	36.02283156936226	39.0	35.0	41.0	28.0	41.0
45	35.976723232035596	39.0	35.0	41.0	28.0	41.0
46	35.82648836055349	39.0	35.0	41.0	27.0	41.0
47	35.79086928480279	39.0	35.0	41.0	27.0	41.0
48	35.69846241790655	39.0	35.0	41.0	26.0	41.0
49	35.54056673038284	39.0	35.0	40.0	26.0	41.0
50	35.36535398601502	39.0	35.0	40.0	25.0	41.0
51	33.79009682803965	37.0	32.0	40.0	20.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	4.0
8	25.0
9	41.0
10	54.0
11	38.0
12	22.0
13	13.0
14	21.0
15	25.0
16	39.0
17	99.0
18	239.0
19	548.0
20	998.0
21	1601.0
22	2502.0
23	3851.0
24	5640.0
25	8796.0
26	11840.0
27	13008.0
28	13460.0
29	13668.0
30	15344.0
31	18208.0
32	23100.0
33	32028.0
34	49402.0
35	65132.0
36	71605.0
37	114163.0
38	189135.0
39	286416.0
40	88.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	38.685845978284085	21.421065437819355	24.62819541562318	15.264893168273383
2	34.52212339545218	24.326331637895223	25.378232869682186	15.773312096970418
3	30.917821013161518	23.31810024512486	30.268936081593534	15.495142660120088
4	27.289080521445502	24.277455419044514	30.83494394641466	17.59852011309532
5	21.116226585900485	39.26545418226367	24.637120638195913	14.98119859363993
6	24.954816060725516	33.526217310044174	27.77295508806751	13.746011541162808
7	78.20237517173084	5.187041851856181	12.37641488684624	4.234168089566734
8	80.9033175264808	4.098908466529884	11.143459140012304	3.8543148669769955
9	74.06054063473208	7.707673460106912	13.073857279315904	5.1579286258451065
10	33.230303680698036	35.57668094348102	19.64738995678705	11.545625419033888
11	23.074144161470027	25.602213455198036	33.49072892505257	17.832913458279364
12	21.659177625742043	23.12355164356911	35.37979478363242	19.83747594705643
13	22.00938635907233	23.368782759020053	35.89873272464732	18.723098157260296
14	18.632145889138112	27.80089953493215	33.74754157931814	19.8194129966116
15	18.166015515011903	27.19292187348922	36.48365356111068	18.157409050388193
16	21.50649256815842	27.93477787352322	32.88222000036126	17.6765095579571
17	22.146877287752364	26.118070069372358	31.500829301930715	20.234223340944567
18	22.632876907367876	25.784224244092087	32.823674790390086	18.75922405814995
19	21.63558953751409	27.73279158649019	31.24040405757619	19.391214818419535
20	22.058793841171415	27.616338682445896	31.326362451163625	18.998505025219067
21	23.05767500077033	27.33360038165952	32.24438534435953	17.364339273210625
22	21.3957773071966	24.7332792861522	32.400470486732765	21.47047291991844
23	19.50788022776318	26.477522783224405	32.96244075086622	21.05215623814619
24	19.850438770316835	25.762548703558295	33.357594355009226	21.02941817111564
25	20.461497758600355	27.73109154409538	31.081131335712687	20.72627936159158
26	19.625501910953904	28.046980671580496	31.009835807780455	21.317681609685142
27	20.111395277919744	27.163808647478145	32.18934647182764	20.53544960277447
28	18.477654536510006	28.021692540957737	32.77054846555236	20.730104456979895
29	19.449866281040386	27.103775900411513	31.818949735059014	21.627408083489083
30	20.507080145311125	26.48241040510948	32.19274655661726	20.817762892962143
31	20.55648762741021	27.725885164261282	30.708397040651203	21.009230167677305
32	19.677353203995523	28.644651826004914	30.556774509564335	21.121220460435232
33	19.24129232972747	27.11323238623263	31.144564167568927	22.500911116470967
34	20.1834345743997	27.17167134355413	30.794886697487016	21.850007384559152
35	19.144177407924108	27.17145883825478	30.69511545944177	22.98924829437934
36	19.179878298215062	28.831443984134353	30.184252719802203	21.804424997848386
37	20.123295574683393	28.792449261703464	29.766254795978973	21.31800036763417
38	19.084675924105856	28.650814479686087	29.072318740948607	23.192190855259454
39	21.00009243980522	26.996566976888985	28.78564909212423	23.21769149118156
40	21.664915268824515	25.48087292926867	30.664195938386214	22.190015863520596
41	19.731010792081626	27.56310610495849	29.183884023107826	23.521999079852055
42	20.69312853489284	28.052399556713947	29.559805897659576	21.69466601073364
43	20.172278046183777	27.24434815593214	30.16140840012198	22.421965397762104
44	20.20702266262765	26.888401779519377	29.513054731802374	23.3915208260506
45	20.32868194650604	25.60423225554187	29.638114100470382	24.428971697481707
46	21.405021287718363	26.035618013224205	29.245935570518288	23.31342512853914
47	19.589057252115225	26.20625976860298	30.92632122513555	23.278361754146243
48	19.080957081367217	26.625107713623606	30.553161919475368	23.74077328553381
49	20.770480463856565	25.282286727025255	31.30638695302464	22.640845856093534
50	19.32448815442335	24.826994123165946	31.906820676340615	23.941697046070086
51	19.711247799241992	24.376695393841384	30.550611855883158	25.36144495103347
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	252.0
1	535.5
2	819.0
3	9512.5
4	18206.0
5	12122.0
6	6038.0
7	5310.5
8	4583.0
9	4407.5
10	4232.0
11	4069.5
12	3907.0
13	3849.0
14	3791.0
15	3634.5
16	3478.0
17	3366.0
18	3254.0
19	3430.5
20	3607.0
21	3859.5
22	4112.0
23	4834.5
24	5557.0
25	6468.0
26	8945.5
27	10512.0
28	12148.0
29	13784.0
30	17450.5
31	21117.0
32	24286.0
33	27455.0
34	30207.5
35	32960.0
36	35276.5
37	37593.0
38	41376.5
39	45160.0
40	47886.5
41	50613.0
42	55182.0
43	59751.0
44	65363.5
45	70976.0
46	80832.5
47	90689.0
48	91698.0
49	92707.0
50	87788.0
51	82869.0
52	72055.5
53	61242.0
54	52989.5
55	44737.0
56	40383.5
57	36030.0
58	32869.0
59	29708.0
60	26138.5
61	22569.0
62	19990.0
63	17411.0
64	14078.5
65	10746.0
66	8306.5
67	5867.0
68	4691.0
69	3515.0
70	2627.0
71	1739.0
72	1357.0
73	975.0
74	779.5
75	427.5
76	271.0
77	208.0
78	145.0
79	130.5
80	116.0
81	83.0
82	50.0
83	33.0
84	16.0
85	14.5
86	13.0
87	9.0
88	5.0
89	4.0
90	3.0
91	3.5
92	4.0
93	4.0
94	4.0
95	3.0
96	2.0
97	1.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	941153.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.91912916628484
#Duplication Level	Percentage of deduplicated	Percentage of total
1	75.28676934623438	26.289484233134523
2	9.070018223304064	6.334342757602239
3	3.3957094622721353	3.5572565197276904
4	1.9586859400692103	2.735824293498512
5	1.2947248370655684	2.260533191014484
6	0.9867208751099732	2.0673260213420854
7	0.7590471647442657	1.8553686192305112
8	0.625368010126166	1.7469845057646496
9	0.5332802136706588	1.6759512596690742
>10	5.906738165228012	44.03140872348311
>50	0.15307043502364956	3.2326898528392705
>100	0.024578321302111027	1.4713073649346853
>500	0.003422297902811322	0.9036649679905223
>1k	0.001555589955823328	0.747767008256644
>5k	0.0	0.0
>10k+	3.111179911646656E-4	1.0900906815120102
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	10034	1.0661390868434781	No Hit
CGTTATTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1665	0.17691066170962638	No Hit
GAATCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTC	1511	0.16054775365960688	No Hit
GCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	1397	0.14843495159660544	No Hit
CGCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTG	1224	0.13005324320275236	No Hit
CTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGCT	1086	0.11539037754754009	No Hit
CCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTTCTGC	978	0.10391509138259136	No Hit
CGTTTCTGTCTCTTATACACATCTGACGCATACCGCATCGTATGCCGTCTT	973	0.1033838281342141	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.250105987018051E-4	0.0	0.0	0.1539600893797289	0.0
2	4.250105987018051E-4	0.0	0.0	0.6187091790601528	0.0
3	4.250105987018051E-4	0.0	0.0	0.9889996631791005	0.0
4	4.250105987018051E-4	0.0	0.0	1.3918034580987364	0.0
5	4.250105987018051E-4	0.0	0.0	2.524031693040345	0.0
6	4.250105987018051E-4	0.0	0.0	3.6502035269504534	0.0
7	4.250105987018051E-4	0.0	0.0	4.584058064947994	0.0
8	4.250105987018051E-4	0.0	0.0	5.929535367788234	0.0
9	4.250105987018051E-4	0.0	0.0	6.536344250084737	0.0
10	4.250105987018051E-4	0.0	0.0	7.573795121515843	0.0
11	4.250105987018051E-4	0.0	0.0	8.638234165964514	0.0
12	4.250105987018051E-4	0.0	0.0	9.648059348480002	0.0
13	4.250105987018051E-4	0.0	0.0	10.169759858386469	0.0
14	4.250105987018051E-4	0.0	0.0	10.398309307838364	0.0
15	4.250105987018051E-4	0.0	0.0	10.754680694849828	0.0
16	4.250105987018051E-4	0.0	0.0	11.261930844400432	0.0
17	4.250105987018051E-4	0.0	0.0	11.943647844718127	0.0
18	4.250105987018051E-4	0.0	0.0	12.703354289897604	0.0
19	4.250105987018051E-4	0.0	1.0625264967545128E-4	13.15078419768093	0.0
20	4.250105987018051E-4	0.0	1.0625264967545128E-4	13.602464211451274	0.0
21	4.250105987018051E-4	0.0	1.0625264967545128E-4	14.107589308008368	0.0
22	4.250105987018051E-4	0.0	1.0625264967545128E-4	14.676891004969436	0.0
23	4.250105987018051E-4	0.0	1.0625264967545128E-4	15.2332298786701	0.0
24	4.250105987018051E-4	0.0	1.0625264967545128E-4	15.666953194645291	0.0
25	4.250105987018051E-4	0.0	1.0625264967545128E-4	16.053712839463934	0.0
26	4.250105987018051E-4	0.0	1.0625264967545128E-4	16.41752191195268	0.0
27	4.250105987018051E-4	0.0	1.0625264967545128E-4	16.789937449065135	0.0
28	4.250105987018051E-4	0.0	1.0625264967545128E-4	17.174465788240596	0.0
29	4.250105987018051E-4	0.0	1.0625264967545128E-4	17.580032152051793	0.0
30	4.250105987018051E-4	0.0	1.0625264967545128E-4	18.09046988109266	0.0
31	4.250105987018051E-4	0.0	1.0625264967545128E-4	18.512186647654527	0.0
32	4.250105987018051E-4	0.0	1.0625264967545128E-4	18.934859688063472	0.0
33	4.250105987018051E-4	0.0	1.0625264967545128E-4	19.371345572930224	0.0
34	4.250105987018051E-4	0.0	1.0625264967545128E-4	19.802625077962883	0.0
35	4.250105987018051E-4	0.0	1.0625264967545128E-4	20.24367982676568	0.0
36	4.250105987018051E-4	0.0	1.0625264967545128E-4	20.644358568691807	0.0
37	4.250105987018051E-4	0.0	1.0625264967545128E-4	21.048968658655927	0.0
38	5.312632483772564E-4	0.0	1.0625264967545128E-4	21.484392017025925	0.0
39	5.312632483772564E-4	0.0	1.0625264967545128E-4	21.956685044833307	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
GAGTACG	35	1.2116107E-7	45.000004	1
CGACGTC	20	7.032636E-4	45.0	18
CCGCGTC	20	7.032636E-4	45.0	39
ACGTACG	20	7.032636E-4	45.0	1
CGTTTTT	4220	0.0	43.88033	1
CGTTATT	875	0.0	43.45714	1
AATTGCG	75	0.0	42.0	1
CGTAAGG	260	0.0	40.673077	2
AGTACGG	220	0.0	39.886364	2
CTTACGG	85	0.0	39.705883	2
TGCGAAG	80	0.0	39.375	1
TAATGCG	40	3.457335E-7	39.375	1
CACAACG	180	0.0	38.75	12
CTATGCG	35	6.247081E-6	38.57143	1
ATTCACG	35	6.247081E-6	38.57143	12
GTATACG	35	6.247081E-6	38.57143	1
TTCGCAG	70	0.0	38.57143	1
GCGTACG	35	6.247081E-6	38.57143	1
TAGTACG	65	9.094947E-12	38.07692	1
TACGGGA	560	0.0	37.76786	4
>>END_MODULE