##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936769.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	643642
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.865606967848585	31.0	31.0	34.0	30.0	34.0
2	31.95783214892751	33.0	31.0	34.0	30.0	34.0
3	31.913885358631042	33.0	31.0	34.0	30.0	34.0
4	35.634074532115676	37.0	35.0	37.0	33.0	37.0
5	30.94299470823843	37.0	35.0	37.0	0.0	37.0
6	33.22460001056488	37.0	35.0	37.0	17.0	37.0
7	35.072442444713054	37.0	35.0	37.0	32.0	37.0
8	35.44608804273183	37.0	35.0	37.0	33.0	37.0
9	37.506786381249206	39.0	37.0	39.0	35.0	39.0
10	37.30937073714891	39.0	37.0	39.0	34.0	39.0
11	37.09200456154198	39.0	37.0	39.0	33.0	39.0
12	37.03418049163976	39.0	37.0	39.0	33.0	39.0
13	36.82461057544411	39.0	35.0	39.0	33.0	39.0
14	38.04733842726236	40.0	37.0	41.0	33.0	41.0
15	38.02165023413637	40.0	37.0	41.0	33.0	41.0
16	38.061290282486226	40.0	37.0	41.0	33.0	41.0
17	38.023388778233866	40.0	37.0	41.0	33.0	41.0
18	37.861233418577406	40.0	36.0	41.0	33.0	41.0
19	37.83857175262025	40.0	37.0	41.0	33.0	41.0
20	37.877091613039546	40.0	36.0	41.0	33.0	41.0
21	37.84770105120548	40.0	36.0	41.0	33.0	41.0
22	37.9003794034572	40.0	36.0	41.0	33.0	41.0
23	37.90510252593833	40.0	36.0	41.0	33.0	41.0
24	37.82754699040771	40.0	36.0	41.0	33.0	41.0
25	37.72436230078211	39.0	36.0	41.0	33.0	41.0
26	37.65513282228319	40.0	36.0	41.0	33.0	41.0
27	37.63447382240438	40.0	36.0	41.0	33.0	41.0
28	37.45307639961345	39.0	36.0	41.0	33.0	41.0
29	37.37358811264647	39.0	36.0	41.0	32.0	41.0
30	37.28465358071723	39.0	36.0	41.0	32.0	41.0
31	37.23428551896862	39.0	35.0	41.0	32.0	41.0
32	37.115149415358225	39.0	35.0	41.0	31.0	41.0
33	36.969293489237806	39.0	35.0	41.0	31.0	41.0
34	36.842962392137245	39.0	35.0	41.0	31.0	41.0
35	36.71424487525674	39.0	35.0	41.0	31.0	41.0
36	36.52626149319031	39.0	35.0	40.0	30.0	41.0
37	36.41723349315303	39.0	35.0	40.0	30.0	41.0
38	36.30188210216238	39.0	35.0	40.0	30.0	41.0
39	36.17517968062992	39.0	35.0	40.0	30.0	41.0
40	35.96130457614637	38.0	35.0	40.0	29.0	41.0
41	36.003000114970746	38.0	35.0	40.0	29.0	41.0
42	35.99674042402454	38.0	35.0	40.0	29.0	41.0
43	35.95784147087978	38.0	35.0	40.0	29.0	41.0
44	35.825872146317366	38.0	35.0	40.0	29.0	41.0
45	35.7843071148247	38.0	35.0	40.0	28.0	41.0
46	35.60426137511225	38.0	34.0	40.0	28.0	41.0
47	35.425859406315936	38.0	34.0	40.0	28.0	41.0
48	35.37384601999248	38.0	34.0	40.0	28.0	41.0
49	35.30684138076757	37.0	34.0	40.0	27.0	41.0
50	35.08837676845203	37.0	34.0	40.0	26.0	41.0
51	33.233921962830266	35.0	31.0	39.0	22.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	17.0
9	40.0
10	36.0
11	28.0
12	19.0
13	16.0
14	11.0
15	22.0
16	46.0
17	96.0
18	220.0
19	413.0
20	642.0
21	1015.0
22	1545.0
23	2204.0
24	3249.0
25	4196.0
26	5609.0
27	6860.0
28	8088.0
29	9830.0
30	12603.0
31	16411.0
32	21678.0
33	30712.0
34	45100.0
35	55365.0
36	59774.0
37	88261.0
38	124414.0
39	145079.0
40	41.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	51.60275432616268	17.83538053762806	20.191814704447502	10.370050431761756
2	27.933385329111527	27.7534716503895	20.526317424903905	23.78682559559507
3	27.22212037126229	27.914430692838565	31.303581804792103	13.559867131107046
4	25.346854307208044	20.406064240680376	29.51500989680599	24.73207155530559
5	23.057382830828317	41.80553786110913	18.39376547832491	16.743313829737648
6	22.2984205505545	41.98374251524916	23.09063112724154	12.627205806954796
7	68.37418937856758	5.133443746679054	13.600883721074759	12.891483153678598
8	67.3366560914297	8.98201173944522	17.813784681546572	5.8675474875784985
9	62.29503357456474	5.740613570898108	19.30172362897387	12.662629225563279
10	46.20114908598258	20.942387227682467	18.612675990690477	14.243787695644471
11	28.189894382280833	29.055903747735538	23.546629958890193	19.207571911093435
12	25.412574070679046	18.324938397432113	27.952184599513398	28.310302932375453
13	22.842977928724352	19.361228757601275	32.569969020045306	25.225824293629067
14	20.40140326454768	24.953312555737504	26.499824436565667	28.145459743149143
15	17.97241323592929	21.57752290869769	41.44508904018072	19.004974815192295
16	26.38687344828336	22.752554991750074	28.608139307254653	22.252432252711912
17	21.688920238269098	21.71875048551835	26.79828227492923	29.794047001283325
18	20.926539908831305	30.3168531575006	28.874125678560443	19.882481255107653
19	20.800227455635277	26.803564714546287	29.724909188648347	22.67129864117009
20	22.639914735209945	25.62325019187685	33.84055111381793	17.896283959095275
21	22.80693304663151	33.72014256372331	26.208668794143332	17.264255595501847
22	27.184055732845273	22.393193731919297	25.955733156009085	24.46701737922634
23	20.701259395751055	32.50455998831649	27.30850379558823	19.485676820344228
24	22.88104256714136	29.74293162969477	24.426622252742984	22.949403550420886
25	20.93259917780381	33.365908377638505	22.724123037340632	22.977369407217054
26	20.48778668887363	23.90257316955699	30.345285111910037	25.264355029659345
27	22.846395977888328	24.466551281613068	28.045248756296203	24.641803984202397
28	23.19224040693429	23.760879495123067	28.68566687692848	24.361213221014168
29	23.617010698493885	27.881493128167524	23.95819415140715	24.543302021931446
30	20.061462738603137	31.104092026312763	27.541552602222975	21.292892632861125
31	26.57408932294661	22.914135497683493	23.00145111723598	27.51032406213392
32	24.19279661675279	32.520407307167645	21.313400927844985	21.97339514823458
33	19.474179745883582	24.002473424667752	25.582233601909138	30.941113227539535
34	23.175771624598767	23.43911677609603	27.707483352546912	25.67762824675829
35	23.54476556843711	30.074948496213732	23.287013588299086	23.093272347050068
36	22.785803288163294	24.72135131020039	25.622628728392492	26.870216673243824
37	20.135727624984074	31.22543277163392	24.442780303336324	24.19605930004568
38	18.700146976114052	30.610650019731466	23.06297600218755	27.62622700196693
39	21.955528073059245	25.774110452705074	24.655942278471574	27.614419195764107
40	20.79494501601822	23.860158286749467	33.56850547353964	21.776391223692674
41	18.795075523349936	26.43441540483685	26.123994394399368	28.646514677413844
42	19.751507825778926	23.42715360402211	28.398550747154474	28.422787823044487
43	21.70103877621411	23.597589964607653	26.24331538339636	28.45805587578188
44	22.43576398059791	22.48283983953813	28.316051469605775	26.765344710258187
45	21.486633874110144	24.627199592319954	25.774265818576165	28.111900714993737
46	24.78629424431594	24.33076151028056	25.065175982922185	25.817768262481316
47	20.523676205095377	21.136439200673664	34.031495769387334	24.308388824843625
48	19.643217813629317	24.638385935038425	27.484688693404095	28.233707557928167
49	21.50294729057457	22.202249076349897	29.538314777469466	26.756488855606065
50	19.424618033005924	22.349225190400876	33.14653176765966	25.079625008933537
51	20.615342069038377	21.6873665795582	26.09696073282974	31.60033061857368
>>END_MODULE
>>Per sequence GC content	fail
#GC Content	Count
0	159.0
1	389.5
2	620.0
3	1971.0
4	3322.0
5	2134.5
6	947.0
7	831.0
8	715.0
9	739.5
10	764.0
11	787.5
12	811.0
13	837.0
14	863.0
15	820.5
16	778.0
17	707.5
18	637.0
19	681.0
20	725.0
21	1064.0
22	1403.0
23	1594.0
24	1785.0
25	2134.0
26	3065.5
27	3648.0
28	4397.5
29	5147.0
30	12632.5
31	20118.0
32	14939.0
33	9760.0
34	11644.0
35	13528.0
36	14533.0
37	15538.0
38	20231.5
39	24925.0
40	26200.5
41	27476.0
42	27775.0
43	28074.0
44	34994.5
45	41915.0
46	70052.0
47	98189.0
48	80394.0
49	62599.0
50	60318.5
51	58038.0
52	47330.5
53	36623.0
54	33124.5
55	29626.0
56	28246.5
57	26867.0
58	25444.0
59	24021.0
60	23803.5
61	23586.0
62	22678.0
63	21770.0
64	20993.5
65	20217.0
66	17639.5
67	15062.0
68	12176.0
69	9290.0
70	7116.5
71	4943.0
72	3947.0
73	2951.0
74	2328.0
75	1340.5
76	976.0
77	793.5
78	611.0
79	433.5
80	256.0
81	172.0
82	88.0
83	67.0
84	46.0
85	32.0
86	18.0
87	14.0
88	10.0
89	6.5
90	3.0
91	3.0
92	3.0
93	2.5
94	2.0
95	1.0
96	0.0
97	0.5
98	1.0
99	0.5
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	643642.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	28.791516486761292
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.94651131859506	21.2903219976781
2	9.832031260405442	5.661581802646314
3	3.8434758816912358	3.319784976425478
4	2.1070987130793957	2.4266626934743565
5	1.3867739877073717	1.9963663065244246
6	0.9714522684720703	1.6781750402289162
7	0.7296887380944225	1.4706191733134726
8	0.5978016204574256	1.3769292168970069
9	0.47822373785780814	1.2391907969604323
>10	5.8745519584094055	37.617281970544234
>50	0.17224238187663482	3.068671584337229
>100	0.042103693347620895	2.4069833702509236
>500	0.006014813335374414	1.3359710170677834
>1k	0.008748819396908237	5.856168971587015
>5k	0.0016404036369202944	3.692261167015169
>10k+	0.0016404036369202944	5.563029915049163
>>END_MODULE
>>Overrepresented sequences	fail
#Sequence	Count	Percentage	Possible Source
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACAAAATAAAA	12959	2.0133863234530995	No Hit
GAATCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTC	11576	1.7985153237358658	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGC	10801	1.6781067736412476	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCG	9907	1.5392096848869403	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACTGTAAATACCTGTCTCTT	8117	1.2611047756361455	No Hit
GCTCAACTTGACCCTGCATTAGAACTGTCTCTTATACACATCTGACGCGAC	5429	0.8434813141466841	No Hit
GCTCAACTTGACCTGTCTCTTATACACATCTGACGCGACATCACTCGTATG	4822	0.7491742303951576	No Hit
GCTCAACTTGACCCCTGTCTCTTATACACATCTGACGCGACATCACTCGTA	4207	0.6536242196749125	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGACCTGTCTCTTATACACATC	3083	0.4789929805699441	No Hit
GCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGC	2995	0.4653207839140392	No Hit
GCTCAACTTGACCCTGCATTAGAAATTGAAGCTGTCTCTTATACACATCTG	2720	0.4225951693643361	No Hit
GCTCAACCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTC	2372	0.36852784622507545	No Hit
GAATGACTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCT	2309	0.3587397963464162	No Hit
GAATGCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTT	2217	0.34444613620615183	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	1911	0.2969041796526641	No Hit
GAACTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCT	1887	0.29317539874650816	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTC	1862	0.2892912519692624	No Hit
GAATATGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTC	1567	0.24345831999776274	No Hit
GAATAATACCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCG	1488	0.2311844161816662	No Hit
GAATAATACGGCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGC	1485	0.23071831856839672	No Hit
CCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGC	1190	0.18488538659689702	No Hit
GCTCCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTC	1083	0.16826123839028528	No Hit
GAATGATACGGCGACTGTCTCTTATACACATCTGACGCGACATCACTCGTA	993	0.15427830999220063	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	961	0.1493066021173261	No Hit
GCTCAACTTGACCCTGCATCTGTCTCTTATACACATCTGACGCGACATCAC	945	0.14682074817988883	No Hit
CGCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTG	835	0.12973050236000758	No Hit
GCTCAACTCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGT	810	0.12584635558276186	No Hit
GCTCAACTTCTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCG	782	0.12149611119224661	No Hit
CTGTCTCTTATACACATCTGACGCGACATCACTCGTATGCCGTCTTCTGCT	762	0.11838879377045004	TruSeq Adapter, Index 13 (95% over 22bp)
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	670	0.10409513363018573	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	1.5536587108982945E-4	0.0	0.0	0.1471314799220685	0.0
2	1.5536587108982945E-4	0.0	0.0	1.0771515842657875	0.0
3	1.5536587108982945E-4	0.0	0.0	1.4818796784547932	0.0
4	1.5536587108982945E-4	0.0	0.0	2.1547692661448443	0.0
5	1.5536587108982945E-4	0.0	0.0	5.287255959057986	0.0
6	1.5536587108982945E-4	0.0	0.0	6.5213270731245006	0.0
7	1.5536587108982945E-4	0.0	0.0	7.659692810599681	0.0
8	1.5536587108982945E-4	0.0	0.0	9.431019106894826	0.0
9	1.5536587108982945E-4	0.0	0.0	9.977130143775577	0.0
10	1.5536587108982945E-4	0.0	0.0	12.765792163966927	0.0
11	1.5536587108982945E-4	0.0	0.0	13.918917659195639	0.0
12	1.5536587108982945E-4	0.0	0.0	16.857818476730852	0.0
13	1.5536587108982945E-4	0.0	0.0	18.170193989826643	0.0
14	1.5536587108982945E-4	0.0	0.0	18.424838652542874	0.0
15	1.5536587108982945E-4	0.0	0.0	19.605774638696666	0.0
16	1.5536587108982945E-4	0.0	0.0	20.219625195372583	0.0
17	1.5536587108982945E-4	0.0	0.0	20.885212587121412	0.0
18	1.5536587108982945E-4	0.0	0.0	21.63329925641894	0.0
19	1.5536587108982945E-4	0.0	0.0	22.11850687183248	0.0
20	1.5536587108982945E-4	0.0	0.0	22.688544252861064	0.0
21	1.5536587108982945E-4	0.0	0.0	23.222536751796806	0.0
22	1.5536587108982945E-4	0.0	0.0	23.750936079373318	0.0
23	1.5536587108982945E-4	0.0	0.0	24.39415078568521	0.0
24	1.5536587108982945E-4	0.0	0.0	24.85745181327508	0.0
25	1.5536587108982945E-4	0.0	0.0	26.13487000537566	0.0
26	1.5536587108982945E-4	0.0	0.0	26.566942492876475	0.0
27	1.5536587108982945E-4	0.0	0.0	27.020455470587688	0.0
28	1.5536587108982945E-4	0.0	0.0	27.39053697552366	0.0
29	1.5536587108982945E-4	0.0	0.0	27.808005071142034	0.0
30	1.5536587108982945E-4	0.0	0.0	28.288862442165055	0.0
31	1.5536587108982945E-4	0.0	0.0	28.72295468599004	0.0
32	1.5536587108982945E-4	0.0	0.0	29.542509655988887	0.0
33	1.5536587108982945E-4	0.0	0.0	29.935896041588336	0.0
34	1.5536587108982945E-4	0.0	0.0	30.858458584119745	0.0
35	1.5536587108982945E-4	0.0	0.0	31.30870887853807	0.0
36	1.5536587108982945E-4	0.0	0.0	31.697123556262643	0.0
37	1.5536587108982945E-4	0.0	0.0	32.115834578849736	0.0
38	1.5536587108982945E-4	0.0	0.0	32.54666413938183	0.0
39	1.5536587108982945E-4	0.0	0.0	33.14606567004639	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CGGGATC	65	0.0	45.000004	6
AACCGTT	20	7.0309703E-4	45.0	37
TACCCGC	20	7.0309703E-4	45.0	28
TCGCAAA	20	7.0309703E-4	45.0	30
TTGTACG	20	7.0309703E-4	45.0	1
TCGATAG	20	7.0309703E-4	45.0	1
TAGCACG	25	3.888886E-5	45.0	1
CGGATCA	20	7.0309703E-4	45.0	31
TCGCTAA	20	7.0309703E-4	45.0	30
CGAATTT	20	7.0309703E-4	45.0	28
CGCACGA	20	7.0309703E-4	45.0	39
CCAACGG	25	3.888886E-5	45.0	2
CGCAAAT	20	7.0309703E-4	45.0	31
ATCGCAA	20	7.0309703E-4	45.0	29
ACCCAAT	20	7.0309703E-4	45.0	12
TCGCGCA	20	7.0309703E-4	45.0	34
AATGTCG	25	3.888886E-5	45.0	15
GTTGCCG	25	3.888886E-5	45.0	29
CGGTCTA	30	2.164008E-6	44.999996	31
CTCAACT	5120	0.0	44.16504	2
>>END_MODULE