##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936774.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	458719
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.83858963766489	31.0	31.0	34.0	30.0	34.0
2	31.924413420852417	33.0	31.0	34.0	30.0	34.0
3	31.877696367492952	33.0	31.0	34.0	30.0	34.0
4	35.60135943791297	37.0	35.0	37.0	33.0	37.0
5	30.6158715902328	37.0	35.0	37.0	0.0	37.0
6	33.058353807014754	37.0	35.0	37.0	17.0	37.0
7	35.11598385939976	37.0	35.0	37.0	32.0	37.0
8	35.58092426954192	37.0	35.0	37.0	33.0	37.0
9	37.61281525291082	39.0	37.0	39.0	35.0	39.0
10	37.20025549410423	39.0	37.0	39.0	34.0	39.0
11	37.05829058748384	39.0	37.0	39.0	33.0	39.0
12	36.99552449320826	39.0	37.0	39.0	33.0	39.0
13	36.77244674844513	39.0	35.0	39.0	33.0	39.0
14	37.91135967771119	40.0	37.0	41.0	33.0	41.0
15	38.00528210080681	40.0	37.0	41.0	33.0	41.0
16	38.02641704398553	40.0	37.0	41.0	33.0	41.0
17	38.00441228726083	40.0	36.0	41.0	33.0	41.0
18	37.93809499933511	40.0	36.0	41.0	33.0	41.0
19	37.89953544544699	40.0	36.0	41.0	33.0	41.0
20	37.92293757180322	40.0	36.0	41.0	33.0	41.0
21	37.86783630065465	40.0	36.0	41.0	33.0	41.0
22	37.8923523987452	40.0	36.0	41.0	33.0	41.0
23	37.8865645416911	40.0	36.0	41.0	33.0	41.0
24	37.79650940990018	39.0	36.0	41.0	33.0	41.0
25	37.65663946773515	39.0	36.0	41.0	33.0	41.0
26	37.60494551130431	39.0	36.0	41.0	33.0	41.0
27	37.548146032756435	39.0	36.0	41.0	33.0	41.0
28	37.52185978780037	39.0	36.0	41.0	33.0	41.0
29	37.54340674792193	39.0	36.0	41.0	33.0	41.0
30	37.4391076018216	39.0	36.0	41.0	32.0	41.0
31	37.3956147445386	39.0	36.0	41.0	32.0	41.0
32	37.34039139429586	39.0	36.0	41.0	32.0	41.0
33	37.24760692275663	39.0	35.0	41.0	32.0	41.0
34	37.22323906356615	39.0	35.0	41.0	32.0	41.0
35	37.14162046917612	39.0	35.0	41.0	32.0	41.0
36	37.00156740836983	39.0	35.0	41.0	31.0	41.0
37	36.89202976113917	39.0	35.0	41.0	31.0	41.0
38	36.87988289126895	39.0	35.0	41.0	31.0	41.0
39	36.795890294494015	39.0	35.0	41.0	31.0	41.0
40	36.67114290011968	39.0	35.0	41.0	30.0	41.0
41	36.5947475469732	39.0	35.0	40.0	30.0	41.0
42	36.53528412819177	39.0	35.0	40.0	30.0	41.0
43	36.40638604461555	39.0	35.0	40.0	30.0	41.0
44	36.271610724648426	39.0	35.0	40.0	30.0	41.0
45	36.27310837353587	39.0	35.0	40.0	30.0	41.0
46	36.026994739699035	38.0	35.0	40.0	29.0	41.0
47	35.93666492994622	38.0	35.0	40.0	29.0	41.0
48	35.83597801704311	38.0	35.0	40.0	28.0	41.0
49	35.700243504193196	38.0	35.0	40.0	28.0	41.0
50	35.48307133561069	38.0	34.0	40.0	27.0	41.0
51	33.431791576106505	36.0	31.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	12.0
9	25.0
10	22.0
11	19.0
12	5.0
13	2.0
14	4.0
15	12.0
16	11.0
17	48.0
18	98.0
19	192.0
20	319.0
21	568.0
22	863.0
23	1319.0
24	1812.0
25	2554.0
26	3393.0
27	4389.0
28	5383.0
29	6919.0
30	8805.0
31	11262.0
32	14805.0
33	21036.0
34	31281.0
35	40199.0
36	39686.0
37	59764.0
38	90488.0
39	113406.0
40	17.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	40.94075021963337	21.30127594453249	27.571127422234525	10.18684641359961
2	28.55190214488609	27.319121292120013	27.410462614367404	16.7185139486265
3	28.94255524624007	27.355309023607045	28.212260665025866	15.489875065127018
4	27.47259215336622	25.18622511820962	29.781195023532923	17.55998770489123
5	22.028736546774823	38.41153298642524	23.04526300414851	16.514467462651428
6	23.27089132998633	36.87987635131748	25.20802495645482	14.641207362241374
7	78.45238588329676	4.942241328569342	11.293188204543522	5.31218458359039
8	78.84521896847525	6.219929848120527	8.691159511596425	6.243691671807795
9	74.09677820190575	5.565280705617164	10.69848861721446	9.639452475262633
10	37.21428586999884	25.087253852576413	20.027729394247896	17.670730883176848
11	27.22407399737094	28.374669459952607	24.55315781556901	19.848098727107445
12	25.794658603633163	22.624744124398596	30.85352906681432	20.727068205153916
13	21.83449998801009	25.627235845910022	34.18171037170904	18.356553794370846
14	18.73499898630752	30.227655710794625	29.550770733281155	21.486574569616693
15	16.68363420743418	26.913426302376838	37.83863323734138	18.564306252847604
16	18.438085189407893	26.698043900514257	32.37188780059252	22.49198310948533
17	18.38184160673528	26.085904442589037	29.547718755926834	25.984535194748855
18	19.27280099581661	26.667306128588525	32.383223716480025	21.67666915911484
19	20.035577336016168	28.62514960138996	28.47952668191202	22.859746380681855
20	21.63633945836122	27.84842136471347	30.248147558745114	20.267091618180196
21	20.49773390681441	30.001155391426998	29.67350382260164	19.827606879156956
22	19.886248444036543	25.060222053152366	27.852999330744964	27.200530172066124
23	18.852064117684247	28.152093111469114	29.615516252869405	23.380326517977238
24	21.46978869416789	24.44895458875695	29.77618106073653	24.30507565633863
25	18.285268323309044	29.58913844859271	27.6280250000545	24.497568228043747
26	17.293811679917333	28.293138064915556	28.66046533934718	25.752584915819927
27	19.91393423860795	29.662603903479035	28.02870602700128	22.394755830911734
28	16.082394668631558	26.564410892071184	33.62210852395475	23.731085915342508
29	17.772754126164383	25.255548603829357	29.43130761969746	27.540389650308793
30	19.378748209688286	28.738508760264995	28.8584078706136	23.024335159433118
31	19.606992516115533	26.958769965926855	28.766412553218856	24.66782496473876
32	20.285839479071065	27.607969148868918	27.149518550572356	24.956672821487665
33	19.589988642284275	25.616771923552328	27.255683762826482	27.537555671336918
34	17.83575565869301	23.459241932424863	32.40633154501993	26.29867086386219
35	16.59752484636564	24.748702364628457	28.534026277525022	30.119746511480884
36	20.986922277036705	24.390095025494908	29.087088173805753	25.535894523662634
37	18.033044194812074	25.648381689007866	35.18559292290051	21.132981193279544
38	18.265648468888358	24.835683719226804	29.50935104061528	27.389316771269556
39	21.6014597171689	21.7917723050495	31.973822754235165	24.63294522354644
40	19.690267898212195	21.68800507500234	31.73053655941873	26.891190467366734
41	18.992891072748243	24.51522609702236	28.188062844573693	28.303819985655704
42	21.416161092084696	22.888304168783066	29.748277267782676	25.94725747134956
43	23.515049518332575	22.86563233700806	28.82156614397921	24.797752000680156
44	19.881670478005052	23.425452183144802	30.18972399224798	26.503153346602172
45	19.187345629895425	22.226461079658787	28.890235634451596	29.695957655994192
46	22.758377132841677	23.77119761771368	28.316245893455473	25.154179355989182
47	17.650675031991263	22.644145980436825	34.548165652610855	25.157013334961054
48	19.29438283567936	21.60821766702491	30.760879754272224	28.33651974302351
49	20.002441581883463	19.930284117291848	34.78294991051166	25.284324390313024
50	19.65996612305137	19.775069268986023	32.84494429051337	27.720020317449247
51	17.968952670371184	20.70025440411232	28.937323285061222	32.39346964045527
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	255.0
1	324.5
2	394.0
3	635.5
4	877.0
5	633.5
6	390.0
7	354.0
8	318.0
9	342.0
10	366.0
11	381.0
12	396.0
13	406.0
14	416.0
15	462.0
16	508.0
17	474.5
18	441.0
19	569.5
20	698.0
21	887.0
22	1076.0
23	1348.0
24	1620.0
25	1879.5
26	2758.0
27	3377.0
28	4315.0
29	5253.0
30	6264.5
31	7276.0
32	8853.5
33	10431.0
34	10702.0
35	10973.0
36	12073.5
37	13174.0
38	15313.0
39	17452.0
40	19303.0
41	21154.0
42	26165.5
43	31177.0
44	36215.5
45	41254.0
46	54186.5
47	67119.0
48	64229.0
49	61339.0
50	57386.5
51	53434.0
52	43718.5
53	34003.0
54	27725.5
55	21448.0
56	17951.0
57	14454.0
58	12962.0
59	11470.0
60	10357.0
61	9244.0
62	7824.5
63	6405.0
64	5207.5
65	4010.0
66	3073.0
67	2136.0
68	1564.5
69	993.0
70	762.0
71	531.0
72	449.0
73	367.0
74	278.0
75	123.5
76	58.0
77	46.5
78	35.0
79	30.0
80	25.0
81	21.5
82	18.0
83	15.5
84	13.0
85	9.5
86	6.0
87	4.5
88	3.0
89	2.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	458719.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	33.93122037685025
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.84199470209761	24.037553341725314
2	12.035191400793824	8.167374633958165
3	4.9757707441617125	5.0650192099450555
4	2.653930628830591	3.6020442012689418
5	1.6224229368230128	2.7525395106899113
6	1.112296793919211	2.2644952583362037
7	0.7821303748715714	1.8577046679237048
8	0.6008724593746999	1.631066866993835
9	0.5057345201213644	1.5444170508976758
>10	4.478212581390899	34.356351067102416
>50	0.3286825977994496	6.873339621462635
>100	0.05434909097471214	3.3091174177304934
>500	0.004529090914559345	1.0199800652010795
>1k	0.003882077926765153	3.5189970867645513
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGC	4030	0.8785334812815688	No Hit
GAATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTC	3751	0.8177119325774603	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCG	3531	0.7697522884380198	No Hit
GCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	2202	0.48003243815930885	No Hit
CCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	1261	0.27489596027197477	No Hit
CTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGCT	1254	0.27336997159481075	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCT	824	0.1796306671404498	No Hit
TCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCTGC	798	0.17396270919669776	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTC	646	0.14082695506399343	No Hit
GAACTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTCT	614	0.13385100682552936	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCG	600	0.1307990294712013	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGC	593	0.1292730407940373	No Hit
GAATATGTCTCTTATACACATCTGACGCCATTCTCGTCGTATGCCGTCTTC	571	0.12447707638009325	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.3466174280986835	0.0
2	0.0	0.0	0.0	1.7228412165181735	0.0
3	0.0	0.0	0.0	2.273505130591931	0.0
4	0.0	0.0	0.0	3.142010686280708	0.0
5	0.0	0.0	0.0	6.016319358910357	0.0
6	0.0	0.0	0.0	7.413034995280335	0.0
7	0.0	0.0	0.0	8.949705593184499	0.0
8	0.0	0.0	0.0	11.29406019807333	0.0
9	0.0	0.0	0.0	12.029804738848838	0.0
10	0.0	0.0	0.0	14.705516885064712	0.0
11	0.0	0.0	0.0	17.103063095271832	0.0
12	0.0	0.0	0.0	19.910010267723813	0.0
13	0.0	0.0	0.0	20.731210174420507	0.0
14	0.0	0.0	0.0	21.053411783684567	0.0
15	0.0	0.0	0.0	21.681465123528785	0.0
16	2.179983824520022E-4	0.0	0.0	22.937135806452318	0.0
17	2.179983824520022E-4	0.0	0.0	24.307909635310505	0.0
18	2.179983824520022E-4	0.0	0.0	25.9402815231111	0.0
19	2.179983824520022E-4	0.0	0.0	26.84737279249388	0.0
20	2.179983824520022E-4	0.0	0.0	27.750758089374976	0.0
21	2.179983824520022E-4	0.0	0.0	28.855355893259272	0.0
22	2.179983824520022E-4	0.0	0.0	29.92463795918634	0.0
23	2.179983824520022E-4	0.0	0.0	31.015937861741065	0.0
24	2.179983824520022E-4	0.0	0.0	31.875287485366858	0.0
25	2.179983824520022E-4	0.0	0.0	32.60187609407938	0.0
26	4.359967649040044E-4	0.0	0.0	33.28530102306641	0.0
27	4.359967649040044E-4	0.0	0.0	33.90703240981952	0.0
28	6.539951473560066E-4	0.0	0.0	34.57149147953322	0.0
29	6.539951473560066E-4	0.0	0.0	35.25295442307818	0.0
30	6.539951473560066E-4	0.0	0.0	36.02205271636885	0.0
31	6.539951473560066E-4	0.0	0.0	36.77632711965277	0.0
32	6.539951473560066E-4	0.0	0.0	37.421166334945795	0.0
33	8.719935298080088E-4	0.0	0.0	38.09172935936815	0.0
34	8.719935298080088E-4	0.0	0.0	38.740928542310215	0.0
35	8.719935298080088E-4	0.0	0.0	39.39884766055036	0.0
36	8.719935298080088E-4	0.0	0.0	40.027554995541934	0.0
37	8.719935298080088E-4	0.0	0.0	40.651466366119564	0.0
38	8.719935298080088E-4	0.0	0.0	41.282135686553204	0.0
39	8.719935298080088E-4	0.0	0.0	41.90735504742555	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGAC	80	0.0	45.000004	25
TATCACG	20	7.0288486E-4	45.000004	1
AATCCGT	20	7.0288486E-4	45.000004	16
ATCTCGG	20	7.0288486E-4	45.000004	2
GTACCGA	20	7.0288486E-4	45.000004	9
GAAGCGT	20	7.0288486E-4	45.000004	29
ACGCCGA	35	1.2097917E-7	45.000004	12
GATCGGC	20	7.0288486E-4	45.000004	35
CTATCTC	70	0.0	45.000004	3
CGACGGT	80	0.0	45.000004	28
GGCACGT	20	7.0288486E-4	45.000004	8
CGCATCG	20	7.0288486E-4	45.000004	21
GTCTACG	20	7.0288486E-4	45.000004	1
TCGATTA	20	7.0288486E-4	45.000004	41
CCGCTCG	35	1.2097917E-7	45.000004	19
AGACGAC	20	7.0288486E-4	45.000004	16
CCGTAGA	20	7.0288486E-4	45.000004	17
GTTCGCG	20	7.0288486E-4	45.000004	1
ACGTGAC	35	1.2097917E-7	45.000004	15
CGAATTC	20	7.0288486E-4	45.000004	45
>>END_MODULE