FastQCFastQC Report
Sat 14 Jan 2017
SRR2936772.fastq

Summary

[OK]Basic Statistics

MeasureValue
FilenameSRR2936772.fastq
File typeConventional base calls
EncodingSanger / Illumina 1.9
Total Sequences277685
Sequences flagged as poor quality0
Sequence length51
%GC47

[OK]Per base sequence quality

Per base quality graph

[OK]Per sequence quality scores

Per Sequence quality graph

[FAIL]Per base sequence content

Per base sequence content

[FAIL]Per sequence GC content

Per sequence GC content graph

[OK]Per base N content

N content graph

[OK]Sequence Length Distribution

Sequence length distribution

[FAIL]Sequence Duplication Levels

Duplication level graph

[FAIL]Overrepresented sequences

SequenceCountPercentagePossible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGC48181.7350595098762986No Hit
GAATCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTC45881.6522318454363756No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCG41441.4923384410393072No Hit
GCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC20690.7450888596791329TruSeq Adapter, Index 13 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCT10680.3846084592253813No Hit
CCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC9400.3385130633631633TruSeq Adapter, Index 13 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGCT8810.3172659668329222TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTC8010.28845634441903595No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGC7610.2740515332120928No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCG7270.2618074436861912No Hit
GAATATGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTC6910.24884311359994238No Hit
GAACTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCT6690.2409204674361237No Hit
TCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC5620.2023875974575508TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCATAACCCTCGTA4660.16781605056088733No Hit
AGACAGGAAAAGCCGTGTGTATTTTTATAGCAAAGAACAGATGAAGATATT4430.15953328411689505No Hit
GCTGACTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC4020.14476835262977836TruSeq Adapter, Index 13 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC3240.11667897077623926TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT3020.10875632461242056No Hit

[FAIL]Adapter Content

Adapter graph

[WARN]Kmer Content

Kmer graph

SequenceCountPValueObs/Exp MaxMax Obs/Exp Position
TCGTTCA253.8831386E-545.00000416
CTCGTTC253.8831386E-545.00000415
ATTAGCC253.8831386E-545.00000425
ATCTAGT253.8831386E-545.00000412
GCGAAAG253.8831386E-545.0000041
GCGCGAC253.8831386E-545.0000049
CGATTGG502.1827873E-1145.0000042
CGCGACC253.8831386E-545.00000410
CCTAACA253.8831386E-545.00000427
AAACACG253.8831386E-545.00000440
GCGATGC253.8831386E-545.0000049
GGGTTAC253.8831386E-545.0000047
AGCCTAA253.8831386E-545.00000426
TCTTTAC253.8831386E-545.00000421
CGCACGG253.8831386E-545.0000042
TCACGGG453.8198777E-1045.03
AGCACAT207.0240354E-445.041
TCACGAC207.0240354E-445.025
AACGTTA207.0240354E-445.040
GTCATAC207.0240354E-445.039
Produced by FastQC (version 0.11.5)