SRR2936769.fastq FastQC Report

Basic Statistics

Measure	Value
Filename	SRR2936769.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	643642
Sequences flagged as poor quality	0
Sequence length	51
%GC	48

Per base sequence quality

Per base quality graph

Per sequence quality scores

Per Sequence quality graph

Per base sequence content

Per sequence GC content

Per sequence GC content graph

Per base N content

N content graph

Sequence Length Distribution

Sequence length distribution

Sequence Duplication Levels

Duplication level graph

Overrepresented sequences

Sequence	Count	Percentage	Possible Source
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	12959	2.0133863234530995	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTC	11576	1.7985153237358658	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGC	10801	1.6781067736412476	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCG	9907	1.5392096848869403	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT	8117	1.2611047756361455	No Hit
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCGAC	5429	0.8434813141466841	No Hit
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCGACATCACTCGTATG	4822	0.7491742303951576	No Hit
GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCGACATCACTCGTA	4207	0.6536242196749125	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACCTGTCTCTTATACACATC	3083	0.4789929805699441	No Hit
GCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGC	2995	0.4653207839140392	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGCTGTCTCTTATACACATCTG	2720	0.4225951693643361	No Hit
GCTCAACCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTC	2372	0.36852784622507545	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCT	2309	0.3587397963464162	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTT	2217	0.34444613620615183	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1911	0.2969041796526641	No Hit
GAACTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCT	1887	0.29317539874650816	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTC	1862	0.2892912519692624	No Hit
GAATATGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTC	1567	0.24345831999776274	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCG	1488	0.2311844161816662	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGC	1485	0.23071831856839672	No Hit
CCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGC	1190	0.18488538659689702	No Hit
GCTCCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTC	1083	0.16826123839028528	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACATCACTCGTA	993	0.15427830999220063	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	961	0.1493066021173261	No Hit
GCTCAACTTGACCCTGCATCTGTCTCTTATACACATCTGACGCGACATCAC	945	0.14682074817988883	No Hit
CGCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTG	835	0.12973050236000758	No Hit
GCTCAACTCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGT	810	0.12584635558276186	No Hit
GCTCAACTTCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCG	782	0.12149611119224661	No Hit
CTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGCT	762	0.11838879377045004	TruSeq Adapter, Index 13 (95% over 22bp)
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	670	0.10409513363018573	No Hit

Adapter Content

Adapter graph

Kmer Content

Kmer graph

Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGATC	65	0.0	45.000004	6
AACCGTT	20	7.0309703E-4	45.0	37
TACCCGC	20	7.0309703E-4	45.0	28
TCGCAAA	20	7.0309703E-4	45.0	30
TTGTACG	20	7.0309703E-4	45.0	1
TCGATAG	20	7.0309703E-4	45.0	1
TAGCACG	25	3.888886E-5	45.0	1
CGGATCA	20	7.0309703E-4	45.0	31
TCGCTAA	20	7.0309703E-4	45.0	30
CGAATTT	20	7.0309703E-4	45.0	28
CGCACGA	20	7.0309703E-4	45.0	39
CCAACGG	25	3.888886E-5	45.0	2
CGCAAAT	20	7.0309703E-4	45.0	31
ATCGCAA	20	7.0309703E-4	45.0	29
ACCCAAT	20	7.0309703E-4	45.0	12
TCGCGCA	20	7.0309703E-4	45.0	34
AATGTCG	25	3.888886E-5	45.0	15
GTTGCCG	25	3.888886E-5	45.0	29
CGGTCTA	30	2.164008E-6	44.999996	31
CTCAACT	5120	0.0	44.16504	2

Summary

Basic Statistics

Per base sequence quality

Per sequence quality scores

Per base sequence content

Per sequence GC content

Per base N content

Sequence Length Distribution

Sequence Duplication Levels

Overrepresented sequences

Adapter Content

Kmer Content