##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936772.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	277685
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.739888722833427	31.0	31.0	34.0	30.0	34.0
2	31.804328645767686	31.0	31.0	34.0	30.0	34.0
3	31.78342726470641	31.0	31.0	34.0	30.0	34.0
4	35.523791346309665	37.0	35.0	37.0	33.0	37.0
5	30.63519095377856	35.0	35.0	37.0	0.0	37.0
6	33.00633091452545	36.0	35.0	37.0	17.0	37.0
7	34.96076849667789	37.0	35.0	37.0	32.0	37.0
8	35.33078848335344	37.0	35.0	37.0	33.0	37.0
9	37.36201091164449	39.0	37.0	39.0	34.0	39.0
10	37.180402254352956	39.0	37.0	39.0	34.0	39.0
11	36.99689936438771	39.0	37.0	39.0	33.0	39.0
12	36.8537227433963	39.0	35.0	39.0	33.0	39.0
13	36.618909915911914	39.0	35.0	39.0	33.0	39.0
14	37.75686839404361	39.0	36.0	41.0	33.0	41.0
15	37.756843185624	39.0	36.0	41.0	33.0	41.0
16	37.798750382627794	39.0	36.0	41.0	33.0	41.0
17	37.743515134054775	39.0	36.0	41.0	33.0	41.0
18	37.65031960674865	39.0	36.0	41.0	33.0	41.0
19	37.626955002970995	39.0	36.0	41.0	33.0	41.0
20	37.65769126888381	39.0	36.0	41.0	33.0	41.0
21	37.639883321029224	39.0	36.0	41.0	33.0	41.0
22	37.64650233177881	39.0	36.0	41.0	33.0	41.0
23	37.69693357581432	39.0	36.0	41.0	33.0	41.0
24	37.56667807047554	39.0	36.0	41.0	33.0	41.0
25	37.42443416100978	39.0	35.0	41.0	32.0	41.0
26	37.34271566703279	39.0	35.0	41.0	32.0	41.0
27	37.22293606064426	39.0	35.0	41.0	32.0	41.0
28	37.20549183427265	39.0	35.0	41.0	32.0	41.0
29	37.19721987143706	39.0	35.0	41.0	32.0	41.0
30	37.0763527018024	39.0	35.0	41.0	32.0	41.0
31	37.04464411113312	39.0	35.0	40.0	31.0	41.0
32	36.89584241136539	39.0	35.0	40.0	31.0	41.0
33	36.8124529592884	39.0	35.0	40.0	31.0	41.0
34	36.7085078416191	39.0	35.0	40.0	31.0	41.0
35	36.69863694473954	39.0	35.0	40.0	31.0	41.0
36	36.64000216072168	39.0	35.0	40.0	31.0	41.0
37	36.52205196535643	39.0	35.0	40.0	30.0	41.0
38	36.49934278048868	39.0	35.0	40.0	30.0	41.0
39	36.357894736842105	38.0	35.0	40.0	30.0	41.0
40	36.19702900768857	38.0	35.0	40.0	30.0	41.0
41	36.21355852854853	38.0	35.0	40.0	30.0	41.0
42	36.14993607865027	38.0	35.0	40.0	30.0	41.0
43	36.09707042152079	38.0	35.0	40.0	30.0	41.0
44	35.89759619712984	38.0	35.0	40.0	30.0	41.0
45	35.887484019662566	38.0	35.0	40.0	30.0	41.0
46	35.61181554639249	38.0	35.0	40.0	28.0	41.0
47	35.42804616741992	38.0	34.0	40.0	27.0	41.0
48	35.38131695986459	38.0	34.0	40.0	27.0	41.0
49	35.28413490105695	38.0	34.0	40.0	27.0	41.0
50	35.06940958280065	38.0	34.0	40.0	26.0	41.0
51	33.104197201865425	36.0	31.0	39.0	21.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	1.0
8	8.0
9	9.0
10	10.0
11	10.0
12	10.0
13	5.0
14	6.0
15	14.0
16	16.0
17	32.0
18	66.0
19	136.0
20	225.0
21	387.0
22	616.0
23	930.0
24	1289.0
25	1700.0
26	2211.0
27	2769.0
28	3731.0
29	4439.0
30	5858.0
31	7778.0
32	10265.0
33	14280.0
34	21154.0
35	26241.0
36	25873.0
37	37529.0
38	52871.0
39	57207.0
40	9.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	46.419864234654376	20.39649242847111	23.277814790139907	9.90582854673461
2	29.760339953544484	30.359940220033494	23.166177503286097	16.713542323135925
3	30.234258242252913	30.309163260529015	23.810072564236457	15.646505932981617
4	27.430361740821436	22.608711309577398	31.121594612600607	18.83933233700056
5	23.9184687685687	37.07402272358968	20.425302050884998	18.582206456956623
6	24.323964204044152	35.48013036354142	26.56031114392207	13.635594288492356
7	71.69346561751625	6.557070061400508	16.092334839836507	5.657129481246736
8	67.34645371554099	10.850784161910077	13.48902533446171	8.31373678808722
9	61.17003079028396	8.157084466211714	15.054468192376252	15.618416551128078
10	39.49367088607595	21.225489313430685	23.242162882402724	16.03867691809064
11	29.91807263626051	22.823703116841024	25.68737958478132	21.570844662117146
12	26.09431550137746	20.12892306030214	32.82928498118372	20.94747645713668
13	21.56616309847489	24.576408520445828	36.39051443182023	17.46691394925905
14	18.874984244737743	29.40454111673299	29.302266957163692	22.418207681365576
15	15.934962277400652	23.81151304535715	42.66561031384482	17.587914363397374
16	17.29333597421539	26.738570682607993	31.82310891837874	24.144984424797883
17	18.005293768118552	24.6095395862218	31.04704971460468	26.338116931054973
18	19.17280371644129	26.159137151808704	32.82244269586042	21.845616435889585
19	18.95529106721645	27.948574823991212	28.33102256153555	24.765111547256787
20	20.59599906368727	28.87732502655887	31.186056142751678	19.340619767002178
21	19.458379098618938	30.770477339431366	31.203702036480184	18.56744152546951
22	17.051695266218918	24.528512523182744	29.96236743072186	28.45742477987648
23	17.447827574409853	28.72787511028684	31.17021085042404	22.65408646487927
24	22.999801933845905	22.33934134000756	28.5143237841439	26.146532942002633
25	16.109620613284836	31.528890649476928	26.640617966400775	25.72087077083746
26	16.490627869708483	25.890487422799215	29.721086843005562	27.89779786448674
27	20.711957793903164	29.286421664836055	26.628733997155052	23.372886544105732
28	13.306084232133534	26.371608117111116	33.49514737922466	26.827160271530694
29	18.12989538505861	26.464519149395898	26.799791130237498	28.605794335307994
30	18.396024272106885	31.228910456092336	28.162126150134142	22.212939121666636
31	20.214271566703278	27.064839656445255	26.301024542197094	26.419864234654373
32	19.699659686335234	32.69892143976088	22.493472819921852	25.107946053982026
33	16.866953562489872	30.54684264544358	23.437348074256803	29.148855717809745
34	17.393449412103642	24.595134775014856	27.729981813925853	30.281433998955652
35	14.64249059185768	28.623080108756326	23.37252642382556	33.36190287556044
36	17.33186884419396	27.194122837027567	25.84367178637665	29.630336532401824
37	16.01022741595693	29.87809928516124	29.382573779642403	24.729099519239426
38	15.45672254533014	30.43952680195185	25.452221041827972	28.651529610890037
39	19.309649422907253	27.468534490519836	25.909213677368243	27.312602409204672
40	18.806921511784935	24.77591515566199	28.556817977204386	27.860345355348688
41	15.714208545654248	25.337702792732774	23.398455083998055	35.54963357761493
42	17.970722221221887	21.894952914273365	29.474764571366833	30.65956029313791
43	21.33100455552154	23.579595584925364	25.564218448961952	29.525181410591138
44	18.379458739218897	24.329366008246755	28.8006194068819	28.490555845652448
45	18.537911662495272	23.06138250175559	26.404739182887084	31.995966652862055
46	23.986891621801682	25.14215748059852	25.4122476907287	25.458703206871096
47	14.872247330608424	22.507517510848622	35.06491168050129	27.555323478041664
48	18.3535300790464	22.91193258548355	28.598952049984693	30.13558528548535
49	18.73489745575022	19.71730558006374	34.5999963987972	26.947800565388842
50	19.08709508975998	19.609989736572015	31.74604317842159	29.556871995246414
51	17.029367808848157	19.640960080666943	26.37232835767146	36.957343752813436
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	143.0
1	194.5
2	246.0
3	436.5
4	627.0
5	428.5
6	230.0
7	250.0
8	270.0
9	278.5
10	287.0
11	304.0
12	321.0
13	333.0
14	345.0
15	359.0
16	373.0
17	359.5
18	346.0
19	472.0
20	598.0
21	634.5
22	671.0
23	880.5
24	1090.0
25	1327.5
26	1840.0
27	2115.0
28	2497.0
29	2879.0
30	3336.0
31	3793.0
32	4743.5
33	5694.0
34	5835.5
35	5977.0
36	7021.0
37	8065.0
38	8755.5
39	9446.0
40	10715.5
41	11985.0
42	14235.5
43	16486.0
44	20521.0
45	24556.0
46	35175.0
47	45794.0
48	40831.5
49	35869.0
50	34363.0
51	32857.0
52	26002.5
53	19148.0
54	15315.5
55	11483.0
56	9754.5
57	8026.0
58	7439.5
59	6853.0
60	6563.0
61	6273.0
62	5351.5
63	4430.0
64	3869.5
65	3309.0
66	2679.5
67	2050.0
68	1678.5
69	1307.0
70	1128.0
71	949.0
72	744.5
73	540.0
74	434.0
75	244.5
76	161.0
77	131.5
78	102.0
79	69.0
80	36.0
81	35.0
82	34.0
83	26.0
84	18.0
85	10.5
86	3.0
87	2.5
88	2.0
89	2.0
90	2.0
91	1.5
92	1.0
93	0.5
94	0.0
95	0.5
96	1.0
97	1.0
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	277685.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.17474836595423
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.69388277450729	25.218142859715144
2	11.578192986946506	8.145200496965986
3	4.937803941643205	5.2105803338315
4	2.676222165344254	3.7654176494949314
5	1.651394932173023	2.9043700595999065
6	1.1179933452777067	2.359508075697283
7	0.79037624776043	1.9460899940580152
8	0.6398771435884311	1.80060140086789
9	0.4648067571026363	1.4714514647892396
>10	4.179165600204761	31.296252948484792
>50	0.2057844893780394	4.567765633721663
>100	0.05119017148707448	3.133046437510129
>500	0.008190427437931917	2.1722455300070225
>1k	0.005119017148707448	6.009327115256496
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGC	4818	1.7350595098762986	No Hit
GAATCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTC	4588	1.6522318454363756	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCG	4144	1.4923384410393072	No Hit
GCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC	2069	0.7450888596791329	TruSeq Adapter, Index 13 (95% over 23bp)
GAATGACTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCT	1068	0.3846084592253813	No Hit
CCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC	940	0.3385130633631633	TruSeq Adapter, Index 13 (95% over 23bp)
CTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGCT	881	0.3172659668329222	TruSeq Adapter, Index 16 (95% over 22bp)
GAATGATCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTC	801	0.28845634441903595	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGC	761	0.2740515332120928	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCG	727	0.2618074436861912	No Hit
GAATATGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTC	691	0.24884311359994238	No Hit
GAACTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCT	669	0.2409204674361237	No Hit
TCTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC	562	0.2023875974575508	TruSeq Adapter, Index 13 (95% over 23bp)
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCCATAACCCTCGTA	466	0.16781605056088733	No Hit
AGACAGGAAAAGCCGTGTGTATTTTTATAGCAAAGAACAGATGAAGATATT	443	0.15953328411689505	No Hit
GCTGACTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC	402	0.14476835262977836	TruSeq Adapter, Index 13 (95% over 23bp)
ACTGTCTCTTATACACATCTGACGCCATAACCCTCGTATGCCGTCTTCTGC	324	0.11667897077623926	TruSeq Adapter, Index 16 (95% over 21bp)
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	302	0.10875632461242056	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	3.60120280173578E-4	0.0	0.0	0.41918000612204476	0.0
2	3.60120280173578E-4	0.0	0.0	2.2446297063219114	0.0
3	3.60120280173578E-4	0.0	0.0	2.875920557466194	0.0
4	3.60120280173578E-4	0.0	0.0	3.9948142679655003	0.0
5	3.60120280173578E-4	0.0	0.0	8.260078866341358	0.0
6	3.60120280173578E-4	0.0	0.0	9.834884851540414	0.0
7	3.60120280173578E-4	0.0	0.0	11.959594504564524	0.0
8	3.60120280173578E-4	0.0	0.0	14.733240902461421	0.0
9	3.60120280173578E-4	0.0	0.0	15.64794641410231	0.0
10	3.60120280173578E-4	0.0	0.0	19.356104939049644	0.0
11	3.60120280173578E-4	0.0	0.0	21.978500819273638	0.0
12	3.60120280173578E-4	0.0	0.0	26.200550984028666	0.0
13	3.60120280173578E-4	0.0	0.0	27.225093181122496	0.0
14	3.60120280173578E-4	0.0	0.0	27.693249545348145	0.0
15	3.60120280173578E-4	0.0	0.0	28.60147289194591	0.0
16	3.60120280173578E-4	0.0	0.0	30.099573257467995	0.0
17	3.60120280173578E-4	0.0	0.0	31.592631939067648	0.0
18	3.60120280173578E-4	0.0	0.0	33.323009885301694	0.0
19	3.60120280173578E-4	0.0	0.0	34.351153285197256	0.0
20	3.60120280173578E-4	0.0	0.0	35.36093055080397	0.0
21	3.60120280173578E-4	0.0	0.0	36.624952734213224	0.0
22	3.60120280173578E-4	0.0	0.0	37.79390316365666	0.0
23	3.60120280173578E-4	0.0	0.0	38.7529034697589	0.0
24	3.60120280173578E-4	0.0	0.0	39.57325746799431	0.0
25	3.60120280173578E-4	0.0	0.0	40.27369141293192	0.0
26	3.60120280173578E-4	0.0	0.0	40.981687883753175	0.0
27	3.60120280173578E-4	0.0	0.0	41.6342258314277	0.0
28	3.60120280173578E-4	0.0	0.0	42.3415020616886	0.0
29	3.60120280173578E-4	0.0	0.0	42.99223940796226	0.0
30	3.60120280173578E-4	0.0	0.0	43.76397716837424	0.0
31	3.60120280173578E-4	0.0	0.0	44.43848245313935	0.0
32	3.60120280173578E-4	0.0	0.0	45.079136431568145	0.0
33	3.60120280173578E-4	0.0	0.0	45.742477987647874	0.0
34	3.60120280173578E-4	0.0	0.0	46.311107910041954	0.0
35	3.60120280173578E-4	0.0	0.0	46.96004465491474	0.0
36	3.60120280173578E-4	0.0	0.0	47.54812107243819	0.0
37	3.60120280173578E-4	0.0	0.0	48.10666762698742	0.0
38	3.60120280173578E-4	0.0	0.0	48.74011919981274	0.0
39	3.60120280173578E-4	0.0	0.0	49.35340403694834	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCGTTCA	25	3.8831386E-5	45.000004	16
CTCGTTC	25	3.8831386E-5	45.000004	15
ATTAGCC	25	3.8831386E-5	45.000004	25
ATCTAGT	25	3.8831386E-5	45.000004	12
GCGAAAG	25	3.8831386E-5	45.000004	1
GCGCGAC	25	3.8831386E-5	45.000004	9
CGATTGG	50	2.1827873E-11	45.000004	2
CGCGACC	25	3.8831386E-5	45.000004	10
CCTAACA	25	3.8831386E-5	45.000004	27
AAACACG	25	3.8831386E-5	45.000004	40
GCGATGC	25	3.8831386E-5	45.000004	9
GGGTTAC	25	3.8831386E-5	45.000004	7
AGCCTAA	25	3.8831386E-5	45.000004	26
TCTTTAC	25	3.8831386E-5	45.000004	21
CGCACGG	25	3.8831386E-5	45.000004	2
TCACGGG	45	3.8198777E-10	45.0	3
AGCACAT	20	7.0240354E-4	45.0	41
TCACGAC	20	7.0240354E-4	45.0	25
AACGTTA	20	7.0240354E-4	45.0	40
GTCATAC	20	7.0240354E-4	45.0	39
>>END_MODULE