##FastQC	0.11.5
>>Basic Statistics	pass
#Measure	Value
Filename	SRR2936765.fastq
File type	Conventional base calls
Encoding	Sanger / Illumina 1.9
Total Sequences	331725
Sequences flagged as poor quality	0
Sequence length	51
%GC	48
>>END_MODULE
>>Per base sequence quality	pass
#Base	Mean	Median	Lower Quartile	Upper Quartile	10th Percentile	90th Percentile
1	32.07081166628985	33.0	31.0	34.0	30.0	34.0
2	32.18206948526641	33.0	31.0	34.0	30.0	34.0
3	32.126592810309745	33.0	31.0	34.0	30.0	34.0
4	35.77946491823046	37.0	35.0	37.0	35.0	37.0
5	31.100513979953273	37.0	35.0	37.0	0.0	37.0
6	33.39092923355189	37.0	35.0	37.0	17.0	37.0
7	35.311706986208456	37.0	35.0	37.0	32.0	37.0
8	35.73643530032406	37.0	35.0	37.0	35.0	37.0
9	37.733878966011005	39.0	37.0	39.0	35.0	39.0
10	37.423263245157884	39.0	37.0	39.0	35.0	39.0
11	37.26079131810988	39.0	37.0	39.0	34.0	39.0
12	37.06401989599819	39.0	35.0	39.0	34.0	39.0
13	36.858756500113046	39.0	35.0	39.0	33.0	39.0
14	38.003421508779866	40.0	37.0	41.0	33.0	41.0
15	38.13201296254427	40.0	37.0	41.0	33.0	41.0
16	38.144637877760196	40.0	36.0	41.0	34.0	41.0
17	38.12375310874972	40.0	36.0	41.0	34.0	41.0
18	38.085148843168284	40.0	36.0	41.0	34.0	41.0
19	38.04485040319542	40.0	36.0	41.0	34.0	41.0
20	38.0419172507348	40.0	36.0	41.0	34.0	41.0
21	37.99959605094581	40.0	36.0	41.0	34.0	41.0
22	37.98845730650388	40.0	36.0	41.0	34.0	41.0
23	38.00132639987942	40.0	36.0	41.0	34.0	41.0
24	37.97292636973397	40.0	36.0	41.0	34.0	41.0
25	37.81304695154119	40.0	36.0	41.0	34.0	41.0
26	37.79599366945512	40.0	36.0	41.0	34.0	41.0
27	37.75773909111463	40.0	36.0	41.0	33.0	41.0
28	37.70739317205516	40.0	36.0	41.0	33.0	41.0
29	37.6807415781144	40.0	36.0	41.0	33.0	41.0
30	37.627278619338306	40.0	36.0	41.0	33.0	41.0
31	37.551601477127136	40.0	36.0	41.0	33.0	41.0
32	37.47393172055166	40.0	35.0	41.0	33.0	41.0
33	37.401235963524	39.0	35.0	41.0	33.0	41.0
34	37.25228427161052	39.0	35.0	41.0	33.0	41.0
35	37.188731630115306	39.0	35.0	41.0	32.0	41.0
36	37.07746175295802	39.0	35.0	41.0	32.0	41.0
37	36.958381189238075	39.0	35.0	41.0	31.0	41.0
38	36.94878589192856	39.0	35.0	41.0	32.0	41.0
39	36.8904333408697	39.0	35.0	41.0	31.0	41.0
40	36.80416308689426	39.0	35.0	41.0	31.0	41.0
41	36.8040033159997	39.0	35.0	41.0	31.0	41.0
42	36.74822820107016	39.0	35.0	41.0	31.0	41.0
43	36.67148993895546	39.0	35.0	41.0	31.0	41.0
44	36.49437033687543	39.0	35.0	40.0	31.0	41.0
45	36.487768482930136	39.0	35.0	40.0	31.0	41.0
46	36.38283517974226	38.0	35.0	40.0	31.0	41.0
47	36.27906549099404	38.0	35.0	40.0	30.0	41.0
48	36.15096842263923	38.0	35.0	40.0	30.0	41.0
49	36.06082146356169	38.0	35.0	40.0	30.0	41.0
50	35.82910844826287	38.0	35.0	40.0	29.0	41.0
51	33.966840003014546	36.0	33.0	39.0	24.0	41.0
>>END_MODULE
>>Per sequence quality scores	pass
#Quality	Count
7	2.0
8	7.0
9	17.0
10	24.0
11	11.0
12	13.0
13	4.0
14	12.0
15	13.0
16	18.0
17	28.0
18	83.0
19	145.0
20	246.0
21	364.0
22	589.0
23	824.0
24	1166.0
25	1555.0
26	2006.0
27	2533.0
28	3094.0
29	3938.0
30	5171.0
31	6996.0
32	9378.0
33	14891.0
34	24402.0
35	32521.0
36	26476.0
37	40603.0
38	64879.0
39	89694.0
40	22.0
>>END_MODULE
>>Per base sequence content	fail
#Base	G	A	T	C
1	39.86133092169719	22.5319164970985	27.133921169643532	10.472831411560781
2	30.575928856733743	25.788831110106265	26.227447433868416	17.407792599291582
3	31.885447283141154	25.552490767955383	26.639837214560252	15.922224734343207
4	28.463938503278317	24.076267993066548	29.12593262491522	18.33386087873992
5	22.174089984173637	39.106790263019064	22.1671565302585	16.5519632225488
6	25.671866757103025	35.409450599140854	24.624915215916797	14.293767427839324
7	80.2158414349235	5.336649333031879	9.829226015524908	4.618283216519708
8	80.95048609541035	5.702012208907981	7.826965106639536	5.520536589042129
9	75.75913784007838	6.296480518501771	9.783706383299421	8.160675258120431
10	41.401160599894496	23.357298967518275	18.998869545557316	16.24267088702992
11	32.17695380209511	24.655362122239808	23.829979651820032	19.337704423845054
12	29.405079508629132	21.172356620694853	29.638706760117568	19.783857110558444
13	23.8911749189841	26.076720174843622	30.471625593488582	19.560479312683697
14	18.279900520009043	30.55512849498832	28.687316301153064	22.477654683849575
15	16.929384279146884	25.710452935413368	37.5368151330168	19.82334765242294
16	19.573441856959832	25.811440198959986	32.66892757555204	21.94619036852815
17	20.27944833823197	24.444645414123144	28.21915743462205	27.056748813022836
18	22.521064134448714	25.088552264677066	30.975356093149447	21.415027507724773
19	23.738940387369055	26.26271761248022	27.018765543748586	22.97957645640214
20	24.12480217047253	26.31155324440425	28.312306880699374	21.251337704423847
21	23.144472077775266	27.849272740975206	28.985756274022158	20.02049890722737
22	21.095184264074156	24.988469364684605	27.987338910241917	25.92900746099932
23	19.98703745572387	26.70163539076042	28.456703594845127	24.854623558670585
24	22.154495440500416	24.503127590624764	29.297460245685432	24.044916723189388
25	20.134448715050116	27.791996382545786	28.11575853493104	23.95779636747306
26	18.563870676011756	27.25691461300776	28.96977918456553	25.209435526414953
27	20.222473434320598	28.46484286683247	29.051171904438917	22.26151179440802
28	18.75710302208154	26.40952596277037	31.865249830431836	22.968121184716257
29	19.152611349762605	23.667797121109352	33.81807219835707	23.361519330770967
30	21.288718064661992	25.784309292335518	31.183058256085612	21.743914386916874
31	24.413897053282085	26.34200015072726	27.261134976260458	21.9829678197302
32	25.2486246137614	26.6407415781144	27.812495289773153	20.298138518351045
33	24.561006858090288	24.312909789735475	26.3848066922903	24.74127665988394
34	22.203331072424447	25.93533800587836	28.20529052679177	23.65604039490542
35	21.961866003466728	24.2942196096164	29.58414349235059	24.159770894566282
36	26.06948526641043	24.283970156002713	29.104830808651744	20.541713768935114
37	22.767955384731327	26.18825834652197	31.144170623257217	19.899615645489487
38	22.84000301454518	26.161428894415558	27.110106262717615	23.888461828321653
39	22.367623784761474	25.49702313663426	28.67646393850328	23.45888914010099
40	23.614741125932625	24.02954254276886	29.32881151556259	23.026904815735925
41	21.913633280578793	25.33484060592358	27.41789132564624	25.333634787851384
42	24.184791619564397	22.52317431607506	30.052603813399653	23.239430250960886
43	24.250508704499207	22.602155399804055	28.561609767126384	24.58572612857035
44	21.095787173110256	24.70449920868189	29.22450825231743	24.97520536589042
45	20.82899992463637	24.665008666817396	27.829376742783936	26.676614665762305
46	24.32225487979501	25.304695154118622	27.09985680910393	23.27319315698244
47	20.62943703368754	24.38194287436883	31.20476298138518	23.783857110558447
48	21.7610972944457	22.800813927198735	30.64224885070465	24.795839927650913
49	22.689878664556485	20.651744668023213	32.395508327681064	24.262868339739242
50	21.665536212223984	22.587082673901577	31.183661165121713	24.563719948752734
51	20.96073554902404	22.61903685281483	27.821237470796596	28.59899012736453
>>END_MODULE
>>Per sequence GC content	warn
#GC Content	Count
0	59.0
1	186.0
2	313.0
3	496.0
4	679.0
5	486.5
6	294.0
7	276.0
8	258.0
9	264.5
10	271.0
11	265.5
12	260.0
13	297.0
14	334.0
15	329.0
16	324.0
17	336.0
18	348.0
19	461.0
20	574.0
21	698.0
22	822.0
23	1057.0
24	1292.0
25	1432.5
26	2182.5
27	2792.0
28	2876.0
29	2960.0
30	3733.0
31	4506.0
32	5407.0
33	6308.0
34	7524.5
35	8741.0
36	9443.0
37	10145.0
38	10383.0
39	10621.0
40	13455.5
41	16290.0
42	18803.0
43	21316.0
44	24932.0
45	28548.0
46	34463.0
47	40378.0
48	38485.0
49	36592.0
50	35186.5
51	33781.0
52	28991.0
53	24201.0
54	20804.0
55	17407.0
56	15739.5
57	14072.0
58	13610.5
59	13149.0
60	11637.5
61	10126.0
62	8994.5
63	7863.0
64	7019.5
65	6176.0
66	4823.5
67	3471.0
68	2925.5
69	2380.0
70	1777.0
71	1174.0
72	934.0
73	694.0
74	475.0
75	225.0
76	194.0
77	144.0
78	94.0
79	68.5
80	43.0
81	37.0
82	31.0
83	19.0
84	7.0
85	5.0
86	3.0
87	2.0
88	1.0
89	2.5
90	4.0
91	2.0
92	0.0
93	0.0
94	0.0
95	0.0
96	0.0
97	0.0
98	0.0
99	0.0
100	0.0
>>END_MODULE
>>Per base N content	pass
#Base	N-Count
1	0.0
2	0.0
3	0.0
4	0.0
5	0.0
6	0.0
7	0.0
8	0.0
9	0.0
10	0.0
11	0.0
12	0.0
13	0.0
14	0.0
15	0.0
16	0.0
17	0.0
18	0.0
19	0.0
20	0.0
21	0.0
22	0.0
23	0.0
24	0.0
25	0.0
26	0.0
27	0.0
28	0.0
29	0.0
30	0.0
31	0.0
32	0.0
33	0.0
34	0.0
35	0.0
36	0.0
37	0.0
38	0.0
39	0.0
40	0.0
41	0.0
42	0.0
43	0.0
44	0.0
45	0.0
46	0.0
47	0.0
48	0.0
49	0.0
50	0.0
51	0.0
>>END_MODULE
>>Sequence Length Distribution	pass
#Length	Count
51	331725.0
>>END_MODULE
>>Sequence Duplication Levels	fail
#Total Deduplicated Percentage	30.419943927801647
#Duplication Level	Percentage of deduplicated	Percentage of total
1	71.5091597219225	21.753046290650943
2	11.323228314694797	6.889039408294233
3	4.386545321186204	4.003153881217349
4	2.3346501486890348	2.8407970645661687
5	1.4747765276618627	2.243130963875594
6	1.0228449463573668	1.8668931549015826
7	0.7358884446269929	1.5669979655869204
8	0.5719133020282924	1.3918056463411634
9	0.4829267917462579	1.3221545333538876
>10	5.6041504508022335	42.03368630203676
>50	0.5089228509292303	9.231053620263648
>100	0.037994240344421906	2.20054935560486
>500	0.002999545290349098	0.6597085766837514
>1k	0.003999393720465464	1.997983236623127
>5k	0.0	0.0
>10k+	0.0	0.0
>>END_MODULE
>>Overrepresented sequences	warn
#Sequence	Count	Percentage	Possible Source
GAATGATACGGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGC	1865	0.5622126761624839	No Hit
GAATCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTC	1800	0.5426181324892607	No Hit
GAATGATACCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCG	1704	0.5136784987565002	No Hit
GCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	1200	0.3617454216595071	Illumina Single End Adapter 1 (95% over 21bp)
CCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	979	0.2951239731705479	Illumina Single End Adapter 1 (95% over 21bp)
CTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGCT	631	0.19021780088929083	Illumina Single End Adapter 1 (95% over 22bp)
GAATGCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTT	559	0.1685130755897204	No Hit
TCTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCTTCTGC	391	0.11786871655738941	Illumina Single End Adapter 1 (95% over 21bp)
GAATGACTGTCTCTTATACACATCTGACGCTTGTCAGGTCGTATGCCGTCT	351	0.10581053583540584	No Hit
>>END_MODULE
>>Adapter Content	fail
#Position	Illumina Universal Adapter	Illumina Small RNA 3' Adapter	Illumina Small RNA 5' Adapter	Nextera Transposase Sequence	SOLID Small RNA Adapter
1	0.0	0.0	0.0	0.224583615946944	0.0
2	0.0	0.0	0.0	1.3077096992991182	0.0
3	0.0	0.0	0.0	1.750546386313965	0.0
4	0.0	0.0	0.0	2.3525510588589946	0.0
5	0.0	0.0	0.0	4.372296329791243	0.0
6	0.0	0.0	0.0	5.408696962845731	0.0
7	0.0	0.0	0.0	6.382395056145904	0.0
8	0.0	0.0	0.0	7.806466199412164	0.0
9	0.0	0.0	0.0	8.28668324666516	0.0
10	0.0	0.0	0.0	9.947999095636446	0.0
11	0.0	0.0	0.0	11.526113497626046	0.0
12	0.0	0.0	0.0	13.392719873389103	0.0
13	0.0	0.0	0.0	14.01100308990881	0.0
14	0.0	0.0	0.0	14.293164518803225	0.0
15	0.0	0.0	0.0	14.743537568769312	0.0
16	0.0	0.0	0.0	15.62559348858241	0.0
17	0.0	0.0	0.0	16.605019217725527	0.0
18	0.0	0.0	0.0	17.782802019745272	0.0
19	0.0	0.0	0.0	18.3848066922903	0.0
20	0.0	0.0	0.0	19.059461903685282	0.0
21	0.0	0.0	0.0	19.82455347049514	0.0
22	0.0	0.0	0.0	20.575476674956665	0.0
23	0.0	0.0	0.0	21.324591152309896	0.0
24	0.0	0.0	0.0	21.938654005576907	0.0
25	0.0	0.0	0.0	22.433642324214333	0.0
26	0.0	0.0	0.0	22.929233551887858	0.0
27	0.0	0.0	0.0	23.416685507574044	0.0
28	0.0	0.0	0.0	23.880021101816265	0.0
29	0.0	0.0	0.0	24.397317054789358	0.0
30	0.0	0.0	0.0	24.985756274022158	0.0
31	3.014545180495893E-4	0.0	0.0	25.49792750018841	0.0
32	3.014545180495893E-4	0.0	0.0	26.022156907076646	0.0
33	3.014545180495893E-4	0.0	0.0	26.51774813475017	0.0
34	3.014545180495893E-4	0.0	0.0	27.032632451578866	0.0
35	3.014545180495893E-4	0.0	0.0	27.595146582259403	0.0
36	3.014545180495893E-4	0.0	0.0	28.077775265656793	0.0
37	3.014545180495893E-4	0.0	0.0	28.591453764413295	0.0
38	3.014545180495893E-4	0.0	0.0	29.083427537870225	0.0
39	3.014545180495893E-4	0.0	0.0	29.612178762529204	0.0
>>END_MODULE
>>Kmer Content	warn
#Sequence	Count	PValue	Obs/Exp Max	Max Obs/Exp Position
CTTAAGT	20	7.026024E-4	45.0	16
GCCCCGT	20	7.026024E-4	45.0	10
CGGGTAT	70	0.0	45.0	6
AACCGGC	20	7.026024E-4	45.0	16
CTCACGT	20	7.026024E-4	45.0	45
GTCGACG	20	7.026024E-4	45.0	1
ACGTTAG	25	3.8847866E-5	45.0	1
ACTTCGC	20	7.026024E-4	45.0	14
GGTCATA	25	3.8847866E-5	45.0	8
GGATATC	20	7.026024E-4	45.0	8
TACGGGT	70	0.0	45.0	4
CGCGACT	25	3.8847866E-5	45.0	13
CGTTATT	25	3.8847866E-5	45.0	1
GTAATAC	20	7.026024E-4	45.0	28
TGATTCG	20	7.026024E-4	45.0	34
CTTTGCG	20	7.026024E-4	45.0	1
TCGTACC	20	7.026024E-4	45.0	38
CTATTAC	25	3.8847866E-5	45.0	16
CCGCGAC	25	3.8847866E-5	45.0	12
CGCGTCG	20	7.026024E-4	45.0	14
>>END_MODULE