##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936768.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	304911
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.97131294049739	33.0	31.0	34.0	30.0	34.0
2	32.059879768194655	33.0	31.0	34.0	30.0	34.0
3	31.996113620039946	33.0	31.0	34.0	30.0	34.0
4	35.7034839674528	37.0	35.0	37.0	33.0	37.0
5	30.7274122612828	37.0	35.0	37.0	0.0	37.0
6	33.152824922682356	37.0	35.0	37.0	17.0	37.0
7	35.127715956459426	37.0	35.0	37.0	32.0	37.0
8	35.5435684511218	37.0	35.0	37.0	33.0	37.0
9	37.50664948132406	39.0	37.0	39.0	35.0	39.0
10	37.33948266871317	39.0	37.0	39.0	34.0	39.0
11	37.190435897688175	39.0	37.0	39.0	34.0	39.0
12	37.05177248442988	39.0	37.0	39.0	33.0	39.0
13	36.86255005558999	39.0	35.0	39.0	33.0	39.0
14	38.01898258836185	40.0	37.0	41.0	33.0	41.0
15	38.120241644283084	40.0	37.0	41.0	33.0	41.0
16	38.105020809350926	40.0	37.0	41.0	33.0	41.0
17	38.08289304092014	40.0	37.0	41.0	33.0	41.0
18	38.02247869050313	40.0	36.0	41.0	33.0	41.0
19	37.9876980495948	40.0	36.0	41.0	34.0	41.0
20	37.939546293836564	40.0	36.0	41.0	33.0	41.0
21	37.87539970679969	40.0	36.0	41.0	33.0	41.0
22	37.86779092915638	40.0	36.0	41.0	33.0	41.0
23	37.91082971752413	40.0	36.0	41.0	34.0	41.0
24	37.89842281846178	40.0	36.0	41.0	34.0	41.0
25	37.7228732318611	40.0	36.0	41.0	33.0	41.0
26	37.6569064415518	40.0	36.0	41.0	33.0	41.0
27	37.59242205102473	40.0	36.0	41.0	33.0	41.0
28	37.43499906530102	39.0	36.0	41.0	33.0	41.0
29	37.30468890922269	39.0	36.0	41.0	32.0	41.0
30	37.29373161348722	39.0	36.0	41.0	32.0	41.0
31	37.169980748480704	39.0	35.0	41.0	32.0	41.0
32	36.98797026017428	39.0	35.0	41.0	31.0	41.0
33	36.948991672979986	39.0	35.0	41.0	31.0	41.0
34	36.91701512900486	39.0	35.0	41.0	31.0	41.0
35	36.772792716563195	39.0	35.0	41.0	31.0	41.0
36	36.68791876973937	39.0	35.0	41.0	31.0	41.0
37	36.63253867521998	39.0	35.0	41.0	30.0	41.0
38	36.48339023518338	39.0	35.0	41.0	30.0	41.0
39	36.34974467959503	39.0	35.0	40.0	30.0	41.0
40	36.238738517141066	39.0	35.0	40.0	30.0	41.0
41	36.22612500040996	39.0	35.0	40.0	30.0	41.0
42	36.19791348951006	39.0	35.0	40.0	30.0	41.0
43	36.039483652606826	38.0	35.0	40.0	30.0	41.0
44	35.88049299631696	38.0	35.0	40.0	29.0	41.0
45	35.75404626267993	38.0	35.0	40.0	28.0	41.0
46	35.53391317466408	38.0	35.0	40.0	27.0	41.0
47	35.428305308762226	38.0	34.0	40.0	27.0	41.0
48	35.24438278710837	38.0	34.0	40.0	26.0	41.0
49	35.1144530699122	38.0	34.0	40.0	26.0	41.0
50	34.89288021750609	38.0	34.0	40.0	25.0	41.0
51	32.91562455929763	35.0	31.0	39.0	19.0	40.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	17.0
9	38.0
10	18.0
11	13.0
12	10.0
13	9.0
14	8.0
15	11.0
16	15.0
17	43.0
18	84.0
19	185.0
20	332.0
21	593.0
22	816.0
23	1121.0
24	1601.0
25	2047.0
26	2525.0
27	3163.0
28	3817.0
29	4600.0
30	5692.0
31	7616.0
32	9771.0
33	14017.0
34	21634.0
35	28055.0
36	26545.0
37	39216.0
38	58164.0
39	73099.0
40	34.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	45.46277438334465	20.56304954560511	23.485869647208528	10.488306423841712
2	33.94662704854859	25.810154438508288	22.895533450744644	17.34768506219848
3	32.559992915965644	25.702582064930425	25.274260357940513	16.46316466116342
4	29.376768958810935	24.68818770067331	25.85541354690385	20.079629793611904
5	21.662386729242304	38.851336947502716	20.269849234694714	19.216427088560266
6	25.781621522345866	35.22568880755368	24.826260777735143	14.166428892365312
7	76.42328417144675	7.223091328289239	11.077986691198415	5.275637809065596
8	74.61423169383853	7.65272489349351	10.827749736808446	6.905293675859513
9	66.7457061240821	9.849103508892759	13.591179065366616	9.814011301658516
10	38.55452902650281	25.971185034321493	19.914335658602017	15.559950280573675
11	29.582730698466108	25.864924518957995	24.579631433434674	19.972713349141223
12	29.229512874248552	21.134363797960717	30.896228735598257	18.739894592192478
13	24.798055826126312	24.38482048860159	31.539695189743895	19.277428495528206
14	18.532620994322933	32.08969174611608	28.880886553781266	20.49680070577972
15	18.598213905041145	27.305344838329876	35.872762871788815	18.223678384840166
16	20.054704487538988	26.10040306843636	32.29729330853922	21.547599135485438
17	19.84939867699099	24.060463545100045	28.39385919169856	27.696278586210404
18	22.41539334428735	25.97544857351817	29.777541643299195	21.831616438895285
19	23.333366129788693	28.772986215649816	26.832419952051584	21.061227702509914
20	24.64063284040261	26.41328125256222	28.11705710846772	20.82902879856745
21	23.685272095791888	27.803850959788267	29.030766354772375	19.48011058964747
22	19.753305062788815	25.81802558779447	27.52934462843256	26.899324720984158
23	20.645368648556463	27.081345048227192	27.941596072296505	24.331690230919843
24	22.594461990548062	24.648831954242386	28.617203052694062	24.13950300251549
25	20.3875885094339	27.384056331191726	26.16271633361866	26.06563882575571
26	18.649704339954937	28.2607055829406	27.847142280862286	25.242447796242185
27	21.807675026483135	27.154809108231582	27.985543322477707	23.051972542807572
28	19.316784241959127	27.971768811226884	30.181921937876954	22.529525008937036
29	20.916923298929852	27.885186169078846	29.30724047344963	21.890650058541674
30	24.362846863510992	25.80425107654365	28.83464355172493	20.99825850822043
31	26.10827421772255	27.99210261354953	25.03812587935496	20.861497289372966
32	24.319883506990564	30.779473354519844	25.218834348383627	19.681808790105965
33	23.91419135419844	28.012436415872173	24.67178947299376	23.40158275693563
34	22.841419299402123	27.436202695212703	27.210235117788468	22.51214288759671
35	21.452489414944033	28.84054691368957	26.043993165218705	23.662970506147694
36	24.617347357097643	28.690339148144865	25.250318945528367	21.44199454922912
37	24.597013554775	27.02887071965262	27.680864252191622	20.69325147338076
38	22.129408253555955	29.737529967761084	25.170623559005744	22.962438219677217
39	23.724627842222812	24.401218716281146	26.94392790027254	24.930225541223507
40	23.15429748352798	25.98331972280436	29.05700351905966	21.805379274608
41	20.40300284345268	28.173139047131784	26.128935984598783	25.29492212481675
42	23.177582966832944	26.28143950201862	26.68221218650688	23.85876534464155
43	24.17721892617846	23.0437734289678	27.230568920111114	25.548438724742628
44	22.662022688587818	24.470091272535264	27.381104650209405	25.486781388667513
45	23.51112291783504	25.104702683733944	25.96495370780326	25.419220690627757
46	24.82822856505669	27.06691460786918	25.88919389592373	22.2156629311504
47	22.132359934538275	25.02303950988977	29.079305108703853	23.765295446868105
48	20.93496134937736	25.19226921954275	28.914994867354736	24.957774563725152
49	21.548583029146208	23.112645985221917	30.701417790765174	24.637353194866698
50	19.969105739051724	22.68432427823201	31.13925046980922	26.207319512907045
51	19.51454686777453	22.870280180118133	28.707393304931607	28.907779647175737
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	248.0
1	334.5
2	421.0
3	649.0
4	877.0
5	628.5
6	380.0
7	353.0
8	326.0
9	353.5
10	381.0
11	400.5
12	420.0
13	419.0
14	418.0
15	404.0
16	390.0
17	459.0
18	528.0
19	542.5
20	557.0
21	826.5
22	1096.0
23	1255.5
24	1415.0
25	1672.5
26	2192.5
27	2455.0
28	3107.5
29	3760.0
30	4309.5
31	4859.0
32	5324.5
33	5790.0
34	6603.5
35	7417.0
36	7695.5
37	7974.0
38	8996.0
39	10018.0
40	11701.5
41	13385.0
42	15385.0
43	17385.0
44	20450.0
45	23515.0
46	29638.5
47	35762.0
48	35767.0
49	35772.0
50	34454.5
51	33137.0
52	28038.0
53	22939.0
54	19559.0
55	16179.0
56	14127.0
57	12075.0
58	11602.0
59	11129.0
60	10623.5
61	10118.0
62	9028.5
63	7939.0
64	6334.5
65	4730.0
66	3952.5
67	3175.0
68	2655.5
69	2136.0
70	1850.5
71	1565.0
72	1225.5
73	886.0
74	804.5
75	576.5
76	430.0
77	299.0
78	168.0
79	109.0
80	50.0
81	36.5
82	23.0
83	20.0
84	17.0
85	10.5
86	4.0
87	4.5
88	5.0
89	3.5
90	2.0
91	1.5
92	1.0
93	1.0
94	1.0
95	0.5
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	304911.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.30909103191404
#Duplication Level	Percentage of deduplicated	Percentage of total
1	70.14948560055743	24.06765087311468
2	12.330069894568508	8.460669808852275
3	5.03454871746495	5.181923707563331
4	2.635284900163542	3.616569181389578
5	1.6430444542971983	2.818568087598205
6	1.0846508595281124	2.2328031046436285
7	0.796531754874388	1.9129796341456933
8	0.6031300419721835	1.6554274811284624
9	0.4466151890729072	1.3790665060324088
>10	4.997504107301893	38.33202736167349
>50	0.21632922215414843	4.637589753557235
>100	0.053833078324073795	2.8966551827477116
>500	0.0049845442892660925	1.176584464358499
>1k	0.003987635431412874	1.6314848531947792
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTC	1426	0.4676774534208342	No Hit
GAATGATACGGCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGC	1187	0.3892939251125738	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCG	1133	0.3715838392186573	No Hit
GCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC	1024	0.335835702877233	TruSeq Adapter, Index 15 (95% over 22bp)
GAACTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCT	772	0.253188635372289	No Hit
CCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC	743	0.24367766331814858	TruSeq Adapter, Index 15 (95% over 22bp)
CTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGCT	731	0.239742088675056	Illumina Single End Adapter 1 (95% over 21bp)
TGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	666	0.21842439269163788	No Hit
GGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	528	0.17316528429607328	No Hit
CGTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTTT	381	0.12495449491818923	No Hit
TCTGTCTCTTATACACATCTGACGCAGGTAGACTCGTATGCCGTCTTCTGC	327	0.10724440902427265	TruSeq Adapter, Index 15 (95% over 22bp)
AGGGCAGGGACTTAATCAACGCAAGCTTATGACCCGCACTTACTGGGAATT	308	0.10101308250604274	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.32763658903745685	0.0
2	0.0	0.0	0.0	1.4148390841917806	0.0
3	0.0	0.0	0.0	1.855623444218149	0.0
4	0.0	0.0	0.0	2.7653971158797157	0.0
5	0.0	0.0	0.0	5.033927933068994	0.0
6	0.0	0.0	0.0	6.28478474046525	0.0
7	0.0	0.0	0.0	7.467424920714569	0.0
8	0.0	0.0	0.0	9.129221313760409	0.0
9	0.0	0.0	0.0	9.731364234153572	0.0
10	0.0	0.0	0.0	11.496797426134183	0.0
11	0.0	0.0	0.0	13.569533404829606	0.0
12	0.0	0.0	0.0	15.721636805494063	0.0
13	0.0	0.0	0.0	16.727176126804213	0.0
14	0.0	0.0	0.0	17.09810403691569	0.0
15	0.0	0.0	0.0	17.70254270918399	0.0
16	0.0	0.0	0.0	18.964878275955936	0.0
17	0.0	0.0	0.0	20.166540400313533	0.0
18	0.0	0.0	0.0	21.761104059873208	0.0
19	3.279645535910479E-4	0.0	0.0	22.58167137295801	0.0
20	3.279645535910479E-4	0.0	0.0	23.42978770854446	0.0
21	3.279645535910479E-4	0.0	0.0	24.354647749671216	0.0
22	3.279645535910479E-4	0.0	0.0	25.32673468651508	0.0
23	3.279645535910479E-4	0.0	0.0	26.200760221835225	0.0
24	3.279645535910479E-4	0.0	0.0	26.91047551580625	0.0
25	3.279645535910479E-4	0.0	0.0	27.547054714326475	0.0
26	3.279645535910479E-4	0.0	0.0	28.157396748559417	0.0
27	3.279645535910479E-4	0.0	0.0	28.791680195204503	0.0
28	3.279645535910479E-4	0.0	0.0	29.415468776134674	3.279645535910479E-4
29	3.279645535910479E-4	0.0	0.0	30.034337888760984	3.279645535910479E-4
30	3.279645535910479E-4	0.0	0.0	30.69420257058617	3.279645535910479E-4
31	3.279645535910479E-4	0.0	0.0	31.34554017401799	3.279645535910479E-4
32	3.279645535910479E-4	0.0	0.0	31.977855833341533	3.279645535910479E-4
33	3.279645535910479E-4	0.0	0.0	32.61410706730817	3.279645535910479E-4
34	3.279645535910479E-4	0.0	0.0	33.201491582789735	3.279645535910479E-4
35	3.279645535910479E-4	0.0	0.0	33.98565483042593	3.279645535910479E-4
36	3.279645535910479E-4	0.0	0.0	34.632728894661064	3.279645535910479E-4
37	6.559291071820957E-4	0.0	0.0	35.21289818996363	3.279645535910479E-4
38	6.559291071820957E-4	0.0	0.0	35.81372925214243	3.279645535910479E-4
39	6.559291071820957E-4	0.0	0.0	36.40734509414222	3.279645535910479E-4
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TCACGGA	25	3.8840415E-5	45.000004	14
CCTTATC	25	3.8840415E-5	45.000004	43
AGTCCAT	25	3.8840415E-5	45.000004	8
GACGGGA	115	0.0	45.000004	4
TTAGTCG	25	3.8840415E-5	45.000004	1
CGTTTAC	25	3.8840415E-5	45.000004	33
TCTATGT	25	3.8840415E-5	45.000004	32
AAGTAAG	20	7.025125E-4	45.0	1
CGAGTCG	20	7.025125E-4	45.0	35
GGCGTCA	20	7.025125E-4	45.0	36
GCTATCG	20	7.025125E-4	45.0	1
TAAGTAC	40	6.7866495E-9	45.0	9
TGTTGCG	40	6.7866495E-9	45.0	1
CTACGAA	35	1.208009E-7	45.0	11
TATTCGA	20	7.025125E-4	45.0	32
ACGTGAG	20	7.025125E-4	45.0	1
CTGCACG	20	7.025125E-4	45.0	1
CATACGT	20	7.025125E-4	45.0	10
CTCGCGG	20	7.025125E-4	45.0	2
GCGATAT	20	7.025125E-4	45.0	9
>>END_MODULE