##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936771.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	610005
Sequences flagged as poor quality	0
Sequence length	51
%GC	46
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.9491463184728	33.0	31.0	34.0	30.0	34.0
2	32.03218006409784	33.0	31.0	34.0	30.0	34.0
3	31.97848706158146	33.0	31.0	34.0	30.0	34.0
4	35.68036163638003	37.0	35.0	37.0	33.0	37.0
5	30.820688355013484	37.0	35.0	37.0	0.0	37.0
6	33.19168203539315	37.0	35.0	37.0	17.0	37.0
7	35.167587150924994	37.0	35.0	37.0	32.0	37.0
8	35.59880164916681	37.0	35.0	37.0	33.0	37.0
9	37.62052770059262	39.0	37.0	39.0	35.0	39.0
10	37.239491479578035	39.0	37.0	39.0	34.0	39.0
11	37.11187121417038	39.0	37.0	39.0	34.0	39.0
12	37.10857779854263	39.0	37.0	39.0	34.0	39.0
13	37.00748518454767	39.0	37.0	39.0	33.0	39.0
14	38.258989680412455	40.0	37.0	41.0	33.0	41.0
15	38.29136974287096	40.0	37.0	41.0	34.0	41.0
16	38.26801583593577	40.0	37.0	41.0	34.0	41.0
17	38.24076196096753	40.0	37.0	41.0	34.0	41.0
18	38.17397726248146	40.0	37.0	41.0	34.0	41.0
19	38.13246940598847	40.0	37.0	41.0	34.0	41.0
20	38.12505143400464	40.0	37.0	41.0	34.0	41.0
21	38.09381070646962	40.0	37.0	41.0	34.0	41.0
22	38.114710535159546	40.0	37.0	41.0	34.0	41.0
23	38.104133572675636	40.0	37.0	41.0	34.0	41.0
24	38.0716469537135	40.0	37.0	41.0	34.0	41.0
25	37.93302186047655	40.0	37.0	41.0	33.0	41.0
26	37.88222391619741	40.0	37.0	41.0	33.0	41.0
27	37.82862271620724	40.0	37.0	41.0	33.0	41.0
28	37.768201899984426	40.0	37.0	41.0	33.0	41.0
29	37.75318399029516	40.0	37.0	41.0	33.0	41.0
30	37.711161383923084	40.0	36.0	41.0	33.0	41.0
31	37.61989983688658	40.0	36.0	41.0	33.0	41.0
32	37.57175596921337	40.0	36.0	41.0	33.0	41.0
33	37.50278440340653	40.0	36.0	41.0	33.0	41.0
34	37.4128179277219	40.0	36.0	41.0	32.0	41.0
35	37.409795001680315	40.0	36.0	41.0	32.0	41.0
36	37.263337185760776	40.0	36.0	41.0	32.0	41.0
37	37.19065745362743	39.0	36.0	41.0	31.0	41.0
38	37.155365939623444	39.0	36.0	41.0	31.0	41.0
39	37.11082204244227	39.0	36.0	41.0	31.0	41.0
40	37.069633855460204	39.0	35.0	41.0	31.0	41.0
41	37.01784083737019	39.0	35.0	41.0	31.0	41.0
42	36.94726600601634	39.0	35.0	41.0	31.0	41.0
43	36.87803214727748	39.0	35.0	41.0	31.0	41.0
44	36.74160211801543	39.0	35.0	41.0	31.0	41.0
45	36.72476127244859	39.0	35.0	41.0	31.0	41.0
46	36.603434398078704	39.0	35.0	40.0	31.0	41.0
47	36.49820083441939	39.0	35.0	40.0	30.0	41.0
48	36.396439373447755	39.0	35.0	40.0	30.0	41.0
49	36.28049934016934	39.0	35.0	40.0	30.0	41.0
50	36.090659912623664	39.0	35.0	40.0	29.0	41.0
51	34.31113843329153	37.0	33.0	40.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	3.0
8	19.0
9	37.0
10	35.0
11	23.0
12	13.0
13	17.0
14	10.0
15	19.0
16	39.0
17	67.0
18	127.0
19	269.0
20	481.0
21	708.0
22	1118.0
23	1623.0
24	2258.0
25	3073.0
26	4061.0
27	4857.0
28	6003.0
29	7765.0
30	9780.0
31	12992.0
32	17242.0
33	24427.0
34	36562.0
35	48050.0
36	51661.0
37	79687.0
38	125402.0
39	171528.0
40	49.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	44.21816214621192	21.83490299259842	24.83078007557315	9.11615478561651
2	30.551061056876584	26.888795993475462	25.949459430660404	16.61068351898755
3	31.57810181883755	26.917812149080746	25.751592200063932	15.75249383201777
4	28.448127474364966	25.02028671896132	28.655502823747348	17.87608298292637
5	23.01817198219687	39.53606937648052	21.02540143113581	16.4203572101868
6	25.78650994663978	36.520520323603904	24.298653289727135	13.394316440029181
7	78.79787870591225	6.517159695412332	10.085163236366915	4.599798362308506
8	79.53393824640781	7.186170605158974	8.058130671060074	5.221760477373136
9	73.18366242899648	8.13058909353202	9.98352472520717	8.702223752264326
10	36.834452176621504	28.17878541979164	19.849017631003026	15.137744772583831
11	27.47370923189154	27.159777378873944	24.89094351685642	20.475569872378095
12	24.677994442668503	23.431119417053957	31.713838411160566	20.177047729116975
13	22.898172965795364	25.349464348652877	33.72005147498791	18.032311210563847
14	20.633437430840733	30.198113130220243	28.755829870246963	20.412619568692058
15	19.136072655142172	28.461078187883704	34.62758501979492	17.775264137179203
16	20.998516405603233	27.25239957049532	30.445816017901496	21.30326800599995
17	21.625724379308366	26.848960254424142	28.115835116105604	23.409480250161884
18	21.604413078581324	27.946328308784352	30.11286792731207	20.33639068532225
19	22.13309726969451	30.430078441979987	26.399619675248566	21.037204613076945
20	22.94800862288014	28.344357833132516	29.302054901189333	19.40557864279801
21	22.700305735198892	29.539266071589577	28.385341103761448	19.375087089450087
22	20.464586355849544	25.820444094720536	27.77419857214285	25.94077097728707
23	19.734592339407055	28.862550306964696	28.505831919410497	22.897025434217753
24	21.388513208908126	26.477323956361015	29.087466496176262	23.0466963385546
25	19.907541741461134	30.08155670855157	27.00682781288678	23.004073737100516
26	20.14622830960402	28.37976737895591	27.953541364415045	23.520462947025024
27	22.13686773059237	29.112220391636136	27.2364980614913	21.514413816280197
28	18.348538126736667	28.197473791198433	30.83712428586651	22.61686379619839
29	20.031311218760504	26.86781255891345	28.417144121769493	24.68373210055655
30	22.36850517618708	27.00109015499873	29.236153802018016	21.394250866796174
31	21.50884009147466	30.144998811485152	25.404873730543194	22.941287366496997
32	21.17458053622511	29.383857509364674	27.284530454668403	22.157031499741805
33	21.52949565987164	26.990926303882755	28.077966574044478	23.40161146220113
34	19.19017057237236	28.94976270686306	27.66157654445455	24.19849017631003
35	20.72196129539922	25.081925557987226	27.733707100761468	26.462406045852084
36	23.27685838640667	27.801739330005493	26.46027491577938	22.461127367808462
37	20.85441922607192	28.547962721617036	29.37844771764166	21.21917033466939
38	19.67737969360907	28.89795985278809	27.88337800509832	23.541282448504518
39	21.838673453496284	26.189293530380898	29.626642404570454	22.345390611552364
40	20.67868296161507	25.145203727838293	30.738600503274565	23.43751280727207
41	20.49343857837231	27.08026983385382	27.25289136974287	25.173400218031
42	22.0171965803559	26.189129597298383	27.875837083302596	23.91783673904312
43	23.91750887287809	25.339956229866967	27.567970754338077	23.17456414291686
44	20.815567085515692	26.08142556208556	28.888615667084693	24.214391685314055
45	20.57114285948476	25.02258178211654	28.389439430824336	26.016835927574366
46	23.32997270514176	26.084540290653358	27.487315677740348	23.098171326464538
47	19.572298587716492	26.097491004172095	31.077286251752035	23.252924156359374
48	20.67442070146966	25.917328546487322	28.57583134564471	24.83241940639831
49	21.181793591855804	24.373078909189267	31.25400611470398	23.19112138425095
50	19.997868869927295	23.824230948926647	31.384005049138942	24.793895132007115
51	19.493774641191468	23.69029762051131	28.075179711641706	28.74074802665552
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	104.0
1	241.5
2	379.0
3	780.0
4	1181.0
5	873.0
6	565.0
7	544.5
8	524.0
9	517.0
10	510.0
11	518.0
12	526.0
13	597.5
14	669.0
15	696.0
16	723.0
17	792.0
18	861.0
19	981.0
20	1101.0
21	1319.5
22	1538.0
23	1878.0
24	2218.0
25	3089.5
26	4746.0
27	5531.0
28	6545.0
29	7559.0
30	9145.0
31	10731.0
32	12918.0
33	15105.0
34	16933.5
35	18762.0
36	20447.5
37	22133.0
38	24153.5
39	26174.0
40	29928.5
41	33683.0
42	37198.5
43	40714.0
44	46919.5
45	53125.0
46	64827.0
47	76529.0
48	73664.0
49	70799.0
50	67928.5
51	65058.0
52	53382.5
53	41707.0
54	35979.5
55	30252.0
56	25867.5
57	21483.0
58	18843.0
59	16203.0
60	15478.5
61	14754.0
62	12770.5
63	10787.0
64	8406.0
65	6025.0
66	4718.0
67	3411.0
68	2747.5
69	2084.0
70	1469.0
71	854.0
72	818.5
73	783.0
74	611.0
75	374.0
76	309.0
77	196.5
78	84.0
79	58.5
80	33.0
81	23.0
82	13.0
83	13.5
84	14.0
85	9.5
86	5.0
87	3.0
88	1.0
89	0.5
90	0.0
91	0.5
92	1.0
93	0.5
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	610005.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	35.186215774925095
#Duplication Level	Percentage of deduplicated	Percentage of total
1	72.70296071846639	25.581420633158615
2	11.30762632472381	7.957451595279102
3	4.431099848040426	4.677409061201647
4	2.2290608304882205	3.13728861427969
5	1.487368283755968	2.6167430684508752
6	1.0197700825599407	2.1529110099460356
7	0.770215548046482	1.8970679326735993
8	0.5461115040856679	1.5372477775941757
9	0.47809595939806115	1.5140148829650006
>10	4.745055361653834	37.57748477646386
>50	0.23064572395635421	4.877977843836382
>100	0.043955025261033266	2.41603921020235
>500	0.005198981482487806	1.2108467434941703
>1k	0.002835808081356985	2.8460968504545026
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTC	4370	0.7163875705936837	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGC	4319	0.7080269833853822	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCG	3839	0.6293391037778379	No Hit
GCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	2473	0.4054065130613684	No Hit
CTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGCT	1086	0.17803132761206875	No Hit
CCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	1027	0.16835927574364143	No Hit
GAATGCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTT	960	0.1573757592150884	No Hit
GAATGACTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCT	954	0.1563921607199941	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTC	712	0.11672035475119057	No Hit
TCTGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTCTGC	674	0.11049089761559332	No Hit
GAATATGTCTCTTATACACATCTGACGCCGATTACGTCGTATGCCGTCTTC	620	0.1016385111597446	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	4.917992475471513E-4	0.0	0.0	0.21835886591093515	0.0
2	4.917992475471513E-4	0.0	0.0	1.1688428783370628	0.0
3	4.917992475471513E-4	0.0	0.0	1.5480200981959165	0.0
4	4.917992475471513E-4	0.0	0.0	2.113753165957656	0.0
5	4.917992475471513E-4	0.0	0.0	4.191441053761855	0.0
6	4.917992475471513E-4	0.0	0.0	5.267333874312506	0.0
7	4.917992475471513E-4	0.0	0.0	6.299292628748945	0.0
8	4.917992475471513E-4	0.0	0.0	7.844361931459578	0.0
9	4.917992475471513E-4	0.0	0.0	8.294850042212769	0.0
10	4.917992475471513E-4	0.0	0.0	10.191064007672068	0.0
11	4.917992475471513E-4	0.0	0.0	11.829247301251629	0.0
12	4.917992475471513E-4	0.0	0.0	13.894476274784633	0.0
13	4.917992475471513E-4	0.0	0.0	14.498733616937566	0.0
14	4.917992475471513E-4	0.0	0.0	14.7692232030885	0.0
15	4.917992475471513E-4	0.0	0.0	15.285284546847977	0.0
16	4.917992475471513E-4	0.0	0.0	16.217408054032344	0.0
17	4.917992475471513E-4	0.0	0.0	17.362644568487145	0.0
18	4.917992475471513E-4	0.0	0.0	18.601486873058416	0.0
19	4.917992475471513E-4	0.0	0.0	19.30344833239072	0.0
20	4.917992475471513E-4	0.0	0.0	19.986885353398744	0.0
21	4.917992475471513E-4	0.0	0.0	20.782288669765002	0.0
22	4.917992475471513E-4	0.0	0.0	21.653593003336038	0.0
23	4.917992475471513E-4	0.0	0.0	22.60276555110204	0.0
24	4.917992475471513E-4	0.0	0.0	23.293743493905787	0.0
25	4.917992475471513E-4	0.0	0.0	23.916853140548028	0.0
26	4.917992475471513E-4	0.0	0.0	24.478160015081844	0.0
27	6.557323300628683E-4	0.0	0.0	25.061106056507732	0.0
28	6.557323300628683E-4	0.0	0.0	25.661265071597775	0.0
29	6.557323300628683E-4	0.0	0.0	26.279784591929573	0.0
30	6.557323300628683E-4	0.0	0.0	26.972565798640996	0.0
31	6.557323300628683E-4	0.0	0.0	27.651248760256063	0.0
32	6.557323300628683E-4	0.0	0.0	28.272063343743085	0.0
33	6.557323300628683E-4	0.0	0.0	28.887632068589603	0.0
34	6.557323300628683E-4	0.0	0.0	29.486315685937	0.0
35	6.557323300628683E-4	0.0	0.0	30.100900812288423	0.0
36	6.557323300628683E-4	0.0	0.0	30.707453217596576	0.0
37	6.557323300628683E-4	0.0	0.0	31.29564511766297	0.0
38	6.557323300628683E-4	0.0	0.0	31.918590831222694	0.0
39	8.196654125785855E-4	0.0	0.0	32.53432348915173	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TACCCGC	45	3.8380676E-10	45.000004	28
TCCGCGG	20	7.03068E-4	45.000004	2
CTATCGT	20	7.03068E-4	45.000004	15
TTCGTAG	20	7.03068E-4	45.000004	1
CGACAGA	20	7.03068E-4	45.000004	37
ATACTCG	20	7.03068E-4	45.000004	14
TCTACCG	20	7.03068E-4	45.000004	3
TGCGAAG	40	6.8066583E-9	45.000004	1
GTCCGCG	20	7.03068E-4	45.000004	1
TACTCGA	20	7.03068E-4	45.000004	15
ACCGTCA	20	7.03068E-4	45.000004	41
ACGTACG	20	7.03068E-4	45.000004	1
CGGTCAA	20	7.03068E-4	45.000004	33
TAGTGCG	20	7.03068E-4	45.000004	1
TTACCCG	40	6.8066583E-9	45.000004	27
AGTACGA	20	7.03068E-4	45.000004	22
GTACGAA	20	7.03068E-4	45.000004	23
CTTACGG	40	6.8066583E-9	45.000004	2
AACACGT	45	3.8380676E-10	45.000004	41
GTCGATG	25	3.8886454E-5	45.0	1
>>END_MODULE