##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936767.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	174831
Sequences flagged as poor quality	0
Sequence length	51
%GC	47
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	31.995961814552338	33.0	31.0	34.0	30.0	34.0
2	32.08708981816726	33.0	31.0	34.0	30.0	34.0
3	32.03466776487007	33.0	31.0	34.0	30.0	34.0
4	35.7137635773976	37.0	35.0	37.0	33.0	37.0
5	30.957364540613508	37.0	35.0	37.0	0.0	37.0
6	33.28296469161648	37.0	35.0	37.0	17.0	37.0
7	35.20201222895253	37.0	35.0	37.0	32.0	37.0
8	35.62623905371473	37.0	35.0	37.0	33.0	37.0
9	37.61013779020883	39.0	37.0	39.0	35.0	39.0
10	37.337148446213774	39.0	37.0	39.0	34.0	39.0
11	37.159496885563776	39.0	37.0	39.0	34.0	39.0
12	36.89686039661159	39.0	35.0	39.0	33.0	39.0
13	36.57488660477833	39.0	35.0	39.0	33.0	39.0
14	37.7315865035377	40.0	36.0	41.0	33.0	41.0
15	37.8773215276467	40.0	36.0	41.0	33.0	41.0
16	37.912704268693766	40.0	36.0	41.0	33.0	41.0
17	37.89803867735127	40.0	36.0	41.0	33.0	41.0
18	37.84173859327007	40.0	36.0	41.0	33.0	41.0
19	37.81472393339854	40.0	36.0	41.0	34.0	41.0
20	37.77380441683683	39.0	36.0	41.0	33.0	41.0
21	37.726106926117225	39.0	35.0	41.0	33.0	41.0
22	37.74541128289605	39.0	35.0	41.0	33.0	41.0
23	37.74507953394993	39.0	35.0	41.0	34.0	41.0
24	37.7280001830339	39.0	35.0	41.0	34.0	41.0
25	37.57279887434151	39.0	35.0	41.0	33.0	41.0
26	37.521669497972326	39.0	35.0	41.0	33.0	41.0
27	37.46993382180506	39.0	35.0	41.0	33.0	41.0
28	37.4086632233414	39.0	35.0	41.0	33.0	41.0
29	37.3857496668211	39.0	35.0	41.0	33.0	41.0
30	37.32867740846875	39.0	35.0	41.0	33.0	41.0
31	37.27184538211187	39.0	35.0	41.0	33.0	41.0
32	37.17221774170485	39.0	35.0	41.0	33.0	41.0
33	37.073659705658606	39.0	35.0	41.0	32.0	41.0
34	37.03163626587962	39.0	35.0	41.0	32.0	41.0
35	37.00820220670247	39.0	35.0	41.0	32.0	41.0
36	36.86032797387191	39.0	35.0	41.0	32.0	41.0
37	36.80290108733577	39.0	35.0	41.0	31.0	41.0
38	36.73088868678896	39.0	35.0	41.0	31.0	41.0
39	36.70362235530312	39.0	35.0	41.0	31.0	41.0
40	36.55464419925528	39.0	35.0	40.0	31.0	41.0
41	36.503766494500404	39.0	35.0	40.0	31.0	41.0
42	36.46266394403739	38.0	35.0	40.0	31.0	41.0
43	36.31190120745177	38.0	35.0	40.0	30.0	41.0
44	36.154223221282265	38.0	35.0	40.0	30.0	41.0
45	36.1373440636958	38.0	35.0	40.0	30.0	41.0
46	35.93255772717653	38.0	35.0	40.0	30.0	41.0
47	35.85343560352569	38.0	35.0	40.0	30.0	41.0
48	35.70627634687212	38.0	35.0	40.0	29.0	41.0
49	35.53591182341805	38.0	35.0	40.0	29.0	41.0
50	35.35015529282564	37.0	34.0	40.0	28.0	41.0
51	33.523682870886745	35.0	32.0	39.0	23.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
8	5.0
9	17.0
10	12.0
11	9.0
12	7.0
13	3.0
14	2.0
15	6.0
16	10.0
17	15.0
18	72.0
19	78.0
20	168.0
21	253.0
22	405.0
23	541.0
24	728.0
25	952.0
26	1110.0
27	1537.0
28	1883.0
29	2286.0
30	2997.0
31	4116.0
32	5388.0
33	8488.0
34	14199.0
35	18944.0
36	14375.0
37	21335.0
38	31988.0
39	42890.0
40	12.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	43.880661896345615	21.332601197728092	23.952845891174906	10.833891014751389
2	30.87209934164994	26.298539732656106	24.688985363007703	18.140375562686252
3	31.42577689311392	26.046868118354293	25.99939370020191	16.527961288329873
4	27.327533446585562	24.780502313662907	28.04365358546253	19.848310654289
5	23.234437828531554	38.884980352454654	20.76119223707466	17.119389581939128
6	26.153256573490975	34.531061425033315	24.27201125658493	15.04367074489078
7	78.34480155121231	6.286070548129337	10.343703347804452	5.025424552853899
8	78.01591250979517	6.549181781263049	9.388495175340758	6.04641053360102
9	71.3775017016433	7.212107692571683	11.620936790386144	9.789453815398872
10	42.44270180917572	22.736814409343882	19.384434110655434	15.43604967082497
11	33.36650822794584	23.89850770172338	23.613661192809055	19.12132287752172
12	31.25418261063541	19.931247890820277	29.19905508748448	19.615514411059824
13	22.381042263671773	27.89894240723899	31.72263500180174	17.997380327287495
14	17.444274756765104	31.703759630729106	29.042332309487445	21.809633303018344
15	16.392973786113448	25.603010907676556	39.9717441414852	18.032271164724794
16	16.756181684026288	25.478891043350437	35.23231005942882	22.532617213194456
17	17.41510372874376	24.639223021088938	29.172171983229518	28.773501266937785
18	22.243194856747373	25.487470757474362	30.42195034061465	21.847384045163615
19	22.746538085350995	27.31494986587047	27.087301451115646	22.851210597662885
20	25.254674514245185	25.839811017496896	28.02649415721468	20.879020311043238
21	22.35644708318319	27.87720713145838	28.946811492241082	20.819534293117353
22	20.01704503205953	25.665356830310415	27.35270060801574	26.964897529614312
23	18.806733359644458	28.29132133317318	28.733462601026133	24.16848270615623
24	22.428516681824163	23.375717121105524	29.726993496576693	24.46877270049362
25	18.853063815913654	27.569481384880255	29.08122701351591	24.49622778569018
26	17.59070187781343	28.791804657068827	28.21067202040828	25.40682144470946
27	21.123256173104313	28.319348399311334	28.32907207531845	22.228323352265903
28	16.793360445229965	27.201697639434652	33.31560192414389	22.689339991191492
29	18.518454965080565	23.865332807110867	33.13371198471667	24.482500243091902
30	23.10116626913991	25.91531250178744	29.63890843157106	21.344612797501586
31	24.195365810411197	25.55896837517374	27.07414588945896	23.1715199249561
32	24.47792439555914	26.042292270821537	27.401318988051322	22.078464345568005
33	24.59975633611888	26.816182484799604	24.995567147702637	23.588494031378872
34	19.09615571609154	23.538159708518513	30.379051769995023	26.986632805394922
35	20.125721410962587	23.632536563881693	29.62060504144002	26.621136983715704
36	25.539521023159505	23.732061247719226	30.280099067099087	20.44831866202218
37	21.539086317643896	25.471455291109702	30.677625821507625	22.311832569738776
38	18.972607832707013	28.192940611218837	27.91667381642844	24.917777739645715
39	23.419187672666748	23.82014631272486	27.130772002676874	25.62989401193152
40	22.199152324244555	23.990596633320177	30.293254628755772	23.516996413679497
41	19.611510544468658	23.353409864383316	28.771785324113004	28.263294267035022
42	22.006966727868626	21.853103854579565	31.08888011851445	25.051049299037352
43	25.20033632479366	23.00049762341919	27.36642815061402	24.432737901173134
44	21.545950088943037	24.40757073974295	28.35652716051501	25.689952010799
45	20.085682745050935	24.94980867237504	26.487865424324063	28.476643158249964
46	23.268756685027256	26.117221774170485	27.631255326572518	22.98276621422974
47	19.93353581458666	22.67103660106045	33.80750553391561	23.587922050437278
48	21.712968523888783	21.2971383793492	30.80803747619129	26.181855620570722
49	20.267572684478154	20.87387248256888	33.72456829738433	25.133986535568635
50	20.153176496159148	22.518889670596177	32.01320132013201	25.314732513112663
51	19.153925791192638	22.75225789476695	27.84860808437863	30.245208229661785
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	68.0
1	108.5
2	149.0
3	246.5
4	344.0
5	264.5
6	185.0
7	186.0
8	187.0
9	175.0
10	163.0
11	160.0
12	157.0
13	169.5
14	182.0
15	217.0
16	252.0
17	273.5
18	295.0
19	284.0
20	273.0
21	386.0
22	499.0
23	480.0
24	461.0
25	642.5
26	1020.0
27	1216.0
28	1621.5
29	2027.0
30	2092.5
31	2158.0
32	2454.0
33	2750.0
34	3338.5
35	3927.0
36	4559.0
37	5191.0
38	5401.5
39	5612.0
40	6961.5
41	8311.0
42	10101.0
43	11891.0
44	13622.0
45	15353.0
46	18901.0
47	22449.0
48	21450.0
49	20451.0
50	19673.0
51	18895.0
52	16017.5
53	13140.0
54	11139.5
55	9139.0
56	7837.5
57	6536.0
58	5966.5
59	5397.0
60	5265.5
61	5134.0
62	4666.0
63	4198.0
64	3380.5
65	2563.0
66	2246.5
67	1930.0
68	1423.5
69	917.0
70	844.5
71	772.0
72	543.5
73	315.0
74	271.0
75	179.0
76	131.0
77	112.0
78	93.0
79	59.0
80	25.0
81	23.5
82	22.0
83	16.5
84	11.0
85	10.0
86	9.0
87	5.5
88	2.0
89	1.0
90	0.0
91	0.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	174831.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	34.9245843128507
#Duplication Level	Percentage of deduplicated	Percentage of total
1	73.65662719664586	25.724270867294702
2	10.52260927954929	7.349955099496085
3	4.079660656086736	4.274413576539629
4	2.271573396223325	3.173350263969205
5	1.3691675264907712	2.390880335867209
6	1.0088602826774105	2.1140415601352163
7	0.6976858448385987	1.7056471678363678
8	0.5175322229319183	1.4459678203522257
9	0.38323588660148383	1.204591862999125
>10	5.319445126844528	42.78531839319114
>50	0.13102081593213122	2.8478931082016348
>100	0.03603072438133609	2.499556714770264
>500	0.00163776019915164	0.5016272857788379
>1k	0.0049132805974549205	1.9824859435683604
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGC	1213	0.6938128821547666	No Hit
GAATCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTC	1192	0.6818012823812711	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCG	1061	0.6068717790323227	No Hit
GCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC	877	0.5016272857788379	TruSeq Adapter, Index 16 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC	485	0.2774107566735876	TruSeq Adapter, Index 16 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGCT	371	0.21220492933175464	TruSeq Adapter, Index 13 (95% over 23bp)
GAATGCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTT	334	0.1910416344927387	No Hit
GACCAGAAAAATGGTCCTGCCAAGCGGCTACAGTGTTCTTCTTTCAGATAC	320	0.18303390131040834	No Hit
GAATGACTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCT	257	0.1469991019899217	No Hit
TCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTCTGC	215	0.12297590244293059	TruSeq Adapter, Index 16 (95% over 21bp)
GAATATGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTCTTC	212	0.12125995961814554	No Hit
GAATGATCTGTCTCTTATACACATCTGACGCTGTCACTGTCGTATGCCGTC	183	0.10467251231188977	No Hit
GCGATAAGTCGTTTTAAACTGTCATGTCAGAGGTGTAAACAAACATTTTGG	179	0.10238458854550966	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.26368321407530704	0.0
2	0.0	0.0	0.0	1.5643678752623962	0.0
3	0.0	0.0	0.0	2.067139122924424	0.0
4	0.0	0.0	0.0	2.827301794304214	0.0
5	0.0	0.0	0.0	5.3297184137824525	0.0
6	0.0	0.0	0.0	6.620107418020831	0.0
7	0.0	0.0	0.0	7.895052936836144	0.0
8	0.0	0.0	0.0	9.654466313182445	0.0
9	0.0	0.0	0.0	10.282501387053783	0.0
10	0.0	0.0	0.0	12.349068529036613	0.0
11	0.0	0.0	0.0	14.183983389673456	0.0
12	0.0	0.0	0.0	16.429008585433934	0.0
13	0.0	0.0	0.0	17.26638868392905	0.0
14	0.0	0.0	0.0	17.659339590804834	0.0
15	0.0	0.0	0.0	18.18327413330588	0.0
16	0.0	0.0	0.0	19.179092952622817	0.0
17	0.0	0.0	0.0	20.288163998375573	0.0
18	0.0	0.0	0.0	21.630031287357504	0.0
19	0.0	0.0	0.0	22.35301519753362	0.0
20	0.0	0.0	0.0	23.059411660403477	0.0
21	0.0	0.0	0.0	23.847029416979826	0.0
22	0.0	0.0	0.0	24.66896603005188	0.0
23	0.0	0.0	0.0	25.410825311300627	0.0
24	0.0	0.0	0.0	25.979374367246084	0.0
25	0.0	0.0	0.0	26.486149481499275	0.0
26	0.0	0.0	0.0	26.951741967957627	0.0
27	0.0	0.0	0.0	27.44593350149573	0.0
28	0.0	0.0	0.0	27.9206776830196	0.0
29	0.0	0.0	0.0	28.425164873506414	0.0
30	0.0	0.0	0.0	29.006869491108557	0.0
31	0.0	0.0	0.0	29.51250064347856	0.0
32	0.0	0.0	0.0	30.045014900103528	0.0
33	0.0	0.0	0.0	30.513467291269855	0.0
34	0.0	0.0	0.0	30.983063644319373	0.0
35	0.0	0.0	0.0	31.578495804519793	0.0
36	0.0	0.0	0.0	32.03665253873741	0.0
37	0.0	0.0	0.0	32.49652521577981	0.0
38	0.0	0.0	0.0	32.997580520617056	0.0
39	0.0	0.0	0.0	33.506643558636625	0.0
>>END_MODULE
>>Kmer Content	fail
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
TAATACG	30	2.154915E-6	45.000004	4
TCGTTGA	20	7.0168637E-4	45.000004	1
AGATCCC	20	7.0168637E-4	45.000004	14
CTCGTCC	20	7.0168637E-4	45.000004	37
GGTGTCG	20	7.0168637E-4	45.000004	2
CTCCGGT	20	7.0168637E-4	45.000004	20
TTCAGCG	20	7.0168637E-4	45.000004	25
AAATAAC	20	7.0168637E-4	45.000004	28
ACGCATT	20	7.0168637E-4	45.000004	11
CATTGTA	20	7.0168637E-4	45.000004	36
ATGTGCG	20	7.0168637E-4	45.000004	1
CGTTGAG	20	7.0168637E-4	45.000004	2
CGGCACC	20	7.0168637E-4	45.000004	27
GGGTAAC	20	7.0168637E-4	45.000004	7
GCTGCGG	20	7.0168637E-4	45.000004	2
AATGGTC	30	2.154915E-6	45.000004	10
TGTATAA	20	7.0168637E-4	45.000004	43
TAAGTCG	30	2.154915E-6	45.000004	5
AAGCGGC	30	2.154915E-6	45.000004	22
TCTAGTG	30	2.154915E-6	45.000004	14
>>END_MODULE